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Protein

Tyrosine-protein kinase CpsD

Gene

cpsD

Organism
Streptococcus pneumoniae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the regulation of capsular polysaccharide biosynthesis. Autophosphorylation of CpsD attenuates its activity and reduces the level of encapsulation. May be part of a complex that directs the coordinated polymerization and export to the cell surface of the capsular polysaccharide.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme regulationi

Dephosphorylated and activated by CpsB.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. capsule polysaccharide biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Capsule biogenesis/degradation, Exopolysaccharide synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00934.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase CpsD (EC:2.7.10.2)
Gene namesi
Name:cpsD
Synonyms:cps19fD
OrganismiStreptococcus pneumoniae
Taxonomic identifieri1313 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227Tyrosine-protein kinase CpsDPRO_0000217240Add
BLAST

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

PTM databases

PhosSiteiP0411117.

Structurei

3D structure databases

ProteinModelPortaliQ54520.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CpsD/CapB family.Curated

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR025669. AAA_dom.
IPR005702. EPS_synthesis.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF13614. AAA_31. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01007. eps_fam. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54520-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPTLEIAQKK LEFIKKAEEY YNALCTNIQL SGDKLKVISV TSVNPGEGKT
60 70 80 90 100
TTSVNIARSF ARAGYKTLLI DGDTRNSVMS GFFKSREKIT GLTEFLSGTA
110 120 130 140 150
DLSHGLCDTN IENLFVVQSG TVSPNPTALL QSKNFNDMIE TLRKYFDYII
160 170 180 190 200
VDTAPIGIVI DAAIITQKCD ASILVTATGE VNKRDVQKAK QQLEQTGKLF
210 220
LGVVFNKLDI SVDKYGVYGF YGNYGKK
Length:227
Mass (Da):24,949
Last modified:November 1, 1996 - v1
Checksum:i42EF26DE49CF9773
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti14 – 141I → V in strain: NCTC 11906 and PO-329.
Natural varianti74 – 741T → I in strain: NCTC 11906 and PO-329.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09239 Genomic DNA. Translation: AAC44961.1.
AF030367 Genomic DNA. Translation: AAC38719.1.
AF030368 Genomic DNA. Translation: AAC38724.1.
AF030370 Genomic DNA. Translation: AAC38733.1.
AF030371 Genomic DNA. Translation: AAC38738.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09239 Genomic DNA. Translation: AAC44961.1.
AF030367 Genomic DNA. Translation: AAC38719.1.
AF030368 Genomic DNA. Translation: AAC38724.1.
AF030370 Genomic DNA. Translation: AAC38733.1.
AF030371 Genomic DNA. Translation: AAC38738.1.

3D structure databases

ProteinModelPortaliQ54520.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosSiteiP0411117.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00934.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR025669. AAA_dom.
IPR005702. EPS_synthesis.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF13614. AAA_31. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01007. eps_fam. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence analysis of genes essential for capsular polysaccharide biosynthesis in Streptococcus pneumoniae type 19F."
    Guidolin A., Morona J.K., Morona R., Hansman D., Paton J.C.
    Infect. Immun. 62:5384-5396(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Serotype 19F.
  2. "Recombinational exchanges at the capsular polysaccharide biosynthetic locus lead to frequent serotype changes among natural isolates of Streptococcus pneumoniae."
    Coffey T.J., Enright M.C., Daniels M., Morona J.K., Morona R., Hryniewicz W., Paton J.C., Spratt B.G.
    Mol. Microbiol. 27:73-83(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-196.
    Strain: NCTC 11906 / Serotype 19F, PO-329 / Serotype 19F, SP-496 / Serotype 19F and SP-GA71 / Serotype 19F.
  3. "Tyrosine phosphorylation of CpsD negatively regulates capsular polysaccharide biosynthesis in Streptococcus pneumoniae."
    Morona J.K., Paton J.C., Miller D.C., Morona R.
    Mol. Microbiol. 35:1431-1442(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: Rx1-19F / Serotype 19F.

Entry informationi

Entry nameiCPSD_STREE
AccessioniPrimary (citable) accession number: Q54520
Secondary accession number(s): O52234
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.