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Protein

TATA box-binding protein-associated factor RNA polymerase I subunit B

Gene

TAF1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during transcription initiation such as pre-initiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. Binds rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex and, possibly, directly through its interaction with RRN3.6 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi13ZincCurated1
Metal bindingi16ZincCurated1
Metal bindingi31ZincCurated1
Metal bindingi34ZincCurated1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri4 – 39RRN7-typeAdd BLAST36

GO - Molecular functioni

  • DNA binding transcription factor activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase I CORE element sequence-specific DNA binding Source: UniProtKB
  • TBP-class protein binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-427359 SIRT1 negatively regulates rRNA expression
R-HSA-427413 NoRC negatively regulates rRNA expression
R-HSA-5250924 B-WICH complex positively regulates rRNA expression
R-HSA-73762 RNA Polymerase I Transcription Initiation
R-HSA-73772 RNA Polymerase I Promoter Escape
R-HSA-73777 RNA Polymerase I Chain Elongation
R-HSA-73863 RNA Polymerase I Transcription Termination

Names & Taxonomyi

Protein namesi
Recommended name:
TATA box-binding protein-associated factor RNA polymerase I subunit B
Alternative name(s):
RNA polymerase I-specific TBP-associated factor 63 kDa
Short name:
TAFI63
TATA box-binding protein-associated factor 1B
Short name:
TBP-associated factor 1B
Transcription initiation factor SL1/TIF-IB subunit B
Gene namesi
Name:TAF1B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000115750.16
HGNCiHGNC:11533 TAF1B
MIMi604904 gene
neXtProtiNX_Q53T94

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi13C → A: Abolishes Pol I transcription but not recruitment of SL1/TIF-IB complex to rDNA promoters. 1 Publication1
Mutagenesisi31C → A: Abolishes Pol I transcription but not recruitment of SL1/TIF-IB complex to rDNA promoters. 1 Publication1
Mutagenesisi34C → A: Abolishes Pol I transcription but not recruitment of SL1/TIF-IB complex to rDNA promoters. 1 Publication1

Organism-specific databases

DisGeNETi9014
OpenTargetsiENSG00000115750
PharmGKBiPA36308

Polymorphism and mutation databases

BioMutaiTAF1B
DMDMi74726856

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002613921 – 588TATA box-binding protein-associated factor RNA polymerase I subunit BAdd BLAST588

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei440N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ53T94
MaxQBiQ53T94
PaxDbiQ53T94
PeptideAtlasiQ53T94
PRIDEiQ53T94

PTM databases

iPTMnetiQ53T94
PhosphoSitePlusiQ53T94

Expressioni

Gene expression databases

BgeeiENSG00000115750
CleanExiHS_TAF1B
ExpressionAtlasiQ53T94 baseline and differential
GenevisibleiQ53T94 HS

Organism-specific databases

HPAiHPA071254

Interactioni

Subunit structurei

Interacts with FLNA (via N-terminus) (By similarity). Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B, TAF1C and TAF1D. In the complex interacts directly with TBP, TAF1A and TAF1C. Interaction of the SL1/TIF-IB subunits with TBP excludes interaction of TBP with the transcription factor IID (TFIID) subunits. Interacts with TBP and RRN3.By similarity2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • TBP-class protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi114483, 48 interactors
CORUMiQ53T94
IntActiQ53T94, 15 interactors
MINTiQ53T94
STRINGi9606.ENSP00000263663

Structurei

3D structure databases

ProteinModelPortaliQ53T94
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni40 – 68B-readerAdd BLAST29
Regioni69 – 73B-linker5
Regioni74 – 261N-terminal cyclin foldAdd BLAST188
Regioni262 – 372C-terminal cyclin foldAdd BLAST111

Domaini

Although it shares weak sequence similarity with GTF2B/TFIIB, displays a similar subdomain organization as GTF2B/TFIIB, with a N-terminal zinc finger, a connecting region (composed of B-reader and B-linker regions), followed by 2 cyclin folds. The RRN7-type zinc finger plays an essential postrecruitment role in Pol I transcription at a step preceding synthesis of the first 40 nucleotides (PubMed:21921198 and PubMed:21921199).

