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Protein

Probable sucrose-phosphate synthase 5

Gene

SPS5

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role in photosynthetic sucrose synthesis by catalyzing the rate-limiting step of sucrose biosynthesis from UDP-glucose and fructose- 6-phosphate. Involved in the regulation of carbon partitioning in the leaves of plants. May regulate the synthesis of sucrose and therefore play a major role as a limiting factor in the export of photoassimilates out of the leaf. Plays a role for sucrose availability that is essential for plant growth and fiber elongation (By similarity).By similarity

Catalytic activityi

UDP-glucose + D-fructose 6-phosphate = UDP + sucrose 6(F)-phosphate.

Enzyme regulationi

Activity is regulated by phosphorylation and moderated by concentration of metabolites and light.By similarity

Pathwayi: sucrose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sucrose from D-fructose 6-phosphate and UDP-alpha-D-glucose.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable sucrose-phosphate synthase 5 (SPS5), Probable sucrose-phosphate synthase 4 (SPS4), Probable sucrose-phosphate synthase 3 (SPS3), Probable sucrose-phosphate synthase 1 (SPS1), Probable sucrose-phosphate synthase 2 (SPS2)
  2. Probable sucrose-phosphatase 3 (SPP3), Probable sucrose-phosphatase 1 (SPP1), Sucrose-phosphatase 2 (SPP2)
This subpathway is part of the pathway sucrose biosynthesis, which is itself part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sucrose from D-fructose 6-phosphate and UDP-alpha-D-glucose, the pathway sucrose biosynthesis and in Glycan biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.14. 4460.
UniPathwayiUPA00371; UER00545.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable sucrose-phosphate synthase 5 (EC:2.4.1.14)
Alternative name(s):
Sucrose phosphate synthase 5F
Short name:
OsSPS5F
UDP-glucose-fructose-phosphate glucosyltransferase
Gene namesi
Name:SPS5
Synonyms:SPS11
Ordered Locus Names:Os11g0236100, LOC_Os11g12810
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 11, cultivar: Nipponbare

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10141014Probable sucrose-phosphate synthase 5PRO_0000413644Add
BLAST

Proteomic databases

PaxDbiQ53JI9.
PRIDEiQ53JI9.

PTM databases

iPTMnetiQ53JI9.

Expressioni

Tissue specificityi

Expressed in germinating seeds.1 Publication

Developmental stagei

Expressed in source leaves and sink leaves.1 Publication

Inductioni

Circadian-regulated, with the highest expression 1 hour after the beginning of dark period (in 14 hours light/10 hours dark cycle).1 Publication

Gene expression databases

ExpressionAtlasiQ53JI9. baseline and differential.

Interactioni

Subunit structurei

Homodimer or homotetramer.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os11g12810.1.

Structurei

3D structure databases

ProteinModelPortaliQ53JI9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 1 family.Curated

Phylogenomic databases

eggNOGiKOG0853. Eukaryota.
COG0438. LUCA.
InParanoidiQ53JI9.
KOiK00696.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR006380. SPP_N.
IPR012819. SucrsPsyn_pln.
[Graphical view]
PfamiPF13579. Glyco_trans_4_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
PF05116. S6PP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02468. sucrsPsyn_pln. 1 hit.

Sequencei

Sequence statusi: Complete.

