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Q54518 (CPSB_STREE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein phosphatase CpsB

EC=3.1.3.48
Gene names
Name:cpsB
Synonyms:cap1B, cps19fB
OrganismStreptococcus pneumoniae
Taxonomic identifier1313 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length243 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Dephosphorylates CpsD. Involved in the regulation of capsular polysaccharide biosynthesis.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Cofactor

Manganese.

Pathway

Capsule biogenesis; capsule polysaccharide biosynthesis.

Sequence similarities

Belongs to the CpsB/CapC family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 243243Tyrosine-protein phosphatase CpsB
PRO_0000057892

Natural variations

Natural variant2261A → V in strain: NCTC 11906.

Experimental info

Mutagenesis1991D → N: Loss of activity; when associated with Q-201. Ref.5
Mutagenesis2011H → Q: Loss of activity; when associated with N-199. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q54518 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 810A4C802EC43079

FASTA24328,354
        10         20         30         40         50         60 
MIDIHSHIVF DVDDGPKSRE ESKALLAESY RQGVRTIVST SHRRKGMFET PEEKIAENFL 

        70         80         90        100        110        120 
QVREIAKEVA DDLVIAYGAE IYYTLDALEK LEKKEIPTLN DSRYALIEFS MHTSYRQIHT 

       130        140        150        160        170        180 
GLSNILMLGI TPVIAHIERY DALENNEKRV RELIDMGCYT QINSYHVSKP KFFGEKYKFM 

       190        200        210        220        230        240 
KKRARYFLER DLVHVVASDM HNLDSRPPYM QQAYDIIAKK YGAKKAKELF VDNPRKIIMD 


QLI 

« Hide

References

[1]"Nucleotide sequence analysis of genes essential for capsular polysaccharide biosynthesis in Streptococcus pneumoniae type 19F."
Guidolin A., Morona J.K., Morona R., Hansman D., Paton J.C.
Infect. Immun. 62:5384-5396(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Serotype 19F.
[2]"Molecular organization of the genes required for the synthesis of type 1 capsular polysaccharide of Streptococcus pneumoniae: formation of binary encapsulated pneumococci and identification of cryptic dTDP-rhamnose biosynthesis genes."
Munoz R., Mollerach M.E., Lopez R., Garcia E.
Mol. Microbiol. 25:79-92(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Recombinational exchanges at the capsular polysaccharide biosynthetic locus lead to frequent serotype changes among natural isolates of Streptococcus pneumoniae."
Coffey T.J., Enright M.C., Daniels M., Morona J.K., Morona R., Hryniewicz W., Paton J.C., Spratt B.G.
Mol. Microbiol. 27:73-83(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: NCTC 11906 / Serotype 19F, PO-329 / Serotype 19F, SP-496 / Serotype 19F, SP-GA71 / Serotype 19F, SP-VA92 / Serotype 19F and SP-VA96 / Serotype 19F.
[4]"Analysis of capsule loci from various Streptococcus pneumoniae serotypes using long-range PCR identifies two classes of cpsC."
Morona J.K., Morona R., Paton J.C.
Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 220-243.
Strain: Serotype 19B.
[5]"Streptococcus pneumoniae capsule biosynthesis protein CpsB is a novel manganese-dependent phosphotyrosine-protein phosphatase."
Morona J.K., Morona R., Miller D.C., Paton J.C.
J. Bacteriol. 184:577-583(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, MUTAGENESIS OF ASP-199 AND HIS-201.
Strain: Rx1-19F / Serotype 19F.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U09239 Genomic DNA. Translation: AAC44959.1.
Z83335 Genomic DNA. Translation: CAB05935.1.
AF030367 Genomic DNA. Translation: AAC38717.1.
AF030368 Genomic DNA. Translation: AAC38722.1.
AF030369 Genomic DNA. Translation: AAC38727.1.
AF030370 Genomic DNA. Translation: AAC38731.1.
AF030371 Genomic DNA. Translation: AAC38736.1.
AF030372 Genomic DNA. Translation: AAC38741.1.
AF106137 Genomic DNA. Translation: AAD17985.1.

3D structure databases

ProteinModelPortalQ54518.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

PptaseDBP3D0411155.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00934.

Family and domain databases

InterProIPR016667. Caps_polysacc_synth_CpsB/CapC.
IPR004013. PHP_C.
[Graphical view]
PfamPF02811. PHP. 1 hit.
[Graphical view]
PIRSFPIRSF016557. Caps_synth_CpsB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCPSB_STREE
AccessionPrimary (citable) accession number: Q54518
Secondary accession number(s): O08049, O08278, O52232
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways