ID   Q544Y7_MOUSE            Unreviewed;       166 AA.
AC   Q544Y7;
DT   24-MAY-2005, integrated into UniProtKB/TrEMBL.
DT   24-MAY-2005, sequence version 1.
DT   27-MAR-2024, entry version 153.
DE   RecName: Full=Cofilin-1 {ECO:0000256|ARBA:ARBA00015284};
DE   AltName: Full=Cofilin, non-muscle isoform {ECO:0000256|ARBA:ARBA00030239};
GN   Name=Cfl1 {ECO:0000313|EMBL:AAH94357.1, ECO:0000313|MGI:MGI:101757};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090 {ECO:0000313|EMBL:BAB29074.1};
RN   [1] {ECO:0000313|EMBL:BAB29074.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=BALB/C {ECO:0000313|EMBL:BAE39825.1}, C57BL/6J
RC   {ECO:0000313|EMBL:BAB29074.1}, and NOD {ECO:0000313|EMBL:BAC40467.1};
RC   TISSUE=Head {ECO:0000313|EMBL:BAB29074.1}, and Thymus
RC   {ECO:0000313|EMBL:BAC34363.1};
RX   PubMed=10349636; DOI=10.1016/S0076-6879(99)03004-9;
RA   Carninci P., Hayashizaki Y.;
RT   "High-efficiency full-length cDNA cloning.";
RL   Methods Enzymol. 303:19-44(1999).
RN   [2] {ECO:0000313|EMBL:BAB29074.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=BALB/C {ECO:0000313|EMBL:BAE39825.1}, C57BL/6J
RC   {ECO:0000313|EMBL:BAB29074.1}, and NOD {ECO:0000313|EMBL:BAC40467.1};
RC   TISSUE=Head {ECO:0000313|EMBL:BAB29074.1}, and Thymus
RC   {ECO:0000313|EMBL:BAC34363.1};
RX   PubMed=11042159; DOI=10.1101/gr.145100;
RA   Carninci P., Shibata Y., Hayatsu N., Sugahara Y., Shibata K., Itoh M.,
RA   Konno H., Okazaki Y., Muramatsu M., Hayashizaki Y.;
RT   "Normalization and subtraction of cap-trapper-selected cDNAs to prepare
RT   full-length cDNA libraries for rapid discovery of new genes.";
RL   Genome Res. 10:1617-1630(2000).
RN   [3] {ECO:0000313|EMBL:BAB29074.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=BALB/C {ECO:0000313|EMBL:BAE39825.1}, C57BL/6J
RC   {ECO:0000313|EMBL:BAB29074.1}, and NOD {ECO:0000313|EMBL:BAC40467.1};
RC   TISSUE=Head {ECO:0000313|EMBL:BAB29074.1}, and Thymus
RC   {ECO:0000313|EMBL:BAC34363.1};
RX   PubMed=11076861; DOI=10.1101/gr.152600;
RA   Shibata K., Itoh M., Aizawa K., Nagaoka S., Sasaki N., Carninci P.,
RA   Konno H., Akiyama J., Nishi K., Kitsunai T., Tashiro H., Itoh M., Sumi N.,
RA   Ishii Y., Nakamura S., Hazama M., Nishine T., Harada A., Yamamoto R.,
RA   Matsumoto H., Sakaguchi S., Ikegami T., Kashiwagi K., Fujiwake S.,
RA   Inoue K., Togawa Y., Izawa M., Ohara E., Watahiki M., Yoneda Y.,
RA   Ishikawa T., Ozawa K., Tanaka T., Matsuura S., Kawai J., Okazaki Y.,
RA   Muramatsu M., Inoue Y., Kira A., Hayashizaki Y.;
RT   "RIKEN integrated sequence analysis (RISA) system--384-format sequencing
RT   pipeline with 384 multicapillary sequencer.";
RL   Genome Res. 10:1757-1771(2000).
