Q53T94 (TAF1B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
TATA box-binding protein-associated factor RNA polymerase I subunit B

Alternative name(s):
RNA polymerase I-specific TBP-associated factor 63 kDa
Short name=TAFI63
TATA box-binding protein-associated factor 1B
Short name=TBP-associated factor 1B
Transcription initiation factor SL1/TIF-IB subunit B

Gene names

Name:

TAF1B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during trancription initiation such as preinitiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. Binds rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex and, possibly, directly through its interaction with RRN3. Ref.1 Ref.5 Ref.6 Ref.9 Ref.11 Ref.12
Subunit structure	Interacts with FLNA (via N-terminus) By similarity. Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B, TAF1C and TAF1D. In the complex interacts directly with TBP, TAF1A and TAF1C. Interaction of the SL1/TIF-IB subunits with TBP excludes interaction of TBP with the transcription factor IID (TFIID) subunits. Interacts with TBP and RRN3. Ref.1 Ref.8
Subcellular location	Nucleus › nucleolus.
Domain	Although it shares weak sequence similarity with GTF2B/TFIIB, displays a similar subdomain organization as GTF2B/TFIIB, with a N-terminal zinc finger, a connecting region (composed of B-reader and B-linker regions), followed by 2 cyclin folds. The RRN7-type zinc finger plays an essential postrecruitment role in Pol I transcription at a step preceding synthesis of the first 40 nucleotides (Ref.11 and Ref.12).
Sequence similarities	Belongs to the RRN7/TAF1B family. Contains 1 RRN7-type zinc finger.
Sequence caution	The sequence AAA62863.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	termination of RNA polymerase I transcription Traceable author statement. Source: Reactome transcription elongation from RNA polymerase I promoter Traceable author statement. Source: Reactome
Cellular_component	RNA polymerase I core factor complex Inferred from direct assay Ref.11Ref.12. Source: UniProtKB
Molecular_function	RNA polymerase I CORE element sequence-specific DNA binding Inferred from direct assay Ref.11Ref.12. Source: UniProtKB RNA polymerase I CORE element sequence-specific DNA binding transcription factor recruiting transcription factor activity Inferred from direct assay Ref.11Ref.12. Source: UniProtKB TBP-class protein binding Traceable author statement Ref.12. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q53T94-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q53T94-2)

