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Reviewed, UniProtKB/Swiss-Prot Q53T94 (TAF1B_HUMAN)

Last modified June 16, 2009. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    TATA box-binding protein-associated factor RNA polymerase I subunit B
Alternative name(s):
    TATA box-binding protein-associated factor 1B
      Short name=TBP-associated factor 1B
    TBP-associated factor RNA polymerase I 63 kDa
      Short name=TAFI63
    Transcription factor SL1
Gene names
Name: TAF1B
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length588 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Component of the transcription factor SL1/TIFIB complex involved in the assembly of the PIC (preinitiation complex). Ref.1

Subunit structure

Component of the transcription factor SL1/TIFIB complex which is composed of TBP and at least TAF1A, TAF1B and TAF1C. In the complex, interacts directly with TBP, TAF1A and TAF1C. Interaction of the SL1/TIFIB subunits with TBP excludes interaction with the transcription factor IID (TFIID) subunits. This mutually exclusive binding directs the formation of promoter and RNA polymerase-selective TBP-TAF complexes. Ref.1

Subcellular location

Nucleus.

Sequence similarities

Belongs to the TAF1 family.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   LigandDNA-binding
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processregulation of transcription, DNA-dependent

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-dependent Ref.1

Non-traceable author statement. Source: UniProtKB

   Cellular componentnucleoplasm

Inferred from Experiment. Source: Reactome

   Molecular functiontranscription factor activity Ref.1

Non-traceable author statement. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q53T94-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q53T94-2)

The sequence of this isoform differs from the canonical sequence as follows:
     523-588: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 588588TATA box-binding protein-associated factor RNA polymerase I subunit B
PRO_0000261392

Natural variations

Alternative sequence523 – 58866Missing in isoform 2.
VSP_021677
Natural variant61A → S: dbSNP rs2303914. Ref.1 Ref.3
VAR_029378
Natural variant2821V → I: dbSNP rs396190. Ref.1 Ref.3
VAR_029379
Natural variant2921R → H: dbSNP rs16867223.
VAR_057260
Natural variant3511T → A: dbSNP rs1054565. Ref.1 Ref.3
VAR_029380
Natural variant4621E → D: dbSNP rs1820965. Ref.1 Ref.3
VAR_029381
Natural variant4871T → M: dbSNP rs16867245.
VAR_029382

Experimental info

Sequence conflict11M → L in AAA62863. Ref.1
Sequence conflict3721L → M in AAA62863. Ref.1
Sequence conflict5221C → W in AAA62863. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: CE45DE622E28FD6E

FASTA58868,832
        10         20         30         40         50         60 
MDLEEAEEFK ERCTQCAAVS WGLTDEGKYY CTSCHNVTER YQEVTNTDLI PNTQIKALNR 

        70         80         90        100        110        120 
GLKKKNNTEK GWDWYVCEGF QYILYQQAEA LKNLGVGPEL KNDVLHNFWK RYLQKSKQAY 

       130        140        150        160        170        180 
CKNPVYTTGR KPTVLEDNLS HSDWASEPEL LSDVSCPPFL ESGAESQSDI HTRKPFPVSK 

       190        200        210        220        230        240 
ASQSETSVCS GSLDGVEYSQ RKEKGIVKMT MPQTLAFCYL SLLWQREAIT LSDLLRFVEE 

       250        260        270        280        290        300 
DHIPYINAFQ HFPEQMKLYG RDRGIFGIES WPDYEDIYKK TVEVGTFLDL PRFPDITEDC 

       310        320        330        340        350        360 
YLHPNILCMK YLMEVNLPDE MHSLTCHVVK MTGMGEVDFL TFDPIAKMAK TVKYDVQAVA 

       370        380        390        400        410        420 
IIVVVLKLLF LLDDSFEWSL SNLAEKHNEK NKKDKPWFDF RKWYQIMKKA FDEKKQKWEE 

       430        440        450        460        470        480 
ARAKYLWKSE KPLYYSFVDK PVAYKKREMV VNLQKQFSTL VESTATAGKK SPSSFQFNWT 

       490        500        510        520        530        540 
EEDTDRTCFH GHSLQGVLKE KGQSLLTKNS LYWLSTQKFC RCYCTHVTTY EESNYSLSYQ 

       550        560        570        580 
FILNLFSFLL RIKTSLLHEE VSLVEKKLFE KKYSVKRKKS RSKKVRRH 

« Hide

Isoform 2.

Checksum: A0277EAF25B4F50F
Show »

FASTA52260,790

References

« Hide 'large scale' references
[1]"Reconstitution of transcription factor SL1: exclusive binding of TBP by SL1 or TFIID subunits."
Comai L., Zomerdijk J.C.B.M., Beckmann H., Zhou S., Admon A., Tjian R.
Science 266:1966-1972(1994) [PubMed: 7801123] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 11-20; 42-56; 93-110; 123-135; 264-276; 281-289; 311-321; 331-340; 396-407; 429-444; 448-466 AND 471-480, FUNCTION, INTERACTION WITH TBP; TAF1A AND TAF1C, VARIANTS SER-6; ILE-282; ALA-351 AND ASP-462.
[2]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed: 15815621] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS SER-6; ILE-282; ALA-351 AND ASP-462.
Tissue: Colon.

Cross-references

Sequence databases

L39061 mRNA. Translation: AAA62863.1. Different initiation.
AC010969 Genomic DNA. Translation: AAX93272.1.
BC018137 mRNA. Translation: AAH18137.1.
IPIIPI00291416.
IPI00807704.
PIRI61581.
RefSeqNP_005671.2.
UniGeneHs.654508

3D structure databases

ModBaseSearch...

Proteomic databases

PRIDEQ53T94.

Genome annotation databases

EnsemblENSG00000115750. Homo sapiens. [Contig view]
GeneID9014.

Organism-specific databases

GeneCardsGC02P009934.
HGNCHGNC:11533. TAF1B.
MIM604904. gene.
PharmGKBPA36308.
GenAtlasSearch...

Phylogenomic databases

HOVERGENQ53T94.
OMAQ53T94. PRFPDIT.

Enzyme and pathway databases

ReactomeREACT_1788. Transcription.

Gene expression databases

ArrayExpressQ53T94.
BgeeQ53T94.
CleanExHS_TAF1B.
GermOnlineENSG00000115750. Homo sapiens.

Family and domain databases

ProtoNetSearch...

Other Resources

NextBio33771.
SOURCESearch...

Entry information

Entry nameTAF1B_HUMAN
AccessionPrimary (citable) accession number: Q53T94
Secondary accession number(s): Q15574, Q8WVC3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: May 24, 2005
Last modified: June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents