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Q53N72 (MPK15_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 15

Short name=MAP kinase 15
EC=2.7.11.24
Gene names
Name:MPK15
Ordered Locus Names:Os11g0271100, LOC_Os11g17080
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length498 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by threonine and tyrosine phosphorylation By similarity.

Induction

By jasmonic acid (JA) and infection with rice blast fungus (M.grisea). Ref.3

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

vacuole

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

MAP kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 498498Mitogen-activated protein kinase 15
PRO_0000239758

Regions

Domain13 – 304292Protein kinase
Nucleotide binding19 – 279ATP By similarity
Motif175 – 1773TXY

Sites

Active site1391Proton acceptor By similarity
Binding site421ATP By similarity

Amino acid modifications

Modified residue1751Phosphothreonine By similarity
Modified residue1771Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q53N72 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: EBC9F67E62DF299D

FASTA49856,967
        10         20         30         40         50         60 
MDFFTEYGEG NRYKIEEVIG KGSYGVVCSA LDTHTGEKVA IKKINDIFEH VSDATRILRE 

        70         80         90        100        110        120 
IKLLRLLRHP DIVEIKHILL PPSRREFKDI YVVFELMESD LHQVIKANDD LTPEHYQFFL 

       130        140        150        160        170        180 
YQLLRGLKYI HTANVFHRDL KPKNILANAD CKLKICDFGL ARVAFSDTPT AIFWTDYVAT 

       190        200        210        220        230        240 
RWYRAPELCG SFFSKYTPAI DIWSIGCIFA ELLTGKPLFP GKNVVHQLDI ITDLLGTPST 

       250        260        270        280        290        300 
EAISRIRNEK ARRYLSSMRR KKPIPFTQKF PNADPLALRL LERMLSFEPK DRPNAEEALA 

       310        320        330        340        350        360 
DPYFRNIANV DREPSAQPVT KLEFEFERRR ITKEDIRELI YRDILEYHPN MLREYLEGTE 

       370        380        390        400        410        420 
SAGFMYPSAV DHFKKQFAYL EEHYAKGSTA APPERQHNSL PRPSVLYSDD RPQNTANIAE 

       430        440        450        460        470        480 
DLSKCVLGDN TQKMHQGSAS VCANRVPQGG AARPGKVVGS ALRYGNCSTS TAEQYEHRRT 

       490 
DRNPALATNT VSPRGSYP 

« Hide

References

« Hide 'large scale' references
[1]"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications."
The rice chromosomes 11 and 12 sequencing consortia
BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.
[3]"Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
Reyna N.S., Yang Y.
Mol. Plant Microbe Interact. 19:530-540(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC135497 Genomic DNA. Translation: AAX94956.1.
DP000010 Genomic DNA. Translation: ABA92667.1.
AK068981 mRNA. No translation available.

3D structure databases

ProteinModelPortalQ53N72.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ53N72.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS11T0271100-01; OS11T0271100-01; OS11G0271100.

Organism-specific databases

GrameneQ53N72.

Phylogenomic databases

eggNOGCOG0515.
OMAMRKKKPI.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPK15_ORYSJ
AccessionPrimary (citable) accession number: Q53N72
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: May 24, 2005
Last modified: April 16, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations