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Q54727

- NANB_STRPN

UniProt

Q54727 - NANB_STRPN

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Protein

Sialidase B

Gene

nanB

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei245 – 2451SubstrateBy similarity
Active sitei270 – 2701Proton acceptorBy similarity
Active sitei541 – 5411Sequence Analysis
Binding sitei557 – 5571SubstrateBy similarity
Binding sitei619 – 6191SubstrateBy similarity
Active sitei653 – 6531NucleophileBy similarity

GO - Molecular functioni

  1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
  2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
  3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciSPNE170187:GHGN-1694-MONOMER.
BRENDAi3.2.1.18. 5946.
SABIO-RKQ54727.

Protein family/group databases

CAZyiCBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase B (EC:3.2.1.18)
Alternative name(s):
Neuraminidase B
Gene namesi
Name:nanB
Ordered Locus Names:SP_1687
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000585: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Add
BLAST
Chaini30 – 697668Sialidase BPRO_0000012038Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi170187.SP_1687.

Structurei

Secondary structure

1
697
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi42 – 5312Combined sources
Helixi58 – 614Combined sources
Helixi62 – 643Combined sources
Beta strandi70 – 778Combined sources
Beta strandi83 – 919Combined sources
Beta strandi101 – 1077Combined sources
Beta strandi112 – 1187Combined sources
Turni119 – 1224Combined sources
Beta strandi123 – 1319Combined sources
Beta strandi136 – 1416Combined sources
Beta strandi144 – 1518Combined sources
Turni152 – 1554Combined sources
Beta strandi156 – 1616Combined sources
Beta strandi164 – 1707Combined sources
Helixi177 – 1793Combined sources
Beta strandi185 – 1939Combined sources
Beta strandi196 – 1994Combined sources
Beta strandi202 – 21312Combined sources
Helixi217 – 2215Combined sources
Beta strandi242 – 2454Combined sources
Beta strandi257 – 26610Combined sources
Beta strandi271 – 2733Combined sources
Beta strandi275 – 28410Combined sources
Beta strandi293 – 2964Combined sources
Turni312 – 3165Combined sources
Beta strandi324 – 3329Combined sources
Turni334 – 3363Combined sources
Beta strandi339 – 3468Combined sources
Turni352 – 3543Combined sources
Beta strandi361 – 37515Combined sources
Beta strandi383 – 3853Combined sources
Helixi387 – 3893Combined sources
Beta strandi390 – 3934Combined sources
Turni394 – 3974Combined sources
Beta strandi398 – 4047Combined sources
Beta strandi408 – 4125Combined sources
Beta strandi418 – 4258Combined sources
Beta strandi432 – 44110Combined sources
Beta strandi446 – 4483Combined sources
Beta strandi450 – 4523Combined sources
Beta strandi458 – 4669Combined sources
Beta strandi467 – 4693Combined sources
Beta strandi483 – 4853Combined sources
Beta strandi492 – 4943Combined sources
Beta strandi499 – 5024Combined sources
Beta strandi504 – 5107Combined sources
Beta strandi513 – 5219Combined sources
Beta strandi527 – 5326Combined sources
Beta strandi542 – 5487Combined sources
Beta strandi551 – 5566Combined sources
Beta strandi559 – 5624Combined sources
Beta strandi564 – 5707Combined sources
Beta strandi573 – 5753Combined sources
Beta strandi594 – 6029Combined sources
Beta strandi605 – 6139Combined sources
Beta strandi616 – 6205Combined sources
Beta strandi622 – 6298Combined sources
Turni631 – 6333Combined sources
Beta strandi636 – 64510Combined sources
Beta strandi653 – 6586Combined sources
Beta strandi664 – 6696Combined sources
Beta strandi676 – 6783Combined sources
Beta strandi685 – 6906Combined sources
Helixi692 – 6954Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JKBX-ray1.54A30-697[»]
2VW0X-ray2.30A1-697[»]
2VW1X-ray2.39A1-697[»]
2VW2X-ray1.70A1-697[»]
4FOQX-ray1.99A1-697[»]
4FOVX-ray2.29A1-697[»]
4FOWX-ray2.10A1-697[»]
4FOYX-ray1.84A1-697[»]
4FP2X-ray2.05A1-697[»]
4FP3X-ray2.74A1-697[»]
4FPCX-ray2.32A1-697[»]
4FPEX-ray2.18A1-697[»]
4FPFX-ray2.23A1-697[»]
4FPGX-ray2.58A1-697[»]
4FPHX-ray1.94A1-697[»]
4FPJX-ray1.98A1-697[»]
4FPKX-ray2.40A1-697[»]
4FPLX-ray2.10A1-697[»]
4FPOX-ray2.59B1-697[»]
4FPYX-ray2.18A1-697[»]
4FQ4X-ray1.84A1-697[»]
ProteinModelPortaliQ54727.
SMRiQ54727. Positions 39-696.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ54727.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati280 – 29112BNR 1Add
BLAST
Repeati462 – 47312BNR 2Add
BLAST
Repeati517 – 52812BNR 3Add
BLAST
Repeati566 – 57712BNR 4Add
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated
Contains 4 BNR repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG4409.
HOGENOMiHOG000285031.
KOiK01186.
OMAiWRYHYDI.
OrthoDBiEOG61CM2G.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
2.40.220.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR002860. BNR_rpt.
IPR013320. ConA-like_dom.
IPR004124. Glyco_hydro_33_N.
IPR011040. Sialidases.
IPR023364. Trans_sialidase_dom3.
[Graphical view]
PfamiPF02012. BNR. 1 hit.
PF02973. Sialidase. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF50939. SSF50939. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54727-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKRGLYSKL GISVVGISLL MGVPTLIHAN ELNYGQLSIS PIFQGGSYQL
60 70 80 90 100
NNKSIDISSL LLDKLSGESQ TVVMKFKADK PNSLQALFGL SNSKAGFKNN
110 120 130 140 150
YFSIFMRDSG EIGVEIRDAQ KGINYLFSRP ASLWGKHKGQ AVENTLVFVS
160 170 180 190 200
DSKDKTYTMY VNGIEVFSET VDTFLPISNI NGIDKATLGA VNREGKEHYL
210 220 230 240 250
AKGSIDEISL FNKAISDQEV STIPLSNPFQ LIFQSGDSTQ ANYFRIPTLY
260 270 280 290 300
TLSSGRVLSS IDARYGGTHD SKSKINIATS YSDDNGKTWS EPIFAMKFND
310 320 330 340 350
YEEQLVYWPR DNKLKNSQIS GSASFIDSSI VEDKKSGKTI LLADVMPAGI
360 370 380 390 400
GNNNANKADS GFKEINGHYY LKLKKNGDND FRYTVRENGV VYNETTNKPT
410 420 430 440 450
NYTINDKYEV LEGGKSLTVE QYSVDFDSGS LRERHNGKQV PMNVFYKDSL
460 470 480 490 500
FKVTPTNYIA MTTSQNRGES WEQFKLLPPF LGEKHNGTYL CPGQGLALKS
510 520 530 540 550
SNRLIFATYT SGELTYLISD DSGQTWKKSS ASIPFKNATA EAQMVELRDG
560 570 580 590 600
VIRTFFRTTT GKIAYMTSRD SGETWSKVSY IDGIQQTSYG TQVSAIKYSQ
610 620 630 640 650
LIDGKEAVIL STPNSRSGRK GGQLVVGLVN KEDDSIDWKY HYDIDLPSYG
660 670 680 690
YAYSAITELP NHHIGVLFEK YDSWSRNELH LSNVVQYIDL EINDLTK
Length:697
Mass (Da):77,695
Last modified:September 26, 2001 - v2
Checksum:iA0D9F9AFDE46AFD7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti121 – 1211K → E in AAC44396. (PubMed:8759848)Curated
Sequence conflicti206 – 2061D → G in AAC44396. (PubMed:8759848)Curated
Sequence conflicti222 – 2221T → N in AAC44396. (PubMed:8759848)Curated
Sequence conflicti393 – 3931N → D in AAC44396. (PubMed:8759848)Curated
Sequence conflicti639 – 6391K → R in AAC44396. (PubMed:8759848)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43526 Genomic DNA. Translation: AAC44396.1.
AE005672 Genomic DNA. Translation: AAK75766.1.
PIRiE95196.
RefSeqiNP_346126.1. NC_003028.3.

