Reviewed, UniProtKB/Swiss-Prot Q54970 (POXB_STRPN)

Names and origin

Protein names

Recommended name:
    Pyruvate oxidase
    EC=1.2.3.3
Alternative name(s):
    Pyruvic oxidase
      Short name=POX

Gene names

Name:	spxB
Ordered Locus Names:	SP_0730

Organism

Streptococcus pneumoniae [Complete proteome] [HAMAP]

Taxonomic identifier

1313 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Important for the aerobic growth. Decarboxylates pyruvate in four steps. The energy released is partially stored in acetyl phosphate By similarity.
Catalytic activity	Pyruvate + phosphate + O2 = acetyl phosphate + CO2 + H2O2.
Cofactor	Binds 1 FAD per subunit By similarity. Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Ligand	FAD Flavoprotein Magnesium Metal-binding Thiamine pyrophosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pyruvate oxidase activity Inferred from electronic annotation. Source: EC thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	Pyruvate oxidase		PRO_0000090819
Regions
	Domain	189 – 336	148	FAD-binding
	Region	1 – 188	188	Core
	Region	337 – 591	255	Thiamine pyrophosphate binding
Sites
	Metal binding	441	1	Magnesium By similarity
	Metal binding	468	1	Magnesium By similarity
	Metal binding	470	1	Magnesium By similarity
Experimental info
	Sequence conflict	152	1	V → I in AAB40976. Ref.1
	Sequence conflict	282	1	P → A in AAB40976. Ref.1
	Sequence conflict	386	1	I → L in AAB40976. Ref.1
	Sequence conflict	390	1	N → S in AAB40976. Ref.1
	Sequence conflict	430	1	N → T in AAB40976. Ref.1
	Sequence conflict	496	1	A → G in AAB40976. Ref.1
	Sequence conflict	528	1	E → G in AAB40976. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q54970-1 [UniParc]. Last modified September 26, 2001. Version 2. Checksum: DCF1595F7485B6FD	FASTA	591	65,266
	10 20 30 40 50 60 MTQGKITASA AMLNVLKTWG VDTIYGIPSG TLSSLMDALA EDKDIRFLQV RHEETGALAA 70 80 90 100 110 120 VMQAKFGGSI GVAVGSGGPG ATHLINGVYD AAMDNTPFLA ILGSRPVNEL NMDAFQELNQ 130 140 150 160 170 180 NPMYNGIAVY NKRVAYAEQL PKVIDEACRA AVSKKGPAVV EIPVNFGFQE IDENSYYGSG 190 200 210 220 230 240 SYERSFIAPA LNEVEIDKAV EILNNAERPV IYAGFGGVKA GEVITELSRK IKAPIITTGK 250 260 270 280 290 300 NFEAFEWNYE GLTGSAYRVG WKPANEVVFE ADTVLFLGSN FPFAEVYEAF KNTEKFIQVD 310 320 330 340 350 360 IDPYKLGKRH ALDASILGDA GQAAKAILDK VNPVESTPWW RANVKNNQNW RDYMNKLEGK 370 380 390 400 410 420 TEGELQLYQV YNAINKHADQ DAIYSIDVGN TTQTSTRHLH MTPKNMWRTS PLFATMGIAL 430 440 450 460 470 480 PGGIAAKKDN PDRQVWNIMG DGAFNMCYPD VITNVQYDLP VINLVFSNAE YGFIKNKYED 490 500 510 520 530 540 TNKHLFGVDF TNADYAKIAE AQGAVGFTVD RIEDIDAVVA EAVKLNKEGK TVVIDARITQ 550 560 570 580 590 HRPLPVEVLE LDPKLHSEEA IKAFKEKYEA EELVPFRLFL EEEGLQSRAI K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pyruvate oxidase, as a determinant of virulence in Streptococcus pneumoniae." Spellerberg B., Cundell D.R., Sandros J., Pearce B.J., Idanpaan-Heikkila I., Rosenow C., Masure H.R. Mol. Microbiol. 19:803-813(1996) [PubMed: 8820650] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: R6x.
[2]	"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae." Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. , Lewis M.R., Radune D., Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M. Science 293:498-506(2001) [PubMed: 11463916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-334 / TIGR4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L39074 Genomic DNA. Translation: AAB40976.1. AE005672 Genomic DNA. Translation: AAK74871.1.
PIR	F95084.
RefSeq	NP_345231.1.
3D structure databases
HSSP	HSSP built from PDB template 1POX based on UniProtKB P37063.
ModBase	Search...
Genome annotation databases
GeneID	930680.
GenomeReviews	Gene locus SP_0730 in contig AE005672_GR.
KEGG	spn:SP_0730.
TIGR	SP_0730.
Phylogenomic databases
HOGENOM	Q54970.
OMA	Q54970. IYAGSGC.
Enzyme and pathway databases
BRENDA	1.2.3.3. 600.
Family and domain databases
InterPro	IPR014092. Pyruvate_oxidase. IPR000399. TPP_bd_CS. IPR012001. TPP_bd_enzyme_N. IPR011766. TPP_enzyme_bd_C. IPR012000. TPP_enzyme_M. [Graphical view]
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02720. pyruv_oxi_spxB. 1 hit.
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POXB_STRPN

Accession

Primary (citable) accession number: Q54970

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	September 26, 2001
Last modified:	June 16, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)