Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q53RT3 (APRV1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Retroviral-like aspartic protease 1

EC=3.4.23.-
Alternative name(s):
Skin-specific retroviral-like aspartic protease
Short name=SASPase
Short name=Skin aspartic protease
TPA-inducible aspartic proteinase-like protein
Short name=TAPS
Gene names
Name:ASPRV1
Synonyms:SASP
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length343 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Homodimer Potential. UniProtKB Q09PK2

Subcellular location

Membrane; Single-pass membrane protein Potential.

Tissue specificity

Expressed primarily in the granular layer of the epidermis and inner root sheath of hair follicles. In psoriatic skin, expressed throughout the stratum corneum. In ulcerated skin, expressed in the stratum granulosum of intact epidermis but almost absent from ulcerated regions. Expressed in differentiated areas of squamous cell carcinomas but not in undifferentiated tumors. Ref.1 Ref.5

Post-translational modification

Undergoes autocleavage which is necessary for activation of the protein. Ref.1

Sequence similarities

Contains 1 peptidase A2 domain.

Sequence caution

The sequence BAB71587.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 190190 Ref.1
PRO_0000271170
Chain191 – 326136Retroviral-like aspartic protease 1 Ref.1
PRO_0000271171
Propeptide327 – 34317 Ref.1
PRO_0000271172

Regions

Topological domain1 – 5555Cytoplasmic Potential
Transmembrane56 – 7621Helical; Potential
Topological domain77 – 343267Extracellular Potential
Domain207 – 28882Peptidase A2

Sites

Active site2121 By similarity

Amino acid modifications

Glycosylation2761N-linked (GlcNAc...) Potential

Natural variations

Natural variant491T → A. Ref.2 Ref.4
Corresponds to variant rs3796097 [ dbSNP | Ensembl ].
VAR_051508

Experimental info

Mutagenesis2121D → A or E: Abolishes production of active form of enzyme. Ref.1
Sequence conflict311Q → R in BAB71587. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q53RT3 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 439BAC407F5EBCCB

FASTA34336,991
        10         20         30         40         50         60 
MGSPGASLGI KKALQSEQAT ALPASAPAVS QPTAPAPSCL PKAGQVIPTL LREAPFSSVI 

        70         80         90        100        110        120 
APTLLCGFLF LAWVAAEVPE ESSRMAGSGA RSEEGRRQHA FVPEPFDGAN VVPNLWLHSF 

       130        140        150        160        170        180 
EVINDLNHWD HITKLRFLKE SLRGEALGVY NRLSPQDQGD YGTVKEALLK AFGVPGAAPS 

       190        200        210        220        230        240 
HLPKEIVFAN SMGKGYYLKG KIGKVPVRFL VDSGAQVSVV HPNLWEEVTD GDLDTLQPFE 

       250        260        270        280        290        300 
NVVKVANGAE MKILGVWDTA VSLGKLKLKA QFLVANASAE EAIIGTDVLQ DHNAILDFEH 

       310        320        330        340 
RTCTLKGKKF RLLPVGGSLE DEFDLELIEE DPSSEEGRQE LSH 

« Hide

References

« Hide 'large scale' references
[1]"Identification and characterization of a novel retroviral-like aspartic protease specifically expressed in human epidermis."
Bernard D., Mehul B., Thomas-Collignon A., Delattre C., Donovan M., Schmidt R.
J. Invest. Dermatol. 125:278-287(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, TISSUE SPECIFICITY, AUTOCATALYTIC CLEAVAGE, MUTAGENESIS OF ASP-212.
Tissue: Epidermis.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-49.
Tissue: Cerebellum and Teratocarcinoma.
[3]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-49.
Tissue: Skin.
[5]"A novel aspartic proteinase-like gene expressed in stratified epithelia and squamous cell carcinoma of the skin."
Rhiemeier V., Breitenbach U., Richter K.H., Gebhardt C., Vogt I., Hartenstein B., Fuerstenberger G., Mauch C., Hess J., Angel P.
Am. J. Pathol. 168:1354-1364(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK055994 mRNA. Translation: BAB71067.1.
AK057813 mRNA. Translation: BAB71587.1. Different initiation.
AC079811 Genomic DNA. Translation: AAY24017.1.
BC031997 mRNA. Translation: AAH31997.2.
BC094002 mRNA. Translation: AAH94002.1.
BC094000 mRNA. Translation: AAH94000.1.
RefSeqNP_690005.2. NM_152792.2.
UniGeneHs.516253.

3D structure databases

ProteinModelPortalQ53RT3.
SMRQ53RT3. Positions 197-305.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid127385. 2 interactions.
IntActQ53RT3. 1 interaction.
STRING9606.ENSP00000315383.

Protein family/group databases

MEROPSA33.001.

PTM databases

PhosphoSiteQ53RT3.

Polymorphism databases

DMDM74726595.

Proteomic databases

PaxDbQ53RT3.
PRIDEQ53RT3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000320256; ENSP00000315383; ENSG00000244617.
GeneID151516.
KEGGhsa:151516.
UCSCuc002sfz.4. human.

Organism-specific databases

CTD151516.
GeneCardsGC02M070187.
H-InvDBHIX0002133.
HGNCHGNC:26321. ASPRV1.
HPAHPA034809.
HPA034810.
MIM611765. gene.
neXtProtNX_Q53RT3.
PharmGKBPA162376919.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG45468.
HOGENOMHOG000034019.
HOVERGENHBG080861.
InParanoidQ53RT3.
OMAKVANGAE.
OrthoDBEOG7SR4N6.
PhylomeDBQ53RT3.
TreeFamTF337956.

Gene expression databases

BgeeQ53RT3.
CleanExHS_ASPRV1.
GenevestigatorQ53RT3.

Family and domain databases

InterProIPR001969. Aspartic_peptidase_AS.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
SUPFAMSSF50630. SSF50630. 1 hit.
PROSITEPS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi151516.
NextBio86714.
PROQ53RT3.
SOURCESearch...

Entry information

Entry nameAPRV1_HUMAN
AccessionPrimary (citable) accession number: Q53RT3
Secondary accession number(s): Q8N5P2, Q96LT3, Q96N43
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: May 24, 2005
Last modified: February 19, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM