Q54BL8 (LYSG5_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable GH family 25 lysozyme 5
EC=3.2.1.17

Alternative name(s):
1,4-beta-N-acetylmuramidase 5

Gene names

ORF Names:

DDB_G0293566

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 25 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular space Inferred from direct assay PubMed 20422638. Source: dictyBase
Molecular_function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 216	196	Probable GH family 25 lysozyme 5		PRO_0000330651
Sites
	Active site	115	1	By similarity
Amino acid modifications
	Glycosylation	31	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q54BL8 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: C2815F5B580C3D9C	FASTA	216	24,565
	10 20 30 40 50 60 MRFIISLLFV FTLIFNLAFS HIGIDVSSGT NESGFECFKQ KKYSRAIIRC YESIGSIDTN 70 80 90 100 110 120 CKPSIENAKK AGIETIDVYL FPCYDCGNPE NQVTTTSHYL KDYLKDLDFL WLDIEGPGQY 130 140 150 160 170 180 WSGSYDNNKK FIQGLLDSAK TAGFKHVGIY TSESQWPGIV GSWDGGKDYP IWYANYDGAE 190 200 210 NFNDFSPFNG WTKPHMKQYA GNINECGLGI DKNYWE

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000218 Genomic DNA. Translation: EAL60595.1.
RefSeq	XP_629011.1. XM_629009.1.
3D structure databases
ProteinModelPortal	Q54BL8.
ModBase	Search...
Proteomic databases
PRIDE	Q54BL8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0252581; DDB0252581; DDB_G0293566.
GeneID	8629295.
KEGG	ddi:DDB_G0293566.
Organism-specific databases
dictyBase	DDB_G0293566.
Phylogenomic databases
eggNOG	NOG75899.
OMA	AGNINEC.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR002053. Glyco_hydro_25. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF01183. Glyco_hydro_25. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LYSG5_DICDI

Accession

Primary (citable) accession number: Q54BL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)