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Q54BC8 (PSB5_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome subunit beta type-5

EC=3.4.25.1
Gene names
Name:psmB5
ORF Names:DDB_G0293784
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length272 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity By similarity.

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Ref.2

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Sequence similarities

Belongs to the peptidase T1B family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 6262Removed in mature form By similarity
PRO_0000327484
Chain63 – 272210Proteasome subunit beta type-5
PRO_0000327485

Sites

Active site631Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54BC8 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 9C6D656C754404C4

FASTA27230,280
        10         20         30         40         50         60 
MINIDFDNIE QSQPTLYDTL ASDVKFLNTP HFELPPNANP GDFLRETFDH PSAPKALEFA 

        70         80         90        100        110        120 
HGTTTLAFVY GGGIIVSVDS KSTQGPYVAS RSVKKVIEIT PTLLGTMAGG AADCSFWERE 

       130        140        150        160        170        180 
LGRRCRLYEL RNKELISVAA ASKILANIVY SYKGYGLSMG TMITGWDKTG PQLYYVDNDG 

       190        200        210        220        230        240 
TRLHGQRFSC GSGSTYAYGV LDTGFKWDMN DEEAYELGRR AVYHATHRDA YSGGAINVYH 

       250        260        270 
VQKDGWKKIS SDDCFKLYQK YYNIVPINKP AN 

« Hide

References

« Hide 'large scale' references
[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"Proteasomes from Dictyostelium discoideum: characterization of structure and function."
Schauer T.M., Nesper M., Kehl M., Lottspeich F., Mueller-Taubenberger A., Gerisch G., Baumeister W.
J. Struct. Biol. 111:135-147(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE PROTEASOME COMPLEX.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000219 Genomic DNA. Translation: EAL60569.1.
RefSeqXP_628966.1. XM_628964.1.

3D structure databases

ProteinModelPortalQ54BC8.
SMRQ54BC8. Positions 63-261.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0232959.

Protein family/group databases

MEROPST01.P01.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0232959; DDB0232959; DDB_G0293784.
GeneID8629394.
KEGGddi:DDB_G0293784.

Organism-specific databases

dictyBaseDDB_G0293784. psmB5.

Phylogenomic databases

eggNOGCOG0638.
OMALRAIMHA.
PhylomeDBQ54BC8.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSPR00141. PROTEASOME.
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ54BC8.

Entry information

Entry namePSB5_DICDI
AccessionPrimary (citable) accession number: Q54BC8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: July 9, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase