Q54331 (Q54331_STRLI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Cellulase B EMBL AAB71950.1

Gene names

Name:

celB EMBL AAB71950.1

Organism

Streptomyces lividans EMBL AAB71950.1

Taxonomic identifier

1916 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

Ontologies

Keywords
Domain	Signal EMBL AAB71950.1
Technical term	3D-structure PDB 2NLR PDB 1NLR
Gene Ontology (GO)
Biological process	polysaccharide catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cellulase activity Inferred from electronic annotation. Source: InterPro polysaccharide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 40	40	Potential EMBL AAB71950.1
	Chain	41 – 381	341	cellulase B EMBL AAB71950.1		PRO_5000144050

Sequences

	Sequence		Length	Mass (Da)
	Q54331 [UniParc]. Last modified January 1, 1998. Version 2. Checksum: A7E99BF590FA24EC	FASTA	381	39,239
	10 20 30 40 50 60 MRTLRPQARA PRGLLAALGA VLAAFALVSS LVTAAAPAQA DTTICEPFGT TTIQGRYVVQ 70 80 90 100 110 120 NNRWGSTAPQ CVTATDTGFR VTQADGSAPT NGAPKSYPSV FNGCHYTNCS PGTDLPVRLD 130 140 150 160 170 180 TVSAAPSSIS YGFVDGAVYN ASYDIWLDPT ARTDGVNQTE IMIWFNRVGP IQPIGSPVGT 190 200 210 220 230 240 ASVGGRTWEV WSGGNGSNDV LSFVAPSAIS GWSFDVMDFV RATVARGLAE NDWYLTSVQA 250 260 270 280 290 300 GFEPWQNGAG LAVNSFSSTV ETGTPGGTDP GDPGGPSACA VSYGTNVWQD GFTADVTVTN 310 320 330 340 350 360 TGTAPVDGWQ LAFTLPSGQR ITNAWNASLT PSSGSVTATG ASHNARIAPG GSLSFGFQGT 370 380 YGGAFAEPTG FRLNGTACTT V

References

[1]	"Purification and characterization of the CelB endoglucanase from Streptomyces lividans 66 and DNA sequence of the encoding gene." Wittmann S., Shareck F., Kluepfel D., Morosoli R. Appl. Environ. Microbiol. 60:1701-1703(1994) [PubMed: 8017952] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: 66 EMBL AAB71950.1.
[2]	Shareck F. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: 66 EMBL AAB71950.1.
[3]	"The crystal structure of a 2-fluorocellotriosyl complex of the Streptomyces lividans endoglucanase CelB2 at 1.2 A resolution." Sulzenbacher G., Mackenzie L.F., Wilson K.S., Withers S.G., Dupont C., Davies G.J. Biochemistry 38:4826-4833(1999) [PubMed: 10200171] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.20 ANGSTROMS) OF 41-274.
[4]	"The Streptomyces lividans family 12 endoglucanase: construction of the catalytic cre, expression, and X-ray structure at 1.75 A resolution." Sulzenbacher G., Shareck F., Morosoli R., Dupont C., Davies G.J. Biochemistry 36:16032-16039(1997) [PubMed: 9440876] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 41-274.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U04629 Genomic DNA. Translation: AAB71950.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1NLR	X-ray	1.75	A	41-274	[»]
2NLR	X-ray	1.20	A	41-274	[»]

ProteinModelPortal

Q54331.

SMR

Q54331. Positions 41-262, 274-379.

ModBase

Search...

Protein family/group databases

CAZy

CBM2. Carbohydrate-Binding Module Family 2.
GH12. Glycoside Hydrolase Family 12.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR008965. Carb-bd_dom.
IPR012291. CBD_carb-bd_dom.
IPR001919. Cellulose-bd_dom_fam2_bac.
IPR008985. ConA-like_lec_gl.
IPR013319. Glyco_hydro_11/12_cat.
IPR002594. Glyco_hydro_12.
[Graphical view]

Gene3D

G3DSA:2.60.40.290. CBD_carb_bd. 1 hit.
G3DSA:2.60.120.180. Glyco_hydro_11/12_cat. 1 hit.

Pfam

PF00553. CBM_2. 1 hit.
PF01670. Glyco_hydro_12. 1 hit.
[Graphical view]

SMART

SM00637. CBD_II. 1 hit.
[Graphical view]

SUPFAM

SSF49384. Cellul_bind. 1 hit.
SSF49899. ConA_like_lec_gl. 1 hit.

PROSITE

PS51173. CBM2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q54331_STRLI

Accession

Primary (citable) accession number: Q54331

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)