Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sialidase B

Gene

nanB

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei245SubstrateBy similarity1
Active sitei270Proton acceptorBy similarity1
Active sitei541Sequence analysis1
Binding sitei557SubstrateBy similarity1
Binding sitei619SubstrateBy similarity1
Active sitei653NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.18. 1960.
SABIO-RKQ54727.

Protein family/group databases

CAZyiCBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase B (EC:3.2.1.18)
Alternative name(s):
Neuraminidase B
Gene namesi
Name:nanB
Ordered Locus Names:SP_1687
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000585 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Add BLAST29
ChainiPRO_000001203830 – 697Sialidase BAdd BLAST668

Interactioni

Protein-protein interaction databases

STRINGi170187.SP_1687.

Structurei

Secondary structure

1697
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi42 – 53Combined sources12
Helixi58 – 61Combined sources4
Turni62 – 64Combined sources3
Beta strandi70 – 77Combined sources8
Beta strandi83 – 91Combined sources9
Beta strandi101 – 107Combined sources7
Beta strandi112 – 118Combined sources7
Turni119 – 122Combined sources4
Beta strandi123 – 131Combined sources9
Beta strandi136 – 141Combined sources6
Beta strandi144 – 151Combined sources8
Turni152 – 155Combined sources4
Beta strandi156 – 161Combined sources6
Beta strandi164 – 170Combined sources7
Helixi177 – 179Combined sources3
Beta strandi185 – 193Combined sources9
Beta strandi196 – 199Combined sources4
Beta strandi202 – 213Combined sources12
Helixi217 – 220Combined sources4
Beta strandi242 – 245Combined sources4
Beta strandi257 – 266Combined sources10
Beta strandi271 – 273Combined sources3
Beta strandi275 – 284Combined sources10
Beta strandi293 – 296Combined sources4
Turni312 – 316Combined sources5
Beta strandi324 – 332Combined sources9
Turni334 – 336Combined sources3
Beta strandi339 – 346Combined sources8
Turni352 – 354Combined sources3
Beta strandi361 – 365Combined sources5
Beta strandi368 – 375Combined sources8
Beta strandi383 – 385Combined sources3
Helixi387 – 389Combined sources3
Beta strandi390 – 393Combined sources4
Turni394 – 397Combined sources4
Beta strandi398 – 404Combined sources7
Beta strandi408 – 412Combined sources5
Beta strandi418 – 425Combined sources8
Beta strandi432 – 441Combined sources10
Beta strandi446 – 448Combined sources3
Beta strandi450 – 452Combined sources3
Beta strandi458 – 466Combined sources9
Beta strandi467 – 469Combined sources3
Beta strandi483 – 485Combined sources3
Beta strandi492 – 494Combined sources3
Beta strandi499 – 502Combined sources4
Beta strandi504 – 510Combined sources7
Beta strandi513 – 521Combined sources9
Beta strandi527 – 532Combined sources6
Beta strandi542 – 548Combined sources7
Beta strandi551 – 556Combined sources6
Beta strandi559 – 562Combined sources4
Beta strandi564 – 570Combined sources7
Beta strandi573 – 575Combined sources3
Beta strandi594 – 597Combined sources4
Beta strandi607 – 613Combined sources7
Beta strandi616 – 620Combined sources5
Beta strandi622 – 629Combined sources8
Turni631 – 633Combined sources3
Beta strandi636 – 645Combined sources10
Beta strandi653 – 658Combined sources6
Beta strandi664 – 669Combined sources6
Beta strandi676 – 678Combined sources3
Beta strandi685 – 690Combined sources6
Helixi692 – 695Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JKBX-ray1.54A30-697[»]
2VW0X-ray2.30A1-697[»]
2VW1X-ray2.39A1-697[»]
2VW2X-ray1.70A1-697[»]
4FOQX-ray1.99A1-697[»]
4FOVX-ray2.29A1-697[»]
4FOWX-ray2.10A1-697[»]
4FOYX-ray1.84A1-697[»]
4FP2X-ray2.05A1-697[»]
4FP3X-ray2.74A1-697[»]
4FPCX-ray2.32A1-697[»]
4FPEX-ray2.18A1-697[»]
4FPFX-ray2.23A1-697[»]
4FPGX-ray2.58A1-697[»]
4FPHX-ray1.94A1-697[»]
4FPJX-ray1.98A1-697[»]
4FPKX-ray2.40A1-697[»]
4FPLX-ray2.10A1-697[»]
4FPOX-ray2.59B1-697[»]
4FPYX-ray2.18A1-697[»]
4FQ4X-ray1.84A1-697[»]
4XE9X-ray1.84A39-696[»]
4XHBX-ray1.88A39-696[»]
4XHXX-ray2.10A39-696[»]
4XIKX-ray1.91A39-696[»]
4XILX-ray1.90A39-696[»]
4XIOX-ray1.80A39-696[»]
4XJ8X-ray2.75A39-696[»]
4XJ9X-ray1.98A39-696[»]
4XJAX-ray1.98A39-696[»]
4XJUX-ray1.94A39-696[»]
4XJWX-ray1.53A39-696[»]
4XJZX-ray1.56A39-696[»]
4XMAX-ray2.09A39-696[»]
4XMIX-ray1.97A39-696[»]
4XOGX-ray2.09A37-694[»]
4XYXX-ray2.10A40-697[»]
ProteinModelPortaliQ54727.
SMRiQ54727.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ54727.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati280 – 291BNR 1Add BLAST12
Repeati462 – 473BNR 2Add BLAST12
Repeati517 – 528BNR 3Add BLAST12
Repeati566 – 577BNR 4Add BLAST12

