ID NANB_STRPN Reviewed; 697 AA. AC Q54727; DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot. DT 26-SEP-2001, sequence version 2. DT 27-MAR-2024, entry version 159. DE RecName: Full=Sialidase B; DE EC=3.2.1.18; DE AltName: Full=Neuraminidase B; DE Flags: Precursor; GN Name=nanB; OrderedLocusNames=SP_1687; OS Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4). OC Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae; OC Streptococcus. OX NCBI_TaxID=170187; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=Serotype 6; RX PubMed=8759848; DOI=10.1128/jb.178.16.4854-4860.1996; RA Berry A.M., Lock R.A., Paton J.C.; RT "Cloning and characterization of nanB, a second Streptococcus pneumoniae RT neuraminidase gene, and purification of the NanB enzyme from recombinant RT Escherichia coli."; RL J. Bacteriol. 178:4854-4860(1996). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC BAA-334 / TIGR4; RX PubMed=11463916; DOI=10.1126/science.1061217; RA Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., RA Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., RA Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., RA Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D., RA Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., RA McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., RA Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., RA Morrison D.A., Hollingshead S.K., Fraser C.M.; RT "Complete genome sequence of a virulent isolate of Streptococcus RT pneumoniae."; RL Science 293:498-506(2001). CC -!- CATALYTIC ACTIVITY: CC Reaction=Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha- CC (2->8)- glycosidic linkages of terminal sialic acid residues in CC oligosaccharides, glycoproteins, glycolipids, colominic acid and CC synthetic substrates.; EC=3.2.1.18; CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 33 family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U43526; AAC44396.1; -; Genomic_DNA. DR EMBL; AE005672; AAK75766.1; -; Genomic_DNA. DR PIR; E95196; E95196. DR RefSeq; WP_001042179.1; NZ_CP089948.1. DR PDB; 2JKB; X-ray; 1.54 A; A=30-697. DR PDB; 2VW0; X-ray; 2.30 A; A=1-697. DR PDB; 2VW1; X-ray; 2.39 A; A=1-697. DR PDB; 2VW2; X-ray; 1.70 A; A=1-697. DR PDB; 4FOQ; X-ray; 1.99 A; A=1-697. DR PDB; 4FOV; X-ray; 2.29 A; A=1-697. DR PDB; 4FOW; X-ray; 2.10 A; A=1-697. DR PDB; 4FOY; X-ray; 1.84 A; A=1-697. DR PDB; 4FP2; X-ray; 2.05 A; A=1-697. DR PDB; 4FP3; X-ray; 2.74 A; A=1-697. DR PDB; 4FPC; X-ray; 2.32 A; A=1-697. DR PDB; 4FPE; X-ray; 2.18 A; A=1-697. DR PDB; 4FPF; X-ray; 2.23 A; A=1-697. DR PDB; 4FPG; X-ray; 2.58 A; A=1-697. DR PDB; 4FPH; X-ray; 1.94 A; A=1-697. DR PDB; 4FPJ; X-ray; 1.98 A; A=1-697. DR PDB; 4FPK; X-ray; 2.40 A; A=1-697. DR PDB; 4FPL; X-ray; 2.10 A; A=1-697. DR PDB; 4FPO; X-ray; 2.59 A; B=1-697. DR PDB; 4FPY; X-ray; 2.18 A; A=1-697. DR PDB; 4FQ4; X-ray; 1.84 A; A=1-697. DR PDB; 4XE9; X-ray; 1.84 A; A=39-696. DR PDB; 4XHB; X-ray; 1.88 A; A=39-696. DR PDB; 4XHX; X-ray; 2.10 A; A=39-696. DR PDB; 4XIK; X-ray; 1.91 A; A=39-696. DR PDB; 4XIL; X-ray; 1.90 A; A=39-696. DR PDB; 4XIO; X-ray; 1.80 A; A=39-696. DR PDB; 4XJ8; X-ray; 2.75 A; A=39-696. DR PDB; 4XJ9; X-ray; 1.98 A; A=39-696. DR PDB; 4XJA; X-ray; 1.98 A; A=39-696. DR PDB; 4XJU; X-ray; 1.94 A; A=39-696. DR PDB; 4XJW; X-ray; 1.53 A; A=39-696. DR PDB; 4XJZ; X-ray; 1.56 A; A=39-696. DR PDB; 4XMA; X-ray; 2.09 A; A=39-696. DR PDB; 4XMI; X-ray; 1.97 A; A=39-696. DR PDB; 4XOG; X-ray; 2.09 A; A=37-694. DR PDB; 4XYX; X-ray; 2.10 A; A=40-697. DR PDBsum; 2JKB; -. DR PDBsum; 2VW0; -. DR PDBsum; 2VW1; -. DR PDBsum; 2VW2; -. DR PDBsum; 4FOQ; -. DR PDBsum; 4FOV; -. DR PDBsum; 4FOW; -. DR PDBsum; 4FOY; -. DR PDBsum; 4FP2; -. DR PDBsum; 4FP3; -. DR PDBsum; 4FPC; -. DR PDBsum; 4FPE; -. DR PDBsum; 4FPF; -. DR PDBsum; 4FPG; -. DR PDBsum; 4FPH; -. DR PDBsum; 4FPJ; -. DR PDBsum; 