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Protein

Dihydrofolate reductase

Gene

dfrD

Organism
Staphylococcus haemolyticus
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Miscellaneous

Confers trimethoprim resistance.

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (AL503_010535), Dihydrofolate reductase (dfrD), Dihydrofolate reductase (dfrD), Dihydrofolate reductase (B8W97_10220)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei32SubstrateBy similarity1
Binding sitei62SubstrateBy similarity1
Binding sitei119SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 12NADPBy similarity2
Nucleotide bindingi19 – 24NADPBy similarity6
Nucleotide bindingi48 – 51NADPBy similarity4
Nucleotide bindingi67 – 70NADPBy similarity4
Nucleotide bindingi100 – 105NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAntibiotic resistance, Methotrexate resistance, One-carbon metabolism, Trimethoprim resistance
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:dfrD
Encoded oniPlasmid pABU170 Publication
OrganismiStaphylococcus haemolyticus
Taxonomic identifieri1283 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001864161 – 166Dihydrofolate reductaseAdd BLAST166

Structurei

3D structure databases

ProteinModelPortaliQ54277
SMRiQ54277
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 164DHFRPROSITE-ProRule annotationAdd BLAST159

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni10 – 12Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

KOiK18591

Family and domain databases

CDDicd00209 DHFR, 1 hit
Gene3Di3.40.430.10, 1 hit
InterProiView protein in InterPro
IPR012259 DHFR
IPR024072 DHFR-like_dom_sf
IPR017925 DHFR_CS
IPR001796 DHFR_dom
PANTHERiPTHR22778:SF16 PTHR22778:SF16, 1 hit
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
PIRSFiPIRSF000194 DHFR, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q54277-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWSFLKISLI VAMDKKRVIG KDNDIPWRIS SDWEYVKNTT KGHAIILGRK
60 70 80 90 100
NLQSIGRALP DRRNIILTRD KNFNFKDCEI AHSIEAAFKL CENEEEVFIF
110 120 130 140 150
GGEQIYVMFL PYVEKMYVTK IHHEFEGDTF FPVVNFDDWK EVSVEKGIKD
160
EKNPYDYYFH IYERIR
Length:166
Mass (Da):19,825
Last modified:November 1, 1996 - v1
Checksum:i9AAD03F3ECCA9990
GO

Mass spectrometryi

Molecular mass is 19821.2±2 Da from positions 1 - 166. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50141 Genomic DNA Translation: CAA90486.1

Genome annotation databases

KEGGiag:CAA90486

Similar proteinsi

Entry informationi

Entry nameiDYR_STAHA
AccessioniPrimary (citable) accession number: Q54277
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

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