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Protein

Mercuric reductase

Gene

merA

Organism
Shewanella putrefaciens (Pseudomonas putrefaciens)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Resistance to Hg2+ in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg0.

Miscellaneous

The active site is a redox-active disulfide bond.

Catalytic activityi

Hg + NADP+ + H+ = Hg2+ + NADPH.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi554MercuryPROSITE-ProRule annotation1
Metal bindingi555MercuryPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi124 – 133FADBy similarity10

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processMercuric resistance
LigandFAD, Flavoprotein, Mercury, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Mercuric reductase (EC:1.16.1.1)
Alternative name(s):
Hg(II) reductase
Gene namesi
Name:merA
Encoded oniPlasmid IncJ pMERPH0 Publication
OrganismiShewanella putrefaciens (Pseudomonas putrefaciens)
Taxonomic identifieri24 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000679991 – 557Mercuric reductaseAdd BLAST557

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi133 ↔ 138Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ54465

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ54465
SMRiQ54465
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 66HMAPROSITE-ProRule annotationAdd BLAST66

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Family and domain databases

CDDicd00371 HMA, 1 hit
Gene3Di3.30.390.30, 1 hit
3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR016156 FAD/NAD-linked_Rdtase_dimer_sf
IPR017969 Heavy-metal-associated_CS
IPR006121 HMA_dom
IPR036163 HMA_dom_sf
IPR021179 Mercury_reductase_MerA
IPR001100 Pyr_nuc-diS_OxRdtase
IPR004099 Pyr_nucl-diS_OxRdtase_dimer
IPR012999 Pyr_OxRdtase_I_AS
PfamiView protein in Pfam
PF00403 HMA, 1 hit
PF07992 Pyr_redox_2, 1 hit
PF02852 Pyr_redox_dim, 1 hit
PIRSFiPIRSF000350 Mercury_reductase_MerA, 1 hit
SUPFAMiSSF51905 SSF51905, 3 hits
SSF55008 SSF55008, 1 hit
SSF55424 SSF55424, 1 hit
TIGRFAMsiTIGR02053 MerA, 1 hit
PROSITEiView protein in PROSITE
PS01047 HMA_1, 1 hit
PS50846 HMA_2, 1 hit
PS00076 PYRIDINE_REDOX_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q54465-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILLSIEGMT CPSCVAHVKE ALDAIEGVNK VEISYENARA TITTNGGVSV
60 70 80 90 100
TVLIGAIEAL GYIAKESTGT AKEITSPNDC CDNENASNTE SNQTQHVAII
110 120 130 140 150
GTGSGAFACA IKAAEGGAKV TLIEGADVIG GCCVNVGCVP SKILIRAAQL
160 170 180 190 200
AQQQRNNPFT GLENHAPQLS RALLTQQQTA RVEELRAAKY QNILETNPAL
210 220 230 240 250
SLLKGWAQFK NANTLIVRKN DGTEQAVHAD KILIATGSTP TIPPIDGLTE
260 270 280 290 300
TPYWTSTEAL FAQELPQHLV VIGSSVVALE IAQAYRRLGS EVTILARHTL
310 320 330 340 350
LYREDPLLGE KLTGCFEKEG IRVLNSTQAT KVTHDGSQFT LETNAGDLRC
360 370 380 390 400
DRLLVSTGRH ANTCQLNLGA VGVTTNKKGE IVVNERMETN VPGIYAAGDC
410 420 430 440 450
CNMPQFVYVA AAAGSRSGIN MTGGYAKLDL STMPAVIFTD PQVATVGLTE
460 470 480 490 500
EQANAQDIET DSRVLEMENV PRALANFETD GFIKLVTEKA TGRLIGAQIL
510 520 530 540 550
AHEGGELIQS AALAIRNRMT VTELADQLFP YLTMVEGLKL CAQTFNKDVK

ELSCCAG
Length:557
Mass (Da):59,336
Last modified:November 1, 1996 - v1
Checksum:iEE3D6E1C2ACD473C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49196 Genomic DNA Translation: CAA89057.1

Similar proteinsi

Entry informationi

Entry nameiMERA_SHEPU
AccessioniPrimary (citable) accession number: Q54465
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

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