Q54518 (CPSB_STREE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase CpsB
EC=3.1.3.48

Gene names

Name:	cpsB
Synonyms:	cap1B, cps19fB

Organism

Streptococcus pneumoniae

Taxonomic identifier

1313 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Dephosphorylates CpsD. Involved in the regulation of capsular polysaccharide biosynthesis.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Cofactor	Manganese.
Pathway	Capsule biogenesis; capsule polysaccharide biosynthesis.
Sequence similarities	Belongs to the CpsB/CapC family.

Ontologies

Keywords
Biological process	Capsule biogenesis/degradation Exopolysaccharide synthesis
Ligand	Manganese
Molecular function	Hydrolase Protein phosphatase
Gene Ontology (GO)
Biological_process	capsule polysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Molecular_function	manganese ion binding Inferred from electronic annotation. Source: InterPro protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	Tyrosine-protein phosphatase CpsB		PRO_0000057892
Natural variations
	Natural variant	226	1	A → V in strain: NCTC 11906.
Experimental info
	Mutagenesis	199	1	D → N: Loss of activity; when associated with Q-201. Ref.5
	Mutagenesis	201	1	H → Q: Loss of activity; when associated with N-199. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q54518 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 810A4C802EC43079	FASTA	243	28,354
	10 20 30 40 50 60 MIDIHSHIVF DVDDGPKSRE ESKALLAESY RQGVRTIVST SHRRKGMFET PEEKIAENFL 70 80 90 100 110 120 QVREIAKEVA DDLVIAYGAE IYYTLDALEK LEKKEIPTLN DSRYALIEFS MHTSYRQIHT 130 140 150 160 170 180 GLSNILMLGI TPVIAHIERY DALENNEKRV RELIDMGCYT QINSYHVSKP KFFGEKYKFM 190 200 210 220 230 240 KKRARYFLER DLVHVVASDM HNLDSRPPYM QQAYDIIAKK YGAKKAKELF VDNPRKIIMD QLI

References

[1]	"Nucleotide sequence analysis of genes essential for capsular polysaccharide biosynthesis in Streptococcus pneumoniae type 19F." Guidolin A., Morona J.K., Morona R., Hansman D., Paton J.C. Infect. Immun. 62:5384-5396(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Serotype 19F.
[2]	"Molecular organization of the genes required for the synthesis of type 1 capsular polysaccharide of Streptococcus pneumoniae: formation of binary encapsulated pneumococci and identification of cryptic dTDP-rhamnose biosynthesis genes." Munoz R., Mollerach M.E., Lopez R., Garcia E. Mol. Microbiol. 25:79-92(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Recombinational exchanges at the capsular polysaccharide biosynthetic locus lead to frequent serotype changes among natural isolates of Streptococcus pneumoniae." Coffey T.J., Enright M.C., Daniels M., Morona J.K., Morona R., Hryniewicz W., Paton J.C., Spratt B.G. Mol. Microbiol. 27:73-83(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCTC 11906 / Serotype 19F, PO-329 / Serotype 19F, SP-496 / Serotype 19F, SP-GA71 / Serotype 19F, SP-VA92 / Serotype 19F and SP-VA96 / Serotype 19F.
[4]	"Analysis of capsule loci from various Streptococcus pneumoniae serotypes using long-range PCR identifies two classes of cpsC." Morona J.K., Morona R., Paton J.C. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 220-243. Strain: Serotype 19B.
[5]	"Streptococcus pneumoniae capsule biosynthesis protein CpsB is a novel manganese-dependent phosphotyrosine-protein phosphatase." Morona J.K., Morona R., Miller D.C., Paton J.C. J. Bacteriol. 184:577-583(2002) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, MUTAGENESIS OF ASP-199 AND HIS-201. Strain: Rx1-19F / Serotype 19F.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U09239 Genomic DNA. Translation: AAC44959.1. Z83335 Genomic DNA. Translation: CAB05935.1. AF030367 Genomic DNA. Translation: AAC38717.1. AF030368 Genomic DNA. Translation: AAC38722.1. AF030369 Genomic DNA. Translation: AAC38727.1. AF030370 Genomic DNA. Translation: AAC38731.1. AF030371 Genomic DNA. Translation: AAC38736.1. AF030372 Genomic DNA. Translation: AAC38741.1. AF106137 Genomic DNA. Translation: AAD17985.1.
3D structure databases
ProteinModelPortal	Q54518.
ModBase	Search...
Protein family/group databases
PptaseDB	P3D0411155.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00934.
Family and domain databases
InterPro	IPR016667. Caps_polysacc_synth_CpsB/CapC. IPR004013. PHP_C. [Graphical view]
Pfam	PF02811. PHP. 1 hit. [Graphical view]
PIRSF	PIRSF016557. Caps_synth_CpsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CPSB_STREE

Accession

Primary (citable) accession number: Q54518
Secondary accession number(s): O08049, O08278, O52232

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2003
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program