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Q54727

- NANB_STRPN

UniProt

Q54727 - NANB_STRPN

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Protein
Sialidase B
Gene
nanB, SP_1687
Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei245 – 2451Substrate By similarity
Active sitei270 – 2701Proton acceptor By similarity
Active sitei541 – 5411 Reviewed prediction
Binding sitei557 – 5571Substrate By similarity
Binding sitei619 – 6191Substrate By similarity
Active sitei653 – 6531Nucleophile By similarity

GO - Molecular functioni

  1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
  2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
  3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciSPNE170187:GHGN-1694-MONOMER.
BRENDAi3.2.1.18. 5946.
SABIO-RKQ54727.

Protein family/group databases

CAZyiCBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase B (EC:3.2.1.18)
Alternative name(s):
Neuraminidase B
Gene namesi
Name:nanB
Ordered Locus Names:SP_1687
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000585: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929
Add
BLAST
Chaini30 – 697668Sialidase B
PRO_0000012038Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi170187.SP_1687.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi42 – 5312
Helixi58 – 614
Helixi62 – 643
Beta strandi70 – 778
Beta strandi83 – 919
Beta strandi101 – 1077
Beta strandi112 – 1187
Turni119 – 1224
Beta strandi123 – 1319
Beta strandi136 – 1416
Beta strandi144 – 1518
Turni152 – 1554
Beta strandi156 – 1616
Beta strandi164 – 1707
Helixi177 – 1793
Beta strandi185 – 1939
Beta strandi196 – 1994
Beta strandi202 – 21312
Helixi217 – 2215
Beta strandi242 – 2454
Beta strandi257 – 26610
Beta strandi271 – 2733
Beta strandi275 – 28410
Beta strandi293 – 2964
Turni312 – 3165
Beta strandi324 – 3329
Turni334 – 3363
Beta strandi339 – 3468
Turni352 – 3543
Beta strandi361 – 37515
Beta strandi383 – 3853
Helixi387 – 3893
Beta strandi390 – 3934
Turni394 – 3974
Beta strandi398 – 4047
Beta strandi408 – 4125
Beta strandi418 – 4258
Beta strandi432 – 44110
Beta strandi446 – 4483
Beta strandi450 – 4523
Beta strandi458 – 4669
Beta strandi467 – 4693
Beta strandi483 – 4853
Beta strandi492 – 4943
Beta strandi499 – 5024
Beta strandi504 – 5107
Beta strandi513 – 5219
Beta strandi527 – 5326
Beta strandi542 – 5487
Beta strandi551 – 5566
Beta strandi559 – 5624
Beta strandi564 – 5707
Beta strandi573 – 5753
Beta strandi594 – 6029
Beta strandi605 – 6139
Beta strandi616 – 6205
Beta strandi622 – 6298
Turni631 – 6333
Beta strandi636 – 64510
Beta strandi653 – 6586
Beta strandi664 – 6696
Beta strandi676 – 6783
Beta strandi685 – 6906
Helixi692 – 6954

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JKBX-ray1.54A30-697[»]
2VW0X-ray2.30A1-697[»]
2VW1X-ray2.39A1-697[»]
2VW2X-ray1.70A1-697[»]
4FOQX-ray1.99A1-697[»]
4FOVX-ray2.29A1-697[»]
4FOWX-ray2.10A1-697[»]
4FOYX-ray1.84A1-697[»]
4FP2X-ray2.05A1-697[»]
4FP3X-ray2.74A1-697[»]
4FPCX-ray2.32A1-697[»]
4FPEX-ray2.18A1-697[»]
4FPFX-ray2.23A1-697[»]
4FPGX-ray2.58A1-697[»]
4FPHX-ray1.94A1-697[»]
4FPJX-ray1.98A1-697[»]
4FPKX-ray2.40A1-697[»]
4FPLX-ray2.10A1-697[»]
4FPOX-ray2.59B1-697[»]
4FPYX-ray2.18A1-697[»]
4FQ4X-ray1.84A1-697[»]
ProteinModelPortaliQ54727.
SMRiQ54727. Positions 39-696.

Miscellaneous databases

EvolutionaryTraceiQ54727.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati280 – 29112BNR 1
Add
BLAST
Repeati462 – 47312BNR 2
Add
BLAST
Repeati517 – 52812BNR 3
Add
BLAST
Repeati566 – 57712BNR 4
Add
BLAST

Sequence similaritiesi

Contains 4 BNR repeats.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG4409.
HOGENOMiHOG000285031.
KOiK01186.
OMAiWRYHYDI.
OrthoDBiEOG61CM2G.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
2.40.220.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR002860. BNR_rpt.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR004124. Glyco_hydro_33_N.
IPR011040. Sialidases.
IPR023364. Trans_sialidase_dom3.
[Graphical view]
PfamiPF02012. BNR. 1 hit.
PF02973. Sialidase. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF50939. SSF50939. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54727-1 [UniParc]FASTAAdd to Basket

