Reviewed, UniProtKB/Swiss-Prot Q53RB0 (LOX4_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable lipoxygenase 4
    EC=1.13.11.12

Gene names

Ordered Locus Names:

Os03g0700700, LOC_Os03g49380

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	877 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. Catalyzes the hydroperoxidation of lipids, containing a cis,cis-1,4-pentadiene structure By similarity.
Catalytic activity	Linoleate + O2 = (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Cofactor	Binds 1 iron ion per subunit. Iron is tightly bound By similarity.
Pathway	Lipid metabolism; oxylipin biosynthesis.
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.
Sequence caution	The sequence AK072689 differs from that shown. Reason: Frameshift at position 686.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis Oxylipin biosynthesis
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxylipin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW lipoxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 877	877	Probable lipoxygenase 4		PRO_0000220710
Regions
	Domain	38 – 165	128	PLAT
	Domain	168 – 877	710	Lipoxygenase
Sites
	Metal binding	528	1	Iron; catalytic By similarity
	Metal binding	533	1	Iron; catalytic By similarity
	Metal binding	719	1	Iron; catalytic By similarity
	Metal binding	723	1	Iron; catalytic By similarity
	Metal binding	877	1	Iron; via carboxylate; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q53RB0-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 9B4EBB61CDCD9B7C	FASTA	877	98,698
	10 20 30 40 50 60 MQVQGFFDRL TGRNKEAWKE GRIRGTAVLV KKDVLGLGDF HASLLDGVHN ILGHKEGVAF 70 80 90 100 110 120 RLVSATARDP SNGGRGKLGK PAHLEELVVT MKSTAAGESV FRVAFEWDES QGIPGAVVVT 130 140 150 160 170 180 NSNRSEFFLK TLTLDGVPGK GTVVFVANSW IYPADNYQYE RVFFANDTYL PSKMPAPLIP 190 200 210 220 230 240 YRQEELNILR GDGKIGPYKE HDRIYRYDYY NDLGQPDKGS KLVRPVLGGS QELPYPRRGR 250 260 270 280 290 300 TGRAPTKTDP NTESRLPLLD LNIYVPRDER FGHLKMSDFL GYSLKAIVEG VLPIIRTYVD 310 320 330 340 350 360 TTPKEFDSFQ DIMELYEGGL KVANASALAE IKKRVPFELI KSLLPVAGDQ VLKLPLPHVI 370 380 390 400 410 420 KEDKFAWRTD EEFAREMLAG VNPVMIKRLT NFPAKSTLDP NVYGDHTSKI TEAHIKHNME 430 440 450 460 470 480 GLTVQNALKG NRLFILDHHD HFMPFLDKIN KLDGNFIYAS RTILLLKDDG TLKPLAIELS 490 500 510 520 530 540 LPHPDGQQHG AVSKVYTPAN TGVESQIWQL AKAYASVNDS AWHQLISHWL NTHAVIEPFV 550 560 570 580 590 600 IATNRQLSVV HPVHKLLSPH YRDTMNINAL ARQTLINADG IFEKTVFPGK YALEMSSVVY 610 620 630 640 650 660 KNWKFTEQAL PVDLVKRGVA VPDPTSPYNV RLLIKDYPYA VDGLVIWWAI ERWVGEYLAI 670 680 690 700 710 720 YYPNDGVLRG DEELQAWWKE VREVGHGDLK DQDWWPKMDT VQELTRACTI IIWIASALHA 730 740 750 760 770 780 AVNFGQYPYA GFLPNRPTVS RRPMPEPGTE EYAKLERGGD EADLVFIHTI TSQFQTILGI 790 800 810 820 830 840 SLIEILSKHS SDEVYLGQRD TPEWTSDAKA LDAFKRFGSR LVDIENRIKD MNGNSALKNR 850 860 870 NGPVKMPYML LYPNTSDVTK EKGQGLTAMG IPNSISI

References

[1]

"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

[2]

"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC093017 Genomic DNA. Translation: AAX95641.1. AK072689 mRNA. No translation available.
3D structure databases
SMR	Q53RB0. Positions 21-877.
ModBase	Search...
Organism-specific databases
Gramene	Q53RB0.
Family and domain databases
InterPro	IPR008976. Lipase_LipOase. IPR000907. LipOase. IPR013819. LipOase_C. IPR020834. LipOase_CS. IPR020833. LipOase_Fe_BS. IPR001024. LipOase_LH2. IPR001246. LipOase_pln. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
PANTHER	PTHR11771. LipOase. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00468. PLTLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LOX4_ORYSJ

Accession

Primary (citable) accession number: Q53RB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	May 24, 2005
Last modified:	January 19, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)