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Protein

Aclacinomycin methylesterase RdmC

Gene

rdmC

Organism
Streptomyces purpurascens
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the anthracycline aclacinomycin which is an aromatic polyketide antibiotic that exhibits high cytotoxicity and is widely applied in the chemotherapy of a variety of cancers. Catalyzes the removal of the methoxy group from the C-15 position of aclacinomycin T and A to yield 15-demethoxyaclacinomycin T and A, respectively.2 Publications

Catalytic activityi

Aclacinomycin T + H2O = 15-demethylaclacinomycin T + methanol.1 Publication

Kineticsi

  1. KM=15.5 µM for aclacinomycin T (at pH 7.5 and 37 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    Temperature dependencei

    Optimum temperature is 37 degrees Celsius. The activity at 30 and 45 degrees Celsius is 58% and 87% of the optimal activity, respectively. Exceptionally high temperature stability.1 Publication

    Pathwayi: aclacinomycin biosynthesis

    This protein is involved in the pathway aclacinomycin biosynthesis, which is part of Antibiotic biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway aclacinomycin biosynthesis and in Antibiotic biosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei102 – 1021Sequence analysis
    Active sitei248 – 2481Sequence analysis
    Active sitei276 – 2761Sequence analysis

    GO - Molecular functioni

    • carboxylic ester hydrolase activity Source: UniProtKB

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-18184.
    BRENDAi3.1.1.95. 6079.
    UniPathwayiUPA01043.

    Protein family/group databases

    ESTHERistrpu-rdmC. Aclacinomycin-methylesterase_RdmC.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aclacinomycin methylesterase RdmC (EC:3.1.1.95)
    Gene namesi
    Name:rdmC
    OrganismiStreptomyces purpurascens
    Taxonomic identifieri1924 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methionineiRemoved1 Publication
    Chaini2 – 298297Aclacinomycin methylesterase RdmCPRO_0000425719Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.2 Publications

    Structurei

    Secondary structure

    1
    298
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 86Combined sources
    Beta strandi11 – 199Combined sources
    Beta strandi25 – 295Combined sources
    Helixi36 – 383Combined sources
    Helixi41 – 488Combined sources
    Turni49 – 513Combined sources
    Beta strandi53 – 575Combined sources
    Turni70 – 723Combined sources
    Helixi77 – 9014Combined sources
    Beta strandi94 – 1018Combined sources
    Helixi103 – 11412Combined sources
    Helixi116 – 1183Combined sources
    Beta strandi119 – 1268Combined sources
    Helixi134 – 14310Combined sources
    Helixi156 – 16611Combined sources
    Helixi172 – 18716Combined sources
    Beta strandi189 – 1913Combined sources
    Helixi195 – 20814Combined sources
    Turni209 – 2113Combined sources
    Helixi218 – 2214Combined sources
    Helixi227 – 2359Combined sources
    Beta strandi240 – 2456Combined sources
    Beta strandi249 – 2513Combined sources
    Helixi255 – 2617Combined sources
    Beta strandi266 – 2716Combined sources
    Helixi280 – 2823Combined sources
    Helixi283 – 29614Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Q0RX-ray1.45A1-298[»]
    1Q0ZX-ray1.95A1-298[»]
    ProteinModelPortaliQ54528.
    SMRiQ54528. Positions 2-298.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ54528.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    KOiK15957.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    [Graphical view]
    PfamiPF00561. Abhydrolase_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q54528-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSERIVPSGD VELWSDDFGD PADPALLLVM GGNLSALGWP DEFARRLADG
    60 70 80 90 100
    GLHVIRYDHR DTGRSTTRDF AAHPYGFGEL AADAVAVLDG WGVDRAHVVG
    110 120 130 140 150
    LSMGATITQV IALDHHDRLS SLTMLLGGGL DIDFDANIER VMRGEPTLDG
    160 170 180 190 200
    LPGPQQPFLD ALALMNQPAE GRAAEVAKRV SKWRILSGTG VPFDDAEYAR
    210 220 230 240 250
    WEERAIDHAG GVLAEPYAHY SLTLPPPSRA AELREVTVPT LVIQAEHDPI
    260 270 280 290
    APAPHGKHLA GLIPTARLAE IPGMGHALPS SVHGPLAEVI LAHTRSAA
    Length:298
    Mass (Da):31,792
    Last modified:November 1, 1996 - v1
    Checksum:i04FB4141C5A6E424
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U10405 Genomic DNA. Translation: AAA83422.1.
    PIRiA57139.

    Genome annotation databases

    KEGGiag:AAA83422.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U10405 Genomic DNA. Translation: AAA83422.1.
    PIRiA57139.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Q0RX-ray1.45A1-298[»]
    1Q0ZX-ray1.95A1-298[»]
    ProteinModelPortaliQ54528.
    SMRiQ54528. Positions 2-298.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    ESTHERistrpu-rdmC. Aclacinomycin-methylesterase_RdmC.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    KEGGiag:AAA83422.

    Phylogenomic databases

    KOiK15957.

    Enzyme and pathway databases

    UniPathwayiUPA01043.
    BioCyciMetaCyc:MONOMER-18184.
    BRENDAi3.1.1.95. 6079.

    Miscellaneous databases

    EvolutionaryTraceiQ54528.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    [Graphical view]
    PfamiPF00561. Abhydrolase_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiRDMC_STREF
    AccessioniPrimary (citable) accession number: Q54528
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 19, 2014
    Last sequence update: November 1, 1996
    Last modified: September 7, 2016
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.