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Protein

DNA-(apurinic or apyrimidinic site) lyase

Gene

Apex1

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Probably binds two magnesium or manganese ions per subunit.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotation, EndonucleaseUniRule annotationImported, Hydrolase, LyaseUniRule annotation, Nuclease
Biological processDNA damage, DNA repairUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation (EC:4.2.99.18UniRule annotation)
Gene namesi
Name:Apex1Imported
ORF Names:mCG_16516Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus

Organism-specific databases

MGIiMGI:88042. Apex1.

Subcellular locationi

  • Nucleus UniRule annotation
  • Cytoplasm UniRule annotation
  • Mitochondrion UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation, MitochondrionUniRule annotation, NucleusUniRule annotation

PTM / Processingi

Proteomic databases

MaxQBiQ544Z7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000035960.
ExpressionAtlasiQ544Z7. baseline and differential.

Interactioni

GO - Molecular functioni

Structurei

3D structure databases

SMRiQ544Z7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 308Endo/exonuclease/phosphataseInterPro annotationAdd BLAST244

Sequence similaritiesi

Belongs to the DNA repair enzymes AP/ExoA family.UniRule annotation

Phylogenomic databases

eggNOGiKOG1294. Eukaryota.
COG0708. LUCA.
HOVERGENiHBG050531.
KOiK10771.
OMAiGTAVFTK.
OrthoDBiEOG091G0FDG.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiView protein in InterPro
IPR004808. AP_endonuc_1.
IPR020847. AP_endonuclease_F1_BS.
IPR020848. AP_endonuclease_F1_CS.
IPR005135. Endo/exonuclease/phosphatase.
PANTHERiPTHR22748. PTHR22748. 1 hit.
PfamiView protein in Pfam
PF03372. Exo_endo_phos. 1 hit.
SUPFAMiSSF56219. SSF56219. 1 hit.
TIGRFAMsiTIGR00633. xth. 1 hit.
PROSITEiView protein in PROSITE
PS00726. AP_NUCLEASE_F1_1. 1 hit.
PS00727. AP_NUCLEASE_F1_2. 1 hit.
PS00728. AP_NUCLEASE_F1_3. 1 hit.
PS51435. AP_NUCLEASE_F1_4. 1 hit.

Sequencei

Sequence statusi: Complete.

Q544Z7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKRGKKAAA DDGEEPKSEP ETKKSKGAAK KTEKEAAGEG PVLYEDPPDQ
60 70 80 90 100
KTSPSGKSAT LKICSWNVDG LRAWIKKKGL DWVKEEAPDI LCLQETKCSE
110 120 130 140 150
NKLPAELQEL PGLTHQYWSA PSDKEGYSGV GLLSRQCPLK VSYGIGEEEH
160 170 180 190 200
DQEGRVIVAE FESFVLVTAY VPNAGRGLVR LEYRQRWDEA FRKFLKDLAS
210 220 230 240 250
RKPLVLCGDL NVAHEEIDLR NPKGNKKNAG FTPQERQGFG ELLQAVPLAD
260 270 280 290 300
SFRHLYPNTA YAYTFWTYMM NARSKNVGWR LDYFLLSHSL LPALCDSKIR
310
SKALGSDHCP ITLYLAL
Length:317
Mass (Da):35,490
Last modified:May 24, 2005 - v1
Checksum:iCF086691FAC89C4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013296 mRNA. Translation: BAB28774.1.
CH466605 Genomic DNA. Translation: EDL20835.1.
RefSeqiNP_033817.1. NM_009687.2.
UniGeneiMm.203.
Mm.239117.

Genome annotation databases

GeneIDi11792.
KEGGimmu:11792.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiQ544Z7_MOUSE
AccessioniPrimary (citable) accession number: Q544Z7
Entry historyiIntegrated into UniProtKB/TrEMBL: May 24, 2005
Last sequence update: May 24, 2005
Last modified: July 5, 2017
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)