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Q54B68

- IDHB_DICDI

UniProt

Q54B68 - IDHB_DICDI

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Protein
Isocitrate dehydrogenase [NAD] regulatory subunit B, mitochondrial
Gene
idhB, DDB_G0293872
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Performs an essential role in the oxidative function of the citric acid cycle By similarity.

Catalytic activityi

Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

Cofactori

Binds 1 magnesium or manganese ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011Substrate By similarity
Binding sitei103 – 1031Substrate By similarity
Binding sitei107 – 1071Substrate By similarity
Binding sitei140 – 1401Substrate By similarity
Sitei147 – 1471Critical for catalysis By similarity
Sitei194 – 1941Critical for catalysis By similarity
Metal bindingi227 – 2271Magnesium or manganese By similarity
Binding sitei297 – 2971NADP; via amide nitrogen and carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi284 – 2907NADP By similarity

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. isocitrate dehydrogenase (NAD+) activity Source: UniProtKB
  3. magnesium ion binding Source: InterPro

GO - Biological processi

  1. isocitrate metabolic process Source: UniProtKB
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NAD] regulatory subunit B, mitochondrial (EC:1.1.1.41)
Gene namesi
Name:idhB
ORF Names:DDB_G0293872
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0293872. idhB.

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB
  2. phagocytic vesicle Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 113113Mitochondrion Reviewed prediction
Add
BLAST
Chaini114 – 360247Isocitrate dehydrogenase [NAD] regulatory subunit B, mitochondrial
PRO_0000328019Add
BLAST

Proteomic databases

PRIDEiQ54B68.

Interactioni

Subunit structurei

Heterooligomer of catalytic and regulatory subunits.

Protein-protein interaction databases

STRINGi44689.DDB_0231294.

Structurei

3D structure databases

ProteinModelPortaliQ54B68.
SMRiQ54B68. Positions 29-357.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0473.
KOiK00030.
OMAiCAVHKAN.
PhylomeDBiQ54B68.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54B68-1 [UniParc]FASTAAdd to Basket

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MLGRLRTVVK ASSSNSIRNY LGYTSGVQKK TVTVIPGDGI GPEITSSVMG    50
VFQAAKVPIE WEIFDISGGQ PISQELIASI TRNKVALKGP LYTEILSGSQ 100
SRNMELRKAL DLYAHVVPCK QIPGITARHD DVLVDFVVIR ENTQGEYSGL 150
EQVLTPGVVQ SLKIITKEAS ERIARYAFEY AKANGRKKVT AVHKANIQKQ 200
TDGLFLATCT QIAKEYPEIK FENTIIDNCC MQLVKSPEQY DVMVTPNLYG 250
NIVSNIGAAL VGGPGLAGGA NVGEGSIIFE MGAHHVAADI AGKDKANPTG 300
LLLASVMMLK HLGLNEHATK VENAVKAVIK EGTLTSDIGG KSSTKQFTGA 350
VIDYIEKNQN 360
Length:360
Mass (Da):38,688
Last modified:May 24, 2005 - v1
Checksum:i23CA6A3540462C19
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000223 Genomic DNA. Translation: EAL60507.1.
RefSeqiXP_628920.1. XM_628918.1.

Genome annotation databases

EnsemblProtistsiDDB0231294; DDB0231294; DDB_G0293872.
GeneIDi8629462.
KEGGiddi:DDB_G0293872.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000223 Genomic DNA. Translation: EAL60507.1 .
RefSeqi XP_628920.1. XM_628918.1.

3D structure databases

ProteinModelPortali Q54B68.
SMRi Q54B68. Positions 29-357.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231294.

Proteomic databases

PRIDEi Q54B68.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231294 ; DDB0231294 ; DDB_G0293872 .
GeneIDi 8629462.
KEGGi ddi:DDB_G0293872.

Organism-specific databases

dictyBasei DDB_G0293872. idhB.

Phylogenomic databases

eggNOGi COG0473.
KOi K00030.
OMAi CAVHKAN.
PhylomeDBi Q54B68.

Miscellaneous databases

PROi Q54B68.

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view ]
PANTHERi PTHR11835. PTHR11835. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00175. mito_nad_idh. 1 hit.
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiIDHB_DICDI
AccessioniPrimary (citable) accession number: Q54B68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: September 3, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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