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Protein

Isocitrate dehydrogenase [NAD] regulatory subunit B, mitochondrial

Gene

idhB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Performs an essential role in the oxidative function of the citric acid cycle.By similarity

Catalytic activityi

Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011SubstrateBy similarity
Binding sitei103 – 1031SubstrateBy similarity
Binding sitei107 – 1071SubstrateBy similarity
Binding sitei140 – 1401SubstrateBy similarity
Sitei147 – 1471Critical for catalysisBy similarity
Sitei194 – 1941Critical for catalysisBy similarity
Metal bindingi227 – 2271Magnesium or manganeseBy similarity
Binding sitei297 – 2971NADP; via amide nitrogen and carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi284 – 2907NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD

Enzyme and pathway databases

ReactomeiREACT_350347. Citric acid cycle (TCA cycle).

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NAD] regulatory subunit B, mitochondrial (EC:1.1.1.41)
Gene namesi
Name:idhB
ORF Names:DDB_G0293872
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0293872. idhB.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: UniProtKB
  • phagocytic vesicle Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 113113MitochondrionSequence AnalysisAdd
BLAST
Chaini114 – 360247Isocitrate dehydrogenase [NAD] regulatory subunit B, mitochondrialPRO_0000328019Add
BLAST

Proteomic databases

PRIDEiQ54B68.

Interactioni

Subunit structurei

Heterooligomer of catalytic and regulatory subunits.

Protein-protein interaction databases

STRINGi44689.DDB0231294.

Structurei

3D structure databases

ProteinModelPortaliQ54B68.
SMRiQ54B68. Positions 29-357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0473.
InParanoidiQ54B68.
KOiK00030.
OMAiKGQNTAN.
PhylomeDBiQ54B68.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54B68-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGRLRTVVK ASSSNSIRNY LGYTSGVQKK TVTVIPGDGI GPEITSSVMG
60 70 80 90 100
VFQAAKVPIE WEIFDISGGQ PISQELIASI TRNKVALKGP LYTEILSGSQ
110 120 130 140 150
SRNMELRKAL DLYAHVVPCK QIPGITARHD DVLVDFVVIR ENTQGEYSGL
160 170 180 190 200
EQVLTPGVVQ SLKIITKEAS ERIARYAFEY AKANGRKKVT AVHKANIQKQ
210 220 230 240 250
TDGLFLATCT QIAKEYPEIK FENTIIDNCC MQLVKSPEQY DVMVTPNLYG
260 270 280 290 300
NIVSNIGAAL VGGPGLAGGA NVGEGSIIFE MGAHHVAADI AGKDKANPTG
310 320 330 340 350
LLLASVMMLK HLGLNEHATK VENAVKAVIK EGTLTSDIGG KSSTKQFTGA
360
VIDYIEKNQN
Length:360
Mass (Da):38,688
Last modified:May 24, 2005 - v1
Checksum:i23CA6A3540462C19
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000223 Genomic DNA. Translation: EAL60507.1.
RefSeqiXP_628920.1. XM_628918.1.

Genome annotation databases

EnsemblProtistsiDDB0231294; DDB0231294; DDB_G0293872.
GeneIDi8629462.
KEGGiddi:DDB_G0293872.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000223 Genomic DNA. Translation: EAL60507.1.
RefSeqiXP_628920.1. XM_628918.1.

3D structure databases

ProteinModelPortaliQ54B68.
SMRiQ54B68. Positions 29-357.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0231294.

Proteomic databases

PRIDEiQ54B68.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0231294; DDB0231294; DDB_G0293872.
GeneIDi8629462.
KEGGiddi:DDB_G0293872.

Organism-specific databases

dictyBaseiDDB_G0293872. idhB.

Phylogenomic databases

eggNOGiCOG0473.
InParanoidiQ54B68.
KOiK00030.
OMAiKGQNTAN.
PhylomeDBiQ54B68.

Enzyme and pathway databases

ReactomeiREACT_350347. Citric acid cycle (TCA cycle).

Miscellaneous databases

PROiQ54B68.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiIDHB_DICDI
AccessioniPrimary (citable) accession number: Q54B68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: June 24, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.