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Protein

Isopenicillin N synthase

Gene

pcbC

Organism
Streptomyces griseus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin.

Catalytic activityi

N-((5S)-5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: penicillin G biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Isopenicillin N synthase (pcbC)
  3. no protein annotated in this organism
This subpathway is part of the pathway penicillin G biosynthesis, which is itself part of Antibiotic biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine, the pathway penicillin G biosynthesis and in Antibiotic biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi212 – 2121IronPROSITE-ProRule annotation
Metal bindingi214 – 2141IronPROSITE-ProRule annotation
Metal bindingi268 – 2681IronPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic biosynthesis

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

UniPathwayiUPA00149; UER00240.

Names & Taxonomyi

Protein namesi
Recommended name:
Isopenicillin N synthase (EC:1.21.3.1)
Short name:
IPNS
Gene namesi
Name:pcbC
OrganismiStreptomyces griseus
Taxonomic identifieri1911 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329Isopenicillin N synthasePRO_0000219506Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ54243.
SMRiQ54243. Positions 5-329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini180 – 286107Fe2OG dioxygenasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002057. Isopenicillin-N_synth_CS.
IPR002283. Isopenicillin-N_synthase.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PRINTSiPR00682. IPNSYNTHASE.
PROSITEiPS51471. FE2OG_OXY. 1 hit.
PS00185. IPNS_1. 1 hit.
PS00186. IPNS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54243-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIPMLPAHV PTIDISPLSG GDADDKKRVA QEINKACRES GFFYASHHGI
60 70 80 90 100
DVQLLKDVVN EFHRTMTDEE KYDLAINAYN KNNPRTRNGY YMAVKGKKAV
110 120 130 140 150
ESWCYLNPSF SEDHPQIRSG TPMHEGNIWP DEKRHQRFRP FCEQYYRDVF
160 170 180 190 200
SLSKVLMRGF ALALGKPEDF FDASLSLADT LSAVTLIHYP YLEDYPPVKT
210 220 230 240 250
GPDGTKLSFE DHLDVSMITV LFQTEVQNLQ VETADGWQDL PTSGENFLVN
260 270 280 290 300
CGTYMGYLTN DYFPAPNHRV KFINAERLSL PFFLHAGHTT VMEPFSPEDT
310 320
RGKELNPPVR YGDYLQQASN ALIAKNGQT
Length:329
Mass (Da):37,368
Last modified:November 1, 1996 - v1
Checksum:i0CD96C8F7CF5A7EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54609 Genomic DNA. Translation: CAA38431.1.
PIRiA61155.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54609 Genomic DNA. Translation: CAA38431.1.
PIRiA61155.

3D structure databases

ProteinModelPortaliQ54243.
SMRiQ54243. Positions 5-329.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00149; UER00240.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002057. Isopenicillin-N_synth_CS.
IPR002283. Isopenicillin-N_synthase.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PRINTSiPR00682. IPNSYNTHASE.
PROSITEiPS51471. FE2OG_OXY. 1 hit.
PS00185. IPNS_1. 1 hit.
PS00186. IPNS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of the isopenicillin N synthase gene of Streptomyces griseus NRRL 3851 and studies of expression and complementation of the cephamycin pathway in Streptomyces clavuligerus."
    Garcia-Dominguea M., Liras P., Martin J.F.
    Antimicrob. Agents Chemother. 35:44-52(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NBRC 13304 / NRRL 3851 / KCC S-1012 / MA-2837.

Entry informationi

Entry nameiIPNS_STRGR
AccessioniPrimary (citable) accession number: Q54243
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: November 11, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.