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated
Contains 4 BNR repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG4409. LUCA.
HOGENOMiHOG000285031.
KOiK01186.
OMAiIFLMADM.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
2.40.220.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR002860. BNR_rpt.
IPR013320. ConA-like_dom.
IPR004124. Glyco_hydro_33_N.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
IPR023364. Trans_sialidase_dom3.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 2 hits.
PfamiPF02012. BNR. 1 hit.
PF13088. BNR_2. 1 hit.
PF02973. Sialidase. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF50939. SSF50939. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54727-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKRGLYSKL GISVVGISLL MGVPTLIHAN ELNYGQLSIS PIFQGGSYQL
60 70 80 90 100
NNKSIDISSL LLDKLSGESQ TVVMKFKADK PNSLQALFGL SNSKAGFKNN
110 120 130 140 150
YFSIFMRDSG EIGVEIRDAQ KGINYLFSRP ASLWGKHKGQ AVENTLVFVS
160 170 180 190 200
DSKDKTYTMY VNGIEVFSET VDTFLPISNI NGIDKATLGA VNREGKEHYL
210 220 230 240 250
AKGSIDEISL FNKAISDQEV STIPLSNPFQ LIFQSGDSTQ ANYFRIPTLY
260 270 280 290 300
TLSSGRVLSS IDARYGGTHD SKSKINIATS YSDDNGKTWS EPIFAMKFND
310 320 330 340 350
YEEQLVYWPR DNKLKNSQIS GSASFIDSSI VEDKKSGKTI LLADVMPAGI
360 370 380 390 400
GNNNANKADS GFKEINGHYY LKLKKNGDND FRYTVRENGV VYNETTNKPT
410 420 430 440 450
NYTINDKYEV LEGGKSLTVE QYSVDFDSGS LRERHNGKQV PMNVFYKDSL
460 470 480 490 500
FKVTPTNYIA MTTSQNRGES WEQFKLLPPF LGEKHNGTYL CPGQGLALKS
510 520 530 540 550
SNRLIFATYT SGELTYLISD DSGQTWKKSS ASIPFKNATA EAQMVELRDG
560 570 580 590 600
VIRTFFRTTT GKIAYMTSRD SGETWSKVSY IDGIQQTSYG TQVSAIKYSQ
610 620 630 640 650
LIDGKEAVIL STPNSRSGRK GGQLVVGLVN KEDDSIDWKY HYDIDLPSYG
660 670 680 690
YAYSAITELP NHHIGVLFEK YDSWSRNELH LSNVVQYIDL EINDLTK
Length:697
Mass (Da):77,695
Last modified:September 26, 2001 - v2
Checksum:iA0D9F9AFDE46AFD7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti121K → E in AAC44396 (PubMed:8759848).Curated1
Sequence conflicti206D → G in AAC44396 (PubMed:8759848).Curated1
Sequence conflicti222T → N in AAC44396 (PubMed:8759848).Curated1
Sequence conflicti393N → D in AAC44396 (PubMed:8759848).Curated1
Sequence conflicti639K → R in AAC44396 (PubMed:8759848).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43526 Genomic DNA. Translation: AAC44396.1.
AE005672 Genomic DNA. Translation: AAK75766.1.
PIRiE95196.
RefSeqiWP_001042179.1. NZ_AKVY01000001.1.

