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Q54BC8

- PSB5_DICDI

UniProt

Q54BC8 - PSB5_DICDI

Protein

Proteasome subunit beta type-5

Gene

psmB5

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity By similarity.By similarity

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei63 – 631NucleophileBy similarity

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. proteolysis Source: dictyBase
    2. proteolysis involved in cellular protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Enzyme and pathway databases

    ReactomeiREACT_204617. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_210566. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_212010. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_214682. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_217211. ER-Phagosome pathway.
    REACT_217636. Autodegradation of the E3 ubiquitin ligase COP1.
    REACT_221036. Separation of Sister Chromatids.
    REACT_225025. APC/C:Cdc20 mediated degradation of Securin.
    REACT_226311. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
    REACT_226712. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_95813. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

    Protein family/group databases

    MEROPSiT01.P01.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit beta type-5 (EC:3.4.25.1)
    Gene namesi
    Name:psmB5
    ORF Names:DDB_G0293784
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0293784. psmB5.

    Subcellular locationi

    Cytoplasm PROSITE-ProRule annotation. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: dictyBase
    2. nucleus Source: dictyBase
    3. proteasome core complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 6262Removed in mature formBy similarityPRO_0000327484Add
    BLAST
    Chaini63 – 272210Proteasome subunit beta type-5PRO_0000327485Add
    BLAST

    Keywords - PTMi

    Zymogen

    Interactioni

    Subunit structurei

    The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.1 Publication

    Protein-protein interaction databases

    STRINGi44689.DDB_0232959.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54BC8.
    SMRiQ54BC8. Positions 63-261.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1B family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    KOiK02737.
    OMAiLRAIMHA.
    PhylomeDBiQ54BC8.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR000243. Pept_T1A_subB.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    [Graphical view]
    PRINTSiPR00141. PROTEASOME.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q54BC8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MINIDFDNIE QSQPTLYDTL ASDVKFLNTP HFELPPNANP GDFLRETFDH    50
    PSAPKALEFA HGTTTLAFVY GGGIIVSVDS KSTQGPYVAS RSVKKVIEIT 100
    PTLLGTMAGG AADCSFWERE LGRRCRLYEL RNKELISVAA ASKILANIVY 150
    SYKGYGLSMG TMITGWDKTG PQLYYVDNDG TRLHGQRFSC GSGSTYAYGV 200
    LDTGFKWDMN DEEAYELGRR AVYHATHRDA YSGGAINVYH VQKDGWKKIS 250
    SDDCFKLYQK YYNIVPINKP AN 272
    Length:272
    Mass (Da):30,280
    Last modified:May 24, 2005 - v1
    Checksum:i9C6D656C754404C4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000219 Genomic DNA. Translation: EAL60569.1.
    RefSeqiXP_628966.1. XM_628964.1.

    Genome annotation databases

    EnsemblProtistsiDDB0232959; DDB0232959; DDB_G0293784.
    GeneIDi8629394.
    KEGGiddi:DDB_G0293784.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000219 Genomic DNA. Translation: EAL60569.1 .
    RefSeqi XP_628966.1. XM_628964.1.

    3D structure databases

    ProteinModelPortali Q54BC8.
    SMRi Q54BC8. Positions 63-261.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0232959.

    Protein family/group databases

    MEROPSi T01.P01.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0232959 ; DDB0232959 ; DDB_G0293784 .
    GeneIDi 8629394.
    KEGGi ddi:DDB_G0293784.

    Organism-specific databases

    dictyBasei DDB_G0293784. psmB5.

    Phylogenomic databases

    eggNOGi COG0638.
    KOi K02737.
    OMAi LRAIMHA.
    PhylomeDBi Q54BC8.

    Enzyme and pathway databases

    Reactomei REACT_204617. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_210566. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_212010. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_214682. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_217211. ER-Phagosome pathway.
    REACT_217636. Autodegradation of the E3 ubiquitin ligase COP1.
    REACT_221036. Separation of Sister Chromatids.
    REACT_225025. APC/C:Cdc20 mediated degradation of Securin.
    REACT_226311. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
    REACT_226712. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_95813. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

    Miscellaneous databases

    PROi Q54BC8.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR000243. Pept_T1A_subB.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    [Graphical view ]
    PRINTSi PR00141. PROTEASOME.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "Proteasomes from Dictyostelium discoideum: characterization of structure and function."
      Schauer T.M., Nesper M., Kehl M., Lottspeich F., Mueller-Taubenberger A., Gerisch G., Baumeister W.
      J. Struct. Biol. 111:135-147(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE PROTEASOME COMPLEX.

    Entry informationi

    Entry nameiPSB5_DICDI
    AccessioniPrimary (citable) accession number: Q54BC8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3