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Protein

Endoribonuclease LACTB2

Gene

LACTB2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Endoribonuclease; cleaves preferentially 3' to purine-pyrimidine dinucleotide motifs in single-stranded RNA. The cleavage product contains a free 3' -OH group. Has no activity with double-stranded RNA or DNA. Required for normal mitochondrial function and cell viability.1 Publication

Cofactori

Zn2+1 PublicationNote: Binds 2 Zn2+ ions per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi77Zinc 1; via tele nitrogenCombined sources1
Metal bindingi79Zinc 1; via pros nitrogenCombined sources1
Metal bindingi81Zinc 2Combined sources1
Metal bindingi82Zinc 2; via tele nitrogenCombined sources1
Metal bindingi118Zinc 1Combined sources1
Metal bindingi118Zinc 2Combined sources1
Metal bindingi145Zinc 1; via tele nitrogenCombined sources1
Metal bindingi164Zinc 1Combined sources1
Metal bindingi164Zinc 2Combined sources1
Metal bindingi199Zinc 2; via tele nitrogenCombined sources1

GO - Molecular functioni

  • endoribonuclease activity Source: UniProtKB
  • single-stranded RNA binding Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • RNA phosphodiester bond hydrolysis, endonucleolytic Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Endoribonuclease LACTB21 Publication (EC:3.1.27.-1 Publication)
Alternative name(s):
Beta-lactamase-like protein 2
Gene namesi
Name:LACTB2
ORF Names:CGI-83
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:18512. LACTB2.

Subcellular locationi

  • Mitochondrion matrix 1 Publication

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi77 – 82Missing : Loss of endoribonuclease activity. 1 Publication6
Mutagenesisi81D → A: Loss of endoribonuclease activity. 1 Publication1
Mutagenesisi110R → A: Mildly decreases endoribonuclease activity. No effect on RNA-binding. 1 Publication1
Mutagenesisi116N → A: Loss of endoribonuclease activity. No effect on RNA-binding. 1 Publication1
Mutagenesisi118E → A: Loss of endoribonuclease activity. No effect on RNA-binding. 1 Publication1
Mutagenesisi164D → A: Loss of endoribonuclease activity. 1 Publication1
Mutagenesisi216 – 217HR → AT: Loss of endoribonuclease activity. Strongly decreases RNA-binding. 1 Publication2
Mutagenesisi220R → T: Loss of endoribonuclease activity. Strongly decreases RNA-binding. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000147592.
PharmGKBiPA134952605.

Polymorphism and mutation databases

BioMutaiLACTB2.
DMDMi166223244.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003157431 – 288Endoribonuclease LACTB2Add BLAST288

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei102N6-acetyllysineBy similarity1
Modified residuei235PhosphoserineBy similarity1
Modified residuei273N6-succinyllysineBy similarity1
Modified residuei282N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ53H82.
MaxQBiQ53H82.
PaxDbiQ53H82.
PeptideAtlasiQ53H82.
PRIDEiQ53H82.

2D gel databases

REPRODUCTION-2DPAGEIPI00006952.

PTM databases

iPTMnetiQ53H82.
PhosphoSitePlusiQ53H82.

Expressioni

Gene expression databases

BgeeiENSG00000147592.
CleanExiHS_LACTB2.
ExpressionAtlasiQ53H82. baseline and differential.
GenevisibleiQ53H82. HS.

Organism-specific databases

HPAiHPA044391.
HPA053040.

Interactioni

Subunit structurei

Monomer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SIAH1Q8IUQ43EBI-3943430,EBI-747107

Protein-protein interaction databases

BioGridi119299. 20 interactors.
IntActiQ53H82. 2 interactors.
STRINGi9606.ENSP00000276590.

Structurei

Secondary structure

1288
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 10Combined sources4
Beta strandi12 – 19Combined sources8
Turni25 – 27Combined sources3
Beta strandi32 – 36Combined sources5
Beta strandi38 – 45Combined sources8
Helixi53 – 65Combined sources13
Beta strandi70 – 74Combined sources5
Helixi80 – 83Combined sources4
Helixi86 – 92Combined sources7
Beta strandi101 – 103Combined sources3
Turni115 – 118Combined sources4
Beta strandi121 – 123Combined sources3
Beta strandi129 – 132Combined sources4
Beta strandi135 – 141Combined sources7
Beta strandi144 – 146Combined sources3
Beta strandi150 – 154Combined sources5
Turni155 – 158Combined sources4
Beta strandi159 – 163Combined sources5
Beta strandi168 – 170Combined sources3
Helixi177 – 190Combined sources14
Beta strandi193 – 201Combined sources9
Helixi206 – 230Combined sources25
Turni231 – 233Combined sources3
Helixi238 – 245Combined sources8
Turni246 – 248Combined sources3
Helixi251 – 253Combined sources3
Helixi254 – 270Combined sources17
Beta strandi273 – 276Combined sources4
Beta strandi284 – 286Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AD9X-ray2.60A/B/C/D/E/F1-288[»]
ProteinModelPortaliQ53H82.
SMRiQ53H82.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0813. Eukaryota.
COG0491. LUCA.
GeneTreeiENSGT00390000001710.
HOGENOMiHOG000246879.
HOVERGENiHBG099837.
InParanoidiQ53H82.
OMAiHPLAHNQ.
OrthoDBiEOG091G0M3C.
PhylomeDBiQ53H82.
TreeFamiTF314297.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.

