ID   Q53VB3_MUCCI            Unreviewed;       338 AA.
AC   Q53VB3;
DT   24-MAY-2005, integrated into UniProtKB/TrEMBL.
DT   24-MAY-2005, sequence version 1.
DT   03-MAY-2023, entry version 54.
DE   RecName: Full=Cellulase {ECO:0000256|ARBA:ARBA00012601, ECO:0000256|PROSITE-ProRule:PRU10069};
DE            EC=3.2.1.4 {ECO:0000256|ARBA:ARBA00012601, ECO:0000256|PROSITE-ProRule:PRU10069};
GN   Name=mce1 {ECO:0000313|EMBL:BAD95808.1};
OS   Mucor circinelloides (Mucor griseo-roseus).
OC   Eukaryota; Fungi; Fungi incertae sedis; Mucoromycota; Mucoromycotina;
OC   Mucoromycetes; Mucorales; Mucorineae; Mucoraceae; Mucor.
OX   NCBI_TaxID=36080 {ECO:0000313|EMBL:BAD95808.1};
RN   [1] {ECO:0000313|EMBL:BAD95808.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=FERM P-17446 {ECO:0000313|EMBL:BAD95808.1};
RX   PubMed=15838031; DOI=10.1128/JB.187.9.3045-3051.2005;
RA   Baba Y., Shimonaka A., Koga J., Kubota H., Kono T.;
RT   "Alternative splicing produces two endoglucanases with one or two
RT   carbohydrate-binding modules in Mucor circinelloides.";
RL   J. Bacteriol. 187:3045-3051(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00000966, ECO:0000256|PROSITE-
CC         ProRule:PRU10069};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 45 (cellulase K) family.
CC       {ECO:0000256|ARBA:ARBA00007793}.
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DR   EMBL; AB175927; BAD95808.1; -; mRNA.
DR   AlphaFoldDB; Q53VB3; -.
DR   SMR; Q53VB3; -.
DR   CAZy; CBM1; Carbohydrate-Binding Module Family 1.
DR   CAZy; GH45; Glycoside Hydrolase Family 45.
DR   CLAE; EGL45A1_MUCCI; -.
DR   VEuPathDB; FungiDB:HMPREF1544_05234; -.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030248; F:cellulose binding; IEA:InterPro.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.40.10; RlpA-like domain; 1.
DR   InterPro; IPR035971; CBD_sf.
DR   InterPro; IPR000254; Cellulose-bd_dom_fun.
DR   InterPro; IPR000334; Glyco_hydro_45.
DR   InterPro; IPR036908; RlpA-like_sf.
DR   PANTHER; PTHR39730; ENDOGLUCANASE 1; 1.
DR   PANTHER; PTHR39730:SF1; ENDOGLUCANASE 1; 1.
DR   Pfam; PF00734; CBM_1; 1.
DR   Pfam; PF02015; Glyco_hydro_45; 1.
DR   SMART; SM00236; fCBD; 1.
DR   SUPFAM; SSF50685; Barwin-like endoglucanases; 1.
DR   SUPFAM; SSF57180; Cellulose-binding domain; 1.
DR   PROSITE; PS00562; CBM1_1; 1.
DR   PROSITE; PS51164; CBM1_2; 1.
DR   PROSITE; PS01140; GLYCOSYL_HYDROL_F45; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW   Cellulose degradation {ECO:0000256|ARBA:ARBA00023001};
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326};
KW   Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           23..338
FT                   /note="Cellulase"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5004249039"
FT   DOMAIN          24..62
FT                   /note="CBM1"
FT                   /evidence="ECO:0000259|PROSITE:PS51164"
FT   ACT_SITE        144
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10069"
SQ   SEQUENCE   338 AA;  34333 MW;  3F4A4B830D4D7185 CRC64;
     MKFTVAITSI AVALALSSSA EAASCSSVYG QCGGIGWSGP TCCESGSTCV AQEGNKYYSQ
     CLPGSHSNNA GNASSTKKTS TKTSTTTAKA TATVTTKTVT KTTTKTTTKT STTAAASTST
     SSSAGYKVIS GGKSGSGSTT RYWDCCKASC SWPGKASVTG PVDTCASNGI SLLDANAQSG
     CNGGNGFMCN NNQPWAVNDE LAYGFAAASI AGSNEAGWCC GCYELTFTSG AASGKKMVVQ
     VTNTGGDLGS NHFDLQMPGG GVGIFNGCAA QWGAPNDGWG ARYGGVSSVS DCASLPSALQ
     AGCKWRFNWF KNSDNPTMTF KEVTCPAELT TRSGCERK
//