Q53NI2 (NADK2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable NAD kinase 2, chloroplastic
EC=2.7.1.23

Gene names

Ordered Locus Names:

Os11g0191400, LOC_Os11g08670

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	981 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in chlorophyll synthesis and chloroplast protection against oxidative damage By similarity.
Catalytic activity	ATP + NAD+ = ADP + NADP+.
Subcellular location	Plastid › chloroplast By similarity.
Sequence similarities	Belongs to the NAD kinase family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Calmodulin-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	NAD metabolic process Inferred from electronic annotation. Source: InterPro NADP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 981		Probable NAD kinase 2, chloroplastic		PRO_0000233706
Regions
	Region	319 – 364	46	Calmodulin-binding By similarity
Experimental info
	Sequence conflict	610	1	K → R Ref.2
	Sequence conflict	809	1	L → V Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q53NI2 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 18DECA59F458B6B6	FASTA	981	108,613
	10 20 30 40 50 60 MLAVCARHGP AKLPPPPPPL AGERAAAWVV GRWWWRPAAA GRRGVVAARA SFFSSRIGLD 70 80 90 100 110 120 SQNYHTRDLS QLLWVGPVPG DIAEIEAYCR IFRAAEQLHT AVMSALCDPE TGECPVRYDV 130 140 150 160 170 180 QTEDLPVLED KVAAVLGCML ALLNRGRKEV LSGRSGVASA FQGSEDSTMD KIPPLALFRG 190 200 210 220 230 240 DLKRCCESMQ VALASYLVPS EARGLDIWRK LQRLKNACYD AGFPRADGHP CPTLFANWFP 250 260 270 280 290 300 VYFSTVPDDS LSDELEVAFW RGGQVSEEGL EWLLLKGFKT IVDLREEDVK DDLYLSAIHE 310 320 330 340 350 360 AVSLGKIEVV NLPVEIGTAP SAEQVQRFAE IVSDSAKKPI YLHSQEGISR TSAMVSRWKQ 370 380 390 400 410 420 YVTRAERLAT QNRSLNGNGK HVRNDQTEQL TNSPGFSSEG SENGTPLESD RTMEGETCDI 430 440 450 460 470 480 DIETARHNLE ITNSLPSEQS TEQGELHGTR TELQSNFRLE SNPLKAQFPS CDVFSKKGMT 490 500 510 520 530 540 DFFRSKKVYP KSVLNPRRRS NSLLVSRRKQ SLSAEQNGAI DYEAAEFKVL KSSNGASFDN 550 560 570 580 590 600 DYILSVASGI TNGKPSNNGA STSVEDREME TSVVTVDPRT SDTSNSNGNA PLGSQKSAER 610 620 630 640 650 660 NGSLYVEREK SDHVDGNMCA SATGVVRLQS RRKAEMFLVR TDGFSCTREK VTESSLAFTH 670 680 690 700 710 720 PSTQQQMLMW KSPPKTVLLL KKLGDELMEE AKEVASFLHH QEKMNVLVEP DVHDIFARIP 730 740 750 760 770 780 GYGFVQTFYT QDTSDLHERV DFVACLGGDG VILHASNLFR TSVPPVVSFN LGSLGFLTSH 790 800 810 820 830 840 NFEGFRQDLR AVIHGNNTLG VYITLRMRLR CEIFRNGKAM PGKVFDVLNE VVVDRGSNPY 850 860 870 880 890 900 LSKIECYEHN HLITKVQGDG VIVATPTGST AYSTAAGGSM VHPNVPCMLF TPICPHSLSF 910 920 930 940 950 960 RPVILPDSAR LELKIPDDAR SNAWVSFDGK RRQQLSRGDS VQISMSQHPL PTVNKSDQTG 970 980 DWFRSLIRCL NWNERLDQKA L

References

[1]	"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC134046 Genomic DNA. Translation: AAX95220.1. DP000010 Genomic DNA. Translation: ABA91849.1. AK065215 mRNA. No translation available.
RefSeq	NP_001067415.1. NM_001073947.1.
UniGene	Os.87857.
3D structure databases
ProteinModelPortal	Q53NI2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os11g08670.1; LOC_Os11g08670.1; LOC_Os11g08670.
GeneID	4349979.
KEGG	osa:4349979.
Organism-specific databases
Gramene	Q53NI2.
Phylogenomic databases
eggNOG	COG0061.
HOGENOM	HOG000029602.
OMA	HERVDFV.
ProtClustDB	PLN02727.
Gene expression databases
ArrayExpress	Q53NI2.
Family and domain databases
Gene3D	2.60.200.30. 1 hit. 3.40.50.10330. 1 hit.
InterPro	IPR017438. ATP-NAD_kinase_dom_1. IPR016064. ATP-NAD_kinase_PpnK-typ. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. PolyP/ATP_NADK_prd. [Graphical view]
PANTHER	PTHR20275. PTHR20275. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
SUPFAM	SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADK2_ORYSJ

Accession

Primary (citable) accession number: Q53NI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	May 24, 2005
Last modified:	April 3, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program