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Protein

Pyrroline-5-carboxylate reductase 3

Gene

PYCRL

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.

Pathway:iL-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-proline from L-glutamate 5-semialdehyde.
Proteins known to be involved in this subpathway in this organism are:
  1. Pyrroline-5-carboxylate reductase, Pyrroline-5-carboxylate reductase (P5CR2), Pyrroline-5-carboxylate reductase (PYCRL), Pyrroline-5-carboxylate reductase 1, mitochondrial (PYCR1), Pyrroline-5-carboxylate reductase 2 (PYCR2), Pyrroline-5-carboxylate reductase 3 (PYCRL), Pyrroline-5-carboxylate reductase (PYCR1), Pyrroline-5-carboxylate reductase (PYCR2), Pyrroline-5-carboxylate reductase (PYCRL), Pyrroline-5-carboxylate reductase (PYCR2), Pyrroline-5-carboxylate reductase (PYCR1), Pyrroline-5-carboxylate reductase (PYCR1), Pyrroline-5-carboxylate reductase
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-proline from L-glutamate 5-semialdehyde, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00098; UER00361.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrroline-5-carboxylate reductase 3 (EC:1.5.1.2)
Short name:
P5C reductase 3
Short name:
P5CR 3
Alternative name(s):
Pyrroline-5-carboxylate reductase-like protein
Gene namesi
Name:PYCRL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:25846. PYCRL.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134889043.

Chemistry

DrugBankiDB00172. L-Proline.

Polymorphism and mutation databases

BioMutaiPYCRL.
DMDMi172046829.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 274273Pyrroline-5-carboxylate reductase 3PRO_0000324561Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ53H96.
PeptideAtlasiQ53H96.
PRIDEiQ53H96.

PTM databases

PhosphoSiteiQ53H96.

Expressioni

Gene expression databases

BgeeiQ53H96.
CleanExiHS_PYCRL.
ExpressionAtlasiQ53H96. baseline and differential.
GenevisibleiQ53H96. HS.

Interactioni

Subunit structurei

Homodecamer; composed of 5 homodimers.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
SDCBPO005603EBI-2959680,EBI-727004

Protein-protein interaction databases

BioGridi122418. 21 interactions.
IntActiQ53H96. 5 interactions.
STRINGi9606.ENSP00000220966.

Structurei

3D structure databases

ProteinModelPortaliQ53H96.
SMRiQ53H96. Positions 11-271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0345.
GeneTreeiENSGT00390000007443.
HOGENOMiHOG000230247.
HOVERGENiHBG053399.
InParanoidiQ53H96.
KOiK00286.
OrthoDBiEOG7N0C5N.

Family and domain databases

Gene3Di1.10.3730.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01925. P5C_reductase.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR029036. P5CR_dimer.
IPR028939. ProC_N.
IPR000304. Pyrroline-COOH_reductase.
[Graphical view]
PANTHERiPTHR11645. PTHR11645. 1 hit.
PfamiPF03807. F420_oxidored. 1 hit.
PF14748. P5CR_dimer. 1 hit.
[Graphical view]
PIRSFiPIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR00112. proC. 1 hit.
PROSITEiPS00521. P5CR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q53H96-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAEPSPRR VGFVGAGRMA GAIAQGLIRA GKVEAQHILA SAPTDRNLCH
60 70 80 90 100
FQALGCRTTH SNQEVLQSCL LVIFATKPHV LPAVLAEVAP VVTTEHILVS
110 120 130 140 150
VAAGVSLSTL EELLPPNTRV LRVLPNLPCV VQEGAIVMAR GRHVGSSETK
160 170 180 190 200
LLQHLLEACG RCEEVPEAYV DIHTGLSGSG VAFVCAFSEA LAEGAVKMGM
210 220 230 240 250
PSSLAHRIAA QTLLGTAKML LHEGQHPAQL RSDVCTPGGT TIYGLHALEQ
260 270
GGLRAATMSA VEAATCRAKE LSRK
Length:274
Mass (Da):28,663
Last modified:November 26, 2014 - v3
Checksum:i846FDEC603F3B548
GO
Isoform 2 (identifier: Q53H96-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     113-132: Missing.

