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Q54B68

- IDHB_DICDI

UniProt

Q54B68 - IDHB_DICDI

Protein

Isocitrate dehydrogenase [NAD] regulatory subunit B, mitochondrial

Gene

idhB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Performs an essential role in the oxidative function of the citric acid cycle.By similarity

    Catalytic activityi

    Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei101 – 1011SubstrateBy similarity
    Binding sitei103 – 1031SubstrateBy similarity
    Binding sitei107 – 1071SubstrateBy similarity
    Binding sitei140 – 1401SubstrateBy similarity
    Sitei147 – 1471Critical for catalysisBy similarity
    Sitei194 – 1941Critical for catalysisBy similarity
    Metal bindingi227 – 2271Magnesium or manganeseBy similarity
    Binding sitei297 – 2971NADP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi284 – 2907NADPBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NAD+) activity Source: UniProtKB
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. isocitrate metabolic process Source: UniProtKB
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NAD] regulatory subunit B, mitochondrial (EC:1.1.1.41)
    Gene namesi
    Name:idhB
    ORF Names:DDB_G0293872
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0293872. idhB.

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB
    2. phagocytic vesicle Source: dictyBase

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 113113MitochondrionSequence AnalysisAdd
    BLAST
    Chaini114 – 360247Isocitrate dehydrogenase [NAD] regulatory subunit B, mitochondrialPRO_0000328019Add
    BLAST

    Proteomic databases

    PRIDEiQ54B68.

    Interactioni

    Subunit structurei

    Heterooligomer of catalytic and regulatory subunits.

    Protein-protein interaction databases

    STRINGi44689.DDB_0231294.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54B68.
    SMRiQ54B68. Positions 29-357.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0473.
    KOiK00030.
    OMAiCAVHKAN.
    PhylomeDBiQ54B68.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11835. PTHR11835. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q54B68-1 [UniParc]FASTAAdd to Basket

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    MLGRLRTVVK ASSSNSIRNY LGYTSGVQKK TVTVIPGDGI GPEITSSVMG    50
    VFQAAKVPIE WEIFDISGGQ PISQELIASI TRNKVALKGP LYTEILSGSQ 100
    SRNMELRKAL DLYAHVVPCK QIPGITARHD DVLVDFVVIR ENTQGEYSGL 150
    EQVLTPGVVQ SLKIITKEAS ERIARYAFEY AKANGRKKVT AVHKANIQKQ 200
    TDGLFLATCT QIAKEYPEIK FENTIIDNCC MQLVKSPEQY DVMVTPNLYG 250
    NIVSNIGAAL VGGPGLAGGA NVGEGSIIFE MGAHHVAADI AGKDKANPTG 300
    LLLASVMMLK HLGLNEHATK VENAVKAVIK EGTLTSDIGG KSSTKQFTGA 350
    VIDYIEKNQN 360
    Length:360
    Mass (Da):38,688
    Last modified:May 24, 2005 - v1
    Checksum:i23CA6A3540462C19
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000223 Genomic DNA. Translation: EAL60507.1.
    RefSeqiXP_628920.1. XM_628918.1.

    Genome annotation databases

    EnsemblProtistsiDDB0231294; DDB0231294; DDB_G0293872.
    GeneIDi8629462.
    KEGGiddi:DDB_G0293872.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000223 Genomic DNA. Translation: EAL60507.1 .
    RefSeqi XP_628920.1. XM_628918.1.

    3D structure databases

    ProteinModelPortali Q54B68.
    SMRi Q54B68. Positions 29-357.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0231294.

    Proteomic databases

    PRIDEi Q54B68.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0231294 ; DDB0231294 ; DDB_G0293872 .
    GeneIDi 8629462.
    KEGGi ddi:DDB_G0293872.

    Organism-specific databases

    dictyBasei DDB_G0293872. idhB.

    Phylogenomic databases

    eggNOGi COG0473.
    KOi K00030.
    OMAi CAVHKAN.
    PhylomeDBi Q54B68.

    Miscellaneous databases

    PROi Q54B68.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11835. PTHR11835. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00175. mito_nad_idh. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiIDHB_DICDI
    AccessioniPrimary (citable) accession number: Q54B68
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3