RN   [4] {ECO:0000313|EMBL:BAB29074.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAB29074.1};
RC   TISSUE=Head {ECO:0000313|EMBL:BAB29074.1};
RA   Adachi J., Aizawa K., Akahira S., Akimura T., Arai A., Aono H., Arakawa T.,
RA   Bono H., Carninci P., Fukuda S., Fukunishi Y., Furuno M., Hanagaki T.,
RA   Hara A., Hayatsu N., Hiramoto K., Hiraoka T., Hori F., Imotani K.,
RA   Ishii Y., Itoh M., Izawa M., Kasukawa T., Kato H., Kawai J., Kojima Y.,
RA   Konno H., Kouda M., Koya S., Kurihara C., Matsuyama T., Miyazaki A.,
RA   Nishi K., Nomura K., Numazaki R., Ohno M., Okazaki Y., Okido T., Owa C.,
RA   Saito H., Saito R., Sakai C., Sakai K., Sano H., Sasaki D., Shibata K.,
RA   Shibata Y., Shinagawa A., Shiraki T., Sogabe Y., Suzuki H., Tagami M.,
RA   Tagawa A., Takahashi F., Tanaka T., Tejima Y., Toya T., Yamamura T.,
RA   Yasunishi A., Yoshida K., Yoshino M., Muramatsu M., Hayashizaki Y.;
RL   Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
RN   [5] {ECO:0000313|EMBL:BAB29074.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=BALB/C {ECO:0000313|EMBL:BAE39825.1}, C57BL/6J
RC   {ECO:0000313|EMBL:BAB29074.1}, and NOD {ECO:0000313|EMBL:BAC40467.1};
RC   TISSUE=Head {ECO:0000313|EMBL:BAB29074.1}, and Thymus
RC   {ECO:0000313|EMBL:BAC34363.1};
RX   PubMed=11217851; DOI=10.1038/35055500;
RG   The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium;
RT   "Functional annotation of a full-length mouse cDNA collection.";
RL   Nature 409:685-690(2001).
RN   [6] {ECO:0000313|EMBL:BAC34363.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAC34363.1}, and NOD
RC   {ECO:0000313|EMBL:BAC40467.1};
RC   TISSUE=Thymus {ECO:0000313|EMBL:BAC34363.1};
RA   Adachi J., Aizawa K., Akimura T., Arakawa T., Bono H., Carninci P.,
RA   Fukuda S., Furuno M., Hanagaki T., Hara A., Hashizume W., Hayashida K.,
RA   Hayatsu N., Hiramoto K., Hiraoka T., Hirozane T., Hori F., Imotani K.,
RA   Ishii Y., Itoh M., Kagawa I., Kasukawa T., Katoh H., Kawai J., Kojima Y.,
RA   Kondo S., Konno H., Kouda M., Koya S., Kurihara C., Matsuyama T.,
RA   Miyazaki A., Murata M., Nakamura M., Nishi K., Nomura K., Numazaki R.,
RA   Ohno M., Ohsato N., Okazaki Y., Saito R., Saitoh H., Sakai C., Sakai K.,
RA   Sakazume N., Sano H., Sasaki D., Shibata K., Shinagawa A., Shiraki T.,
RA   Sogabe Y., Tagami M., Tagawa A., Takahashi F., Takaku-Akahira S.,
RA   Takeda Y., Tanaka T., Tomaru A., Toya T., Yasunishi A., Muramatsu M.,
RA   Hayashizaki Y.;
RL   Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN   [7] {ECO:0000313|EMBL:BAB29074.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=BALB/C {ECO:0000313|EMBL:BAE39825.1}, C57BL/6J
RC   {ECO:0000313|EMBL:BAB29074.1}, and NOD {ECO:0000313|EMBL:BAC40467.1};
RC   TISSUE=Head {ECO:0000313|EMBL:BAB29074.1}, and Thymus
RC   {ECO:0000313|EMBL:BAC34363.1};
RX   PubMed=12466851; DOI=10.1038/nature01266;
RG   The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team;
RT   "Analysis of the mouse transcriptome based on functional annotation of
RT   60,770 full-length cDNAs.";
RL   Nature 420:563-573(2002).
