Q54410 (TAP_STRLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tripeptidyl aminopeptidase
Short name=Tap
EC=3.4.14.-

Gene names

Name:

tap

Organism

Streptomyces lividans

Taxonomic identifier

1916 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	537 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cleaves tripeptides from the N-termini of proteins. Does not cleave mono- or dipeptides, or N-terminally blocked peptides. Ref.1
Subcellular location	Secreted Ref.1.
Sequence similarities	Belongs to the peptidase S33 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	proteolysis Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	extracellular region Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	tripeptide aminopeptidase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 36	36	Potential
	Propeptide	37 – 39	3	Ref.1		PRO_0000401063
	Chain	40 – 537	498	Tripeptidyl aminopeptidase Ref.1		PRO_5000142441
Regions
	Compositional bias	10 – 74	65	Ala-rich
Sites
	Active site	245	1	Nucleophile By similarity
	Active site	470	1	By similarity
	Active site	499	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54410 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 850703374BC4DEC9	FASTA	537	58,273
	10 20 30 40 50 60 MRKSSIRRRA TAFGTAGALV TATLIAGAVS APAASAAPAD GHGHGRSWDR EARGAAIAAA 70 80 90 100 110 120 RAARAGIDWE DCAADWNLPK PIQCGYVTVP MDYAKPYGKQ IRLAVDRIGN TGTRSERQGA 130 140 150 160 170 180 LIYNPGGPGG SGLRFPARVT NKSAVWANTA KAYDFVGFDP RGVGHSAPIS CVDPQEFVKA 190 200 210 220 230 240 PKADPVPGSE ADKRAQRKLA REYAEGCFER SGEMLPHMTT PNTARDLDVI RAALGEKKLN 250 260 270 280 290 300 YLGVSYGTYL GAVYGTLFPD HVRRMVVDSV VNPSRDKIWY QANLDQDVAF EGRWKDWQDW 310 320 330 340 350 360 VAANDAAYHL GDTRAEVQDQ WLKLRAAAAK KPLGGVVGPA ELISFFQSAP YYDSAWAPTA 370 380 390 400 410 420 EIFSKYVAGD TQALVDAAAP DLSDTAGNAS AENGNAVYTA VECTDAKWPA NWRTWDRDNT 430 440 450 460 470 480 RLHRDHPFMT WANAWMNLPC ATWPVKQQTP LNVKTGKGLP PVLIVQSERD AATPYEGAVE 490 500 510 520 530 LHQRFRGSRL ITERDAGSHG VTGLVNPCIN DRVDTYLLTG RTDARDVTCA PHATPRP

References

[1]

"Cloning and characterization of a gene encoding a secreted tripeptidyl aminopeptidase from Streptomyces lividans 66." Butler M.J., Binnie C., DiZonno M.A., Krygsman P., Soltes G.A., Soostmeyer G., Walczyk E., Malek L.T. Appl. Environ. Microbiol. 61:3145-3150(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 40-50, FUNCTION, SUBCELLULAR LOCATION. Strain: 66 / 1326.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L27466 Genomic DNA. Translation: AAA92338.1.
3D structure databases
ProteinModelPortal	Q54410.
ModBase	Search...
Protein family/group databases
MEROPS	S33.002.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013595. Pept_S33_TAP-like_C. [Graphical view]
Pfam	PF08386. Abhydrolase_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TAP_STRLI

Accession

Primary (citable) accession number: Q54410

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2010
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program