Reviewed, UniProtKB/Swiss-Prot Q53T94 (TAF1B_HUMAN)

Names and origin

Protein names

Recommended name:
    TATA box-binding protein-associated factor RNA polymerase I subunit B
Alternative name(s):
    TATA box-binding protein-associated factor 1B
      Short name=TBP-associated factor 1B
    TBP-associated factor RNA polymerase I 63 kDa
      Short name=TAFI63
    Transcription factor SL1

Gene names

Name:

TAF1B

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the transcription factor SL1/TIFIB complex involved in the assembly of the PIC (preinitiation complex). Ref.1
Subunit structure	Component of the transcription factor SL1/TIFIB complex which is composed of TBP and at least TAF1A, TAF1B and TAF1C. In the complex, interacts directly with TBP, TAF1A and TAF1C. Interaction of the SL1/TIFIB subunits with TBP excludes interaction with the transcription factor IID (TFIID) subunits. This mutually exclusive binding directs the formation of promoter and RNA polymerase-selective TBP-TAF complexes. Ref.1
Subcellular location	Nucleus.
Sequence similarities	Belongs to the TAF1 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	DNA-binding
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Ref.1 Non-traceable author statement. Source: UniProtKB
Cellular component	nucleoplasm Inferred from Experiment. Source: Reactome
Molecular function	transcription factor activity Ref.1 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q53T94-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q53T94-2)

