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Q54727

- NANB_STRPN

UniProt

Q54727 - NANB_STRPN

Protein

Sialidase B

Gene

nanB

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 2 (26 Sep 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei245 – 2451SubstrateBy similarity
    Active sitei270 – 2701Proton acceptorBy similarity
    Active sitei541 – 5411Sequence Analysis
    Binding sitei557 – 5571SubstrateBy similarity
    Binding sitei619 – 6191SubstrateBy similarity
    Active sitei653 – 6531NucleophileBy similarity

    GO - Molecular functioni

    1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
    2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
    3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciSPNE170187:GHGN-1694-MONOMER.
    BRENDAi3.2.1.18. 5946.
    SABIO-RKQ54727.

    Protein family/group databases

    CAZyiCBM40. Carbohydrate-Binding Module Family 40.
    GH33. Glycoside Hydrolase Family 33.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sialidase B (EC:3.2.1.18)
    Alternative name(s):
    Neuraminidase B
    Gene namesi
    Name:nanB
    Ordered Locus Names:SP_1687
    OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
    Taxonomic identifieri170187 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000000585: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Add
    BLAST
    Chaini30 – 697668Sialidase BPRO_0000012038Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi170187.SP_1687.

    Structurei

    Secondary structure

    1
    697
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi42 – 5312
    Helixi58 – 614
    Helixi62 – 643
    Beta strandi70 – 778
    Beta strandi83 – 919
    Beta strandi101 – 1077
    Beta strandi112 – 1187
    Turni119 – 1224
    Beta strandi123 – 1319
    Beta strandi136 – 1416
    Beta strandi144 – 1518
    Turni152 – 1554
    Beta strandi156 – 1616
    Beta strandi164 – 1707
    Helixi177 – 1793
    Beta strandi185 – 1939
    Beta strandi196 – 1994
    Beta strandi202 – 21312
    Helixi217 – 2215
    Beta strandi242 – 2454
    Beta strandi257 – 26610
    Beta strandi271 – 2733
    Beta strandi275 – 28410
    Beta strandi293 – 2964
    Turni312 – 3165
    Beta strandi324 – 3329
    Turni334 – 3363
    Beta strandi339 – 3468
    Turni352 – 3543
    Beta strandi361 – 37515
    Beta strandi383 – 3853
    Helixi387 – 3893
    Beta strandi390 – 3934
    Turni394 – 3974
    Beta strandi398 – 4047
    Beta strandi408 – 4125
    Beta strandi418 – 4258
    Beta strandi432 – 44110
    Beta strandi446 – 4483
    Beta strandi450 – 4523
    Beta strandi458 – 4669
    Beta strandi467 – 4693
    Beta strandi483 – 4853
    Beta strandi492 – 4943
    Beta strandi499 – 5024
    Beta strandi504 – 5107
    Beta strandi513 – 5219
    Beta strandi527 – 5326
    Beta strandi542 – 5487
    Beta strandi551 – 5566
    Beta strandi559 – 5624
    Beta strandi564 – 5707
    Beta strandi573 – 5753
    Beta strandi594 – 6029
    Beta strandi605 – 6139
    Beta strandi616 – 6205
    Beta strandi622 – 6298
    Turni631 – 6333
    Beta strandi636 – 64510
    Beta strandi653 – 6586
    Beta strandi664 – 6696
    Beta strandi676 – 6783
    Beta strandi685 – 6906
    Helixi692 – 6954

