Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q54276

- Q54276_SERMA

UniProt

Q54276 - Q54276_SERMA

Protein
Submitted name:

Chitinase

Gene

chiB

Organism
Serratia marcescens
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei12 – 121N-acetyl-D-glucosamineImported
    Binding sitei56 – 561Sulfate 5
    Binding sitei89 – 891Sulfate 6
    Binding sitei190 – 1901Sulfate 8; via carbonyl oxygen
    Binding sitei191 – 1911N-acetyl-D-glucosamine
    Binding sitei194 – 1941Sulfate 8
    Binding sitei220 – 2201N-acetyl-D-glucosamine
    Binding sitei264 – 2641Sulfate 9; via amide nitrogen
    Binding sitei282 – 2821Sulfate 10
    Binding sitei294 – 2941N-acetyl-D-glucosamine
    Binding sitei316 – 3161N-acetyl-D-glucosamine
    Binding sitei343 – 3431Sulfate 16
    Binding sitei403 – 4031N-acetyl-D-glucosamineImported
    Binding sitei407 – 4071N-acetyl-D-glucosamineImported
    Binding sitei410 – 4101Sulfate 16
    Binding sitei439 – 4391Sulfate 9; via carbonyl oxygen

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. chitinase activity Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. chitin catabolic process Source: InterPro

    Keywords - Molecular functioni

    GlycosidaseUniRule annotation, Hydrolase

    Protein family/group databases

    CAZyiCBM5. Carbohydrate-Binding Module Family 5.
    GH18. Glycoside Hydrolase Family 18.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    ChitinaseImported
    Gene namesi
    Name:chiBImported
    OrganismiSerratia marcescensImported
    Taxonomic identifieri615 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 499499chitinaseImportedPRO_5000147550Add
    BLAST

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1E15X-ray1.90A/B1-499[»]
    1E6PX-ray1.70A/B1-499[»]
    1E6RX-ray2.50A/B1-499[»]
    1E6ZX-ray1.99A/B2-499[»]
    1GPFX-ray1.85A/B1-499[»]
    1UR8X-ray1.90A/B1-499[»]
    1UR9X-ray1.80A/B1-498[»]
    1W1PX-ray2.10A/B1-499[»]
    1W1TX-ray1.90A/B1-499[»]
    1W1VX-ray1.85A/B1-499[»]
    1W1YX-ray1.85A/B1-499[»]
    ProteinModelPortaliQ54276.
    SMRiQ54276. Positions 2-499.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ54276.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni97 – 982N-acetyl-D-glucosamine bindingImported
    Regioni142 – 1454N-acetyl-D-glucosamine binding
    Regioni214 – 2152N-acetyl-D-glucosamine binding

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 18 family.UniRule annotation

    Family and domain databases

    Gene3Di2.10.10.20. 1 hit.
    3.10.50.10. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR003610. CBM_fam5/12.
    IPR011583. Chitinase_II.
    IPR029070. Chitinase_insertion.
    IPR001223. Glyco_hydro18cat.
    IPR001579. Glyco_hydro_18_chit_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02839. CBM_5_12. 1 hit.
    PF00704. Glyco_hydro_18. 1 hit.
    [Graphical view]
    SMARTiSM00495. ChtBD3. 1 hit.
    SM00636. Glyco_18. 1 hit.
    [Graphical view]
    SUPFAMiSSF51055. SSF51055. 1 hit.
    SSF51445. SSF51445. 2 hits.
    SSF54556. SSF54556. 1 hit.
    PROSITEiPS01095. CHITINASE_18. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q54276-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTRKAVIGY YFIPTNQINN YTETDTSVVP FPVSNITPAK AKQLTHINFS    50
    FLDINSNLEC AWDPATNDAK ARDVVNRLTA LKAHNPSLRI MFSIGGWYYS 100
    NDLGVSHANY VNAVKTPASR AKFAQSCVRI MKDYGFDGVD IDWEYPQAAE 150
    VDGFIAALQE IRTLLNQQTI TDGRQALPYQ LTIAGAGGAF FLSRYYSKLA 200
    QIVAPLDYIN LMTYDLAGPW EKVTNHQAAL FGDAAGPTFY NALREANLGW 250
    SWEELTRAFP SPFSLTVDAA VQQHLMMEGV PSAKIVMGVP FYGRAFKGVS 300
    GGNGGQYSSH STPGEDPYPS TDYWLVGCEE CVRDKDPRIA SYRQLEQMLQ 350
    GNYGYQRLWN DKTKTPYLYH AQNGLFVTYD DAESFKYKAK YIKQQQLGGV 400
    MFWHLGQDNR NGDLLAALDR YFNAADYDDS QLDMGTGLRY TGVGPGNLPI 450
    MTAPAYVPGT TYAQGALVSY QGYVWQTKWG YITSAPGSDS AWLKVGRVA 499
    Length:499
    Mass (Da):55,469
    Last modified:November 1, 1996 - v1
    Checksum:i58C933A9064D526B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z36295 Genomic DNA. Translation: CAA85292.1.
    PIRiS52422.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z36295 Genomic DNA. Translation: CAA85292.1 .
    PIRi S52422.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1E15 X-ray 1.90 A/B 1-499 [» ]
    1E6P X-ray 1.70 A/B 1-499 [» ]
    1E6R X-ray 2.50 A/B 1-499 [» ]
    1E6Z X-ray 1.99 A/B 2-499 [» ]
    1GPF X-ray 1.85 A/B 1-499 [» ]
    1UR8 X-ray 1.90 A/B 1-499 [» ]
    1UR9 X-ray 1.80 A/B 1-498 [» ]
    1W1P X-ray 2.10 A/B 1-499 [» ]
    1W1T X-ray 1.90 A/B 1-499 [» ]
    1W1V X-ray 1.85 A/B 1-499 [» ]
    1W1Y X-ray 1.85 A/B 1-499 [» ]
    ProteinModelPortali Q54276.
    SMRi Q54276. Positions 2-499.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM5. Carbohydrate-Binding Module Family 5.
    GH18. Glycoside Hydrolase Family 18.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q54276.

