Q54C27 (OST1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
EC=2.4.1.119

Alternative name(s):
Oligosaccharyl transferase subunit alpha

Gene names

Name:	ost1
ORF Names:	DDB_G0293224

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyl transferase (OST) complex By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity.
Induction	Upon infection by L.pneumophila. Ref.2
Sequence similarities	Belongs to the OST1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein N-linked glycosylation Inferred from sequence or structural similarity. Source: dictyBase
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW oligosaccharyltransferase complex Inferred from sequence or structural similarity. Source: dictyBase
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 460	436	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1		PRO_0000327398
Regions
	Topological domain	25 – 425	401	Extracellular Potential
	Transmembrane	426 – 446	21	Helical; Potential
	Topological domain	447 – 460	14	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	23	1	N-linked (GlcNAc...) Potential
	Glycosylation	290	1	N-linked (GlcNAc...) Potential
	Glycosylation	345	1	N-linked (GlcNAc...) Potential
	Glycosylation	420	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q54C27 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 6CDD99F08E524A42	FASTA	460	52,411
	10 20 30 40 50 60 MKVLNNLLLF VFLIFLVGSN VVNSTSQTWI NQDVQRSIDV TTHLSKESIS IKAKSLTDKN 70 80 90 100 110 120 DIYQISFNSK YHVSIQALDS QNNELKVYLS PNSINIKEGF KTYNIELKNK VKKDEIVQLK 130 140 150 160 170 180 VKVTSMIEQM IPYPSEIKQG QTQLVLYLNN HYFTSPYKTE TQKTTVKLAS SKVESYSEEQ 190 200 210 220 230 240 PTSLKGMTVT YGPYSAIEAF SVSPMRIHYE NGSPFFVLTN LLKEYEVSHW GNLAVETHYQ 250 260 270 280 290 300 LENKGAHLVG AFSRLDYQRN PSVNPNQIAE VNEAVPLSAT DFYYRDSIGN ISTSSYQYLA 310 320 330 340 350 360 NRINFKIQPR FPLMGGWKNT FYTGYNLPIE KFLSVDTLTG QYHLNVTFGV GIDNVYVENH 370 380 390 400 410 420 ILKVVLPEGA TDIKVHAPFA FTQSEENRKT YLDTIGRPVV VINIKDTASE NYRYIQVTYN 430 440 450 460 LSSLSIFHEP LLVIGAVFTL CIFIILYSRF DLSISKSKQI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"Dictyostelium transcriptional host cell response upon infection with Legionella." Farbrother P., Wagner C., Na J., Tunggal B., Morio T., Urushihara H., Tanaka Y., Schleicher M., Steinert M., Eichinger L. Cell. Microbiol. 8:438-456(2006) [PubMed: 16469056] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000200 Genomic DNA. Translation: EAL60811.1.
RefSeq	XP_629239.1. XM_629237.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q54C27.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0233146; DDB0233146; DDB_G0293224.
GeneID	8629121.
GenomeReviews	Gene locus ost1 in contig CM000155_GR.
KEGG	ddi:DDB_G0293224.
Organism-specific databases
dictyBase	DDB_G0293224. ost1.
Phylogenomic databases
eggNOG	KOG2291.
GeneTree	EPrGT00050000005905.
HOGENOM	HBG383317.
OMA	AVETHYQ.
PhylomeDB	Q54C27.
ProtClustDB	CLSZ2429298.
Family and domain databases
InterPro	IPR007676. Ribophorin_I. [Graphical view]
KO	K12666.
PANTHER	PTHR21049. Ribophorin_I. 1 hit.
Pfam	PF04597. Ribophorin_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OST1_DICDI

Accession

Primary (citable) accession number: Q54C27

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)