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Q54C70

- ODPA_DICDI

UniProt

Q54C70 - ODPA_DICDI

Protein

Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial

Gene

pdhA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.By similarity

    Catalytic activityi

    Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

    Cofactori

    Thiamine pyrophosphate.By similarity

    Enzyme regulationi

    E1 activity is regulated by phosphorylation (inactivation) and dephosphorylation (activation) of the alpha subunit.By similarity

    GO - Molecular functioni

    1. pyruvate dehydrogenase (acetyl-transferring) activity Source: dictyBase

    GO - Biological processi

    1. acetyl-CoA biosynthetic process from pyruvate Source: dictyBase
    2. glycolytic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Pyruvate, Thiamine pyrophosphate

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial (EC:1.2.4.1)
    Short name:
    PDHE1-A
    Gene namesi
    Name:pdhA
    ORF Names:DDB_G0292994
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0292994. pdhA.

    Subcellular locationi

    Mitochondrion matrix By similarity

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell
    2. phagocytic vesicle Source: dictyBase
    3. pyruvate dehydrogenase complex Source: dictyBase

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2626MitochondrionSequence AnalysisAdd
    BLAST
    Chaini27 – 377351Pyruvate dehydrogenase E1 component subunit alpha, mitochondrialPRO_0000327983Add
    BLAST

    Proteomic databases

    PRIDEiQ54C70.

    Interactioni

    Subunit structurei

    Tetramer of 2 alpha and 2 beta subunits.By similarity

    Protein-protein interaction databases

    STRINGi44689.DDB_0230193.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54C70.
    SMRiQ54C70. Positions 25-366.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1071.
    KOiK00161.
    OMAiRGPNQWI.
    PhylomeDBiQ54C70.

    Family and domain databases

    Gene3Di3.40.50.970. 1 hit.
    InterProiIPR001017. DH_E1.
    IPR017597. Pyrv_DH_E1_asu_subgrp-y.
    IPR029061. THDP-binding.
    [Graphical view]
    PfamiPF00676. E1_dh. 1 hit.
    [Graphical view]
    SUPFAMiSSF52518. SSF52518. 1 hit.
    TIGRFAMsiTIGR03182. PDH_E1_alph_y. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q54C70-1 [UniParc]FASTAAdd to Basket

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    MLSNFLKVNS KALGHIRTFA SKSGEIKHNF KKADTYLCDG PSDSTVTNKD    50
    ELISFFTEMS RFRRLETVCD GLYKKKLIRG FCHLYTGQEA VCAGLESAIT 100
    KDDHIITAYR DHTYMLSRGA TPEEIFAELL MKETGCSKGK GGSMHMFTKN 150
    FYGGNGIVGA QCPLGAGIAF AQKYNKTGNV CLAMYGDGAA NQGQLFEAFN 200
    MASLWKLPVI FICENNKYGM GTSQKRSTAG HDFYTRGHYV AGLKVDGMDV 250
    FAVKEAGKYA AEWCRAGNGP IILEMDTYRY VGHSMSDPGI TYRTREEVNH 300
    VRQTRDPIEN IRQIILDNKI ATEDQLAAIE ETVRDEMEKA SEKAIAAPLP 350
    QARELFTNVY LQEVPVRGVE FVNSFKP 377
    Length:377
    Mass (Da):41,988
    Last modified:May 24, 2005 - v1
    Checksum:iBD3481894B7348C3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000199 Genomic DNA. Translation: EAL60849.1.
    RefSeqiXP_629349.1. XM_629347.1.

    Genome annotation databases

    EnsemblProtistsiDDB0230193; DDB0230193; DDB_G0292994.
    GeneIDi8629073.
    KEGGiddi:DDB_G0292994.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000199 Genomic DNA. Translation: EAL60849.1 .
    RefSeqi XP_629349.1. XM_629347.1.

    3D structure databases

    ProteinModelPortali Q54C70.
    SMRi Q54C70. Positions 25-366.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0230193.

    Proteomic databases

    PRIDEi Q54C70.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0230193 ; DDB0230193 ; DDB_G0292994 .
    GeneIDi 8629073.
    KEGGi ddi:DDB_G0292994.

    Organism-specific databases

    dictyBasei DDB_G0292994. pdhA.

    Phylogenomic databases

    eggNOGi COG1071.
    KOi K00161.
    OMAi RGPNQWI.
    PhylomeDBi Q54C70.

    Miscellaneous databases

    PROi Q54C70.

    Family and domain databases

    Gene3Di 3.40.50.970. 1 hit.
    InterProi IPR001017. DH_E1.
    IPR017597. Pyrv_DH_E1_asu_subgrp-y.
    IPR029061. THDP-binding.
    [Graphical view ]
    Pfami PF00676. E1_dh. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52518. SSF52518. 1 hit.
    TIGRFAMsi TIGR03182. PDH_E1_alph_y. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
      Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
      Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: AX2.

    Entry informationi

    Entry nameiODPA_DICDI
    AccessioniPrimary (citable) accession number: Q54C70
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

    External Data

    Dasty 3