Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Toxin zeta

Gene
N/A
Organism
Streptococcus pyogenes
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Toxic component of a toxin-antitoxin (TA) module. Phosphorylates UDP-N-acetyl-D-glucosamine (UNAG) on the 3'-hydroxyl group of the N-acetyl-D-glucosamine moiety, yielding UNAG-3P. UNAG-3P inhibits MurA, the first committed step in cell wall synthesis, which is then blocked. Phosphorylation is inhibited by cognate epsilon antitoxin. Part of a postsegregational killing (PSK) system involved in the killing of plasmid-free cells. The zeta toxin induces programmed cell death.1 Publication

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-glucosamine = ADP + UDP-N-acetyl-alpha-D-glucosamine 3'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei66 – 661Substrate
Active sitei67 – 671Proton acceptorCurated
Binding sitei100 – 1001Substrate
Binding sitei118 – 1181Substrate
Binding sitei120 – 1201Substrate
Binding sitei128 – 1281Substrate

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi40 – 478ATPSequence analysis

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Toxin, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.176. 5935.

Names & Taxonomyi

Protein namesi
Recommended name:
Toxin zeta
Alternative name(s):
UDP-N-acetylglucosamine kinase (EC:2.7.1.176)
Short name:
UNAG kinase
Encoded oniPlasmid pBT2331 Publication
Plasmid pSM190356 Publications
OrganismiStreptococcus pyogenes
Taxonomic identifieri1314 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi46 – 461K → A: Loss of activity. 1 Publication
Mutagenesisi67 – 671D → T: Loss of activity. 1 Publication
Mutagenesisi158 – 1581R → A: Loss of activity; when associated with S-171. 1 Publication
Mutagenesisi171 – 1711R → S: Loss of activity; when associated with A-158. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 287286Toxin zetaPRO_0000221554Add
BLAST

Interactioni

Subunit structurei

In the presence of the epsilon antitoxin forms an inactive PezA2PezT2 heterotetramer. The heterotetramer is still able to bind the UNAG substrate.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Q572313EBI-6407265,EBI-6407271

Protein-protein interaction databases

DIPiDIP-58969N.
IntActiQ54944. 1 interaction.

Structurei

Secondary structure

1
287
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 2416Combined sources
Beta strandi34 – 396Combined sources
Helixi47 – 5610Combined sources
Turni57 – 593Combined sources
Beta strandi62 – 643Combined sources
Helixi66 – 727Combined sources
Helixi76 – 838Combined sources
Helixi84 – 863Combined sources
Helixi88 – 10922Combined sources
Beta strandi113 – 1153Combined sources
Helixi123 – 13311Combined sources
Turni134 – 1363Combined sources
Beta strandi138 – 1447Combined sources
Helixi148 – 16518Combined sources
Turni167 – 1693Combined sources
Helixi175 – 19521Combined sources
Beta strandi201 – 2044Combined sources
Beta strandi210 – 2134Combined sources
Turni214 – 2163Combined sources
Helixi222 – 2309Combined sources
Helixi236 – 25217Combined sources
Helixi259 – 27012Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GVNX-ray1.95B/D1-287[»]
3Q8XX-ray2.70B/D1-287[»]
ProteinModelPortaliQ54944.
SMRiQ54944. Positions 2-273.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ54944.

Family & Domainsi

Sequence similaritiesi

Belongs to the zeta toxin family.Curated

Phylogenomic databases

KOiK16214.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR010488. Zeta_toxin_domain.
[Graphical view]
PfamiPF06414. Zeta_toxin. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54944-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANIVNFTDK QFENRLNDNL EELIQGKKAV ESPTAFLLGG QPGSGKTSLR
60 70 80 90 100
SAIFEETQGN VIVIDNDTFK QQHPNFDELV KLYEKDVVKH VTPYSNRMTE
110 120 130 140 150
AIISRLSDQG YNLVIEGTGR TTDVPIQTAT MLQAKGYETK MYVMAVPKIN
160 170 180 190 200
SYLGTIERYE TMYADDPMTA RATPKQAHDI VVKNLPTNLE TLHKTGLFSD
210 220 230 240 250
IRLYNREGVK LYSSLETPSI SPKETLEKEL NRKVSGKEIQ PTLERIEQKM
260 270 280
VLNKHQETPE FKAIQQKLES LQPPTPPIPK TPKLPGI
Length:287
Mass (Da):32,404
Last modified:January 23, 2007 - v3
Checksum:iB0C6282EE5B2210F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64695 Genomic DNA. Translation: CAA45934.1.
AY357120 Genomic DNA. Translation: AAR27199.1.
X66468 Genomic DNA. Translation: CAA47091.1.
AY357120 Genomic DNA. Translation: AAR27200.1.
X66468 Genomic DNA. Translation: CAA47092.1.
PIRiS45085.
RefSeqiWP_001284311.1. NC_006979.1.
YP_232738.1. NC_006978.1.
YP_232741.1. NC_006978.1.
YP_232759.1. NC_006979.1.
YP_232762.1. NC_006979.1.

Genome annotation databases

GeneIDi20467746.
20467753.
3355470.
3355471.
KEGGipg:20467746.
pg:20467753.
pg:3355470.
pg:3355471.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64695 Genomic DNA. Translation: CAA45934.1.
AY357120 Genomic DNA. Translation: AAR27199.1.
X66468 Genomic DNA. Translation: CAA47091.1.
AY357120 Genomic DNA. Translation: AAR27200.1.
X66468 Genomic DNA. Translation: CAA47092.1.
PIRiS45085.
RefSeqiWP_001284311.1. NC_006979.1.
YP_232738.1. NC_006978.1.
YP_232741.1. NC_006978.1.
YP_232759.1. NC_006979.1.
YP_232762.1. NC_006979.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GVNX-ray1.95B/D1-287[»]
3Q8XX-ray2.70B/D1-287[»]
ProteinModelPortaliQ54944.
SMRiQ54944. Positions 2-273.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-58969N.
IntActiQ54944. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20467746.
20467753.
3355470.
3355471.
KEGGipg:20467746.
pg:20467753.
pg:3355470.
pg:3355471.

Phylogenomic databases

KOiK16214.

Enzyme and pathway databases

BRENDAi2.7.1.176. 5935.

Miscellaneous databases

EvolutionaryTraceiQ54944.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR010488. Zeta_toxin_domain.
[Graphical view]
PfamiPF06414. Zeta_toxin. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiZTOX_STRPY
AccessioniPrimary (citable) accession number: Q54944
Secondary accession number(s): Q54938, Q54939, Q6UZC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 89 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Has a long half-life in vivo.

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.