Reviewed, UniProtKB/Swiss-Prot Q54975 (PURK_SYNE7)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase ATPase subunit
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:	purK
Ordered Locus Names:	Synpcc7942_1428

Organism

Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) [Complete proteome] [HAMAP]

Taxonomic identifier

1140 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses an ATPase activity that is dependent on the presence of AIR (aminoimidazole ribonucleotide). The association of purK and purE produces an enzyme complex capable of converting AIR to CAIR efficiently under physiological condition By similarity.
Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purK/purT family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Phosphoribosylaminoimidazole carboxylase ATPase subunit		PRO_0000075013
Regions
	Domain	113 – 298	186	ATP-grasp
	Nucleotide binding	184 – 187	4	ATP By similarity
	Nucleotide binding	268 – 269	2	ATP By similarity
Sites
	Binding site	109	1	ATP By similarity
	Binding site	149	1	ATP By similarity
	Binding site	192	1	ATP By similarity
Experimental info
	Sequence conflict	59	1	A → R in AAB05791. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q54975-1 [UniParc]. Last modified April 18, 2006. Version 2. Checksum: 305196BD699FF228	FASTA	395	42,459
	10 20 30 40 50 60 MNAIAVSPVQ HVGVIGGGQL AWMLAPAAQQ LGMSLHVQTP NDHDPAVAIA DQTVLAAVAD 70 80 90 100 110 120 AAATAKLAQA CDVITFENEF VDLPALTELE ETGVRFRPRP AAIASLLDKL DQRQLLTRLG 130 140 150 160 170 180 LPTPRFLAIA AATATESELT ALGFPVVLKQ RRHGYDGKGT QVLRSLAELQ QALQSYGDTP 190 200 210 220 230 240 LLLEEFIPFE QELAVMVARS QSGAIATFPV VQTHQQNQVC RWVVAPAAIP GALQKAVAAI 250 260 270 280 290 300 ARTLVETVDY VGVAGIELFQ QGDRLWVNEI APRTHNSGHY SLDACQTSQF EQQLRAIADL 310 320 330 340 350 360 PLGSTALQWP GALMVNLLGF EDHQSGYAEL RQQLAALPGA CLYWYGKTES KPGRKLGHIT 370 380 390 LPLSGASSTE RAQQAQTMLA QVEAIWPNPD TAHQP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Lieman-Hurwitz J., Bonfil D., Ronen-Tarazi M., Kaplan A. Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U62615 Genomic DNA. Translation: AAB05791.1. CP000100 Genomic DNA. Translation: ABB57458.1.
RefSeq	YP_400445.1.
3D structure databases
HSSP	HSSP built from PDB template 1B6S based on UniProtKB P09029.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54975.
Genome annotation databases
GeneID	3773600.
GenomeReviews	Gene locus Synpcc7942_1428 in contig CP000100_GR.
KEGG	syf:Synpcc7942_1428.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q54975.
Enzyme and pathway databases
BioCyc	SELO1140:SYNPCC7942_1428-MON.
Family and domain databases
InterPro	IPR005875. AIR_COase_ATPase-su. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PANTHER	PTHR23047:SF1. PurK_ATP. 1 hit.
Pfam	PF02222. ATP-grasp. 1 hit. [Graphical view]
TIGRFAMs	TIGR01161. purK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURK_SYNE7

Accession

Primary (citable) accession number: Q54975
Secondary accession number(s): Q31NB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	April 18, 2006
Last modified:	September 1, 2009
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)