    Family and domain databases

    Gene3Di 2.10.10.20. 1 hit.
    3.10.50.10. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR003610. CBM_fam5/12.
    IPR011583. Chitinase_II.
    IPR029070. Chitinase_insertion.
    IPR001223. Glyco_hydro18cat.
    IPR001579. Glyco_hydro_18_chit_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02839. CBM_5_12. 1 hit.
    PF00704. Glyco_hydro_18. 1 hit.
    [Graphical view ]
    SMARTi SM00495. ChtBD3. 1 hit.
    SM00636. Glyco_18. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51055. SSF51055. 1 hit.
    SSF51445. SSF51445. 2 hits.
    SSF54556. SSF54556. 1 hit.
    PROSITEi PS01095. CHITINASE_18. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Brurberg M.
      Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "Chitinase B from Serratia marcescens BJL200 is exported to the periplasm without processing."
      Brurberg M.B., Eijsink V.G., Haandrikman A.J., Venema G., Nes I.F.
      Microbiology 141:123-131(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    3. "Structure of a two-domain chitotriosidase from Serratia marcescens at 1.9-A resolution."
      van Aalten D.M., Synstad B., Brurberg M.B., Hough E., Riise B.W., Eijsink V.G., Wierenga R.K.
      Proc. Natl. Acad. Sci. U.S.A. 97:5842-5847(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS).
    4. "Structural insights into the catalytic mechanism of a family 18 exo-chitinase."
      van Aalten D.M., Komander D., Synstad B., Gaseidnes S., Peter M.G., Eijsink V.G.
      Proc. Natl. Acad. Sci. U.S.A. 98:8979-8984(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH N-ACETYL-D-GLUCOSAMINE.
    5. "Psammaplin A, a chitinase inhibitor isolated from the Fijian marine sponge Aplysinella rhax."
      Tabudravu J.N., Eijsink V.G., Gooday G.W., Jaspars M., Komander D., Legg M., Synstad B., van Aalten D.M.
      Bioorg. Med. Chem. 10:1123-1128(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS).
    6. "Interactions of a family 18 chitinase with the designed inhibitor HM508 and its degradation product, chitobiono-delta-lactone."
      Vaaje-Kolstad G., Vasella A., Peter M.G., Netter C., Houston D.R., Westereng B., Synstad B., Eijsink V.G., van Aalten D.M.
      J. Biol. Chem. 279:3612-3619(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 1-498 IN COMPLEX WITH N-ACETYL-D-GLUCOSAMINE.
    7. "Structure-based exploration of cyclic dipeptide chitinase inhibitors."
      Houston D.R., Synstad B., Eijsink V.G., Stark M.J., Eggleston I.M., van Aalten D.M.
      J. Med. Chem. 47:5713-5720(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS).

    Entry informationi

    Entry nameiQ54276_SERMA
    AccessioniPrimary (citable) accession number: Q54276
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 1, 1996
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3