Sequencei

Sequence statusi: Complete.

Q53H82-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVLQRVER LSNRVVRVLG CNPGPMTLQG TNTYLVGTGP RRILIDTGEP
60 70 80 90 100
AIPEYISCLK QALTEFNTAI QEIVVTHWHR DHSGGIGDIC KSINNDTTYC
110 120 130 140 150
IKKLPRNPQR EEIIGNGEQQ YVYLKDGDVI KTEGATLRVL YTPGHTDDHM
160 170 180 190 200
ALLLEEENAI FSGDCILGEG TTVFEDLYDY MNSLKELLKI KADIIYPGHG
210 220 230 240 250
PVIHNAEAKI QQYISHRNIR EQQILTLFRE NFEKSFTVME LVKIIYKNTP
260 270 280
ENLHEMAKHN LLLHLKKLEK EGKIFSNTDP DKKWKAHL
Length:288
Mass (Da):32,806
Last modified:January 15, 2008 - v2
Checksum:iF569C7DFB070D52C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti39G → S in BAF82890 (PubMed:14702039).Curated1
Sequence conflicti268L → P in BAD96419 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151841 mRNA. Translation: AAD34078.1.
AK290201 mRNA. Translation: BAF82890.1.
AK222699 mRNA. Translation: BAD96419.1.
CH471068 Genomic DNA. Translation: EAW86969.1.
BC000878 mRNA. Translation: AAH00878.1.
CCDSiCCDS6208.1.
PIRiT44603.
RefSeqiNP_057111.1. NM_016027.2.
UniGeneiHs.118554.

Genome annotation databases

EnsembliENST00000276590; ENSP00000276590; ENSG00000147592.
ENST00000522447; ENSP00000428801; ENSG00000147592.
GeneIDi51110.
KEGGihsa:51110.
UCSCiuc003xyp.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151841 mRNA. Translation: AAD34078.1.
AK290201 mRNA. Translation: BAF82890.1.
AK222699 mRNA. Translation: BAD96419.1.
CH471068 Genomic DNA. Translation: EAW86969.1.
BC000878 mRNA. Translation: AAH00878.1.
CCDSiCCDS6208.1.
PIRiT44603.
RefSeqiNP_057111.1. NM_016027.2.
UniGeneiHs.118554.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AD9X-ray2.60A/B/C/D/E/F1-288[»]
ProteinModelPortaliQ53H82.
SMRiQ53H82.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119299. 20 interactors.
IntActiQ53H82. 2 interactors.
STRINGi9606.ENSP00000276590.

PTM databases

iPTMnetiQ53H82.
PhosphoSitePlusiQ53H82.

Polymorphism and mutation databases

BioMutaiLACTB2.
DMDMi166223244.

2D gel databases

REPRODUCTION-2DPAGEIPI00006952.

Proteomic databases

EPDiQ53H82.
MaxQBiQ53H82.
PaxDbiQ53H82.
PeptideAtlasiQ53H82.
PRIDEiQ53H82.

Protocols and materials databases

DNASUi51110.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000276590; ENSP00000276590; ENSG00000147592.
ENST00000522447; ENSP00000428801; ENSG00000147592.
GeneIDi51110.
KEGGihsa:51110.
UCSCiuc003xyp.4. human.

Organism-specific databases

CTDi51110.
GeneCardsiLACTB2.
HGNCiHGNC:18512. LACTB2.
HPAiHPA044391.
HPA053040.
neXtProtiNX_Q53H82.
OpenTargetsiENSG00000147592.
PharmGKBiPA134952605.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0813. Eukaryota.
COG0491. LUCA.
GeneTreeiENSGT00390000001710.
HOGENOMiHOG000246879.
HOVERGENiHBG099837.
InParanoidiQ53H82.
OMAiHPLAHNQ.
OrthoDBiEOG091G0M3C.
PhylomeDBiQ53H82.
TreeFamiTF314297.

Miscellaneous databases

GenomeRNAii51110.
PROiQ53H82.

Gene expression databases

BgeeiENSG00000147592.
CleanExiHS_LACTB2.
ExpressionAtlasiQ53H82. baseline and differential.
GenevisibleiQ53H82. HS.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLACB2_HUMAN
AccessioniPrimary (citable) accession number: Q53H82
Secondary accession number(s): A8K2D6, Q9Y392
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: November 2, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

RNAi-mediated down-regulation results in rapid cell death. The reason for this is not clear; there are only minor changes in mitochondrial mRNA levels.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.