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Protein

DNA damage-inducible transcript 3 protein

Gene

DDIT3

Organism
Homo sapiens (Human)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

ActivatorUniRule annotation, RepressorUniRule annotation

Keywords - Biological processi

ApoptosisUniRule annotation, Cell cycle, Growth arrestUniRule annotation, Stress responseUniRule annotation, Transcription, Transcription regulationUniRule annotation, Unfolded protein responseUniRule annotation, Wnt signaling pathwayUniRule annotation

Keywords - Ligandi

DNA-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA damage-inducible transcript 3 proteinUniRule annotation
Short name:
DDIT-3UniRule annotation
Alternative name(s):
C/EBP zetaUniRule annotation
C/EBP-homologous proteinUniRule annotation
C/EBP-homologous protein 10UniRule annotation
CCAAT/enhancer-binding protein homologous proteinUniRule annotation
Growth arrest and DNA-damage-inducible protein GADD153UniRule annotation
Gene namesi
Name:DDIT3Imported
ORF Names:hCG_39661Imported
OrganismiHomo sapiens (Human)Imported
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Nucleus UniRule annotation

  • Note: Present in the cytoplasm under non-stressed conditions and ER stress leads to its nuclear accumulation.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation, NucleusUniRule annotation

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27193.

PTM / Processingi

Post-translational modificationi

Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity.UniRule annotation
Ubiquitinated, leading to its degradation by the proteasome.UniRule annotation

Keywords - PTMi

PhosphoproteinUniRule annotation, Ubl conjugationUniRule annotation

Proteomic databases

MaxQBiQ53YD1.

Expressioni

Gene expression databases

BgeeiENSG00000175197.

Interactioni

Subunit structurei

Heterodimer.UniRule annotation

Protein-protein interaction databases

STRINGi9606.ENSP00000447803.

Structurei

3D structure databases

SMRiQ53YD1. Positions 105-149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini99 – 16264BZIP (basic-leucine zipper)InterPro annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili117 – 15842Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the bZIP family.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiENOG410ISXY. Eukaryota.
ENOG41127XB. LUCA.
HOVERGENiHBG051328.
KOiK04452.
PhylomeDBiQ53YD1.

Family and domain databases

InterProiIPR004827. bZIP.
IPR016670. DNA_damage_induc_transcript_3.
[Graphical view]
PANTHERiPTHR16833. PTHR16833. 1 hit.
PfamiPF07716. bZIP_2. 1 hit.
[Graphical view]
PIRSFiPIRSF016571. C/EBPzeta_CHOP_DDIT3. 1 hit.
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q53YD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAESLPFSF GTLSSWELEA WYEDLQEVLS SDENGGTYVS PPGNEEEESK
60 70 80 90 100
IFTTLDPASL AWLTEEEPEP AEVTSTSQSP HSPDSSQSSL AQEEEEEDQG
110 120 130 140 150
RTRKRKQSGH SPARAGKQRM KEKEQENERK VAQLAEENER LKQEIERLTR
160
EVEATRRALI DRMVNLHQA
Length:169
Mass (Da):19,175
Last modified:May 24, 2005 - v1
Checksum:i31905293FB1FBBE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT006691 mRNA. Translation: AAP35337.1.
AK316581 mRNA. Translation: BAG38169.1.
CH471054 Genomic DNA. Translation: EAW97021.1.
CH471054 Genomic DNA. Translation: EAW97022.1.
CH471054 Genomic DNA. Translation: EAW97023.1.
RefSeqiNP_001181985.1. NM_001195056.1.
NP_001181986.1. NM_001195057.1.
NP_004074.2. NM_004083.5.
UniGeneiHs.505777.

Genome annotation databases

GeneIDi1649.
KEGGihsa:1649.
UCSCiuc001soi.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT006691 mRNA. Translation: AAP35337.1.
AK316581 mRNA. Translation: BAG38169.1.
CH471054 Genomic DNA. Translation: EAW97021.1.
CH471054 Genomic DNA. Translation: EAW97022.1.
CH471054 Genomic DNA. Translation: EAW97023.1.
RefSeqiNP_001181985.1. NM_001195056.1.
NP_001181986.1. NM_001195057.1.
NP_004074.2. NM_004083.5.
UniGeneiHs.505777.

3D structure databases

SMRiQ53YD1. Positions 105-149.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000447803.

Proteomic databases

MaxQBiQ53YD1.

Protocols and materials databases

DNASUi1649.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1649.
KEGGihsa:1649.
UCSCiuc001soi.4. human.

Organism-specific databases

CTDi1649.
PharmGKBiPA27193.

Phylogenomic databases

eggNOGiENOG410ISXY. Eukaryota.
ENOG41127XB. LUCA.
HOVERGENiHBG051328.
KOiK04452.
PhylomeDBiQ53YD1.

Miscellaneous databases

ChiTaRSiDDIT3. human.
GenomeRNAii1649.

Gene expression databases

BgeeiENSG00000175197.

Family and domain databases

InterProiIPR004827. bZIP.
IPR016670. DNA_damage_induc_transcript_3.
[Graphical view]
PANTHERiPTHR16833. PTHR16833. 1 hit.
PfamiPF07716. bZIP_2. 1 hit.
[Graphical view]
PIRSFiPIRSF016571. C/EBPzeta_CHOP_DDIT3. 1 hit.
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQ53YD1_HUMAN
AccessioniPrimary (citable) accession number: Q53YD1
Entry historyi
Integrated into UniProtKB/TrEMBL: May 24, 2005
Last sequence update: May 24, 2005
Last modified: September 7, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.