4FPK; -. DR PDBsum; 4FPL; -. DR PDBsum; 4FPO; -. DR PDBsum; 4FPY; -. DR PDBsum; 4FQ4; -. DR PDBsum; 4XE9; -. DR PDBsum; 4XHB; -. DR PDBsum; 4XHX; -. DR PDBsum; 4XIK; -. DR PDBsum; 4XIL; -. DR PDBsum; 4XIO; -. DR PDBsum; 4XJ8; -. DR PDBsum; 4XJ9; -. DR PDBsum; 4XJA; -. DR PDBsum; 4XJU; -. DR PDBsum; 4XJW; -. DR PDBsum; 4XJZ; -. DR PDBsum; 4XMA; -. DR PDBsum; 4XMI; -. DR PDBsum; 4XOG; -. DR PDBsum; 4XYX; -. DR AlphaFoldDB; Q54727; -. DR SMR; Q54727; -. DR ChEMBL; CHEMBL4295604; -. DR CAZy; CBM40; Carbohydrate-Binding Module Family 40. DR CAZy; GH33; Glycoside Hydrolase Family 33. DR PaxDb; 170187-SP_1687; -. DR EnsemblBacteria; AAK75766; AAK75766; SP_1687. DR KEGG; spn:SP_1687; -. DR eggNOG; COG4409; Bacteria. DR BioCyc; SPNE170187:G1FZB-1708-MONOMER; -. DR BRENDA; 3.2.1.18; 1960. DR SABIO-RK; Q54727; -. DR EvolutionaryTrace; Q54727; -. DR PRO; PR:Q54727; -. DR Proteomes; UP000000585; Chromosome. DR GO; GO:0052794; F:exo-alpha-(2->3)-sialidase activity; IEA:UniProtKB-EC. DR GO; GO:0052795; F:exo-alpha-(2->6)-sialidase activity; IEA:UniProtKB-EC. DR GO; GO:0052796; F:exo-alpha-(2->8)-sialidase activity; IEA:UniProtKB-EC. DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro. DR CDD; cd15482; Sialidase_non-viral; 1. DR Gene3D; 2.120.10.10; -; 1. DR Gene3D; 2.60.120.200; -; 1. DR InterPro; IPR002860; BNR_rpt. DR InterPro; IPR013320; ConA-like_dom_sf. DR InterPro; IPR004124; Glyco_hydro_33_N. DR InterPro; IPR011040; Sialidase. DR InterPro; IPR026856; Sialidase_fam. DR InterPro; IPR036278; Sialidase_sf. DR InterPro; IPR023364; Trans_sialidase_dom3. DR PANTHER; PTHR10628; SIALIDASE; 1. DR PANTHER; PTHR10628:SF19; SIALIDASE; 1. DR Pfam; PF02012; BNR; 1. DR Pfam; PF13088; BNR_2; 1. DR Pfam; PF02973; Sialidase; 1. DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1. DR SUPFAM; SSF50939; Sialidases; 1. PE 1: Evidence at protein level; KW 3D-structure; Glycosidase; Hydrolase; Reference proteome; Repeat; Signal. FT SIGNAL 1..29 FT CHAIN 30..697 FT /note="Sialidase B" FT /id="PRO_0000012038" FT REPEAT 280..291 FT /note="BNR 1" FT REPEAT 462..473 FT /note="BNR 2" FT REPEAT 517..528 FT /note="BNR 3" FT REPEAT 566..577 FT /note="BNR 4" FT ACT_SITE 270 FT /note="Proton acceptor" FT /evidence="ECO:0000250" FT ACT_SITE 541 FT /evidence="ECO:0000255" FT ACT_SITE 653 FT /note="Nucleophile" FT /evidence="ECO:0000250" FT BINDING 245 FT /ligand="substrate" FT /evidence="ECO:0000250" FT BINDING 557 FT /ligand="substrate" FT /evidence="ECO:0000250" FT BINDING 619 FT /ligand="substrate" FT /evidence="ECO:0000250" FT CONFLICT 121 FT /note="K -> E (in Ref. 1; AAC44396)" FT /evidence="ECO:0000305" FT CONFLICT 206 FT /note="D -> G (in Ref. 1; AAC44396)" FT /evidence="ECO:0000305" FT CONFLICT 222 FT /note="T -> N (in Ref. 1; AAC44396)" FT /evidence="ECO:0000305" FT CONFLICT 393 FT /note="N -> D (in Ref. 1; AAC44396)" FT /evidence="ECO:0000305" FT CONFLICT 639 FT /note="K -> R (in Ref. 1; AAC44396)" FT /evidence="ECO:0000305" FT STRAND 42..53 FT /evidence="ECO:0007829|PDB:4XJW" FT HELIX 58..61 FT /evidence="ECO:0007829|PDB:4XJW" FT TURN 62..64 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 70..77 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 83..91 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 101..107 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 112..118 FT /evidence="ECO:0007829|PDB:4XJW" FT TURN 119..122 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 123..131 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 136..141 FT /evidence="ECO:0007829|PDB:2VW1" FT STRAND 144..151 FT /evidence="ECO:0007829|PDB:4XJW" FT TURN 152..155 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 156..161 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 164..170 FT /evidence="ECO:0007829|PDB:4XJW" FT HELIX 177..179 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 