Q54BK2 (NCSEA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neutral ceramidase A
Short name=N-CDase A
Short name=NCDase A
EC=3.5.1.23

Alternative name(s):
Acylsphingosine deacylase 2A
N-acylsphingosine amidohydrolase 2A

Gene names

Name:	dcd2A
Synonyms:	dcd, rsc25
ORF Names:	DDB_G0293538

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	714 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 3.0. Has no activity toward glycosphingolipids, such as GalCer and Galbeta1-3GalNAcbeta1-4(NeuAcalpha2-3)Galbeta1-4Glcbeta1-1'Cer or sphingomyelin. Ref.1
Catalytic activity	N-acylsphingosine + H2O = a carboxylate + sphingosine.
Subcellular location	Secreted Potential.
Miscellaneous	In contrast to other members of the family, it displays a highest activity at acidic and not neutral pH.
Sequence similarities	Belongs to the neutral ceramidase family.
Biophysicochemical properties	pH dependence: Optimum pH is 3.0. Ref.1

Ontologies

Keywords
Biological process	Lipid metabolism Sphingolipid metabolism
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ceramide metabolic process Inferred by curator. Source: dictyBase
Cellular component	extracellular space Inferred from direct assay. Source: dictyBase
Molecular function	ceramidase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 714	691	Neutral ceramidase A		PRO_0000247108
Sites
	Active site	293	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	218	1	N-linked (GlcNAc...) Potential
	Glycosylation	246	1	N-linked (GlcNAc...) Potential
	Glycosylation	353	1	N-linked (GlcNAc...) Potential
	Glycosylation	373	1	N-linked (GlcNAc...) Potential
	Glycosylation	416	1	N-linked (GlcNAc...) Potential
	Glycosylation	571	1	N-linked (GlcNAc...) Potential
	Glycosylation	610	1	N-linked (GlcNAc...) Potential
	Glycosylation	700	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q54BK2 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: 51D96FD13E4A266F	FASTA	714	78,438
	10 20 30 40 50 60 MKRSIVFIYS LVILLLSVGF IDAFKISIEN HIKLSDDSSY QIGTGIYDIT GPGAETNMMG 70 80 90 100 110 120 YAMPGQITGG IHFRQRARAF VFIDSEGNRA VYVSTDSCMI FQEVKIQVIQ DLQEIFGPTL 130 140 150 160 170 180 YTHDNVLLSG THTHSGPAGF SEYALYGITA LGFYKKNFDT ICDGIVQAIV KAHKSVQPAR 190 200 210 220 230 240 MLTQQGELWN SNINRSPYAY DNNPEEEKAM YDANVDKNMT VIRIEDMSGN PFAAISFFGV 250 260 270 280 290 300 HCTSMNNTNH LISGDNKGYA SYLWEKHANG QSSLPGTGPF IAAFGQSNEG DVSPNTRGPT 310 320 330 340 350 360 CRDGKPCDYK TSTCNGKVEE CWALGPGTDG DMFESTQIIG GNQFNKALEL FNNATIQVSG 370 380 390 400 410 420 KIQYRHTWKP FTNVSVEAPY NSGVEGATTC RGAMGYSFAG GTTDGPGAFN FIQGDNSTTN 430 440 450 460 470 480 PFWNFIGGII AKPTPQQTAC QAPKPILIDV GMVEPIPWVP DVMPLQIITL GQIVLVAVPG 490 500 510 520 530 540 EFTTMSGRRL RNTVREIIGQ SIENPIVLIA GLANTYSGYI ATFEEFQVQR YEGASTVFGP 550 560 570 580 590 600 HTLGAYQQEF ANLAQSIVDG SQADPGTFPR NMSGHTPFFL PPVIVDVAPK FDDFGDIYTD 610 620 630 640 650 660 VSTTTPYSIN QTVTVIFYGA NLRNNFMTES SFLTVDQLQS NGQWTTILND GDWDTKLYWK 670 680 690 700 710 MHDLGFSLIT VDWTISPITQ PGTYRITHSG YAKKNPFSDN LTFYQGISSN FNVQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A neutral ceramidase homologue from Dictyostelium discoideum exhibits an acidic pH optimum." Monjusho H., Okino N., Tani M., Maeda M., Yoshida M., Ito M. Biochem. J. 376:473-479(2003) [PubMed: 12943537] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"A neutral ceramidase homologue of Dictyostelium discoideum exhibits an acidic pH optimum." Iranfar N., Loomis W.F. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-714. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB121061 mRNA. Translation: BAC92751.1. AAFI02000218 Genomic DNA. Translation: EAL60581.1. U82513 mRNA. Translation: AAB69633.1.
RefSeq	XP_629027.1. XM_629025.1.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q54BK2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0215370; DDB0215370; DDB_G0293538.
GeneID	8629313.
GenomeReviews	Gene locus dcd2A in contig CM000155_GR.
KEGG	ddi:DDB_G0293538.
Organism-specific databases
dictyBase	DDB_G0293538. dcd2A.
Phylogenomic databases
eggNOG	KOG2232.
GeneTree	EPrGT00050000005921.
HOGENOM	HBG315824.
OMA	FGLFVRI.
PhylomeDB	Q54BK2.
ProtClustDB	PTZ00487.
Family and domain databases
InterPro	IPR006823. Ceramidase_alk. [Graphical view]
KO	K12349.
PANTHER	PTHR12670. Ceramidase_alk. 1 hit.
Pfam	PF04734. Ceramidase_alk. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCSEA_DICDI

Accession

Primary (citable) accession number: Q54BK2
Secondary accession number(s): O15913, Q75WL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	May 2, 2006
Last modified:	December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)