Note: No experimental confirmation available.
Show »
Length:254
Mass (Da):26,440
Checksum:i0BF4B4DD70D009AF
GO

Sequence cautioni

The sequence BAD96405.1 differs from that shown.Chimeric cDNA.Curated
The sequence BAD96405.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti57 – 571R → Q.
Corresponds to variant rs11549789 [ dbSNP | Ensembl ].
VAR_039828
Natural varianti105 – 1051V → M.
Corresponds to variant rs2242089 [ dbSNP | Ensembl ].
VAR_039829
Natural varianti150 – 1501K → N.3 Publications
Corresponds to variant rs2242090 [ dbSNP | Ensembl ].
VAR_039830

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei113 – 13220Missing in isoform 2. 1 PublicationVSP_057219Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001500 mRNA. Translation: BAG50927.1.
AK023914 mRNA. Translation: BAB14721.1.
AK301225 mRNA. Translation: BAG62798.1.
AC067930 Genomic DNA. No translation available.
BC007993 mRNA. Translation: AAH07993.1.
AK222685 mRNA. Translation: BAD96405.1. Sequence problems.
AL833857 mRNA. Translation: CAD38716.1.
RefSeqiNP_075566.2. NM_023078.3.
UniGeneiHs.165186.

Genome annotation databases

EnsembliENST00000433751; ENSP00000404493; ENSG00000104524. [Q53H96-2]
ENST00000495276; ENSP00000480945; ENSG00000104524.
GeneIDi65263.
KEGGihsa:65263.
UCSCiuc011lkm.2. human.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001500 mRNA. Translation: BAG50927.1.
AK023914 mRNA. Translation: BAB14721.1.
AK301225 mRNA. Translation: BAG62798.1.
AC067930 Genomic DNA. No translation available.
BC007993 mRNA. Translation: AAH07993.1.
AK222685 mRNA. Translation: BAD96405.1. Sequence problems.
AL833857 mRNA. Translation: CAD38716.1.
RefSeqiNP_075566.2. NM_023078.3.
UniGeneiHs.165186.

3D structure databases

ProteinModelPortaliQ53H96.
SMRiQ53H96. Positions 11-271.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122418. 21 interactions.
IntActiQ53H96. 5 interactions.
STRINGi9606.ENSP00000220966.

Chemistry

DrugBankiDB00172. L-Proline.

PTM databases

PhosphoSiteiQ53H96.

Polymorphism and mutation databases

BioMutaiPYCRL.
DMDMi172046829.

Proteomic databases

PaxDbiQ53H96.
PeptideAtlasiQ53H96.
PRIDEiQ53H96.

Protocols and materials databases

DNASUi65263.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000433751; ENSP00000404493; ENSG00000104524. [Q53H96-2]
ENST00000495276; ENSP00000480945; ENSG00000104524.
GeneIDi65263.
KEGGihsa:65263.
UCSCiuc011lkm.2. human.

Organism-specific databases

CTDi65263.
GeneCardsiGC08M144686.
H-InvDBHIX0007842.
HGNCiHGNC:25846. PYCRL.
neXtProtiNX_Q53H96.
PharmGKBiPA134889043.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0345.
GeneTreeiENSGT00390000007443.
HOGENOMiHOG000230247.
HOVERGENiHBG053399.
InParanoidiQ53H96.
KOiK00286.
OrthoDBiEOG7N0C5N.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00361.

Miscellaneous databases

GenomeRNAii65263.
NextBioi35475549.
PROiQ53H96.

Gene expression databases

BgeeiQ53H96.
CleanExiHS_PYCRL.
ExpressionAtlasiQ53H96. baseline and differential.
GenevisibleiQ53H96. HS.

Family and domain databases

Gene3Di1.10.3730.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01925. P5C_reductase.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR029036. P5CR_dimer.
IPR028939. ProC_N.
IPR000304. Pyrroline-COOH_reductase.
[Graphical view]
PANTHERiPTHR11645. PTHR11645. 1 hit.
PfamiPF03807. F420_oxidored. 1 hit.
PF14748. P5CR_dimer. 1 hit.
[Graphical view]
PIRSFiPIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR00112. proC. 1 hit.
PROSITEiPS00521. P5CR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Spleen and Thyroid.
  2. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ASN-150.
    Tissue: Lung.
  4. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-264 (ISOFORM 1), VARIANT ASN-150.
    Tissue: Brain.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 31-274 (ISOFORM 1), VARIANT ASN-150.
    Tissue: Amygdala.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiP5CR3_HUMAN
AccessioniPrimary (citable) accession number: Q53H96
Secondary accession number(s): B3KMB5
, B4DVT6, H0Y6C3, Q8N3N9, Q96HX4, Q9H896
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: November 26, 2014
Last modified: July 22, 2015
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.