Q53JI9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHHYSMSCP DLEAIEQVEW EFSRQLSRRR LEQELGSREA AADLSELSEG
60 70 80 90 100
EKDGKPDTHP PPAAAAAEAA ADDGGGGDHQ QQQQQPPPHQ LSRFARINSD
110 120 130 140 150
PRIVSDEEEE VTTDRNLYIV LISIHGLVRG ENMELGRDSD TGGQVKYVVE
160 170 180 190 200
LARALAATPG VHRVDLLTRQ ISCPDVDWTY GEPVEMLTVP AADADDEDGG
210 220 230 240 250
GGSSGGAYIV RLPCGPRDKY LPKESLWPHI PEFVDRALAH VTNVARALGE
260 270 280 290 300
QLSPPPPSDG AGAAAQAVWP YVIHGHYADA AEVAALLASA LNVPMVMTGH
310 320 330 340 350
SLGRNKLEQL LKLGRMPRAE IQGTYKIARR IEAEETGLDA ADMVVTSTKQ
360 370 380 390 400
EIEEQWGLYD GFDLKVERKL RVRRRRGVSC LGRYMPRMVV IPPGMDFSYV
410 420 430 440 450
DTQDLAADGA GGAGDAADLQ LLINPNKAKK PLPPIWSEVL RFFTNPHKPM
460 470 480 490 500
ILALSRPDPK KNVTTLLKAY GESRHLRELA NLTLILGNRD DIEEMSGGAA
510 520 530 540 550
TVLTAVLKLI DRYDLYGQVA YPKHHKQTDV PHIYRLAAKT KGVFINPALV
560 570 580 590 600
EPFGLTIIEA AAYGLPVVAT KNGGPVDILK VLSNGLLVDP HDAAAITAAL
610 620 630 640 650
LSLLADKSRW SECRRSGLRN IHRFSWPHHC RLYLSHVAAS CDHPAPHQLL
660 670 680 690 700
RVPPSPSSSS AAAAAAGGGG AAASSEPLSD SLRDLSLRIS VDAASPDLSA
710 720 730 740 750
GDSAAAILDA LRRRRSTDRP AASSAARAIG FAPGRRQSLL VVAIDCYGDD
760 770 780 790 800
GKPNVEQLKK VVELAMSAGD GDDAGGRGYV LSTGMTIPEA VDALRACGAD
810 820 830 840 850
PAGFDALICS SGAEICYPWK GEQLAADEEY AGHVAFRWPG DHVRSAVPRL
860 870 880 890 900
GKADGAQEAD LAVDAAACSV HCHAYAAKDA SKVKKVDWIR QALRMRGFRC
910 920 930 940 950
NLVYTRACTR LNVVPLSASR PRALRYLSIQ WGIDLSKVAV LVGEKGDTDR
960 970 980 990 1000
ERLLPGLHRT VILPGMVAAG SEELLRDEDG FTTEDVVAMD SPNIVTLADG
1010
QDIAAAAADL LKAI
Length:1,014
Mass (Da):108,985
Last modified:May 24, 2005 - v1
Checksum:i6AF4C8059E23B842
GO

Sequence cautioni

The sequence BAF27932.2 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC135258 Genomic DNA. Translation: AAX96649.1.
AC147811 Genomic DNA. Translation: AAX95196.1.
DP000010 Genomic DNA. Translation: ABA92286.1.
AP008217 Genomic DNA. Translation: BAF27932.2. Sequence problems.
AP014967 Genomic DNA. No translation available.
RefSeqiXP_015617675.1. XM_015762189.1.
UniGeneiOs.60812.

Genome annotation databases

GeneIDi4350151.
KEGGiosa:4350151.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC135258 Genomic DNA. Translation: AAX96649.1.
AC147811 Genomic DNA. Translation: AAX95196.1.
DP000010 Genomic DNA. Translation: ABA92286.1.
AP008217 Genomic DNA. Translation: BAF27932.2. Sequence problems.
AP014967 Genomic DNA. No translation available.
RefSeqiXP_015617675.1. XM_015762189.1.
UniGeneiOs.60812.

3D structure databases

ProteinModelPortaliQ53JI9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os11g12810.1.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

PTM databases

iPTMnetiQ53JI9.

Proteomic databases

PaxDbiQ53JI9.
PRIDEiQ53JI9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4350151.
KEGGiosa:4350151.

Phylogenomic databases

eggNOGiKOG0853. Eukaryota.
COG0438. LUCA.
InParanoidiQ53JI9.
KOiK00696.

Enzyme and pathway databases

UniPathwayiUPA00371; UER00545.
BRENDAi2.4.1.14. 4460.

Gene expression databases

ExpressionAtlasiQ53JI9. baseline and differential.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR006380. SPP_N.
IPR012819. SucrsPsyn_pln.
[Graphical view]
PfamiPF13579. Glyco_trans_4_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
PF05116. S6PP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02468. sucrsPsyn_pln. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications."
    The rice chromosomes 11 and 12 sequencing consortia
    BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  5. "Phylogenetic and expression analysis of sucrose phosphate synthase isozymes in plants."
    Lutfiyya L.L., Xu N., D'Ordine R.L., Morrell J.A., Miller P.W., Duff S.M.
    J. Plant Physiol. 164:923-933(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  6. "Tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family in rice."
    Okamura M., Aoki N., Hirose T., Yonekura M., Ohto C., Ohsugi R.
    Plant Sci. 181:159-166(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.

Entry informationi

Entry nameiSPSA5_ORYSJ
AccessioniPrimary (citable) accession number: Q53JI9
Secondary accession number(s): Q0ITN3, Q53NB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: May 24, 2005
Last modified: May 11, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.