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Q54276 (Q54276_SERMA) Unreviewed, UniProtKB/TrEMBL

Last modified December 11, 2013. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein names
Gene names
Name:chiB EMBL CAA85292.1
OrganismSerratia marcescens EMBL CAA85292.1
Taxonomic identifier615 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the glycosyl hydrolase 18 family. RuleBase RU004453

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 499499chitinase EMBL CAA85292.1
PRO_5000147550

Regions

Region16 – 172Sulfate 2 binding PDB 1UR8 PDB 1UR9
Region97 – 982N-acetyl-D-glucosamine binding PDB 1UR8 PDB 1E6Z PDB 1UR9
Region142 – 1454N-acetyl-D-glucosamine binding
Region214 – 2152N-acetyl-D-glucosamine binding

Sites

Binding site31Sulfate 1 PDB 1UR9
Binding site121N-acetyl-D-glucosamine PDB 1UR8 PDB 1UR9
Binding site171Sulfate 3 PDB 1UR8
Binding site561Sulfate 5
Binding site891Sulfate 6
Binding site1901Sulfate 8; via carbonyl oxygen
Binding site1911N-acetyl-D-glucosamine PDB 1E6Z
Binding site1941Sulfate 8
Binding site2201N-acetyl-D-glucosamine PDB 1E6Z
Binding site2471Sulfate 6 PDB 1UR9
Binding site2641Sulfate 9; via amide nitrogen
Binding site2821Sulfate 10
Binding site2821Sulfate 7 PDB 1UR8 PDB 1W1Y PDB 1W1P PDB 1W1T PDB 1GPF PDB 1UR9
Binding site2941N-acetyl-D-glucosamine
Binding site2941Sulfate 10 PDB 1UR9
Binding site2941Sulfate 8 PDB 1UR8
Binding site2941Sulfate 9 PDB 1UR9
Binding site3161N-acetyl-D-glucosamine PDB 1E6Z
Binding site3271Sulfate 11; via amide nitrogen PDB 1E6R
Binding site3331Sulfate 2 PDB 1UR8 PDB 1UR9
Binding site3331Sulfate 3 PDB 1UR8
Binding site3431Sulfate 12 PDB 1W1V PDB 1E6R
Binding site3431Sulfate 16
Binding site3431Sulfate 4 PDB 1W1T PDB 1E6P
Binding site3471Sulfate 12 PDB 1W1V PDB 1E6R
Binding site3471Sulfate 5 PDB 1UR9
Binding site4031N-acetyl-D-glucosamine PDB 1UR8 PDB 1UR9
Binding site4071N-acetyl-D-glucosamine PDB 1UR8 PDB 1UR9
Binding site4101Sulfate 12 PDB 1W1V PDB 1E6R
Binding site4101Sulfate 16
Binding site4101Sulfate 4 PDB 1W1T PDB 1E6P
Binding site4101Sulfate 5 PDB 1UR9
Binding site4201Sulfate 13 PDB 1UR8 PDB 1E6Z PDB 1W1V PDB 1W1Y PDB 1W1P PDB 1E6R PDB 1W1T PDB 1E6P PDB 1GPF PDB 1UR9
Binding site4201Sulfate 14 PDB 1E6P
Binding site4391Sulfate 9; via carbonyl oxygen
Binding site4551Sulfate 15; via amide nitrogen PDB 1E6R PDB 1W1T

Sequences

Sequence LengthMass (Da)Tools
Q54276 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 58C933A9064D526B

FASTA49955,469
        10         20         30         40         50         60 
MSTRKAVIGY YFIPTNQINN YTETDTSVVP FPVSNITPAK AKQLTHINFS FLDINSNLEC 

        70         80         90        100        110        120 
AWDPATNDAK ARDVVNRLTA LKAHNPSLRI MFSIGGWYYS NDLGVSHANY VNAVKTPASR 

       130        140        150        160        170        180 
AKFAQSCVRI MKDYGFDGVD IDWEYPQAAE VDGFIAALQE IRTLLNQQTI TDGRQALPYQ 

       190        200        210        220        230        240 
LTIAGAGGAF FLSRYYSKLA QIVAPLDYIN LMTYDLAGPW EKVTNHQAAL FGDAAGPTFY 

