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Q54520

- CPSD_STREE

UniProt

Q54520 - CPSD_STREE

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Protein

Tyrosine-protein kinase CpsD

Gene
cpsD, cps19fD
Organism
Streptococcus pneumoniae
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the regulation of capsular polysaccharide biosynthesis. Autophosphorylation of CpsD attenuates its activity and reduces the level of encapsulation. May be part of a complex that directs the coordinated polymerization and export to the cell surface of the capsular polysaccharide.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme regulationi

Dephosphorylated and activated by CpsB.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. enzyme regulator activity Source: InterPro
  3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. capsule polysaccharide biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Capsule biogenesis/degradation, Exopolysaccharide synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00934.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase CpsD (EC:2.7.10.2)
Gene namesi
Name:cpsD
Synonyms:cps19fD
OrganismiStreptococcus pneumoniae
Taxonomic identifieri1313 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

Cytoplasm Inferred

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227Tyrosine-protein kinase CpsDPRO_0000217240Add
BLAST

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

PTM databases

PhosSiteiP0411117.

Structurei

3D structure databases

ProteinModelPortaliQ54520.

Family & Domainsi

Sequence similaritiesi

Belongs to the CpsD/CapB family.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR025669. AAA_dom.
IPR005702. EPS_synthesis.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF13614. AAA_31. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01007. eps_fam. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54520-1 [UniParc]FASTAAdd to Basket

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MPTLEIAQKK LEFIKKAEEY YNALCTNIQL SGDKLKVISV TSVNPGEGKT    50
TTSVNIARSF ARAGYKTLLI DGDTRNSVMS GFFKSREKIT GLTEFLSGTA 100
DLSHGLCDTN IENLFVVQSG TVSPNPTALL QSKNFNDMIE TLRKYFDYII 150
VDTAPIGIVI DAAIITQKCD ASILVTATGE VNKRDVQKAK QQLEQTGKLF 200
LGVVFNKLDI SVDKYGVYGF YGNYGKK 227
Length:227
Mass (Da):24,949
Last modified:November 1, 1996 - v1
Checksum:i42EF26DE49CF9773
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti14 – 141I → V in strain: NCTC 11906 and PO-329.
Natural varianti74 – 741T → I in strain: NCTC 11906 and PO-329.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09239 Genomic DNA. Translation: AAC44961.1.
AF030367 Genomic DNA. Translation: AAC38719.1.
AF030368 Genomic DNA. Translation: AAC38724.1.
AF030370 Genomic DNA. Translation: AAC38733.1.
AF030371 Genomic DNA. Translation: AAC38738.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09239 Genomic DNA. Translation: AAC44961.1 .
AF030367 Genomic DNA. Translation: AAC38719.1 .
AF030368 Genomic DNA. Translation: AAC38724.1 .
AF030370 Genomic DNA. Translation: AAC38733.1 .
AF030371 Genomic DNA. Translation: AAC38738.1 .

3D structure databases

ProteinModelPortali Q54520.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosSitei P0411117.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00934 .

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR025669. AAA_dom.
IPR005702. EPS_synthesis.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF13614. AAA_31. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR01007. eps_fam. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence analysis of genes essential for capsular polysaccharide biosynthesis in Streptococcus pneumoniae type 19F."
    Guidolin A., Morona J.K., Morona R., Hansman D., Paton J.C.
    Infect. Immun. 62:5384-5396(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Serotype 19F.
  2. "Recombinational exchanges at the capsular polysaccharide biosynthetic locus lead to frequent serotype changes among natural isolates of Streptococcus pneumoniae."
    Coffey T.J., Enright M.C., Daniels M., Morona J.K., Morona R., Hryniewicz W., Paton J.C., Spratt B.G.
    Mol. Microbiol. 27:73-83(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-196.
    Strain: NCTC 11906 / Serotype 19F, PO-329 / Serotype 19F, SP-496 / Serotype 19F and SP-GA71 / Serotype 19F.
  3. "Tyrosine phosphorylation of CpsD negatively regulates capsular polysaccharide biosynthesis in Streptococcus pneumoniae."
    Morona J.K., Paton J.C., Miller D.C., Morona R.
    Mol. Microbiol. 35:1431-1442(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: Rx1-19F / Serotype 19F.

Entry informationi

Entry nameiCPSD_STREE
AccessioniPrimary (citable) accession number: Q54520
Secondary accession number(s): O52234
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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