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Protein

Toxin zeta

Gene
N/A
Organism
Streptococcus pyogenes
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Toxic component of a toxin-antitoxin (TA) module. Phosphorylates UDP-N-acetyl-D-glucosamine (UNAG) on the 3'-hydroxyl group of the N-acetyl-D-glucosamine moiety, yielding UNAG-3P. UNAG-3P inhibits MurA, the first committed step in cell wall synthesis, which is then blocked. Phosphorylation is inhibited by cognate epsilon antitoxin. Part of a postsegregational killing (PSK) system involved in the killing of plasmid-free cells. The zeta toxin induces programmed cell death.1 Publication

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-glucosamine = ADP + UDP-N-acetyl-alpha-D-glucosamine 3'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei66Substrate1
Active sitei67Proton acceptorCurated1
Binding sitei100Substrate1
Binding sitei118Substrate1
Binding sitei120Substrate1
Binding sitei128Substrate1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi40 – 47ATPSequence analysis8

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Toxin, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.176. 5935.

Names & Taxonomyi

Protein namesi
Recommended name:
Toxin zeta
Alternative name(s):
UDP-N-acetylglucosamine kinase (EC:2.7.1.176)
Short name:
UNAG kinase
Encoded oniPlasmid pBT2331 Publication
Plasmid pSM190356 Publications
OrganismiStreptococcus pyogenes
Taxonomic identifieri1314 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi46K → A: Loss of activity. 1 Publication1
Mutagenesisi67D → T: Loss of activity. 1 Publication1
Mutagenesisi158R → A: Loss of activity; when associated with S-171. 1 Publication1
Mutagenesisi171R → S: Loss of activity; when associated with A-158. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002215542 – 287Toxin zetaAdd BLAST286

Interactioni

Subunit structurei

In the presence of the epsilon antitoxin forms an inactive PezA2PezT2 heterotetramer. The heterotetramer is still able to bind the UNAG substrate.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Q572313EBI-6407265,EBI-6407271

Protein-protein interaction databases

DIPiDIP-58969N.
IntActiQ54944. 1 interactor.

Structurei

Secondary structure

1287
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 24Combined sources16
Beta strandi34 – 39Combined sources6
Helixi47 – 56Combined sources10
Turni57 – 59Combined sources3
Beta strandi62 – 64Combined sources3
Helixi66 – 72Combined sources7
Helixi76 – 83Combined sources8
Helixi84 – 86Combined sources3
Helixi88 – 109Combined sources22
Beta strandi113 – 115Combined sources3
Helixi123 – 133Combined sources11
Turni134 – 136Combined sources3
Beta strandi138 – 144Combined sources7
Helixi148 – 165Combined sources18
Turni167 – 169Combined sources3
Helixi175 – 195Combined sources21
Beta strandi201 – 204Combined sources4
Beta strandi210 – 213Combined sources4
Turni214 – 216Combined sources3
Helixi222 – 230Combined sources9
Helixi236 – 252Combined sources17
Helixi259 – 270Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GVNX-ray1.95B/D1-287[»]
3Q8XX-ray2.70B/D1-287[»]
ProteinModelPortaliQ54944.
SMRiQ54944.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ54944.

Family & Domainsi

Sequence similaritiesi

Belongs to the zeta toxin family.Curated

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR010488. Zeta_toxin_domain.
[Graphical view]
PfamiPF06414. Zeta_toxin. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54944-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANIVNFTDK QFENRLNDNL EELIQGKKAV ESPTAFLLGG QPGSGKTSLR
60 70 80 90 100
SAIFEETQGN VIVIDNDTFK QQHPNFDELV KLYEKDVVKH VTPYSNRMTE
110 120 130 140 150
AIISRLSDQG YNLVIEGTGR TTDVPIQTAT MLQAKGYETK MYVMAVPKIN
160 170 180 190 200
SYLGTIERYE TMYADDPMTA RATPKQAHDI VVKNLPTNLE TLHKTGLFSD
210 220 230 240 250
IRLYNREGVK LYSSLETPSI SPKETLEKEL NRKVSGKEIQ PTLERIEQKM
260 270 280
VLNKHQETPE FKAIQQKLES LQPPTPPIPK TPKLPGI
Length:287
Mass (Da):32,404
Last modified:January 23, 2007 - v3
Checksum:iB0C6282EE5B2210F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64695 Genomic DNA. Translation: CAA45934.1.
AY357120 Genomic DNA. Translation: AAR27199.1.
X66468 Genomic DNA. Translation: CAA47091.1.
AY357120 Genomic DNA. Translation: AAR27200.1.
X66468 Genomic DNA. Translation: CAA47092.1.
PIRiS45085.
RefSeqiWP_001284311.1. NC_006979.1.
YP_232738.1. NC_006978.1.
YP_232741.1. NC_006978.1.
YP_232759.1. NC_006979.1.
YP_232762.1. NC_006979.1.

Genome annotation databases

GeneIDi20467746.
20467753.
3355470.
3355471.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64695 Genomic DNA. Translation: CAA45934.1.
AY357120 Genomic DNA. Translation: AAR27199.1.
X66468 Genomic DNA. Translation: CAA47091.1.
AY357120 Genomic DNA. Translation: AAR27200.1.
X66468 Genomic DNA. Translation: CAA47092.1.
PIRiS45085.
RefSeqiWP_001284311.1. NC_006979.1.
YP_232738.1. NC_006978.1.
YP_232741.1. NC_006978.1.
YP_232759.1. NC_006979.1.
YP_232762.1. NC_006979.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GVNX-ray1.95B/D1-287[»]
3Q8XX-ray2.70B/D1-287[»]
ProteinModelPortaliQ54944.
SMRiQ54944.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-58969N.
IntActiQ54944. 1 interactor.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20467746.
20467753.
3355470.
3355471.

Enzyme and pathway databases

BRENDAi2.7.1.176. 5935.

Miscellaneous databases

EvolutionaryTraceiQ54944.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR010488. Zeta_toxin_domain.
[Graphical view]
PfamiPF06414. Zeta_toxin. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiZTOX_STRPY
AccessioniPrimary (citable) accession number: Q54944
Secondary accession number(s): Q54938, Q54939, Q6UZC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 92 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Has a long half-life in vivo.

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.