Reviewed, UniProtKB/Swiss-Prot Q53LU4 (LAC18_ORYSJ)

Names and origin

Protein names

Recommended name:
    Laccase-18
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 18
    Urishiol oxidase 18
    Diphenol oxidase 18

Gene names

Name:	LAC18
Ordered Locus Names:	Os11g0264000, LOC_Os11g16260
ORF Names:	OsJ_032226

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products By similarity.
Catalytic activity	4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Apoplast Secreted
Domain	Repeat Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 595	566	Laccase-18		PRO_0000291903
Regions
	Domain	37 – 153	117	Plastocyanin-like 1
	Domain	162 – 316	155	Plastocyanin-like 2
	Domain	429 – 571	143	Plastocyanin-like 3
Sites
	Metal binding	87	1	Copper 1 By similarity
	Metal binding	89	1	Copper 2 By similarity
	Metal binding	132	1	Copper 2 By similarity
	Metal binding	134	1	Copper 3 By similarity
	Metal binding	488	1	Copper 4 Potential
	Metal binding	491	1	Copper 1 By similarity
	Metal binding	493	1	Copper 3 By similarity
	Metal binding	550	1	Copper 3 By similarity
	Metal binding	551	1	Copper 4 Potential
	Metal binding	552	1	Copper 2 By similarity
	Metal binding	556	1	Copper 4 Potential
	Metal binding	561	1	Copper 4 Potential
Amino acid modifications
	Glycosylation	42	1	N-linked (GlcNAc...) Potential
	Glycosylation	48	1	N-linked (GlcNAc...) Potential
	Glycosylation	121	1	N-linked (GlcNAc...) Potential
	Glycosylation	206	1	N-linked (GlcNAc...) Potential
	Glycosylation	345	1	N-linked (GlcNAc...) Potential
	Glycosylation	382	1	N-linked (GlcNAc...) Potential
	Glycosylation	402	1	N-linked (GlcNAc...) Potential
	Glycosylation	409	1	N-linked (GlcNAc...) Potential
	Glycosylation	439	1	N-linked (GlcNAc...) Potential
	Glycosylation	470	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q53LU4-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: ED20952A25B72CAF	FASTA	595	66,189
	10 20 30 40 50 60 MEKLSTAASL FCVVVAATAL AMAVVGGEAA VVEQTFMVHE MNVTHLCNTT KIYVVNGRFP 70 80 90 100 110 120 GPTVDVTEGD TVVVHVINRL PHGLTIHWHG VRQMRSCWAD GAGYVTECPI HPGGEKTYRF 130 140 150 160 170 180 NVTGQVGTLW WHAHVTCLRA TINGAFIIRP RNGKYPFLTP AKDVPIIIGE WWELDLIELD 190 200 210 220 230 240 RRMLDGNFDD NPLSATINGK LGDLSNCSST VEESFVLDVK RGESYLLRVI NTALFSEYYF 250 260 270 280 290 300 KVAGHTFTVV GADGNYLTPY KTDMVTVAPG EAIDVLMFTD APPAYYHMVA LANQPPPPDL 310 320 330 340 350 360 QIPQLTSRGL IRYAGAAMDS NNLPMPMPVM PDQHNTMPSY YFRRNLTGLA LPEQQQRHRV 370 380 390 400 410 420 PAHVDERLLI TLGLGSICRG GNTTTCKRGR SPETVVVATM NNVSFHHTNA TALLEHYYDG 430 440 450 460 470 480 RPEGVYTEDF PVRPPRPFNY TDRELIPAGP LEAALEPTAK AMRLRRFRYN ASVEIVFQST 490 500 510 520 530 540 TLLQSDSNPM HLHGYDVFVL AQGLGNFDPK RDVEKFNYHN PQLRNTVQVP RGGWAAVRFL 550 560 570 580 590 ADNPGMWYLH CHFEFHIIMG MATAFIVEDG PTPETSLPPP PPEFKRCGTN GLSQP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed: 16188032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC138197 Genomic DNA. Translation: AAX96096.1. DP000010 Genomic DNA. Translation: ABA92481.1. AP008217 Genomic DNA. Translation: BAF28013.1. CM000148 Genomic DNA. Translation: EAZ18017.1.
RefSeq	NP_001067650.1.
UniGene	Os.86101
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4350236.
KEGG	osa:4350236.
Organism-specific databases
Gramene	Q53LU4.
Family and domain databases
InterPro	IPR001117. Cu-oxidase. IPR011706. Cu-oxidase_2. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 3 hits.
Pfam	PF00394. Cu-oxidase. 1 hit. PF07731. Cu-oxidase_2. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view]
PROSITE	PS00079. MULTICOPPER_OXIDASE1. 1 hit. PS00080. MULTICOPPER_OXIDASE2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC18_ORYSJ

Accession

Primary (citable) accession number: Q53LU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	May 24, 2005
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)