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Protein

Proteasome subunit beta type-5

Gene

psmB5

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity (By similarity).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei63 – 631NucleophileBy similarity

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. proteolysis Source: dictyBase
  2. proteolysis involved in cellular protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Protein family/group databases

MEROPSiT01.A06.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-5 (EC:3.4.25.1)
Gene namesi
Name:psmB5
ORF Names:DDB_G0293784
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0293784. psmB5.

Subcellular locationi

Cytoplasm PROSITE-ProRule annotation. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: dictyBase
  2. nucleus Source: dictyBase
  3. proteasome core complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 6262Removed in mature formBy similarityPRO_0000327484Add
BLAST
Chaini63 – 272210Proteasome subunit beta type-5PRO_0000327485Add
BLAST

Keywords - PTMi

Zymogen

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.1 Publication

Protein-protein interaction databases

STRINGi44689.DDB_0232959.

Structurei

3D structure databases

ProteinModelPortaliQ54BC8.
SMRiQ54BC8. Positions 63-261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
InParanoidiQ54BC8.
KOiK02737.
OMAiCNLCHIT.
PhylomeDBiQ54BC8.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54BC8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MINIDFDNIE QSQPTLYDTL ASDVKFLNTP HFELPPNANP GDFLRETFDH
60 70 80 90 100
PSAPKALEFA HGTTTLAFVY GGGIIVSVDS KSTQGPYVAS RSVKKVIEIT
110 120 130 140 150
PTLLGTMAGG AADCSFWERE LGRRCRLYEL RNKELISVAA ASKILANIVY
160 170 180 190 200
SYKGYGLSMG TMITGWDKTG PQLYYVDNDG TRLHGQRFSC GSGSTYAYGV
210 220 230 240 250
LDTGFKWDMN DEEAYELGRR AVYHATHRDA YSGGAINVYH VQKDGWKKIS
260 270
SDDCFKLYQK YYNIVPINKP AN
Length:272
Mass (Da):30,280
Last modified:May 24, 2005 - v1
Checksum:i9C6D656C754404C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000219 Genomic DNA. Translation: EAL60569.1.
RefSeqiXP_628966.1. XM_628964.1.

Genome annotation databases

EnsemblProtistsiDDB0232959; DDB0232959; DDB_G0293784.
GeneIDi8629394.
KEGGiddi:DDB_G0293784.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000219 Genomic DNA. Translation: EAL60569.1.
RefSeqiXP_628966.1. XM_628964.1.

3D structure databases

ProteinModelPortaliQ54BC8.
SMRiQ54BC8. Positions 63-261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB_0232959.

Protein family/group databases

MEROPSiT01.A06.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0232959; DDB0232959; DDB_G0293784.
GeneIDi8629394.
KEGGiddi:DDB_G0293784.

Organism-specific databases

dictyBaseiDDB_G0293784. psmB5.

Phylogenomic databases

eggNOGiCOG0638.
InParanoidiQ54BC8.
KOiK02737.
OMAiCNLCHIT.
PhylomeDBiQ54BC8.

Miscellaneous databases

PROiQ54BC8.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "Proteasomes from Dictyostelium discoideum: characterization of structure and function."
    Schauer T.M., Nesper M., Kehl M., Lottspeich F., Mueller-Taubenberger A., Gerisch G., Baumeister W.
    J. Struct. Biol. 111:135-147(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE PROTEASOME COMPLEX.

Entry informationi

Entry nameiPSB5_DICDI
AccessioniPrimary (citable) accession number: Q54BC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: January 7, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.