RN   [8] {ECO:0000313|EMBL:AAH94357.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6 {ECO:0000313|EMBL:AAH94357.1};
RC   TISSUE=Brain {ECO:0000313|EMBL:AAH94357.1};
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RA   Gerhard D.S., Wagner L., Feingold E.A., Shenmen C.M., Grouse L.H.,
RA   Schuler G., Klein S.L., Old S., Rasooly R., Good P., Guyer M., Peck A.M.,
RA   Derge J.G., Lipman D., Collins F.S., Jang W., Sherry S., Feolo M.,
RA   Misquitta L., Lee E., Rotmistrovsky K., Greenhut S.F., Schaefer C.F.,
RA   Buetow K., Bonner T.I., Haussler D., Kent J., Kiekhaus M., Furey T.,
RA   Brent M., Prange C., Schreiber K., Shapiro N., Bhat N.K., Hopkins R.F.,
RA   Hsie F., Driscoll T., Soares M.B., Casavant T.L., Scheetz T.E.,
RA   Brown-stein M.J., Usdin T.B., Toshiyuki S., Carninci P., Piao Y.,
RA   Dudekula D.B., Ko M.S., Kawakami K., Suzuki Y., Sugano S., Gruber C.E.,
RA   Smith M.R., Simmons B., Moore T., Waterman R., Johnson S.L., Ruan Y.,
RA   Wei C.L., Mathavan S., Gunaratne P.H., Wu J., Garcia A.M., Hulyk S.W.,
RA   Fuh E., Yuan Y., Sneed A., Kowis C., Hodgson A., Muzny D.M., McPherson J.,
RA   Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Madari A., Young A.C., Wetherby K.D., Granite S.J.,
RA   Kwong P.N., Brinkley C.P., Pearson R.L., Bouffard G.G., Blakesly R.W.,
RA   Green E.D., Dickson M.C., Rodriguez A.C., Grimwood J., Schmutz J.,
RA   Myers R.M., Butterfield Y.S., Griffith M., Griffith O.L., Krzywinski M.I.,
RA   Liao N., Morin R., Morrin R., Palmquist D., Petrescu A.S., Skalska U.,
RA   Smailus D.E., Stott J.M., Schnerch A., Schein J.E., Jones S.J., Holt R.A.,
RA   Baross A., Marra M.A., Clifton S., Makowski K.A., Bosak S., Malek J.;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [9] {ECO:0000313|EMBL:BAE39825.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=BALB/C {ECO:0000313|EMBL:BAE39825.1};
RA   Arakawa T., Carninci P., Fukuda S., Hashizume W., Hayashida K., Hori F.,
RA   Iida J., Imamura K., Imotani K., Itoh M., Kanagawa S., Kawai J., Kojima M.,
RA   Konno H., Murata M., Nakamura M., Ninomiya N., Nishiyori H., Nomura K.,
RA   Ohno M., Sakazume N., Sano H., Sasaki D., Shibata K., Shiraki T.,
RA   Tagami M., Tagami Y., Waki K., Watahiki A., Muramatsu M., Hayashizaki Y.;
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN   [10] {ECO:0000313|EMBL:BAB29074.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=BALB/C {ECO:0000313|EMBL:BAE39825.1}, C57BL/6J
RC   {ECO:0000313|EMBL:BAB29074.1}, and NOD {ECO:0000313|EMBL:BAC40467.1};
RC   TISSUE=Head {ECO:0000313|EMBL:BAB29074.1}, and Thymus
RC   {ECO:0000313|EMBL:BAC34363.1};
RG   The FANTOM Consortium;
RG   Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group);
RT   "The Transcriptional Landscape of the Mammalian Genome.";
RL   Science 309:1559-1563(2005).
RN   [11] {ECO:0000313|EMBL:BAB29074.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=BALB/C {ECO:0000313|EMBL:BAE39825.1}, C57BL/6J
RC   {ECO:0000313|EMBL:BAB29074.1}, and NOD {ECO:0000313|EMBL:BAC40467.1};
RC   TISSUE=Head {ECO:0000313|EMBL:BAB29074.1}, and Thymus
RC   {ECO:0000313|EMBL:BAC34363.1};
RX   PubMed=16141073; DOI=10.1126/science.1112009;
RG   RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group) and the FANTOM Consortium;
RT   "Antisense Transcription in the Mammalian Transcriptome.";
RL   Science 309:1564-1566(2005).