The sequence of this isoform differs from the canonical sequence as follows:
     523-588: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q53T94-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-255: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 588	588	TATA box-binding protein-associated factor RNA polymerase I subunit B		PRO_0000261392
Regions
	Zinc finger	4 – 39	36	RRN7-type
	Region	40 – 68	29	B-reader
	Region	69 – 73	5	B-linker
	Region	74 – 261	188	N-terminal cyclin fold
	Region	262 – 372	111	C-terminal cyclin fold
Sites
	Metal binding	13	1	Zinc Probable
	Metal binding	16	1	Zinc Probable
	Metal binding	31	1	Zinc Probable
	Metal binding	34	1	Zinc Probable
Amino acid modifications
	Modified residue	440	1	N6-acetyllysine Ref.10
Natural variations
	Alternative sequence	1 – 255	255	Missing in isoform 3.		VSP_042954
	Alternative sequence	523 – 588	66	Missing in isoform 2.		VSP_021677
	Natural variant	6	1	A → S. Ref.1 Ref.4 Corresponds to variant rs2303914 [ dbSNP | Ensembl ].		VAR_029378
	Natural variant	282	1	V → I. Ref.1 Ref.2 Ref.4 Corresponds to variant rs396190 [ dbSNP | Ensembl ].		VAR_029379
	Natural variant	292	1	R → H. Corresponds to variant rs16867223 [ dbSNP | Ensembl ].		VAR_057260
	Natural variant	351	1	T → A. Ref.1 Ref.2 Ref.4 Corresponds to variant rs1054565 [ dbSNP | Ensembl ].		VAR_029380
	Natural variant	462	1	E → D. Ref.1 Ref.2 Ref.4 Corresponds to variant rs1820965 [ dbSNP | Ensembl ].		VAR_029381
	Natural variant	487	1	T → M. Corresponds to variant rs16867245 [ dbSNP | Ensembl ].		VAR_029382
Experimental info
	Mutagenesis	13	1	C → A: Abolishes Pol I transcription but not recruitment of SL1/TIF-IB complex to rDNA promoters. Ref.12
	Mutagenesis	31	1	C → A: Abolishes Pol I transcription but not recruitment of SL1/TIF-IB complex to rDNA promoters. Ref.12
	Mutagenesis	34	1	C → A: Abolishes Pol I transcription but not recruitment of SL1/TIF-IB complex to rDNA promoters. Ref.12
	Sequence conflict	1	1	M → L in AAA62863. Ref.1
	Sequence conflict	372	1	L → M in AAA62863. Ref.1
	Sequence conflict	522	1	C → W in AAA62863. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: CE45DE622E28FD6E	FASTA	588	68,832
	10 20 30 40 50 60 MDLEEAEEFK ERCTQCAAVS WGLTDEGKYY CTSCHNVTER YQEVTNTDLI PNTQIKALNR 70 80 90 100 110 120 GLKKKNNTEK GWDWYVCEGF QYILYQQAEA LKNLGVGPEL KNDVLHNFWK RYLQKSKQAY 130 140 150 160 170 180 CKNPVYTTGR KPTVLEDNLS HSDWASEPEL LSDVSCPPFL ESGAESQSDI HTRKPFPVSK 190 200 210 220 230 240 ASQSETSVCS GSLDGVEYSQ RKEKGIVKMT MPQTLAFCYL SLLWQREAIT LSDLLRFVEE 250 260 270 280 290 300 DHIPYINAFQ HFPEQMKLYG RDRGIFGIES WPDYEDIYKK TVEVGTFLDL PRFPDITEDC 310 320 330 340 350 360 YLHPNILCMK YLMEVNLPDE MHSLTCHVVK MTGMGEVDFL TFDPIAKMAK TVKYDVQAVA 370 380 390 400 410 420 IIVVVLKLLF LLDDSFEWSL SNLAEKHNEK NKKDKPWFDF RKWYQIMKKA FDEKKQKWEE 430 440 450 460 470 480 ARAKYLWKSE KPLYYSFVDK PVAYKKREMV VNLQKQFSTL VESTATAGKK SPSSFQFNWT 490 500 510 520 530 540 EEDTDRTCFH GHSLQGVLKE KGQSLLTKNS LYWLSTQKFC RCYCTHVTTY EESNYSLSYQ 550 560 570 580 FILNLFSFLL RIKTSLLHEE VSLVEKKLFE KKYSVKRKKS RSKKVRRH
	Isoform 2 [UniParc]. Checksum: A0277EAF25B4F50F	FASTA	522	60,790
	10 20 30 40 50 60 MDLEEAEEFK ERCTQCAAVS WGLTDEGKYY CTSCHNVTER YQEVTNTDLI PNTQIKALNR 70 80 90 100 110 120 GLKKKNNTEK GWDWYVCEGF QYILYQQAEA LKNLGVGPEL KNDVLHNFWK RYLQKSKQAY 130 140 150 160 170 180 CKNPVYTTGR KPTVLEDNLS HSDWASEPEL LSDVSCPPFL ESGAESQSDI HTRKPFPVSK 190 200 210 220 230 240 ASQSETSVCS GSLDGVEYSQ RKEKGIVKMT MPQTLAFCYL SLLWQREAIT LSDLLRFVEE 250 260 270 280 290 300 DHIPYINAFQ HFPEQMKLYG RDRGIFGIES WPDYEDIYKK TVEVGTFLDL PRFPDITEDC 310 320 330 340 350 360 YLHPNILCMK YLMEVNLPDE MHSLTCHVVK MTGMGEVDFL TFDPIAKMAK TVKYDVQAVA 370 380 390 400 410 420 IIVVVLKLLF LLDDSFEWSL SNLAEKHNEK NKKDKPWFDF RKWYQIMKKA FDEKKQKWEE 430 440 450 460 470 480 ARAKYLWKSE KPLYYSFVDK PVAYKKREMV VNLQKQFSTL VESTATAGKK SPSSFQFNWT 490 500 510 520 EEDTDRTCFH GHSLQGVLKE KGQSLLTKNS LYWLSTQKFC RC
	Isoform 3 [UniParc]. Checksum: 6E716737AB58736B	FASTA	333	39,533
