Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine protease pic autotransporter

Gene

pic

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in intestinal colonization, displays in vitro mucinolytic activity, serum resistance, and hemagglutination. Important to penetrate the intestinal mucus layer.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei127 – 1271Charge relay systemPROSITE-ProRule annotation
Active sitei155 – 1551Charge relay systemPROSITE-ProRule annotation
Active sitei258 – 2581Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Virulence

Protein family/group databases

MEROPSiS06.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease pic autotransporter (EC:3.4.21.-)
Alternative name(s):
ShMu
Cleaved into the following 2 chains:
Gene namesi
Name:pic
Synonyms:she, sigA
Ordered Locus Names:SF2973, S3178
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

Subcellular locationi

Serine protease pic translocator :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 5555Sequence analysisAdd
BLAST
Chaini56 – 13721317Serine protease pic autotransporterPRO_0000387607Add
BLAST
Chaini56 – 10951040Serine protease picPRO_0000026976Add
BLAST
Chaini1096 – 1372277Serine protease pic translocatorSequence analysisPRO_0000026977Add
BLAST

Post-translational modificationi

Cleaved to release the mature protein from the outer membrane.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei1095 – 10962CleavageSequence analysis

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiQ54151.

Interactioni

Protein-protein interaction databases

STRINGi198214.SF2973.

Structurei

3D structure databases

ProteinModelPortaliQ54151.
SMRiQ54151. Positions 1096-1372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini56 – 301246Peptidase S6PROSITE-ProRule annotationAdd
BLAST
Domaini1106 – 1372267AutotransporterPROSITE-ProRule annotationAdd
BLAST

Domaini

The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage (By similarity).By similarity

Sequence similaritiesi

Contains 1 autotransporter (TC 1.B.12) domain. [View classification]PROSITE-ProRule annotation
Contains 1 peptidase S6 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105I1J. Bacteria.
COG3468. LUCA.
HOGENOMiHOG000117212.
KOiK12684.

Family and domain databases

Gene3Di2.160.20.20. 3 hits.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR024973. ESPR.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR000710. Peptidase_S6.
IPR030396. Peptidase_S6_dom.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF13018. ESPR. 1 hit.
PF02395. Peptidase_S6. 1 hit.
[Graphical view]
PRINTSiPR00921. IGASERPTASE.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 3 hits.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS51691. PEPTIDASE_S6. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54151-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKVYSLKYC PVTGGLIAVS ELARRVIKKT CRRLTHILLA GIPAICLCYS
60 70 80 90 100
QISQAGIVRS DIAYQIYRDF AENKGLFVPG ANDIPVYDKD GKLVGRLGKA
110 120 130 140 150
PMADFSSVSS NGVATLVSPQ YIVSVKHNGG YRSVSFGNGK NTYSLVDRNN
160 170 180 190 200
HPSIDFHAPR LNKLVTEVIP SAVTSEGTKA NAYKYTERYT AFYRVGSGTQ
210 220 230 240 250
YTKDKDGNLV KVAGGYAFKT GGTTGVPLIS DATIVSNPGQ TYNPVNGPLP
260 270 280 290 300
DYGAPGDSGS PLFAYDKQQK KWVIVAVLRA YAGINGATNW WNVIPTDYLN
310 320 330 340 350
QVMQDDFDAP VDFVSGLGPL NWTYDKTSGT GTLSQGSKNW TMHGQKDNDL
360 370 380 390 400
NAGKNLVFSG QNGAIILKDS VTQGAGYLEF KDSYTVSAES GKTWTGAGII
410 420 430 440 450
TDKGTNVTWK VNGVAGDNLH KLGEGTLTIN GTGVNPGGLK TGDGIVVLNQ
460 470 480 490 500
QADTAGNIQA FSSVNLASGR PTVVLGDARQ VNPDNISWGY RGGKLDLNGN
510 520 530 540 550
AVTFTRLQAA DYGAVITNNA QQKSQLLLDL KAQDTNVSEP TIGNISPFGG
560 570 580 590 600
TGTPGNLYSM ILNSQTRFYI LKSASYGNTL WGNSLNDPAQ WEFVGMNKNK
610 620 630 640 650
AVQTVKDRIL AGRAKQPVIF HGQLTGNMDV AIPQVPGGRK VIFDGSVNLP
660 670 680 690 700
EGTLSQDSGT LIFQGHPVIH ASISGSAPVS LNQKDWENRQ FTMKTLSLKD
710 720 730 740 750
ADFHLSRNAS LNSDIKSDNS HITLGSDRAF VDKNDGTGNY VIPEEGTSVP
760 770 780 790 800
DTVNDRSQYE GNITLNHNSA LDIGSRFTGG IDAYDSAVSI TSPDVLLTAP
810 820 830 840 850
GAFAGSSLTV HDGGHLTALN GLFSDGHIQA GKNGKITLSG TPVKDTANQY
860 870 880 890 900
APAVYLTDGY DLTGDNAALE ITRGAHASGD IHASAASTVT IGSDTPAELA
910 920 930 940 950
SAETAASAFA GSLLEGYNAA FNGAITGGRA DVSMHNALWT LGGDSAIHSL
960 970 980 990 1000
TVRNSRISSE GDRTFRTLTV NKLDATGSDF VLRTDLKNAD KINVTEKATG
1010 1020 1030 1040 1050
SDNSLNVSFM NNPAQGQALN IPLVTAPAGT SAEMFKAGTR VTGFSRVTPT
1060 1070 1080 1090 1100
LHVDTSGGNT KWILDGFKAE ADKAAAAKAD SFMNAGYKNF MTEVNNLNKR
1110 1120 1130 1140 1150
MGDLRDTNGD AGAWARIMSG AGSADGGYSD NYTHVQVGFD KKHELDGVDL
1160 1170 1180 1190 1200
FTGVTMTYTD SSADSHAFSG KTKSVGGGLY ASALFESGAY IDLIGKYIHH
1210 1220 1230 1240 1250
DNDYTGNFAS LGTKHYNTHS WYAGAETGYR YHLTEDTFIE PQAELVYGAV
1260 1270 1280 1290 1300
SGKTFRWKDG DMDLSMKNRD FSPLVGRTGV ELGKTFSGKD WSVTARAGTS
1310 1320 1330 1340 1350
WQFDLLNNGE TVLRDASGEK RIKGEKDSRM LFNVGMNAQI KDNMRFGLEF
1360 1370
EKSAFGKYNV DNAVNANFRY MF
Length:1,372
Mass (Da):146,453
Last modified:July 19, 2005 - v3
Checksum:iAE47B3C621A61F67
GO

Sequence cautioni

The sequence AAK00464 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAN44454 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAP18274 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti354 – 3541K → E in strain: M4243 and YSH6000T.
Natural varianti385 – 3851T → P in strain: M4243 and YSH6000T.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35656 Genomic DNA. Translation: AAB58244.1.
AF200692 Genomic DNA. Translation: AAK00464.1. Different initiation.
AE005674 Genomic DNA. Translation: AAN44454.2. Different initiation.
AE014073 Genomic DNA. Translation: AAP18274.1. Different initiation.
RefSeqiWP_001045650.1. NZ_KL503844.1.

Genome annotation databases

EnsemblBacteriaiAAN44454; AAN44454; SF2973.
AAP18274; AAP18274; S3178.
KEGGisfx:S3178.
PATRICi18708061. VBIShiFle31049_3479.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35656 Genomic DNA. Translation: AAB58244.1.
AF200692 Genomic DNA. Translation: AAK00464.1. Different initiation.
AE005674 Genomic DNA. Translation: AAN44454.2. Different initiation.
AE014073 Genomic DNA. Translation: AAP18274.1. Different initiation.
RefSeqiWP_001045650.1. NZ_KL503844.1.

3D structure databases

ProteinModelPortaliQ54151.
SMRiQ54151. Positions 1096-1372.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198214.SF2973.

Protein family/group databases

MEROPSiS06.005.

Proteomic databases

PaxDbiQ54151.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN44454; AAN44454; SF2973.
AAP18274; AAP18274; S3178.
KEGGisfx:S3178.
PATRICi18708061. VBIShiFle31049_3479.

Phylogenomic databases

eggNOGiENOG4105I1J. Bacteria.
COG3468. LUCA.
HOGENOMiHOG000117212.
KOiK12684.

Family and domain databases

Gene3Di2.160.20.20. 3 hits.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR024973. ESPR.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR000710. Peptidase_S6.
IPR030396. Peptidase_S6_dom.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF13018. ESPR. 1 hit.
PF02395. Peptidase_S6. 1 hit.
[Graphical view]
PRINTSiPR00921. IGASERPTASE.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 3 hits.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS51691. PEPTIDASE_S6. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIC_SHIFL
AccessioniPrimary (citable) accession number: Q54151
Secondary accession number(s): Q7UBM2, Q83Q79, Q9AL58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: September 7, 2016
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The S.flexneri genes encoding ShET1 enterotoxin subunits set1A and set1B are contained within the pic gene, on the complementary non-coding strand.

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.