Sequence similaritiesi

Belongs to the RRN7/TAF1B family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri4 – 39RRN7-typeAdd BLAST36

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IKUN Eukaryota
ENOG410YZ64 LUCA
GeneTreeiENSGT00440000033827
HOGENOMiHOG000124571
HOVERGENiHBG053463
InParanoidiQ53T94
KOiK15213
OMAiCMKYLME
OrthoDBiEOG091G0UVY
PhylomeDBiQ53T94
TreeFamiTF324353

Family and domain databases

InterProiView protein in InterPro
IPR033599 TAF1B/Rrn7
IPR021752 TF_Rrn7_Zf
PANTHERiPTHR31576 PTHR31576, 1 hit
PfamiView protein in Pfam
PF11781 zf-RRN7, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q53T94-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLEEAEEFK ERCTQCAAVS WGLTDEGKYY CTSCHNVTER YQEVTNTDLI
60 70 80 90 100
PNTQIKALNR GLKKKNNTEK GWDWYVCEGF QYILYQQAEA LKNLGVGPEL
110 120 130 140 150
KNDVLHNFWK RYLQKSKQAY CKNPVYTTGR KPTVLEDNLS HSDWASEPEL
160 170 180 190 200
LSDVSCPPFL ESGAESQSDI HTRKPFPVSK ASQSETSVCS GSLDGVEYSQ
210 220 230 240 250
RKEKGIVKMT MPQTLAFCYL SLLWQREAIT LSDLLRFVEE DHIPYINAFQ
260 270 280 290 300
HFPEQMKLYG RDRGIFGIES WPDYEDIYKK TVEVGTFLDL PRFPDITEDC
310 320 330 340 350
YLHPNILCMK YLMEVNLPDE MHSLTCHVVK MTGMGEVDFL TFDPIAKMAK
360 370 380 390 400
TVKYDVQAVA IIVVVLKLLF LLDDSFEWSL SNLAEKHNEK NKKDKPWFDF
410 420 430 440 450
RKWYQIMKKA FDEKKQKWEE ARAKYLWKSE KPLYYSFVDK PVAYKKREMV
460 470 480 490 500
VNLQKQFSTL VESTATAGKK SPSSFQFNWT EEDTDRTCFH GHSLQGVLKE
510 520 530 540 550
KGQSLLTKNS LYWLSTQKFC RCYCTHVTTY EESNYSLSYQ FILNLFSFLL
560 570 580
RIKTSLLHEE VSLVEKKLFE KKYSVKRKKS RSKKVRRH
Length:588
Mass (Da):68,832
Last modified:May 24, 2005 - v1
Checksum:iCE45DE622E28FD6E
GO
Isoform 2 (identifier: Q53T94-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     523-588: Missing.

Note: No experimental confirmation available.
Show »
Length:522
Mass (Da):60,790
Checksum:iA0277EAF25B4F50F
GO
Isoform 3 (identifier: Q53T94-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-255: Missing.

Note: No experimental confirmation available.
Show »
Length:333
Mass (Da):39,533
Checksum:i6E716737AB58736B
GO

Sequence cautioni

The sequence AAA62863 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1M → L in AAA62863 (PubMed:7801123).Curated1
Sequence conflicti372L → M in AAA62863 (PubMed:7801123).Curated1
Sequence conflicti522C → W in AAA62863 (PubMed:7801123).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0293786A → S2 PublicationsCorresponds to variant dbSNP:rs2303914Ensembl.1
Natural variantiVAR_029379282V → I3 PublicationsCorresponds to variant dbSNP:rs396190Ensembl.1
Natural variantiVAR_057260292R → H. Corresponds to variant dbSNP:rs16867223Ensembl.1
Natural variantiVAR_029380351T → A3 PublicationsCorresponds to variant dbSNP:rs1054565Ensembl.1
Natural variantiVAR_029381462E → D3 PublicationsCorresponds to variant dbSNP:rs1820965Ensembl.1
Natural variantiVAR_029382487T → M. Corresponds to variant dbSNP:rs16867245Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0429541 – 255Missing in isoform 3. 1 PublicationAdd BLAST255
Alternative sequenceiVSP_021677523 – 588Missing in isoform 2. CuratedAdd BLAST66

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L39061 mRNA Translation: AAA62863.1 Different initiation.
AK295402 mRNA Translation: BAG58354.1
AC010969 Genomic DNA Translation: AAX93272.1
BC018137 mRNA Translation: AAH18137.1
CCDSiCCDS33143.1 [Q53T94-1]
PIRiI61581
RefSeqiNP_001305905.1, NM_001318976.1
NP_005671.2, NM_005680.2
UniGeneiHs.584833

Genome annotation databases

EnsembliENST00000263663; ENSP00000263663; ENSG00000115750 [Q53T94-1]
GeneIDi9014
KEGGihsa:9014
UCSCiuc002qzz.4 human [Q53T94-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTAF1B_HUMAN
AccessioniPrimary (citable) accession number: Q53T94
Secondary accession number(s): B4DI42
, F8WD72, Q15574, Q8WVC3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: May 24, 2005
Last modified: May 23, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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