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Protein

Beta-lactamase

Gene

sme-2

Organism
Serratia marcescens
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.SAAS annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

HydrolaseSAAS annotation

Keywords - Biological processi

Antibiotic resistanceSAAS annotation

Protein family/group databases

MEROPSiS11.A01.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamaseSAAS annotation (EC:3.5.2.6SAAS annotation)
Gene namesi
Name:sme-2Imported
Synonyms:blaSME-1Imported, blaSME-4Imported, blaSME1Imported
ORF Names:SME12620_22Imported
OrganismiSerratia marcescensImported
Taxonomic identifieri615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence analysisAdd
BLAST
Chaini28 – 294267Beta-lactamaseSequence analysisPRO_5007704272Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi72 ↔ 242Combined sources

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DY6X-ray2.13A/B28-294[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.SAAS annotation

Keywords - Domaini

SignalSequence analysis

Phylogenomic databases

KOiK19316.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54488-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNKVNFKTA SFLFSVCLAL SAFNAHANKS DAAAKQIKKL EEDFDGRIGV
60 70 80 90 100
FAIDTGSGNT FGYRSDERFP LCSSFKGFLA AAVLERVQQK KLDINQKVKY
110 120 130 140 150
ESRDLEYHSP ITTKYKGSGM TLGDMASAAL QYSDNGATNI IMERFLGGPE
160 170 180 190 200
GMTKFMRSIG DNEFRLDRWE LELNTAIPGD KRDTSTPKAV ANSLNKLALG
210 220 230 240 250
NVLNAKVKAI YQNWLKGNTT GDARIRASVP ADWVVGDKTG SCGAYGTAND
260 270 280 290
YAVIWPKNRA PLIVSIYTTR KSKDDKHSDK TIAEASRIAI QAID
Length:294
Mass (Da):32,247
Last modified:November 1, 1996 - v1
Checksum:iC326C0DC65293094
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60295 Genomic DNA. Translation: AAB03414.1.
JF974075 Genomic DNA. Translation: AEJ79644.1.
KF615855 Genomic DNA. Translation: AGZ03855.1.
KF481967 Genomic DNA. Translation: AHA49908.1.

Genome annotation databases

KEGGiag:AHA49908.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60295 Genomic DNA. Translation: AAB03414.1.
JF974075 Genomic DNA. Translation: AEJ79644.1.
KF615855 Genomic DNA. Translation: AGZ03855.1.
KF481967 Genomic DNA. Translation: AHA49908.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DY6X-ray2.13A/B28-294[»]
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS11.A01.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AHA49908.

Phylogenomic databases

KOiK19316.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequence analysis of the gene for a carbapenem-hydrolyzing class A beta-lactamase, Sme-1, from Serratia marcescens S6."
    Naas T., Vandel L., Sougakoff W., Livermore D.M., Nordmann P.
    Antimicrob. Agents Chemother. 38:1262-1270(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: S6Imported.
  2. "Carbapenem-hydrolyzing beta-lactamase, Sme-2."
    Rasmussen B.A., Keeney D., Cohen C.
    Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: S6Imported.
  3. "Structure of the imipenem-hydrolyzing class A beta-lactamase SME-1 from Serratia marcescens."
    Sougakoff W., L'Hermite G., Pernot L., Naas T., Guillet V., Nordmann P., Jarlier V., Delettre J.
    Acta Crystallogr. D 58:267-274(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.13 ANGSTROMS) OF 28-294, DISULFIDE BONDS.
  4. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: SMDET-2Imported.
  5. Qi P., Wei Y., Zheng Y.
    Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: SMDET-2Imported.
  6. "Characterization of carbapenem resistant isolates from 2012."
    Farrell S.E., Castanheira M., Jones R.N.
    Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 41727RImported.
  7. "Serratia marcescens harbouring SME-type class A carbapenemases in Canada and the presence of blaSME on a novel genomic island, SmarGI1-1."
    Mataseje L.F., Boyd D.A., Delport J., Hoang L., Imperial M., Lefebvre B., Kuhn M., Van Caeseele P., Willey B.M., Mulvey M.R.
    J. Antimicrob. Chemother. 69:1825-1829(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: N12-0620Imported.

Entry informationi

Entry nameiQ54488_SERMA
AccessioniPrimary (citable) accession number: Q54488
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.