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Q54727 (NANB_STRPN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sialidase B

EC=3.2.1.18
Alternative name(s):
Neuraminidase B
Gene names
Name:nanB
Ordered Locus Names:SP_1687
OrganismStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) [Complete proteome] [HAMAP]
Taxonomic identifier170187 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length697 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Sequence similarities

Belongs to the glycosyl hydrolase 33 family.

Contains 4 BNR repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929
Chain30 – 697668Sialidase B
PRO_0000012038

Regions

Repeat280 – 29112BNR 1
Repeat462 – 47312BNR 2
Repeat517 – 52812BNR 3
Repeat566 – 57712BNR 4

Sites

Active site2701Proton acceptor By similarity
Active site5411 Potential
Active site6531Nucleophile By similarity
Binding site2451Substrate By similarity
Binding site5571Substrate By similarity
Binding site6191Substrate By similarity

Experimental info

Sequence conflict1211K → E in AAC44396. Ref.1
Sequence conflict2061D → G in AAC44396. Ref.1
Sequence conflict2221T → N in AAC44396. Ref.1
Sequence conflict3931N → D in AAC44396. Ref.1
Sequence conflict6391K → R in AAC44396. Ref.1

Secondary structure

........................................................................................................................ 697
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q54727 [UniParc].

Last modified September 26, 2001. Version 2.
Checksum: A0D9F9AFDE46AFD7

FASTA69777,695
        10         20         30         40         50         60 
MNKRGLYSKL GISVVGISLL MGVPTLIHAN ELNYGQLSIS PIFQGGSYQL NNKSIDISSL 

        70         80         90        100        110        120 
LLDKLSGESQ TVVMKFKADK PNSLQALFGL SNSKAGFKNN YFSIFMRDSG EIGVEIRDAQ 

       130        140        150        160        170        180 
KGINYLFSRP ASLWGKHKGQ AVENTLVFVS DSKDKTYTMY VNGIEVFSET VDTFLPISNI 

       190        200        210        220        230        240 
NGIDKATLGA VNREGKEHYL AKGSIDEISL FNKAISDQEV STIPLSNPFQ LIFQSGDSTQ 

       250        260        270        280        290        300 
ANYFRIPTLY TLSSGRVLSS IDARYGGTHD SKSKINIATS YSDDNGKTWS EPIFAMKFND 

       310        320        330        340        350        360 
YEEQLVYWPR DNKLKNSQIS GSASFIDSSI VEDKKSGKTI LLADVMPAGI GNNNANKADS 

       370        380        390        400        410        420 
GFKEINGHYY LKLKKNGDND FRYTVRENGV VYNETTNKPT NYTINDKYEV LEGGKSLTVE 

       430        440        450        460        470        480 
QYSVDFDSGS LRERHNGKQV PMNVFYKDSL FKVTPTNYIA MTTSQNRGES WEQFKLLPPF 

       490        500        510        520        530        540 
LGEKHNGTYL CPGQGLALKS SNRLIFATYT SGELTYLISD DSGQTWKKSS ASIPFKNATA 

       550        560        570        580        590        600 
EAQMVELRDG VIRTFFRTTT GKIAYMTSRD SGETWSKVSY IDGIQQTSYG TQVSAIKYSQ 

       610        620        630        640        650        660 
LIDGKEAVIL STPNSRSGRK GGQLVVGLVN KEDDSIDWKY HYDIDLPSYG YAYSAITELP 

       670        680        690 
NHHIGVLFEK YDSWSRNELH LSNVVQYIDL EINDLTK 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of nanB, a second Streptococcus pneumoniae neuraminidase gene, and purification of the NanB enzyme from recombinant Escherichia coli."
Berry A.M., Lock R.A., Paton J.C.
J. Bacteriol. 178:4854-4860(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Serotype 6.
[2]"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae."
Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. expand/collapse author list , Lewis M.R., Radune D., Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M.
Science 293:498-506(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-334 / TIGR4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U43526 Genomic DNA. Translation: AAC44396.1.
AE005672 Genomic DNA. Translation: AAK75766.1.
PIRE95196.
RefSeqNP_346126.1. NC_003028.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2JKBX-ray1.54A30-697[»]
2VW0X-ray2.30A1-697[»]
2VW1X-ray2.39A1-697[»]
2VW2X-ray1.70A1-697[»]
4FOQX-ray1.99A1-697[»]
4FOVX-ray2.29A1-697[»]
4FOWX-ray2.10A1-697[»]
4FOYX-ray1.84A1-697[»]
4FP2X-ray2.05A1-697[»]
4FP3X-ray2.74A1-697[»]
4FPCX-ray2.32A1-697[»]
4FPEX-ray2.18A1-697[»]
4FPFX-ray2.23A1-697[»]
4FPGX-ray2.58A1-697[»]
4FPHX-ray1.94A1-697[»]
4FPJX-ray1.98A1-697[»]
4FPKX-ray2.40A1-697[»]
4FPLX-ray2.10A1-697[»]
4FPOX-ray2.59B1-697[»]
4FPYX-ray2.18A1-697[»]
4FQ4X-ray1.84A1-697[»]
ProteinModelPortalQ54727.
SMRQ54727. Positions 39-696.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING170187.SP_1687.

Protein family/group databases

CAZyCBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK75766; AAK75766; SP_1687.
GeneID931147.
KEGGspn:SP_1687.
PATRIC19707809. VBIStrPne105772_1747.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG4409.
HOGENOMHOG000285031.
KOK01186.
OMAWRYHYDI.
OrthoDBEOG61CM2G.

Enzyme and pathway databases

BioCycSPNE170187:GHGN-1694-MONOMER.
BRENDA3.2.1.18. 5946.
SABIO-RKQ54727.

Family and domain databases

Gene3D2.120.10.10. 2 hits.
2.40.220.10. 1 hit.
2.60.120.200. 1 hit.
InterProIPR002860. BNR_rpt.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR004124. Glyco_hydro_33_N.
IPR011040. Sialidases.
IPR023364. Trans_sialidase_dom3.
[Graphical view]
PfamPF02012. BNR. 1 hit.
PF02973. Sialidase. 1 hit.
[Graphical view]
SUPFAMSSF49899. SSF49899. 1 hit.
SSF50939. SSF50939. 2 hits.
ProtoNetSearch...

Other

EvolutionaryTraceQ54727.

Entry information

Entry nameNANB_STRPN
AccessionPrimary (citable) accession number: Q54727
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 26, 2001
Last modified: June 11, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries