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Q54277 (DYR_STAHA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrofolate reductase

EC=1.5.1.3
Gene names
Name:dfrD
Encoded onPlasmid pABU17
OrganismStaphylococcus haemolyticus
Taxonomic identifier1283 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length166 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Miscellaneous

Confers trimethoprim resistance.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Mass spectrometry

Molecular mass is 19821.2±2 Da from positions 1 - 166. Determined by ESI. Ref.1

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 166166Dihydrofolate reductase
PRO_0000186416

Regions

Domain6 – 164159DHFR
Nucleotide binding11 – 122NADP By similarity
Nucleotide binding19 – 246NADP By similarity
Nucleotide binding48 – 514NADP By similarity
Nucleotide binding67 – 704NADP By similarity
Nucleotide binding100 – 1056NADP By similarity
Region10 – 123Substrate binding By similarity

Sites

Binding site321Substrate By similarity
Binding site621Substrate By similarity
Binding site1191Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54277 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 9AAD03F3ECCA9990

FASTA16619,825
        10         20         30         40         50         60 
MWSFLKISLI VAMDKKRVIG KDNDIPWRIS SDWEYVKNTT KGHAIILGRK NLQSIGRALP 

        70         80         90        100        110        120 
DRRNIILTRD KNFNFKDCEI AHSIEAAFKL CENEEEVFIF GGEQIYVMFL PYVEKMYVTK 

       130        140        150        160 
IHHEFEGDTF FPVVNFDDWK EVSVEKGIKD EKNPYDYYFH IYERIR 

« Hide

References

[1]"Cloning and characterization of a novel, plasmid-encoded trimethoprim-resistant dihydrofolate reductase from Staphylococcus haemolyticus MUR313."
Dale G.E., Langen H., Page M.G., Then R.L., Stueber D.
Antimicrob. Agents Chemother. 39:1920-1924(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MASS SPECTROMETRY.
Strain: MUR313.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z50141 Genomic DNA. Translation: CAA90486.1.

3D structure databases

ProteinModelPortalQ54277.
SMRQ54277. Positions 7-164.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00077; UER00158.

Family and domain databases

Gene3D3.40.430.10. 1 hit.
InterProIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFPIRSF000194. DHFR. 1 hit.
PRINTSPR00070. DHFR.
SUPFAMSSF53597. SSF53597. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDYR_STAHA
AccessionPrimary (citable) accession number: Q54277
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 16, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways