Q54C64 (PIGL_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase
EC=3.5.1.89

Alternative name(s):
Phosphatidylinositol-glycan biosynthesis class L protein
Short name=PIG-L

Gene names

Name:	pigl
ORF Names:	DDB_G0293136

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the second step of GPI biosynthesis. De-N-acetylation of N-acetylglucosaminyl-phosphatidylinositol By similarity.
Catalytic activity	6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + acetate.
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subcellular location	Endoplasmic reticulum membrane By similarity.
Sequence similarities	Belongs to the PIGL family.

Ontologies

Keywords
Biological process	GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GPI anchor biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	N-acetylglucosaminylphosphatidylinositol deacetylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Probable N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase		PRO_0000328582
Regions
	Compositional bias	148 – 167	20	Ser-rich

Sequences

	Sequence		Length	Mass (Da)
	Q54C64 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: C83B099E30F24125	FASTA	258	29,237
	10 20 30 40 50 60 MKNHLNNNED NSTLIKKKVL FVIAHPDDEC MFFTPTIQHY NFIGSEIFVA CLSNGNAVGL 70 80 90 100 110 120 GKIREKELID SCIDMGINQE NVFFDQTNNF QDGMNIIWDT DLVEKTILSF IKQTSADIVI 130 140 150 160 170 180 SFDECGISSH PNHISISNGL KQLMKNKSSS TTTTSTTSSS SSSSSLSNRT TNNLNKEIKA 190 200 210 220 230 240 YKLETVNIIR KYIGIADIPL TKLLSYDENS TQTFISTQLF PPSSYSPMTK HKSQFVWFRY 250 LFVFLSRYSF INTLIEIK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000199 Genomic DNA. Translation: EAL60920.1.
RefSeq	XP_629355.1. XM_629353.1.
3D structure databases
ProteinModelPortal	Q54C64.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDBDRAFT_0191808.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0235227; DDB0235227; DDB_G0293136.
GeneID	8629079.
KEGG	ddi:DDB_G0293136.
Organism-specific databases
dictyBase	DDB_G0293136. pigL.
Phylogenomic databases
eggNOG	COG2120.
KO	K03434.
OMA	WFRRLYI.
Enzyme and pathway databases
UniPathway	UPA00196.
Family and domain databases
Gene3D	3.40.50.10320. 1 hit.
InterPro	IPR003737. GlcNAc_PIno_de-acetylase. IPR024078. LmbE-like_dom. [Graphical view]
PANTHER	PTHR12993. PTHR12993. 1 hit.
Pfam	PF02585. PIG-L. 1 hit. [Graphical view]
SUPFAM	SSF102588. SSF102588. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PIGL_DICDI

Accession

Primary (citable) accession number: Q54C64

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)