« Hide

MNKRGLYSKL GISVVGISLL MGVPTLIHAN ELNYGQLSIS PIFQGGSYQL    50
NNKSIDISSL LLDKLSGESQ TVVMKFKADK PNSLQALFGL SNSKAGFKNN 100
YFSIFMRDSG EIGVEIRDAQ KGINYLFSRP ASLWGKHKGQ AVENTLVFVS 150
DSKDKTYTMY VNGIEVFSET VDTFLPISNI NGIDKATLGA VNREGKEHYL 200
AKGSIDEISL FNKAISDQEV STIPLSNPFQ LIFQSGDSTQ ANYFRIPTLY 250
TLSSGRVLSS IDARYGGTHD SKSKINIATS YSDDNGKTWS EPIFAMKFND 300
YEEQLVYWPR DNKLKNSQIS GSASFIDSSI VEDKKSGKTI LLADVMPAGI 350
GNNNANKADS GFKEINGHYY LKLKKNGDND FRYTVRENGV VYNETTNKPT 400
NYTINDKYEV LEGGKSLTVE QYSVDFDSGS LRERHNGKQV PMNVFYKDSL 450
FKVTPTNYIA MTTSQNRGES WEQFKLLPPF LGEKHNGTYL CPGQGLALKS 500
SNRLIFATYT SGELTYLISD DSGQTWKKSS ASIPFKNATA EAQMVELRDG 550
VIRTFFRTTT GKIAYMTSRD SGETWSKVSY IDGIQQTSYG TQVSAIKYSQ 600
LIDGKEAVIL STPNSRSGRK GGQLVVGLVN KEDDSIDWKY HYDIDLPSYG 650
YAYSAITELP NHHIGVLFEK YDSWSRNELH LSNVVQYIDL EINDLTK 697
Length:697
Mass (Da):77,695
Last modified:September 26, 2001 - v2
Checksum:iA0D9F9AFDE46AFD7
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti121 – 1211K → E in AAC44396. 1 Publication
Sequence conflicti206 – 2061D → G in AAC44396. 1 Publication
Sequence conflicti222 – 2221T → N in AAC44396. 1 Publication
Sequence conflicti393 – 3931N → D in AAC44396. 1 Publication
Sequence conflicti639 – 6391K → R in AAC44396. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U43526 Genomic DNA. Translation: AAC44396.1.
AE005672 Genomic DNA. Translation: AAK75766.1.
PIRiE95196.
RefSeqiNP_346126.1. NC_003028.3.

Genome annotation databases

EnsemblBacteriaiAAK75766; AAK75766; SP_1687.
GeneIDi931147.
KEGGispn:SP_1687.
PATRICi19707809. VBIStrPne105772_1747.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U43526 Genomic DNA. Translation: AAC44396.1 .
AE005672 Genomic DNA. Translation: AAK75766.1 .
PIRi E95196.
RefSeqi NP_346126.1. NC_003028.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2JKB X-ray 1.54 A 30-697 [» ]
2VW0 X-ray 2.30 A 1-697 [» ]
2VW1 X-ray 2.39 A 1-697 [» ]
2VW2 X-ray 1.70 A 1-697 [» ]
4FOQ X-ray 1.99 A 1-697 [» ]
4FOV X-ray 2.29 A 1-697 [» ]
4FOW X-ray 2.10 A 1-697 [» ]
4FOY X-ray 1.84 A 1-697 [» ]
4FP2 X-ray 2.05 A 1-697 [» ]
4FP3 X-ray 2.74 A 1-697 [» ]
4FPC X-ray 2.32 A 1-697 [» ]
4FPE X-ray 2.18 A 1-697 [» ]
4FPF X-ray 2.23 A 1-697 [» ]
4FPG X-ray 2.58 A 1-697 [» ]
4FPH X-ray 1.94 A 1-697 [» ]
4FPJ X-ray 1.98 A 1-697 [» ]
4FPK X-ray 2.40 A 1-697 [» ]
4FPL X-ray 2.10 A 1-697 [» ]
4FPO X-ray 2.59 B 1-697 [» ]
4FPY X-ray 2.18 A 1-697 [» ]
4FQ4 X-ray 1.84 A 1-697 [» ]
ProteinModelPortali Q54727.
SMRi Q54727. Positions 39-696.
ModBasei Search...

Protein-protein interaction databases

STRINGi 170187.SP_1687.

Protein family/group databases

CAZyi CBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK75766 ; AAK75766 ; SP_1687 .
GeneIDi 931147.
KEGGi spn:SP_1687.
PATRICi 19707809. VBIStrPne105772_1747.

Phylogenomic databases

eggNOGi COG4409.
HOGENOMi HOG000285031.
KOi K01186.
OMAi WRYHYDI.
OrthoDBi EOG61CM2G.

Enzyme and pathway databases

BioCyci SPNE170187:GHGN-1694-MONOMER.
BRENDAi 3.2.1.18. 5946.
SABIO-RK Q54727.

Miscellaneous databases

EvolutionaryTracei Q54727.

Family and domain databases

Gene3Di 2.120.10.10. 2 hits.
2.40.220.10. 1 hit.
2.60.120.200. 1 hit.
InterProi IPR002860. BNR_rpt.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR004124. Glyco_hydro_33_N.
IPR011040. Sialidases.
IPR023364. Trans_sialidase_dom3.
[Graphical view ]
Pfami PF02012. BNR. 1 hit.
PF02973. Sialidase. 1 hit.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 1 hit.
SSF50939. SSF50939. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of nanB, a second Streptococcus pneumoniae neuraminidase gene, and purification of the NanB enzyme from recombinant Escherichia coli."
    Berry A.M., Lock R.A., Paton J.C.
    J. Bacteriol. 178:4854-4860(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Serotype 6.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-334 / TIGR4.

Entry informationi

Entry nameiNANB_STRPN
AccessioniPrimary (citable) accession number: Q54727
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 26, 2001
Last modified: June 11, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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