       250        260        270        280        290        300 
NALREANLGW SWEELTRAFP SPFSLTVDAA VQQHLMMEGV PSAKIVMGVP FYGRAFKGVS 

       310        320        330        340        350        360 
GGNGGQYSSH STPGEDPYPS TDYWLVGCEE CVRDKDPRIA SYRQLEQMLQ GNYGYQRLWN 

       370        380        390        400        410        420 
DKTKTPYLYH AQNGLFVTYD DAESFKYKAK YIKQQQLGGV MFWHLGQDNR NGDLLAALDR 

       430        440        450        460        470        480 
YFNAADYDDS QLDMGTGLRY TGVGPGNLPI MTAPAYVPGT TYAQGALVSY QGYVWQTKWG 

       490 
YITSAPGSDS AWLKVGRVA 

« Hide

References

[1]Brurberg M.
Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
[2]"Chitinase B from Serratia marcescens BJL200 is exported to the periplasm without processing."
Brurberg M.B., Eijsink V.G., Haandrikman A.J., Venema G., Nes I.F.
Microbiology 141:123-131(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[3]"Structure of a two-domain chitotriosidase from Serratia marcescens at 1.9-A resolution."
van Aalten D.M., Synstad B., Brurberg M.B., Hough E., Riise B.W., Eijsink V.G., Wierenga R.K.
Proc. Natl. Acad. Sci. U.S.A. 97:5842-5847(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS).
[4]"Structural insights into the catalytic mechanism of a family 18 exo-chitinase."
van Aalten D.M., Komander D., Synstad B., Gaseidnes S., Peter M.G., Eijsink V.G.
Proc. Natl. Acad. Sci. U.S.A. 98:8979-8984(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH N-ACETYL-D-GLUCOSAMINE AND SULFATE.
[5]"Psammaplin A, a chitinase inhibitor isolated from the Fijian marine sponge Aplysinella rhax."
Tabudravu J.N., Eijsink V.G., Gooday G.W., Jaspars M., Komander D., Legg M., Synstad B., van Aalten D.M.
Bioorg. Med. Chem. 10:1123-1128(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEX WITH SULFATE.
[6]"Interactions of a family 18 chitinase with the designed inhibitor HM508 and its degradation product, chitobiono-delta-lactone."
Vaaje-Kolstad G., Vasella A., Peter M.G., Netter C., Houston D.R., Westereng B., Synstad B., Eijsink V.G., van Aalten D.M.
J. Biol. Chem. 279:3612-3619(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 1-498 IN COMPLEX WITH N-ACETYL-D-GLUCOSAMINE AND SULFATE.
[7]"Structure-based exploration of cyclic dipeptide chitinase inhibitors."
Houston D.R., Synstad B., Eijsink V.G., Stark M.J., Eggleston I.M., van Aalten D.M.
J. Med. Chem. 47:5713-5720(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEX WITH SULFATE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z36295 Genomic DNA. Translation: CAA85292.1.
PIRS52422.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1E15X-ray1.90A/B1-499[»]
1E6PX-ray1.70A/B1-499[»]
1E6RX-ray2.50A/B1-499[»]
1E6ZX-ray1.99A/B2-499[»]
1GPFX-ray1.85A/B1-499[»]
1UR8X-ray1.90A/B1-499[»]
1UR9X-ray1.80A/B1-498[»]
1W1PX-ray2.10A/B1-499[»]
1W1TX-ray1.90A/B1-499[»]
1W1VX-ray1.85A/B1-499[»]
1W1YX-ray1.85A/B1-499[»]
ProteinModelPortalQ54276.
SMRQ54276. Positions 2-499.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM5. Carbohydrate-Binding Module Family 5.
GH18. Glycoside Hydrolase Family 18.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.10.10.20. 1 hit.
3.20.20.80. 2 hits.
InterProIPR003610. CBM_fam5/12.
IPR011583. Chitinase_II.
IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02839. CBM_5_12. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTSM00495. ChtBD3. 1 hit.
SM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMSSF51055. SSF51055. 1 hit.
SSF51445. SSF51445. 2 hits.
PROSITEPS01095. CHITINASE_18. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ54276.

Entry information

Entry nameQ54276_SERMA
AccessionPrimary (citable) accession number: Q54276
Entry history
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: December 11, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)