Reviewed, UniProtKB/Swiss-Prot Q54520 (CPSD2_STRPN)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein kinase cpsD
    EC=2.7.10.2

Gene names

Name:	cpsD
Synonyms:	cps19fD

Organism

Streptococcus pneumoniae

Taxonomic identifier

1313 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the regulation of capsular polysaccharide biosynthesis. Autophosphorylation of cpsD attenuates its activity and reduces the level of encapsulation. May be part of a complex that directs the coordinated polymerization and export to the cell surface of the capsular polysaccharide.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Enzyme regulation	Dephosphorylated and activated by cpsB.
Pathway	Capsule biogenesis; capsule polysaccharide biosynthesis.
Subcellular location	Cytoplasm Probable.
Post-translational modification	Autophosphorylated.
Sequence similarities	Belongs to the cpsD/capB family.

Ontologies

Keywords
Biological process	Capsule biogenesis/degradation Exopolysaccharide synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	capsule polysaccharide biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW enzyme regulator activity Inferred from electronic annotation. Source: InterPro non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 227	227	Tyrosine-protein kinase cpsD		PRO_0000217240
Natural variations
	Natural variant	14	1	I → V in strain: NCTC 11906 and PO-329.
	Natural variant	74	1	T → I in strain: NCTC 11906 and PO-329.

Sequences

	Sequence		Length	Mass (Da)
	Q54520-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 42EF26DE49CF9773	FASTA	227	24,949
	10 20 30 40 50 60 MPTLEIAQKK LEFIKKAEEY YNALCTNIQL SGDKLKVISV TSVNPGEGKT TTSVNIARSF 70 80 90 100 110 120 ARAGYKTLLI DGDTRNSVMS GFFKSREKIT GLTEFLSGTA DLSHGLCDTN IENLFVVQSG 130 140 150 160 170 180 TVSPNPTALL QSKNFNDMIE TLRKYFDYII VDTAPIGIVI DAAIITQKCD ASILVTATGE 190 200 210 220 VNKRDVQKAK QQLEQTGKLF LGVVFNKLDI SVDKYGVYGF YGNYGKK

References

[1]	"Nucleotide sequence analysis of genes essential for capsular polysaccharide biosynthesis in Streptococcus pneumoniae type 19F." Guidolin A., Morona J.K., Morona R., Hansman D., Paton J.C. Infect. Immun. 62:5384-5396(1994) [PubMed: 7960118] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Serotype 19F.
[2]	"Recombinational exchanges at the capsular polysaccharide biosynthetic locus lead to frequent serotype changes among natural isolates of Streptococcus pneumoniae." Coffey T.J., Enright M.C., Daniels M., Morona J.K., Morona R., Hryniewicz W., Paton J.C., Spratt B.G. Mol. Microbiol. 27:73-83(1998) [PubMed: 9466257] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-196. Strain: NCTC 11906 / Serotype 19F, PO-329 / Serotype 19F, SP-496 / Serotype 19F and SP-GA71 / Serotype 19F.
[3]	"Tyrosine phosphorylation of CpsD negatively regulates capsular polysaccharide biosynthesis in Streptococcus pneumoniae." Morona J.K., Paton J.C., Miller D.C., Morona R. Mol. Microbiol. 35:1431-1442(2000) [PubMed: 10760144] [Abstract] Cited for: CHARACTERIZATION. Strain: Rx1-19F / Serotype 19F.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U09239 Genomic DNA. Translation: AAC44961.1. AF030367 Genomic DNA. Translation: AAC38719.1. AF030368 Genomic DNA. Translation: AAC38724.1. AF030370 Genomic DNA. Translation: AAC38733.1. AF030371 Genomic DNA. Translation: AAC38738.1.
3D structure databases
ModBase	Search...
PTM databases
PhosSite	Q54520.
Enzyme and pathway databases
BRENDA	2.7.10.2. 600.
Family and domain databases
InterPro	IPR005702. EPS_synthesis. [Graphical view]
TIGRFAMs	TIGR01007. eps_fam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CPSD2_STRPN

Accession

Primary (citable) accession number: Q54520
Secondary accession number(s): O52234

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2003
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)