CC   -!- SUBCELLULAR LOCATION: Cell projection, lamellipodium membrane
CC       {ECO:0000256|ARBA:ARBA00004588}; Peripheral membrane protein
CC       {ECO:0000256|ARBA:ARBA00004588}; Cytoplasmic side
CC       {ECO:0000256|ARBA:ARBA00004588}. Cell projection, ruffle membrane
CC       {ECO:0000256|ARBA:ARBA00004599}; Peripheral membrane protein
CC       {ECO:0000256|ARBA:ARBA00004599}; Cytoplasmic side
CC       {ECO:0000256|ARBA:ARBA00004599}.
CC   -!- SIMILARITY: Belongs to the actin-binding proteins ADF family.
CC       {ECO:0000256|ARBA:ARBA00006844}.
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DR   EMBL; BC094357; AAH94357.1; -; mRNA.
DR   EMBL; AK013943; BAB29074.1; -; mRNA.
DR   EMBL; AK050651; BAC34363.1; -; mRNA.
DR   EMBL; AK088633; BAC40467.1; -; mRNA.
DR   EMBL; AK088793; BAC40575.1; -; mRNA.
DR   EMBL; AK167795; BAE39825.1; -; mRNA.
DR   RefSeq; NP_031713.1; NM_007687.5.
DR   AlphaFoldDB; Q544Y7; -.
DR   SMR; Q544Y7; -.
DR   SwissPalm; Q544Y7; -.
DR   MaxQB; Q544Y7; -.
DR   Antibodypedia; 16010; 1223 antibodies from 41 providers.
DR   DNASU; 12631; -.
DR   GeneID; 12631; -.
DR   KEGG; mmu:12631; -.
DR   AGR; MGI:101757; -.
DR   CTD; 1072; -.
DR   MGI; MGI:101757; Cfl1.
DR   VEuPathDB; HostDB:ENSMUSG00000056201; -.
DR   OMA; WSMIYAT; -.
DR   OrthoDB; 3380386at2759; -.
DR   BioGRID-ORCS; 12631; 7 hits in 23 CRISPR screens.
DR   ChiTaRS; Cfl1; mouse.
DR   ExpressionAtlas; Q544Y7; baseline and differential.
DR   GO; GO:0015629; C:actin cytoskeleton; IEA:InterPro.
DR   GO; GO:0031258; C:lamellipodium membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR   GO; GO:0032587; C:ruffle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0051015; F:actin filament binding; IEA:UniProt.
DR   GO; GO:0030042; P:actin filament depolymerization; IEA:InterPro.
DR   CDD; cd11286; ADF_cofilin_like; 1.
DR   Gene3D; 3.40.20.10; Severin; 1.
DR   InterPro; IPR002108; ADF-H.
DR   InterPro; IPR029006; ADF-H/Gelsolin-like_dom_sf.
DR   InterPro; IPR017904; ADF/Cofilin.
DR   PANTHER; PTHR11913:SF17; COFILIN-1; 1.
DR   PANTHER; PTHR11913; COFILIN-RELATED; 1.
DR   Pfam; PF00241; Cofilin_ADF; 1.
DR   PRINTS; PR00006; COFILIN.
DR   SMART; SM00102; ADF; 1.
DR   SUPFAM; SSF55753; Actin depolymerizing proteins; 1.
DR   PROSITE; PS51263; ADF_H; 1.
PE   2: Evidence at transcript level;
KW   Actin-binding {ECO:0000256|ARBA:ARBA00023203};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136};
KW   Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW   Phosphoprotein {ECO:0000256|ARBA:ARBA00022553}.
FT   DOMAIN          4..153
FT                   /note="ADF-H"
FT                   /evidence="ECO:0000259|PROSITE:PS51263"
SQ   SEQUENCE   166 AA;  18560 MW;  19834E8CA80747B2 CRC64;
     MASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE EGKEILVGDV
     GQTVDDPYTT FVKMLPDKDC RYALYDATYE TKESKKEDLV FIFWAPENAP LKSKMIYASS
     KDAIKKKLTG IKHELQANCY EEVKDRCTLA EKLGGSAVIS LEGKPL
//