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Protein

L-lysine cyclodeaminase

Gene

rapL

Organism
Streptomyces rapamycinicus (strain ATCC 29253 / DSM 41530 / NRRL 5491 / AYB-994) (Streptomyces hygroscopicus (strain ATCC 29253))
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts L-lysine to L-pipecolate, which is incorporated into multiple secondary metabolite products, including rapamycin, tobulysin, virginiamycin and pristinamycin.1 Publication

Catalytic activityi

L-lysine = L-pipecolate + NH3.1 Publication

Cofactori

NAD+1 Publication

Enzyme regulationi

Inhibited by nipecotic acid and thiazolidine-2-carboxylic acid.1 Publication

Kineticsi

kcat is 0.61 min(-1) with L-lysine as substrate.
  1. KM=46 µM for L-lysine1 Publication

    Pathwayi: Antibiotic biosynthesis

    This protein is involved in Antibiotic biosynthesis.1 Publication
    View all proteins of this organism that are known to be involved in Antibiotic biosynthesis.

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionLyase
    Biological processAntibiotic biosynthesis
    LigandNAD

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17627

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-lysine cyclodeaminase (EC:4.3.1.28)
    Alternative name(s):
    Rapamycin biosynthesis protein L
    Gene namesi
    Name:rapL
    ORF Names:M271_40660Imported
    OrganismiStreptomyces rapamycinicus (strain ATCC 29253 / DSM 41530 / NRRL 5491 / AYB-994) (Streptomyces hygroscopicus (strain ATCC 29253))
    Taxonomic identifieri1343740 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces
    Proteomesi
    • UP000030183 Componenti: Chromosome

    Pathology & Biotechi

    Disruption phenotypei

    Cells do not produce significant amounts of rapamycin. Cells supplemented by L-pipecolate restore levels of rapamycin.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004239871 – 343L-lysine cyclodeaminaseAdd BLAST343

    Structurei

    3D structure databases

    ProteinModelPortaliQ54304
    SMRiQ54304
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    KOiK20500

    Family and domain databases

    Gene3Di3.30.1780.10, 2 hits
    InterProiView protein in InterPro
    IPR036291 NAD(P)-bd_dom_sf
    IPR003462 ODC_Mu_crystall
    IPR023401 ODC_N
    PANTHERiPTHR13812 PTHR13812, 1 hit
    PfamiView protein in Pfam
    PF02423 OCD_Mu_crystall, 1 hit
    PIRSFiPIRSF001439 CryM, 1 hit
    SUPFAMiSSF51735 SSF51735, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q54304-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MQTKVLCQRD IKRILSVVGR DVMMDRLISE VHAGFARLGR GETDEPPPRT
    60 70 80 90 100
    GFARGGDVPG VIEFMPHRAS GIGVTMKTVS YSPQNFERFN LPTIVGTVSR
    110 120 130 140 150
    LDDDSGSMVA LADAATITAM RTGAVAAVAT RLLARPGSTT LALIGAGAQA
    160 170 180 190 200
    VTQAHALSRV LPLERILISD IKAEHAESFA GRVAFLELPV EVTDAATAMA
    210 220 230 240 250
    TADVLCTVTS VPVGGGPVVP AEPRQAHLHV NGIGADEQGK TELPKALLDD
    260 270 280 290 300
    AFICVDHPGQ ARAEGEFQQL PDRELGPSLA DLCAAPEIAA PHPERLSVFD
    310 320 330 340
    STGSAFADHI ALDVLLGFAD ELGLGHKMSI ESTPEDVLDP YSL
    Length:343
    Mass (Da):36,091
    Last modified:May 23, 2018 - v2
    Checksum:i84617294E585BB51
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti50T → P in CAA60467 (PubMed:8635756).1
    Sequence conflicti84Q → E in CAA60467 (PubMed:8635756).1
    Sequence conflicti102D → G in CAA60467 (PubMed:8635756).1
    Sequence conflicti127 – 129AVA → SVT in CAA60467 (PubMed:8635756).3

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X86780 Genomic DNA Translation: CAA60467.1
    CP006567 Genomic DNA Translation: AGP59511.1
    PIRiT30233

    Genome annotation databases

    EnsemblBacteriaiAGP59511; AGP59511; M271_40660
    KEGGiag:CAA60467
    PATRICifig|1343740.8.peg.6275

    Similar proteinsi

    Entry informationi

    Entry nameiRAPL_STRRN
    AccessioniPrimary (citable) accession number: Q54304
    Secondary accession number(s): A0A0A0NQJ4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
    Last sequence update: May 23, 2018
    Last modified: May 23, 2018
    This is version 63 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

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