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Protein

DNA-(apurinic or apyrimidinic site) lyase

Gene

Apex1

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Probably binds two magnesium or manganese ions per subunit.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotation, EndonucleaseUniRule annotationImported, Hydrolase, LyaseUniRule annotation, Nuclease
Biological processDNA damage, DNA repairUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation (EC:4.2.99.18UniRule annotation)
Gene namesi
Name:Apex1Imported
ORF Names:mCG_16516Imported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus

Organism-specific databases

MGIiMGI:88042 Apex1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

CytoplasmUniRule annotation, MitochondrionUniRule annotation, NucleusUniRule annotation

PTM / Processingi

Proteomic databases

MaxQBiQ544Z7
PRIDEiQ544Z7

Expressioni

Gene expression databases

BgeeiENSMUSG00000035960
ExpressionAtlasiQ544Z7 baseline and differential

Interactioni

GO - Molecular functioni

Structurei

3D structure databases

SMRiQ544Z7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 308Endo/exonuclease/phosphataseInterPro annotationAdd BLAST244

Sequence similaritiesi

Belongs to the DNA repair enzymes AP/ExoA family.UniRule annotation

Phylogenomic databases

eggNOGiKOG1294 Eukaryota
COG0708 LUCA
HOVERGENiHBG050531
KOiK10771
OMAiGTAVFTK
OrthoDBiEOG091G0FDG

Family and domain databases

Gene3Di3.60.10.10, 1 hit
InterProiView protein in InterPro
IPR004808 AP_endonuc_1
IPR020847 AP_endonuclease_F1_BS
IPR020848 AP_endonuclease_F1_CS
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
PANTHERiPTHR22748 PTHR22748, 1 hit
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
SUPFAMiSSF56219 SSF56219, 1 hit
TIGRFAMsiTIGR00633 xth, 1 hit
PROSITEiView protein in PROSITE
PS00726 AP_NUCLEASE_F1_1, 1 hit
PS00727 AP_NUCLEASE_F1_2, 1 hit
PS00728 AP_NUCLEASE_F1_3, 1 hit
PS51435 AP_NUCLEASE_F1_4, 1 hit

Sequencei

Sequence statusi: Complete.

Q544Z7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKRGKKAAA DDGEEPKSEP ETKKSKGAAK KTEKEAAGEG PVLYEDPPDQ
60 70 80 90 100
KTSPSGKSAT LKICSWNVDG LRAWIKKKGL DWVKEEAPDI LCLQETKCSE
110 120 130 140 150
NKLPAELQEL PGLTHQYWSA PSDKEGYSGV GLLSRQCPLK VSYGIGEEEH
160 170 180 190 200
DQEGRVIVAE FESFVLVTAY VPNAGRGLVR LEYRQRWDEA FRKFLKDLAS
210 220 230 240 250
RKPLVLCGDL NVAHEEIDLR NPKGNKKNAG FTPQERQGFG ELLQAVPLAD
260 270 280 290 300
SFRHLYPNTA YAYTFWTYMM NARSKNVGWR LDYFLLSHSL LPALCDSKIR
310
SKALGSDHCP ITLYLAL
Length:317
Mass (Da):35,490
Last modified:May 24, 2005 - v1
Checksum:iCF086691FAC89C4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013296 mRNA Translation: BAB28774.1
CH466605 Genomic DNA Translation: EDL20835.1
RefSeqiNP_033817.1, NM_009687.2
UniGeneiMm.203
Mm.239117

Genome annotation databases

GeneIDi11792
KEGGimmu:11792

Similar proteinsi

Entry informationi

Entry nameiQ544Z7_MOUSE
AccessioniPrimary (citable) accession number: Q544Z7
Entry historyiIntegrated into UniProtKB/TrEMBL: May 24, 2005
Last sequence update: May 24, 2005
Last modified: June 20, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

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