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2JKBX-ray1.54A30-697[»]
    2VW0X-ray2.30A1-697[»]
    2VW1X-ray2.39A1-697[»]
    2VW2X-ray1.70A1-697[»]
    4FOQX-ray1.99A1-697[»]
    4FOVX-ray2.29A1-697[»]
    4FOWX-ray2.10A1-697[»]
    4FOYX-ray1.84A1-697[»]
    4FP2X-ray2.05A1-697[»]
    4FP3X-ray2.74A1-697[»]
    4FPCX-ray2.32A1-697[»]
    4FPEX-ray2.18A1-697[»]
    4FPFX-ray2.23A1-697[»]
    4FPGX-ray2.58A1-697[»]
    4FPHX-ray1.94A1-697[»]
    4FPJX-ray1.98A1-697[»]
    4FPKX-ray2.40A1-697[»]
    4FPLX-ray2.10A1-697[»]
    4FPOX-ray2.59B1-697[»]
    4FPYX-ray2.18A1-697[»]
    4FQ4X-ray1.84A1-697[»]
    ProteinModelPortaliQ54727.
    SMRiQ54727. Positions 39-696.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ54727.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati280 – 29112BNR 1Add
    BLAST
    Repeati462 – 47312BNR 2Add
    BLAST
    Repeati517 – 52812BNR 3Add
    BLAST
    Repeati566 – 57712BNR 4Add
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 33 family.Curated
    Contains 4 BNR repeats.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG4409.
    HOGENOMiHOG000285031.
    KOiK01186.
    OMAiWRYHYDI.
    OrthoDBiEOG61CM2G.

    Family and domain databases

    Gene3Di2.120.10.10. 2 hits.
    2.40.220.10. 1 hit.
    2.60.120.200. 1 hit.
    InterProiIPR002860. BNR_rpt.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR004124. Glyco_hydro_33_N.
    IPR011040. Sialidases.
    IPR023364. Trans_sialidase_dom3.
    [Graphical view]
    PfamiPF02012. BNR. 1 hit.
    PF02973. Sialidase. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 1 hit.
    SSF50939. SSF50939. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q54727-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNKRGLYSKL GISVVGISLL MGVPTLIHAN ELNYGQLSIS PIFQGGSYQL    50
    NNKSIDISSL LLDKLSGESQ TVVMKFKADK PNSLQALFGL SNSKAGFKNN 100
    YFSIFMRDSG EIGVEIRDAQ KGINYLFSRP ASLWGKHKGQ AVENTLVFVS 150
    DSKDKTYTMY VNGIEVFSET VDTFLPISNI NGIDKATLGA VNREGKEHYL 200
    AKGSIDEISL FNKAISDQEV STIPLSNPFQ LIFQSGDSTQ ANYFRIPTLY 250
    TLSSGRVLSS IDARYGGTHD SKSKINIATS YSDDNGKTWS EPIFAMKFND 300
    YEEQLVYWPR DNKLKNSQIS GSASFIDSSI VEDKKSGKTI LLADVMPAGI 350
    GNNNANKADS GFKEINGHYY LKLKKNGDND FRYTVRENGV VYNETTNKPT 400
    NYTINDKYEV LEGGKSLTVE QYSVDFDSGS LRERHNGKQV PMNVFYKDSL 450
    FKVTPTNYIA MTTSQNRGES WEQFKLLPPF LGEKHNGTYL CPGQGLALKS 500
    SNRLIFATYT SGELTYLISD DSGQTWKKSS ASIPFKNATA EAQMVELRDG 550
    VIRTFFRTTT GKIAYMTSRD SGETWSKVSY IDGIQQTSYG TQVSAIKYSQ 600
    LIDGKEAVIL STPNSRSGRK GGQLVVGLVN KEDDSIDWKY HYDIDLPSYG 650
    YAYSAITELP NHHIGVLFEK YDSWSRNELH LSNVVQYIDL EINDLTK 697
    Length:697
    Mass (Da):77,695
    Last modified:September 26, 2001 - v2
    Checksum:iA0D9F9AFDE46AFD7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti121 – 1211K → E in AAC44396. (PubMed:8759848)Curated
    Sequence conflicti206 – 2061D → G in AAC44396. (PubMed:8759848)Curated
    Sequence conflicti222 – 2221T → N in AAC44396. (PubMed:8759848)Curated
    Sequence conflicti393 – 3931N → D in AAC44396. (PubMed:8759848)Curated
    Sequence conflicti639 – 6391K → R in AAC44396. (PubMed:8759848)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43526 Genomic DNA. Translation: AAC44396.1.
    AE005672 Genomic DNA. Translation: AAK75766.1.
    PIRiE95196.
    RefSeqiNP_346126.1. NC_003028.3.