185..193 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 196..199 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 202..213 FT /evidence="ECO:0007829|PDB:4XJW" FT HELIX 217..220 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 242..245 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 257..266 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 271..273 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 275..284 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 293..296 FT /evidence="ECO:0007829|PDB:4XJW" FT TURN 312..316 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 324..332 FT /evidence="ECO:0007829|PDB:4XJW" FT TURN 334..336 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 339..346 FT /evidence="ECO:0007829|PDB:4XJW" FT TURN 352..354 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 361..365 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 368..375 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 383..385 FT /evidence="ECO:0007829|PDB:4XJW" FT HELIX 387..389 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 390..393 FT /evidence="ECO:0007829|PDB:4XJW" FT TURN 394..397 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 398..404 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 408..412 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 418..425 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 432..441 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 446..448 FT /evidence="ECO:0007829|PDB:4FPG" FT STRAND 450..452 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 458..466 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 467..469 FT /evidence="ECO:0007829|PDB:4FP3" FT STRAND 483..485 FT /evidence="ECO:0007829|PDB:4FPO" FT STRAND 492..494 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 499..502 FT /evidence="ECO:0007829|PDB:4XIL" FT STRAND 504..510 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 513..521 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 527..532 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 542..548 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 551..556 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 559..562 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 564..570 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 573..575 FT /evidence="ECO:0007829|PDB:4FP3" FT STRAND 594..597 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 607..613 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 616..620 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 622..629 FT /evidence="ECO:0007829|PDB:4XJW" FT TURN 631..633 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 636..645 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 653..658 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 664..669 FT /evidence="ECO:0007829|PDB:4XJW" FT STRAND 676..678 FT /evidence="ECO:0007829|PDB:2VW1" FT STRAND 685..690 FT /evidence="ECO:0007829|PDB:4XJW" FT HELIX 692..695 FT /evidence="ECO:0007829|PDB:4XJW" SQ SEQUENCE 697 AA; 77695 MW; A0D9F9AFDE46AFD7 CRC64; MNKRGLYSKL GISVVGISLL MGVPTLIHAN ELNYGQLSIS PIFQGGSYQL NNKSIDISSL LLDKLSGESQ TVVMKFKADK PNSLQALFGL SNSKAGFKNN YFSIFMRDSG EIGVEIRDAQ KGINYLFSRP ASLWGKHKGQ AVENTLVFVS DSKDKTYTMY VNGIEVFSET VDTFLPISNI NGIDKATLGA VNREGKEHYL AKGSIDEISL FNKAISDQEV STIPLSNPFQ LIFQSGDSTQ ANYFRIPTLY TLSSGRVLSS IDARYGGTHD SKSKINIATS YSDDNGKTWS EPIFAMKFND YEEQLVYWPR DNKLKNSQIS GSASFIDSSI VEDKKSGKTI LLADVMPAGI GNNNANKADS GFKEINGHYY LKLKKNGDND FRYTVRENGV VYNETTNKPT NYTINDKYEV LEGGKSLTVE QYSVDFDSGS LRERHNGKQV PMNVFYKDSL FKVTPTNYIA MTTSQNRGES WEQFKLLPPF LGEKHNGTYL CPGQGLALKS SNRLIFATYT SGELTYLISD DSGQTWKKSS ASIPFKNATA EAQMVELRDG VIRTFFRTTT GKIAYMTSRD SGETWSKVSY IDGIQQTSYG TQVSAIKYSQ LIDGKEAVIL STPNSRSGRK GGQLVVGLVN KEDDSIDWKY HYDIDLPSYG YAYSAITELP NHHIGVLFEK YDSWSRNELH LSNVVQYIDL EINDLTK //