Genome annotation databases

EnsemblBacteriaiAAK75766; AAK75766; SP_1687.
GeneIDi931147.
KEGGispn:SP_1687.
PATRICi19707809. VBIStrPne105772_1747.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43526 Genomic DNA. Translation: AAC44396.1 .
AE005672 Genomic DNA. Translation: AAK75766.1 .
PIRi E95196.
RefSeqi NP_346126.1. NC_003028.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2JKB X-ray 1.54 A 30-697 [» ]
2VW0 X-ray 2.30 A 1-697 [» ]
2VW1 X-ray 2.39 A 1-697 [» ]
2VW2 X-ray 1.70 A 1-697 [» ]
4FOQ X-ray 1.99 A 1-697 [» ]
4FOV X-ray 2.29 A 1-697 [» ]
4FOW X-ray 2.10 A 1-697 [» ]
4FOY X-ray 1.84 A 1-697 [» ]
4FP2 X-ray 2.05 A 1-697 [» ]
4FP3 X-ray 2.74 A 1-697 [» ]
4FPC X-ray 2.32 A 1-697 [» ]
4FPE X-ray 2.18 A 1-697 [» ]
4FPF X-ray 2.23 A 1-697 [» ]
4FPG X-ray 2.58 A 1-697 [» ]
4FPH X-ray 1.94 A 1-697 [» ]
4FPJ X-ray 1.98 A 1-697 [» ]
4FPK X-ray 2.40 A 1-697 [» ]
4FPL X-ray 2.10 A 1-697 [» ]
4FPO X-ray 2.59 B 1-697 [» ]
4FPY X-ray 2.18 A 1-697 [» ]
4FQ4 X-ray 1.84 A 1-697 [» ]
ProteinModelPortali Q54727.
SMRi Q54727. Positions 39-696.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 170187.SP_1687.

Protein family/group databases

CAZyi CBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK75766 ; AAK75766 ; SP_1687 .
GeneIDi 931147.
KEGGi spn:SP_1687.
PATRICi 19707809. VBIStrPne105772_1747.

Phylogenomic databases

eggNOGi COG4409.
HOGENOMi HOG000285031.
KOi K01186.
OMAi WRYHYDI.
OrthoDBi EOG61CM2G.

Enzyme and pathway databases

BioCyci SPNE170187:GHGN-1694-MONOMER.
BRENDAi 3.2.1.18. 5946.
SABIO-RK Q54727.

Miscellaneous databases

EvolutionaryTracei Q54727.

Family and domain databases

Gene3Di 2.120.10.10. 2 hits.
2.40.220.10. 1 hit.
2.60.120.200. 1 hit.
InterProi IPR002860. BNR_rpt.
IPR013320. ConA-like_dom.
IPR004124. Glyco_hydro_33_N.
IPR011040. Sialidases.
IPR023364. Trans_sialidase_dom3.
[Graphical view ]
Pfami PF02012. BNR. 1 hit.
PF02973. Sialidase. 1 hit.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 1 hit.
SSF50939. SSF50939. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of nanB, a second Streptococcus pneumoniae neuraminidase gene, and purification of the NanB enzyme from recombinant Escherichia coli."
    Berry A.M., Lock R.A., Paton J.C.
    J. Bacteriol. 178:4854-4860(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Serotype 6.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-334 / TIGR4.

Entry informationi

Entry nameiNANB_STRPN
AccessioniPrimary (citable) accession number: Q54727
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 26, 2001
Last modified: November 26, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3