    Genome annotation databases

    EnsemblBacteriaiAAK75766; AAK75766; SP_1687.
    GeneIDi931147.
    KEGGispn:SP_1687.
    PATRICi19707809. VBIStrPne105772_1747.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43526 Genomic DNA. Translation: AAC44396.1 .
    AE005672 Genomic DNA. Translation: AAK75766.1 .
    PIRi E95196.
    RefSeqi NP_346126.1. NC_003028.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2JKB X-ray 1.54 A 30-697 [» ]
    2VW0 X-ray 2.30 A 1-697 [» ]
    2VW1 X-ray 2.39 A 1-697 [» ]
    2VW2 X-ray 1.70 A 1-697 [» ]
    4FOQ X-ray 1.99 A 1-697 [» ]
    4FOV X-ray 2.29 A 1-697 [» ]
    4FOW X-ray 2.10 A 1-697 [» ]
    4FOY X-ray 1.84 A 1-697 [» ]
    4FP2 X-ray 2.05 A 1-697 [» ]
    4FP3 X-ray 2.74 A 1-697 [» ]
    4FPC X-ray 2.32 A 1-697 [» ]
    4FPE X-ray 2.18 A 1-697 [» ]
    4FPF X-ray 2.23 A 1-697 [» ]
    4FPG X-ray 2.58 A 1-697 [» ]
    4FPH X-ray 1.94 A 1-697 [» ]
    4FPJ X-ray 1.98 A 1-697 [» ]
    4FPK X-ray 2.40 A 1-697 [» ]
    4FPL X-ray 2.10 A 1-697 [» ]
    4FPO X-ray 2.59 B 1-697 [» ]
    4FPY X-ray 2.18 A 1-697 [» ]
    4FQ4 X-ray 1.84 A 1-697 [» ]
    ProteinModelPortali Q54727.
    SMRi Q54727. Positions 39-696.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 170187.SP_1687.

    Protein family/group databases

    CAZyi CBM40. Carbohydrate-Binding Module Family 40.
    GH33. Glycoside Hydrolase Family 33.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK75766 ; AAK75766 ; SP_1687 .
    GeneIDi 931147.
    KEGGi spn:SP_1687.
    PATRICi 19707809. VBIStrPne105772_1747.

    Phylogenomic databases

    eggNOGi COG4409.
    HOGENOMi HOG000285031.
    KOi K01186.
    OMAi WRYHYDI.
    OrthoDBi EOG61CM2G.

    Enzyme and pathway databases

    BioCyci SPNE170187:GHGN-1694-MONOMER.
    BRENDAi 3.2.1.18. 5946.
    SABIO-RK Q54727.

    Miscellaneous databases

    EvolutionaryTracei Q54727.

    Family and domain databases

    Gene3Di 2.120.10.10. 2 hits.
    2.40.220.10. 1 hit.
    2.60.120.200. 1 hit.
    InterProi IPR002860. BNR_rpt.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR004124. Glyco_hydro_33_N.
    IPR011040. Sialidases.
    IPR023364. Trans_sialidase_dom3.
    [Graphical view ]
    Pfami PF02012. BNR. 1 hit.
    PF02973. Sialidase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49899. SSF49899. 1 hit.
    SSF50939. SSF50939. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of nanB, a second Streptococcus pneumoniae neuraminidase gene, and purification of the NanB enzyme from recombinant Escherichia coli."
      Berry A.M., Lock R.A., Paton J.C.
      J. Bacteriol. 178:4854-4860(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Serotype 6.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-334 / TIGR4.

    Entry informationi

    Entry nameiNANB_STRPN
    AccessioniPrimary (citable) accession number: Q54727
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: September 26, 2001
    Last modified: October 1, 2014
    This is version 108 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3