	10 20 30 40 50 60 MKLYGRDRGI FGIESWPDYE DIYKKTVEVG TFLDLPRFPD ITEDCYLHPN ILCMKYLMEV 70 80 90 100 110 120 NLPDEMHSLT CHVVKMTGMG EVDFLTFDPI AKMAKTVKYD VQAVAIIVVV LKLLFLLDDS 130 140 150 160 170 180 FEWSLSNLAE KHNEKNKKDK PWFDFRKWYQ IMKKAFDEKK QKWEEARAKY LWKSEKPLYY 190 200 210 220 230 240 SFVDKPVAYK KREMVVNLQK QFSTLVESTA TAGKKSPSSF QFNWTEEDTD RTCFHGHSLQ 250 260 270 280 290 300 GVLKEKGQSL LTKNSLYWLS TQKFCRCYCT HVTTYEESNY SLSYQFILNL FSFLLRIKTS 310 320 330 LLHEEVSLVE KKLFEKKYSV KRKKSRSKKV RRH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Reconstitution of transcription factor SL1: exclusive binding of TBP by SL1 or TFIID subunits." Comai L., Zomerdijk J.C.B.M., Beckmann H., Zhou S., Admon A., Tjian R. Science 266:1966-1972(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 11-20; 42-56; 93-110; 123-135; 264-276; 281-289; 311-321; 331-340; 396-407; 429-444; 448-466 AND 471-480, FUNCTION, INTERACTION WITH TBP; TAF1A AND TAF1C, VARIANTS SER-6; ILE-282; ALA-351 AND ASP-462.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANTS ILE-282; ALA-351 AND ASP-462. Tissue: Corpus callosum.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS SER-6; ILE-282; ALA-351 AND ASP-462. Tissue: Colon.
[5]	"Assembly of transcriptionally active RNA polymerase I initiation factor SL1 from recombinant subunits." Zomerdijk J.C., Beckmann H., Comai L., Tjian R. Science 266:2015-2018(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Coactivator and promoter-selective properties of RNA polymerase I TAFs." Beckmann H., Chen J.L., O'Brien T., Tjian R. Science 270:1506-1509(1995) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DNA-BINDING.
[7]	"Genomic localization of the human genes TAF1A, TAF1B and TAF1C, encoding TAF(I)48, TAF(I)63 and TAF(I)110 subunits of class I general transcription initiation factor SL1." Di Pietro C., Rapisarda A., Amico V., Bonaiuto C., Viola A., Scalia M., Motta S., Amato A., Engel H., Messina A., Sichel G., Grzeschik K., Purrello M. Cytogenet. Cell Genet. 89:133-136(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[8]	"hRRN3 is essential in the SL1-mediated recruitment of RNA polymerase I to rRNA gene promoters." Miller G., Panov K.I., Friedrich J.K., Trinkle-Mulcahy L., Lamond A.I., Zomerdijk J.C.B.M. EMBO J. 20:1373-1382(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RRN3.
[9]	"TBP-TAF complex SL1 directs RNA polymerase I pre-initiation complex formation and stabilizes upstream binding factor at the rDNA promoter." Friedrich J.K., Panov K.I., Cabart P., Russell J., Zomerdijk J.C.B.M. J. Biol. Chem. 280:29551-29558(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE SL1/TIF-IB COMPLEX.
[10]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-440, MASS SPECTROMETRY.
[11]	"Yeast Rrn7 and human TAF1B are TFIIB-related RNA polymerase I general transcription factors." Knutson B.A., Hahn S. Science 333:1637-1640(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"TAF1B is a TFIIB-like component of the basal transcription machinery for RNA polymerase I." Naidu S., Friedrich J.K., Russell J., Zomerdijk J.C. Science 333:1640-1642(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF CYS-13; CYS-31 AND CYS-34.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L39061 mRNA. Translation: AAA62863.1. Different initiation. AK295402 mRNA. Translation: BAG58354.1. AC010969 Genomic DNA. Translation: AAX93272.1. BC018137 mRNA. Translation: AAH18137.1.
IPI	IPI00291416. IPI00807704.
PIR	I61581.
RefSeq	NP_005671.2. NM_005680.2.
UniGene	Hs.584833.
3D structure databases
ProteinModelPortal	Q53T94.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q53T94. 2 interactions.
MINT	MINT-4543781.
STRING	9606.ENSP00000263663.
PTM databases
PhosphoSite	Q53T94.
Polymorphism databases
DMDM	74726856.
Proteomic databases
PaxDb	Q53T94.
PRIDE	Q53T94.
Protocols and materials databases
DNASU	9014.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000263663; ENSP00000263663; ENSG00000115750. ENST00000396242; ENSP00000379542; ENSG00000115750.
GeneID	9014.
KEGG	hsa:9014.
UCSC	uc002qzy.4. human.
Organism-specific databases
CTD	9014.
GeneCards	GC02P009983.
H-InvDB	HIX0001812.
HGNC	HGNC:11533. TAF1B.
MIM	604904. gene.
neXtProt	NX_Q53T94.
PharmGKB	PA36308.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG72502.
HOGENOM	HOG000124571.
HOVERGEN	HBG053463.
InParanoid	Q53T94.
KO	K15213.
OMA	NSLYWLS.
OrthoDB	EOG4CVG6V.
Enzyme and pathway databases
Reactome	REACT_1788. Transcription. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	Q53T94.
Bgee	Q53T94.
CleanEx	HS_TAF1B.
Genevestigator	Q53T94.
GermOnline	ENSG00000115750. Homo sapiens.
Family and domain databases
InterPro	IPR021752. TF_Rrn7. [Graphical view]
Pfam	PF11781. RRN7. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	9014.
NextBio	33771.
SOURCE	Search...

Entry information

Entry name

TAF1B_HUMAN

Accession

Primary (citable) accession number: Q53T94
Secondary accession number(s): B4DI42 , F8WD72, Q15574, Q8WVC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	May 24, 2005
Last modified:	April 3, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.