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Protein

Plectasin

Gene

DEF

Organism
Pseudoplectania nigrella (Ebony cup)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a potent antibiotic activity against several species of Gram-positive bacteria. Especially active against numerous clinical isolates of S.pneumoniae, including all 90 different serotypes and isolates resistant to clinically used antibiotics. Shows considerable selectivity for bacteria over mammalian cells in vitro.1 Publication

Miscellaneous

In mice infected by S.pneumoniae, it has an efficacy comparable to vancomycin in the peritoneal model and to penicillin in the pneumonia model.

GO - Biological processi

Keywordsi

Molecular functionAntibiotic, Antimicrobial, Defensin

Protein family/group databases

TCDBi1.C.47.2.1. the insect/fungal defensin (insect/fungal defensin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Plectasin
Gene namesi
Name:DEF
OrganismiPseudoplectania nigrella (Ebony cup)
Taxonomic identifieri96584 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaPezizomycetesPezizalesSarcosomataceaePseudoplectania

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Pharmaceutical usei

Plectasin could be used as a possible antibiotic, especially against S.pneumoniae infections.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
PropeptideiPRO_000004275324 – 551 PublicationAdd BLAST32
ChainiPRO_000004275456 – 95PlectasinAdd BLAST40

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi59 ↔ 85PROSITE-ProRule annotation1 Publication
Disulfide bondi70 ↔ 92PROSITE-ProRule annotation1 Publication
Disulfide bondi74 ↔ 94PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Structurei

Secondary structure

195
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi62 – 64Combined sources3
Helixi67 – 76Combined sources10
Beta strandi82 – 86Combined sources5
Turni87 – 90Combined sources4
Beta strandi91 – 95Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZFUNMR-A56-95[»]
3E7RX-ray1.00L56-95[»]
3E7UX-ray1.35X56-95[»]
ProteinModelPortaliQ53I06.
SMRiQ53I06.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ53I06.

Family & Domainsi

Sequence similaritiesi

Belongs to the invertebrate defensin family. Type 2 subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiView protein in InterPro
IPR001542. Defensin_invertebrate/fungal.
IPR036574. Scorpion_toxin-like_sf.
PfamiView protein in Pfam
PF01097. Defensin_2. 1 hit.
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiView protein in PROSITE
PS51378. INVERT_DEFENSINS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q53I06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFTTILSIG ITVFGLLNTG AFAAPQPVPE AYAVSDPEAH PDDFAGMDAN
60 70 80 90
QLQKRGFGCN GPWDEDDMQC HNHCKSIKGY KGGYCAKGGF VCKCY
Length:95
Mass (Da):10,254
Last modified:May 24, 2005 - v1
Checksum:i882903AB610AB018
GO

Mass spectrometryi

Molecular mass is 4398.8 Da from positions 56 - 95. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ964941 mRNA. Translation: CAI83768.1.

Similar proteinsi

Entry informationi

Entry nameiPLECT_PSENR
AccessioniPrimary (citable) accession number: Q53I06
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: May 24, 2005
Last modified: February 28, 2018
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Pharmaceutical