Genome annotation databases

EnsemblBacteriaiAAK75766; AAK75766; SP_1687.
KEGGispn:SP_1687.
PATRICi19707809. VBIStrPne105772_1747.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43526 Genomic DNA. Translation: AAC44396.1.
AE005672 Genomic DNA. Translation: AAK75766.1.
PIRiE95196.
RefSeqiWP_001042179.1. NZ_AKVY01000001.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JKBX-ray1.54A30-697[»]
2VW0X-ray2.30A1-697[»]
2VW1X-ray2.39A1-697[»]
2VW2X-ray1.70A1-697[»]
4FOQX-ray1.99A1-697[»]
4FOVX-ray2.29A1-697[»]
4FOWX-ray2.10A1-697[»]
4FOYX-ray1.84A1-697[»]
4FP2X-ray2.05A1-697[»]
4FP3X-ray2.74A1-697[»]
4FPCX-ray2.32A1-697[»]
4FPEX-ray2.18A1-697[»]
4FPFX-ray2.23A1-697[»]
4FPGX-ray2.58A1-697[»]
4FPHX-ray1.94A1-697[»]
4FPJX-ray1.98A1-697[»]
4FPKX-ray2.40A1-697[»]
4FPLX-ray2.10A1-697[»]
4FPOX-ray2.59B1-697[»]
4FPYX-ray2.18A1-697[»]
4FQ4X-ray1.84A1-697[»]
4XE9X-ray1.84A39-696[»]
4XHBX-ray1.88A39-696[»]
4XHXX-ray2.10A39-696[»]
4XIKX-ray1.91A39-696[»]
4XILX-ray1.90A39-696[»]
4XIOX-ray1.80A39-696[»]
4XJ8X-ray2.75A39-696[»]
4XJ9X-ray1.98A39-696[»]
4XJAX-ray1.98A39-696[»]
4XJUX-ray1.94A39-696[»]
4XJWX-ray1.53A39-696[»]
4XJZX-ray1.56A39-696[»]
4XMAX-ray2.09A39-696[»]
4XMIX-ray1.97A39-696[»]
4XOGX-ray2.09A37-694[»]
4XYXX-ray2.10A40-697[»]
ProteinModelPortaliQ54727.
SMRiQ54727.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi170187.SP_1687.

Protein family/group databases

CAZyiCBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK75766; AAK75766; SP_1687.
KEGGispn:SP_1687.
PATRICi19707809. VBIStrPne105772_1747.

Phylogenomic databases

eggNOGiCOG4409. LUCA.
HOGENOMiHOG000285031.
KOiK01186.
OMAiIFLMADM.

Enzyme and pathway databases

BRENDAi3.2.1.18. 1960.
SABIO-RKQ54727.

Miscellaneous databases

EvolutionaryTraceiQ54727.
PROiQ54727.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
2.40.220.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR002860. BNR_rpt.
IPR013320. ConA-like_dom.
IPR004124. Glyco_hydro_33_N.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
IPR023364. Trans_sialidase_dom3.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 2 hits.
PfamiPF02012. BNR. 1 hit.
PF13088. BNR_2. 1 hit.
PF02973. Sialidase. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF50939. SSF50939. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiNANB_STRPN
AccessioniPrimary (citable) accession number: Q54727
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 26, 2001
Last modified: November 2, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.