The sequence of this isoform differs from the canonical sequence as follows:
     523-588: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 588	588	TATA box-binding protein-associated factor RNA polymerase I subunit B		PRO_0000261392
Natural variations
	Alternative sequence	523 – 588	66	Missing in isoform 2.		VSP_021677
	Natural variant	6	1	A → S: dbSNP rs2303914. Ref.1 Ref.3		VAR_029378
	Natural variant	282	1	V → I: dbSNP rs396190. Ref.1 Ref.3		VAR_029379
	Natural variant	292	1	R → H: dbSNP rs16867223.		VAR_057260
	Natural variant	351	1	T → A: dbSNP rs1054565. Ref.1 Ref.3		VAR_029380
	Natural variant	462	1	E → D: dbSNP rs1820965. Ref.1 Ref.3		VAR_029381
	Natural variant	487	1	T → M: dbSNP rs16867245.		VAR_029382
Experimental info
	Sequence conflict	1	1	M → L in AAA62863. Ref.1
	Sequence conflict	372	1	L → M in AAA62863. Ref.1
	Sequence conflict	522	1	C → W in AAA62863. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: CE45DE622E28FD6E	FASTA	588	68,832
	10 20 30 40 50 60 MDLEEAEEFK ERCTQCAAVS WGLTDEGKYY CTSCHNVTER YQEVTNTDLI PNTQIKALNR 70 80 90 100 110 120 GLKKKNNTEK GWDWYVCEGF QYILYQQAEA LKNLGVGPEL KNDVLHNFWK RYLQKSKQAY 130 140 150 160 170 180 CKNPVYTTGR KPTVLEDNLS HSDWASEPEL LSDVSCPPFL ESGAESQSDI HTRKPFPVSK 190 200 210 220 230 240 ASQSETSVCS GSLDGVEYSQ RKEKGIVKMT MPQTLAFCYL SLLWQREAIT LSDLLRFVEE 250 260 270 280 290 300 DHIPYINAFQ HFPEQMKLYG RDRGIFGIES WPDYEDIYKK TVEVGTFLDL PRFPDITEDC 310 320 330 340 350 360 YLHPNILCMK YLMEVNLPDE MHSLTCHVVK MTGMGEVDFL TFDPIAKMAK TVKYDVQAVA 370 380 390 400 410 420 IIVVVLKLLF LLDDSFEWSL SNLAEKHNEK NKKDKPWFDF RKWYQIMKKA FDEKKQKWEE 430 440 450 460 470 480 ARAKYLWKSE KPLYYSFVDK PVAYKKREMV VNLQKQFSTL VESTATAGKK SPSSFQFNWT 490 500 510 520 530 540 EEDTDRTCFH GHSLQGVLKE KGQSLLTKNS LYWLSTQKFC RCYCTHVTTY EESNYSLSYQ 550 560 570 580 FILNLFSFLL RIKTSLLHEE VSLVEKKLFE KKYSVKRKKS RSKKVRRH
	Isoform 2. Checksum: A0277EAF25B4F50F	FASTA	522	60,790
	10 20 30 40 50 60 MDLEEAEEFK ERCTQCAAVS WGLTDEGKYY CTSCHNVTER YQEVTNTDLI PNTQIKALNR 70 80 90 100 110 120 GLKKKNNTEK GWDWYVCEGF QYILYQQAEA LKNLGVGPEL KNDVLHNFWK RYLQKSKQAY 130 140 150 160 170 180 CKNPVYTTGR KPTVLEDNLS HSDWASEPEL LSDVSCPPFL ESGAESQSDI HTRKPFPVSK 190 200 210 220 230 240 ASQSETSVCS GSLDGVEYSQ RKEKGIVKMT MPQTLAFCYL SLLWQREAIT LSDLLRFVEE 250 260 270 280 290 300 DHIPYINAFQ HFPEQMKLYG RDRGIFGIES WPDYEDIYKK TVEVGTFLDL PRFPDITEDC 310 320 330 340 350 360 YLHPNILCMK YLMEVNLPDE MHSLTCHVVK MTGMGEVDFL TFDPIAKMAK TVKYDVQAVA 370 380 390 400 410 420 IIVVVLKLLF LLDDSFEWSL SNLAEKHNEK NKKDKPWFDF RKWYQIMKKA FDEKKQKWEE 430 440 450 460 470 480 ARAKYLWKSE KPLYYSFVDK PVAYKKREMV VNLQKQFSTL VESTATAGKK SPSSFQFNWT 490 500 510 520 EEDTDRTCFH GHSLQGVLKE KGQSLLTKNS LYWLSTQKFC RC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Reconstitution of transcription factor SL1: exclusive binding of TBP by SL1 or TFIID subunits." Comai L., Zomerdijk J.C.B.M., Beckmann H., Zhou S., Admon A., Tjian R. Science 266:1966-1972(1994) [PubMed: 7801123] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 11-20; 42-56; 93-110; 123-135; 264-276; 281-289; 311-321; 331-340; 396-407; 429-444; 448-466 AND 471-480, FUNCTION, INTERACTION WITH TBP; TAF1A AND TAF1C, VARIANTS SER-6; ILE-282; ALA-351 AND ASP-462.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS SER-6; ILE-282; ALA-351 AND ASP-462. Tissue: Colon.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L39061 mRNA. Translation: AAA62863.1. Different initiation. AC010969 Genomic DNA. Translation: AAX93272.1. BC018137 mRNA. Translation: AAH18137.1.
IPI	IPI00291416. IPI00807704.
PIR	I61581.
RefSeq	NP_005671.2.
UniGene	Hs.654508
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q53T94.
Genome annotation databases
Ensembl	ENSG00000115750. Homo sapiens. [Contig view]
GeneID	9014.
Organism-specific databases
GeneCards	GC02P009934.
HGNC	HGNC:11533. TAF1B.
MIM	604904. gene.
PharmGKB	PA36308.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q53T94.
OMA	Q53T94. PRFPDIT.
Enzyme and pathway databases
Reactome	REACT_1788. Transcription.
Gene expression databases
ArrayExpress	Q53T94.
Bgee	Q53T94.
CleanEx	HS_TAF1B.
GermOnline	ENSG00000115750. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	33771.
SOURCE	Search...

Entry information

Entry name

TAF1B_HUMAN

Accession

Primary (citable) accession number: Q53T94
Secondary accession number(s): Q15574, Q8WVC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	May 24, 2005
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)