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Protein

Aclacinomycin methylesterase RdmC

Gene

rdmC

Organism
Streptomyces purpurascens
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the anthracycline aclacinomycin which is an aromatic polyketide antibiotic that exhibits high cytotoxicity and is widely applied in the chemotherapy of a variety of cancers. Catalyzes the removal of the methoxy group from the C-15 position of aclacinomycin T and A to yield 15-demethoxyaclacinomycin T and A, respectively.2 Publications

Catalytic activityi

Aclacinomycin T + H2O = 15-demethylaclacinomycin T + methanol.1 Publication

Kineticsi

  1. KM=15.5 µM for aclacinomycin T (at pH 7.5 and 37 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    Temperature dependencei

    Optimum temperature is 37 degrees Celsius. The activity at 30 and 45 degrees Celsius is 58% and 87% of the optimal activity, respectively. Exceptionally high temperature stability.1 Publication

    Pathwayi: aclacinomycin biosynthesis

    This protein is involved in the pathway aclacinomycin biosynthesis, which is part of Antibiotic biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway aclacinomycin biosynthesis and in Antibiotic biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei102Sequence analysis1
    Active sitei248Sequence analysis1
    Active sitei276Sequence analysis1

    GO - Molecular functioni

    • carboxylic ester hydrolase activity Source: UniProtKB

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-18184.
    BRENDAi3.1.1.95. 6079.
    UniPathwayiUPA01043.

    Protein family/group databases

    ESTHERistrpu-rdmC. Aclacinomycin-methylesterase_RdmC.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aclacinomycin methylesterase RdmC (EC:3.1.1.95)
    Gene namesi
    Name:rdmC
    OrganismiStreptomyces purpurascens
    Taxonomic identifieri1924 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00004257192 – 298Aclacinomycin methylesterase RdmCAdd BLAST297

    Interactioni

    Subunit structurei

    Monomer.2 Publications

    Structurei

    Secondary structure

    1298
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi3 – 8Combined sources6
    Beta strandi11 – 19Combined sources9
    Beta strandi25 – 29Combined sources5
    Helixi36 – 38Combined sources3
    Helixi41 – 48Combined sources8
    Turni49 – 51Combined sources3
    Beta strandi53 – 57Combined sources5
    Turni70 – 72Combined sources3
    Helixi77 – 90Combined sources14
    Beta strandi94 – 101Combined sources8
    Helixi103 – 114Combined sources12
    Helixi116 – 118Combined sources3
    Beta strandi119 – 126Combined sources8
    Helixi134 – 143Combined sources10
    Helixi156 – 166Combined sources11
    Helixi172 – 187Combined sources16
    Beta strandi189 – 191Combined sources3
    Helixi195 – 208Combined sources14
    Turni209 – 211Combined sources3
    Helixi218 – 221Combined sources4
    Helixi227 – 235Combined sources9
    Beta strandi240 – 245Combined sources6
    Beta strandi249 – 251Combined sources3
    Helixi255 – 261Combined sources7
    Beta strandi266 – 271Combined sources6
    Helixi280 – 282Combined sources3
    Helixi283 – 296Combined sources14

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1Q0RX-ray1.45A1-298[»]
    1Q0ZX-ray1.95A1-298[»]
    ProteinModelPortaliQ54528.
    SMRiQ54528.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ54528.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini24 – 277AB hydrolase-1Sequence analysisAdd BLAST254

    Sequence similaritiesi

    Phylogenomic databases

    KOiK15957.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    [Graphical view]
    PfamiPF00561. Abhydrolase_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q54528-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSERIVPSGD VELWSDDFGD PADPALLLVM GGNLSALGWP DEFARRLADG
    60 70 80 90 100
    GLHVIRYDHR DTGRSTTRDF AAHPYGFGEL AADAVAVLDG WGVDRAHVVG
    110 120 130 140 150
    LSMGATITQV IALDHHDRLS SLTMLLGGGL DIDFDANIER VMRGEPTLDG
    160 170 180 190 200
    LPGPQQPFLD ALALMNQPAE GRAAEVAKRV SKWRILSGTG VPFDDAEYAR
    210 220 230 240 250
    WEERAIDHAG GVLAEPYAHY SLTLPPPSRA AELREVTVPT LVIQAEHDPI
    260 270 280 290
    APAPHGKHLA GLIPTARLAE IPGMGHALPS SVHGPLAEVI LAHTRSAA
    Length:298
    Mass (Da):31,792
    Last modified:November 1, 1996 - v1
    Checksum:i04FB4141C5A6E424
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U10405 Genomic DNA. Translation: AAA83422.1.
    PIRiA57139.

    Genome annotation databases

    KEGGiag:AAA83422.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U10405 Genomic DNA. Translation: AAA83422.1.
    PIRiA57139.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1Q0RX-ray1.45A1-298[»]
    1Q0ZX-ray1.95A1-298[»]
    ProteinModelPortaliQ54528.
    SMRiQ54528.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    ESTHERistrpu-rdmC. Aclacinomycin-methylesterase_RdmC.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    KEGGiag:AAA83422.

    Phylogenomic databases

    KOiK15957.

    Enzyme and pathway databases

    UniPathwayiUPA01043.
    BioCyciMetaCyc:MONOMER-18184.
    BRENDAi3.1.1.95. 6079.

    Miscellaneous databases

    EvolutionaryTraceiQ54528.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    [Graphical view]
    PfamiPF00561. Abhydrolase_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiRDMC_STREF
    AccessioniPrimary (citable) accession number: Q54528
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 19, 2014
    Last sequence update: November 1, 1996
    Last modified: November 2, 2016
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.