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Q54801

- DYR_STRPN

UniProt

Q54801 - DYR_STRPN

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Protein

Dihydrofolate reductase

Gene

dhfR

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei30 – 301SubstrateBy similarity
Binding sitei61 – 611SubstrateBy similarity
Binding sitei83 – 831NADP; via amide nitrogenBy similarity
Binding sitei119 – 1191SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 102NADPBy similarity
Nucleotide bindingi17 – 226NADPBy similarity
Nucleotide bindingi46 – 494NADPBy similarity
Nucleotide bindingi66 – 694NADPBy similarity
Nucleotide bindingi100 – 1056NADPBy similarity

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. NADP binding Source: InterPro

GO - Biological processi

  1. glycine biosynthetic process Source: InterPro
  2. nucleotide biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. response to antibiotic Source: UniProtKB-KW
  5. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic resistance, One-carbon metabolism, Trimethoprim resistance

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciSPNE170187:GHGN-1577-MONOMER.
UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:dhfR
Synonyms:dfr
Ordered Locus Names:SP_1571
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000585: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 168168Dihydrofolate reductasePRO_0000186417Add
BLAST

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SP_0371Q97SJ04EBI-6473264,EBI-6473258

Protein-protein interaction databases

IntActiQ54801. 1 interaction.
STRINGi170187.SP_1571.

Structurei

Secondary structure

1
168
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 117
Beta strandi16 – 194
Helixi28 – 3811
Beta strandi41 – 466
Helixi47 – 526
Turni53 – 553
Beta strandi62 – 665
Beta strandi75 – 828
Helixi83 – 919
Beta strandi97 – 1015
Helixi103 – 1097
Helixi110 – 1123
Beta strandi114 – 12310
Beta strandi128 – 1303
Helixi137 – 1393
Beta strandi140 – 1489
Beta strandi158 – 1658

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3IX9X-ray1.95A/B1-168[»]
ProteinModelPortaliQ54801.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ54801.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 165162DHFRPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni8 – 103Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated
Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0262.
HOGENOMiHOG000040233.
KOiK00287.
OMAiFKALTMG.
OrthoDBiEOG6KT2V2.

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000194. DHFR. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54801-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTKKIVAIWA QDEEGVIGKE NRLPWHLPAE LQHFKETTLN HAILMGRVTF
60 70 80 90 100
DGMGRRLLPK RETLILTRNP EEKIDGVATF QDVQSVLDWY QAQEKNLYII
110 120 130 140 150
GGKQIFQAFE PYLDEVIVTH IHARVEGDTY FPEELDLSLF ETVSSKFYAK
160
DEKNPYDFTI QYRKRKEV
Length:168
Mass (Da):19,703
Last modified:September 26, 2001 - v2
Checksum:i4C3887E290B6303E
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti14 – 141E → D in strain: 21.
Natural varianti20 – 201E → D in strain: 21, 30 and 1/1.
Natural varianti60 – 601K → Q in strain: 30, 56 and 1/1.
Natural varianti65 – 651I → V in strain: 92.
Natural varianti70 – 701P → S in strain: 21, 56 and 1/1.
Natural varianti74 – 741I → L in strain: 21.
Natural varianti77 – 782VA → AV in strain: 30.
Natural varianti81 – 811Q → H in strain: 21, 56 and 1/1.
Natural varianti91 – 911Q → H in strain: 21.
Natural varianti92 – 921A → D in strain: ATCC 49619.
Natural varianti94 – 941E → D in strain: 21.
Natural varianti100 – 1001I → L in strain: 21, 30, 56 and 1/1.
Natural varianti111 – 1111P → S in strain: 56 and 1/1.
Natural varianti135 – 1351L → F in strain: 21, 30, 56 and 1/1.
Natural varianti141 – 1411E → D in strain: 56.
Natural varianti147 – 1493FYA → SYT in strain: 21.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z74777 Genomic DNA. Translation: CAA99035.1.
Z74778 Genomic DNA. Translation: CAA99036.1.
Z84378 Genomic DNA. Translation: CAB06418.1.
Z84379 Genomic DNA. Translation: CAB06419.1.
Z84380 Genomic DNA. Translation: CAB06420.1.
Z84381 Genomic DNA. Translation: CAB06421.1.
AE005672 Genomic DNA. Translation: AAK75658.1.
PIRiA95183.
RefSeqiNP_346018.1. NC_003028.3.

Genome annotation databases

EnsemblBacteriaiAAK75658; AAK75658; SP_1571.
GeneIDi931270.
KEGGispn:SP_1571.
PATRICi19707567. VBIStrPne105772_1626.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z74777 Genomic DNA. Translation: CAA99035.1 .
Z74778 Genomic DNA. Translation: CAA99036.1 .
Z84378 Genomic DNA. Translation: CAB06418.1 .
Z84379 Genomic DNA. Translation: CAB06419.1 .
Z84380 Genomic DNA. Translation: CAB06420.1 .
Z84381 Genomic DNA. Translation: CAB06421.1 .
AE005672 Genomic DNA. Translation: AAK75658.1 .
PIRi A95183.
RefSeqi NP_346018.1. NC_003028.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3IX9 X-ray 1.95 A/B 1-168 [» ]
ProteinModelPortali Q54801.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q54801. 1 interaction.
STRINGi 170187.SP_1571.

Chemistry

BindingDBi Q54801.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK75658 ; AAK75658 ; SP_1571 .
GeneIDi 931270.
KEGGi spn:SP_1571.
PATRICi 19707567. VBIStrPne105772_1626.

Phylogenomic databases

eggNOGi COG0262.
HOGENOMi HOG000040233.
KOi K00287.
OMAi FKALTMG.
OrthoDBi EOG6KT2V2.

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00158 .
BioCyci SPNE170187:GHGN-1577-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q54801.

Family and domain databases

Gene3Di 3.40.430.10. 1 hit.
InterProi IPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view ]
Pfami PF00186. DHFR_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000194. DHFR. 1 hit.
PRINTSi PR00070. DHFR.
SUPFAMi SSF53597. SSF53597. 1 hit.
PROSITEi PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Caspers P.
    Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 49619 / 262 / CIP 104340 / Serotype 19F and Trimethoprim resistant 1/1 Johannesburg 1993.
  2. "Mutations in the dihydrofolate reductase gene of trimethoprim-resistant isolates of Streptococcus pneumoniae."
    Adrian P.V., Klugman K.P.
    Antimicrob. Agents Chemother. 41:2406-2413(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 30, 56, 92 and Trimethoprim resistant 21.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-334 / TIGR4.

Entry informationi

Entry nameiDYR_STRPN
AccessioniPrimary (citable) accession number: Q54801
Secondary accession number(s): O08440
, O08441, O08442, O08443, Q54973
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 26, 2001
Last modified: October 29, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Strains 1/1, 21, 30, 56 and 92 are trimethoprim resistant.

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3