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Q54520 (CPSD_STREE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein kinase CpsD

EC=2.7.10.2
Gene names
Name:cpsD
Synonyms:cps19fD
OrganismStreptococcus pneumoniae
Taxonomic identifier1313 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the regulation of capsular polysaccharide biosynthesis. Autophosphorylation of CpsD attenuates its activity and reduces the level of encapsulation. May be part of a complex that directs the coordinated polymerization and export to the cell surface of the capsular polysaccharide.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme regulation

Dephosphorylated and activated by CpsB.

Pathway

Capsule biogenesis; capsule polysaccharide biosynthesis.

Subcellular location

Cytoplasm Probable.

Post-translational modification

Autophosphorylated.

Sequence similarities

Belongs to the CpsD/CapB family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 227227Tyrosine-protein kinase CpsD
PRO_0000217240

Natural variations

Natural variant141I → V in strain: NCTC 11906 and PO-329.
Natural variant741T → I in strain: NCTC 11906 and PO-329.

Sequences

Sequence LengthMass (Da)Tools
Q54520 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 42EF26DE49CF9773

FASTA22724,949
        10         20         30         40         50         60 
MPTLEIAQKK LEFIKKAEEY YNALCTNIQL SGDKLKVISV TSVNPGEGKT TTSVNIARSF 

        70         80         90        100        110        120 
ARAGYKTLLI DGDTRNSVMS GFFKSREKIT GLTEFLSGTA DLSHGLCDTN IENLFVVQSG 

       130        140        150        160        170        180 
TVSPNPTALL QSKNFNDMIE TLRKYFDYII VDTAPIGIVI DAAIITQKCD ASILVTATGE 

       190        200        210        220 
VNKRDVQKAK QQLEQTGKLF LGVVFNKLDI SVDKYGVYGF YGNYGKK 

« Hide

References

[1]"Nucleotide sequence analysis of genes essential for capsular polysaccharide biosynthesis in Streptococcus pneumoniae type 19F."
Guidolin A., Morona J.K., Morona R., Hansman D., Paton J.C.
Infect. Immun. 62:5384-5396(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Serotype 19F.
[2]"Recombinational exchanges at the capsular polysaccharide biosynthetic locus lead to frequent serotype changes among natural isolates of Streptococcus pneumoniae."
Coffey T.J., Enright M.C., Daniels M., Morona J.K., Morona R., Hryniewicz W., Paton J.C., Spratt B.G.
Mol. Microbiol. 27:73-83(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-196.
Strain: NCTC 11906 / Serotype 19F, PO-329 / Serotype 19F, SP-496 / Serotype 19F and SP-GA71 / Serotype 19F.
[3]"Tyrosine phosphorylation of CpsD negatively regulates capsular polysaccharide biosynthesis in Streptococcus pneumoniae."
Morona J.K., Paton J.C., Miller D.C., Morona R.
Mol. Microbiol. 35:1431-1442(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: Rx1-19F / Serotype 19F.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U09239 Genomic DNA. Translation: AAC44961.1.
AF030367 Genomic DNA. Translation: AAC38719.1.
AF030368 Genomic DNA. Translation: AAC38724.1.
AF030370 Genomic DNA. Translation: AAC38733.1.
AF030371 Genomic DNA. Translation: AAC38738.1.

3D structure databases

ProteinModelPortalQ54520.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosSiteP0411117.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00934.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR025669. AAA_dom.
IPR005702. EPS_synthesis.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF13614. AAA_31. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR01007. eps_fam. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCPSD_STREE
AccessionPrimary (citable) accession number: Q54520
Secondary accession number(s): O52234
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways