Reviewed,
UniProtKB/Swiss-Prot Q54B68 (IDHB_DICDI)
Last modified
June 16, 2009.
Version 27.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Isocitrate dehydrogenase [NAD] regulatory subunit B, mitochondrial EC=1.1.1.41 | ||||
| Gene names |
| ||||
| Organism | Dictyostelium discoideum (Slime mold) [Complete proteome] | ||||
| Taxonomic identifier | 44689 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium |
Protein attributes
| Sequence length | 360 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Performs an essential role in the oxidative function of the citric acid cycle By similarity. |
| Catalytic activity | Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH. |
| Cofactor | Binds 1 magnesium or manganese ion per subunit By similarity. |
| Subunit structure | Heteroligomer of catalytic and regulatory subunits. |
| Subcellular location | Mitochondrion By similarity. |
| Sequence similarities | Belongs to the isocitrate and isopropylmalate dehydrogenases family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Tricarboxylic acid cycle |
| Cellular component | Mitochondrion |
| Domain | Transit peptide |
| Ligand | Magnesium Manganese Metal-binding NAD |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | isocitrate metabolic process Inferred from sequence or structural similarity. Source: UniProtKB oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycleInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB |
| Molecular function | NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NAD+) activityInferred from sequence or structural similarity. Source: UniProtKB magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 113 | 113 | Mitochondrion Potential | ||||||
| Chain | 114 – 360 | 247 | Isocitrate dehydrogenase [NAD] regulatory subunit B, mitochondrial | PRO_0000328019 | |||||
Regions | |||||||||
| Nucleotide binding | 284 – 290 | 7 | NADP By similarity | ||||||
Sites | |||||||||
| Metal binding | 227 | 1 | Magnesium or manganese By similarity | ||||||
| Binding site | 101 | 1 | Substrate By similarity | ||||||
| Binding site | 103 | 1 | Substrate By similarity | ||||||
| Binding site | 107 | 1 | Substrate By similarity | ||||||
| Binding site | 140 | 1 | Substrate By similarity | ||||||
| Binding site | 297 | 1 | NADP; via amide nitrogen and carbonyl oxygen By similarity | ||||||
| Site | 147 | 1 | Critical for catalysis By similarity | ||||||
| Site | 194 | 1 | Critical for catalysis By similarity | ||||||
Sequences
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References
| [1] | "The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. Kuspa A.Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4. |
Cross-references
Sequence databases | |
|---|---|
| AAFI02000223 Genomic DNA. Translation: EAL60507.1. | |
| RefSeq | XP_628920.1. |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 3385582. |
| KEGG | ddi:DDB_0231294. |
Organism-specific databases | |
| dictyBase | DDB_G0293872. idhB. |
Phylogenomic databases | |
| OMA | Q54B68. MENMATE. |
Enzyme and pathway databases | |
| BRENDA | 1.1.1.41. 424. |
Family and domain databases | |
| InterPro | IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004434. Isocitrate_DH_NAD_mit. [Graphical view] |
| Gene3D | G3DSA:3.40.718.10. IDH_IMDH. 1 hit. |
| PANTHER | PTHR11835. IDH_IMDH_dimeric. 1 hit. |
| Pfam | PF00180. Iso_dh. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00175. mito_nad_idh. 1 hit. |
| PROSITE | PS00470. IDH_IMDH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | IDHB_DICDI | ||||||||
| Accession | Primary (citable) accession number: Q54B68 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
Relevant documents
| Dictyostelium discoideum Dictyostelium discoideum: entries, gene names and cross-references to dictyBase |
| SIMILARITY comments Index of protein domains and families |

Clusters with


