RdmB - Streptomyces purpurascens

Preferred order of sections

Names and origin

Protein names

Submitted name:
RdmB EMBL AAA83421.1

Gene names

Name:

rdmB EMBL AAA83421.1

Organism

Streptomyces purpurascens EMBL AAA83421.1

Taxonomic identifier

1924 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	S-adenosyl-L-methionine PDB 1XDS PDB 1QZZ
Technical term	3D-structure PDB 1XDS PDB 1R00 PDB 1QZZ PDB 1XDU
Gene Ontology (GO)
Molecular function	O-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Region

190 – 191

2

S-adenosyl-L-homocysteine binding PDB 1R00

Region

190 – 191

2

S-adenosyl-L-methionine binding PDB 1XDS PDB 1QZZ

Region

240 – 241

2

S-adenosyl-L-homocysteine binding PDB 1R00

Region

240 – 241

2

S-adenosyl-L-methionine binding PDB 1XDS PDB 1QZZ

Sites

Binding site

146

1

S-adenosyl-L-homocysteine PDB 1R00

Binding site

146

1

S-adenosyl-L-methionine PDB 1XDS PDB 1QZZ

Binding site

171

1

S-adenosyl-L-homocysteine PDB 1R00

Binding site

171

1

S-adenosyl-L-methionine PDB 1QZZ

Binding site

213

1

S-adenosyl-L-homocysteine PDB 1R00

Binding site

213

1

S-adenosyl-L-methionine PDB 1XDS PDB 1QZZ

Binding site

255

1

S-adenosyl-L-homocysteine PDB 1R00

Binding site

255

1

S-adenosyl-L-methionine PDB 1XDS PDB 1QZZ

Sequences

Sequence

Length

Mass (Da)

Tools

Q54527 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 427F37F5C59EEAD2
Show »

FASTA

374

39,797

        10         20         30         40         50         60 
MSSSSPGEPL EPTDQDLDVL LKNLGNLVTP MALRVAATLR LVDHLLAGAD TLAGLADRTD 

        70         80         90        100        110        120 
THPQALSRLV RHLTVVGVLE GGEKQGRPLR PTRLGMLLAD GHPAQQRAWL DLNGAVSHAD 

       130        140        150        160        170        180 
LAFTGLLDVV RTGRPAYAGR YGRPFWEDLS ADVALADSFD ALMSCDEDLA YEAPADAYDW 

       190        200        210        220        230        240 
SAVRHVLDVG GGNGGMLAAI ALRAPHLRGT LVELAGPAER ARRRFADAGL ADRVTVAEGD 

       250        260        270        280        290        300 
FFKPLPVTAD VVLLSFVLLN WSDEDALTIL RGCVRALEPG GRLLVLDRAD VEGDGADRFF 

       310        320        330        340        350        360 
STLLDLRMLT FMGGRVRTRD EVVDLAGSAG LALASERTSG STTLPFDFSI LEFTAVSEEA 

       370 
APAAQASEAL PAQE

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References

[1]	"Nucleotide sequences and expression of genes from Streptomyces purpurascens that cause the production of new anthracyclines in Streptomyces galilaeus." Niemi J., Mantsala P. J. Bacteriol. 177:2942-2945(1995) [PubMed: 7751313] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 25489 EMBL AAA83421.1.
[2]	"Hybrid anthracycline antibiotics: production of new anthracyclines by cloned genes from Streptomyces purpurascens in Streptomyces galilaeus." Niemi J., Ylihonko K., Hakala J., Parssinen R., Kopio A., Mantsala P. Microbiology 140:1351-1358(1994) [PubMed: 8081500] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 25489 EMBL AAA83421.1.
[3]	"Characterization of the gene cluster involved in rhodomycin biosynthesis." Halo L., Wang Y., Mantsala P., Hakala J., Ylihonko K. Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 25489 EMBL AAA83421.1.
[4]	Niemi J.T. Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 25489 EMBL AAA83421.1.
[5]	Halo L., Wang Y., Mantsala P., Hakala J., Ylihonko K. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 25489 EMBL AAA83421.1.
[6]	"Crystal structure of aclacinomycin-10-hydroxylase, a S-adenosyl-L-methionine-dependent methyltransferase homolog involved in anthracycline biosynthesis in Streptomyces purpurascens." Jansson A., Niemi J., Lindqvist Y., Mantsala P., Schneider G. J. Mol. Biol. 334:269-280(2003) [PubMed: 14607118] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE AND S-ADENOSYL-L-METHIONINE.
[7]	"Aclacinomycin 10-hydroxylase is a novel substrate-assisted hydroxylase requiring S-adenosyl-L-methionine as cofactor." Jansson A., Koskiniemi H., Erola A., Wang J., Mantsala P., Schneider G., Niemi J. J. Biol. Chem. 280:3636-3644(2005) [PubMed: 15548527] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-METHIONINE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U10405 Genomic DNA. Translation: AAA83421.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QZZ	X-ray	2.10	A	1-374	[»]
1R00	X-ray	2.50	A	1-374	[»]
1XDS	X-ray	2.30	A/B	1-374	[»]
1XDU	X-ray	2.70	A	1-374	[»]

ProteinModelPortal

Q54527.

SMR

Q54527. Positions 10-357.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR016461. O-MeTrfase_COMT_euk.
IPR001077. O_MeTrfase_2.
IPR011991. WHTH_trsnscrt_rep_DNA-bd.
[Graphical view]

Gene3D

G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.

Pfam

PF00891. Methyltransf_2. 1 hit.
[Graphical view]

PIRSF

PIRSF005739. O-mtase. 1 hit.

ProtoNet

Search...

Entry information

Entry name

Q54527_9ACTO

Accession

Primary (citable) accession number: Q54527

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	November 1, 1996
Last modified:	July 27, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)