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Protein

Plectasin

Gene

DEF

Organism
Pseudoplectania nigrella (Ebony cup)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has a potent antibiotic activity against several species of Gram-positive bacteria. Especially active against numerous clinical isolates of S.pneumoniae, including all 90 different serotypes and isolates resistant to clinically used antibiotics. Shows considerable selectivity for bacteria over mammalian cells in vitro.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Defensin

Protein family/group databases

TCDBi1.C.47.2.1. the insect/fungal defensin (insect/fungal defensin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Plectasin
Gene namesi
Name:DEF
OrganismiPseudoplectania nigrella (Ebony cup)
Taxonomic identifieri96584 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaPezizomycetesPezizalesSarcosomataceaePseudoplectania

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Pharmaceutical usei

Plectasin could be used as a possible antibiotic, especially against S.pneumoniae infections.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Propeptidei24 – 55321 PublicationPRO_0000042753Add
BLAST
Chaini56 – 9540PlectasinPRO_0000042754Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi59 ↔ 85PROSITE-ProRule annotation1 Publication
Disulfide bondi70 ↔ 92PROSITE-ProRule annotation1 Publication
Disulfide bondi74 ↔ 94PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Structurei

Secondary structure

1
95
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi62 – 643Combined sources
Helixi67 – 7610Combined sources
Beta strandi82 – 865Combined sources
Turni87 – 904Combined sources
Beta strandi91 – 955Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZFUNMR-A56-95[»]
3E7RX-ray1.00L56-95[»]
3E7UX-ray1.35X56-95[»]
ProteinModelPortaliQ53I06.
SMRiQ53I06. Positions 56-95.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ53I06.

Family & Domainsi

Sequence similaritiesi

Belongs to the invertebrate defensin family. Type 2 subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR001542. Defensin_invertebrate/fungal.
IPR003614. Scorpion_toxin-like.
[Graphical view]
PfamiPF01097. Defensin_2. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS51378. INVERT_DEFENSINS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q53I06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFTTILSIG ITVFGLLNTG AFAAPQPVPE AYAVSDPEAH PDDFAGMDAN
60 70 80 90
QLQKRGFGCN GPWDEDDMQC HNHCKSIKGY KGGYCAKGGF VCKCY
Length:95
Mass (Da):10,254
Last modified:May 24, 2005 - v1
Checksum:i882903AB610AB018
GO

Mass spectrometryi

Molecular mass is 4398.8 Da from positions 56 - 95. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ964941 mRNA. Translation: CAI83768.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ964941 mRNA. Translation: CAI83768.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZFUNMR-A56-95[»]
3E7RX-ray1.00L56-95[»]
3E7UX-ray1.35X56-95[»]
ProteinModelPortaliQ53I06.
SMRiQ53I06. Positions 56-95.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.C.47.2.1. the insect/fungal defensin (insect/fungal defensin) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ53I06.

Family and domain databases

InterProiIPR001542. Defensin_invertebrate/fungal.
IPR003614. Scorpion_toxin-like.
[Graphical view]
PfamiPF01097. Defensin_2. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS51378. INVERT_DEFENSINS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLECT_PSENR
AccessioniPrimary (citable) accession number: Q53I06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: May 24, 2005
Last modified: December 9, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In mice infected by S.pneumoniae, it has an efficacy comparable to vancomycin in the peritoneal model and to penicillin in the pneumonia model.

Keywords - Technical termi

3D-structure, Direct protein sequencing, Pharmaceutical

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.