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Q54801 (DYR_STRPN) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrofolate reductase

EC=1.5.1.3
Gene names
Name:dhfR
Synonyms:dfr
Ordered Locus Names:SP_1571
OrganismStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) [Complete proteome] [HAMAP]
Taxonomic identifier170187 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length168 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Miscellaneous

Strains 1/1, 21, 30, 56 and 92 are trimethoprim resistant.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SP_0371Q97SJ04EBI-6473264,EBI-6473258

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 168168Dihydrofolate reductase
PRO_0000186417

Regions

Domain4 – 165162DHFR
Nucleotide binding9 – 102NADP By similarity
Nucleotide binding17 – 226NADP By similarity
Nucleotide binding46 – 494NADP By similarity
Nucleotide binding66 – 694NADP By similarity
Nucleotide binding100 – 1056NADP By similarity
Region8 – 103Substrate binding By similarity

Sites

Binding site301Substrate By similarity
Binding site611Substrate By similarity
Binding site831NADP; via amide nitrogen By similarity
Binding site1191Substrate By similarity

Natural variations

Natural variant141E → D in strain: 21.
Natural variant201E → D in strain: 21, 30 and 1/1.
Natural variant601K → Q in strain: 30, 56 and 1/1.
Natural variant651I → V in strain: 92.
Natural variant701P → S in strain: 21, 56 and 1/1.
Natural variant741I → L in strain: 21.
Natural variant77 – 782VA → AV in strain: 30.
Natural variant811Q → H in strain: 21, 56 and 1/1.
Natural variant911Q → H in strain: 21.
Natural variant921A → D in strain: ATCC 49619.
Natural variant941E → D in strain: 21.
Natural variant1001I → L in strain: 21, 30, 56 and 1/1.
Natural variant1111P → S in strain: 56 and 1/1.
Natural variant1351L → F in strain: 21, 30, 56 and 1/1.
Natural variant1411E → D in strain: 56.
Natural variant147 – 1493FYA → SYT in strain: 21.

Secondary structure

.............................. 168
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q54801 [UniParc].

Last modified September 26, 2001. Version 2.
Checksum: 4C3887E290B6303E

FASTA16819,703
        10         20         30         40         50         60 
MTKKIVAIWA QDEEGVIGKE NRLPWHLPAE LQHFKETTLN HAILMGRVTF DGMGRRLLPK 

        70         80         90        100        110        120 
RETLILTRNP EEKIDGVATF QDVQSVLDWY QAQEKNLYII GGKQIFQAFE PYLDEVIVTH 

       130        140        150        160 
IHARVEGDTY FPEELDLSLF ETVSSKFYAK DEKNPYDFTI QYRKRKEV 

« Hide

References

« Hide 'large scale' references
[1]Caspers P.
Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 49619 / 262 / CIP 104340 / Serotype 19F and Trimethoprim resistant 1/1 Johannesburg 1993.
[2]"Mutations in the dihydrofolate reductase gene of trimethoprim-resistant isolates of Streptococcus pneumoniae."
Adrian P.V., Klugman K.P.
Antimicrob. Agents Chemother. 41:2406-2413(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 30, 56, 92 and Trimethoprim resistant 21.
[3]"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae."
Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. expand/collapse author list , Lewis M.R., Radune D., Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M.
Science 293:498-506(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-334 / TIGR4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z74777 Genomic DNA. Translation: CAA99035.1.
Z74778 Genomic DNA. Translation: CAA99036.1.
Z84378 Genomic DNA. Translation: CAB06418.1.
Z84379 Genomic DNA. Translation: CAB06419.1.
Z84380 Genomic DNA. Translation: CAB06420.1.
Z84381 Genomic DNA. Translation: CAB06421.1.
AE005672 Genomic DNA. Translation: AAK75658.1.
PIRA95183.
RefSeqNP_346018.1. NC_003028.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3IX9X-ray1.95A/B1-168[»]
ProteinModelPortalQ54801.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ54801. 1 interaction.
STRING170187.SP_1571.

Chemistry

BindingDBQ54801.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK75658; AAK75658; SP_1571.
GeneID931270.
KEGGspn:SP_1571.
PATRIC19707567. VBIStrPne105772_1626.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0262.
HOGENOMHOG000040233.
KOK00287.
OMADTQFPDW.
OrthoDBEOG6KT2V2.
ProtClustDBCLSK884121.

Enzyme and pathway databases

BioCycSPNE170187:GHGN-1577-MONOMER.
UniPathwayUPA00077; UER00158.

Family and domain databases

Gene3D3.40.430.10. 1 hit.
InterProIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFPIRSF000194. DHFR. 1 hit.
PRINTSPR00070. DHFR.
SUPFAMSSF53597. SSF53597. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ54801.

Entry information

Entry nameDYR_STRPN
AccessionPrimary (citable) accession number: Q54801
Secondary accession number(s): O08440 expand/collapse secondary AC list , O08441, O08442, O08443, Q54973
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 26, 2001
Last modified: December 11, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways