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Q53H76 (PLA1A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phospholipase A1 member A

EC=3.1.1.-
Alternative name(s):
Phosphatidylserine-specific phospholipase A1
Short name=PS-PLA1
Gene names
Name:PLA1A
Synonyms:NMD, PSPLA1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length456 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolyzes the ester bond at the sn-1 position of glycerophospholipids and produces 2-acyl lysophospholipids. Hydrolyzes phosphatidylserine (PS) in the form of liposomes and 1-acyl-2 lysophosphatidylserine (lyso-PS), but not triolein, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) or phosphatidylinositol (PI). Isoform 2 hydrolyzes lyso-PS but not PS. Hydrolysis of lyso-PS in peritoneal mast cells activated by receptors for IgE leads to stimulate histamine production. Ref.2

Subcellular location

Secreted Potential.

Tissue specificity

Widely expressed. Expressed in placenta, prostate and liver. Weakly or not expressed in skin, leukocytes, platelets, colon, spleen, lung, muscle and kidney. Ref.1

Sequence similarities

Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
   Biological processLipid degradation
Lipid metabolism
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
   Molecular functionHydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processlipid catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

lipid metabolic process

Traceable author statement Ref.2. Source: ProtInc

phosphatidylserine metabolic process

Traceable author statement Ref.2. Source: ProtInc

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionphosphatidylcholine 1-acylhydrolase activity

Traceable author statement Ref.2. Source: ProtInc

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q53H76-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q53H76-2)

Also known as: DeltaC;

The sequence of this isoform differs from the canonical sequence as follows:
     374-376: IPK → MYT
     377-456: Missing.
Isoform 3 (identifier: Q53H76-3)

The sequence of this isoform differs from the canonical sequence as follows:
     136-151: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q53H76-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-173: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 456431Phospholipase A1 member A
PRO_0000273330

Regions

Region374 – 45683Involved in the recognition of diacyl-phospholipids

Sites

Active site1661Nucleophile By similarity
Active site1901Charge relay system By similarity
Active site2601Charge relay system By similarity

Amino acid modifications

Glycosylation791N-linked (GlcNAc...) Potential
Glycosylation3651N-linked (GlcNAc...) Potential
Disulfide bond245 ↔ 258 By similarity
Disulfide bond282 ↔ 293 By similarity
Disulfide bond296 ↔ 304 By similarity

Natural variations

Alternative sequence1 – 173173Missing in isoform 4.
VSP_044944
Alternative sequence136 – 15116Missing in isoform 3.
VSP_022507
Alternative sequence374 – 3763IPK → MYT in isoform 2.
VSP_022508
Alternative sequence377 – 45680Missing in isoform 2.
VSP_022509
Natural variant231S → I.
Corresponds to variant rs11929241 [ dbSNP | Ensembl ].
VAR_030126
Natural variant1101R → H. Ref.8
Corresponds to variant rs61733987 [ dbSNP | Ensembl ].
VAR_030127
Natural variant2841S → N.
Corresponds to variant rs2692622 [ dbSNP | Ensembl ].
VAR_030128

Experimental info

Sequence conflict2131V → T in BAD96425. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: CF09D725FD75820B

FASTA45649,715
        10         20         30         40         50         60 
MPPGPWESCF WVGGLILWLS VGSSGDAPPT PQPKCADFQS ANLFEGTDLK VQFLLFVPSN 

        70         80         90        100        110        120 
PSCGQLVEGS SDLQNSGFNA TLGTKLIIHG FRVLGTKPSW IDTFIRTLLR ATNANVIAVD 

       130        140        150        160        170        180 
WIYGSTGVYF SAVKNVIKLS LEISLFLNKL LVLGVSESSI HIIGVSLGAH VGGMVGQLFG 

       190        200        210        220        230        240 
GQLGQITGLD PAGPEYTRAS VEERLDAGDA LFVEAIHTDT DNLGIRIPVG HVDYFVNGGQ 

       250        260        270        280        290        300 
DQPGCPTFFY AGYSYLICDH MRAVHLYISA LENSCPLMAF PCASYKAFLA GRCLDCFNPF 

       310        320        330        340        350        360 
LLSCPRIGLV EQGGVKIEPL PKEVKVYLLT TSSAPYCMHH SLVEFHLKEL RNKDTNIEVT 

       370        380        390        400        410        420 
FLSSNITSSS KITIPKQQRY GKGIIAHATP QCQINQVKFK FQSSNRVWKK DRTTIIGKFC 

       430        440        450 
TALLPVNDRE KMVCLPEPVN LQASVTVSCD LKIACV 

« Hide

Isoform 2 (DeltaC) [UniParc].

Checksum: E4F6460C73D8F775
Show »

FASTA37640,784
Isoform 3 [UniParc].

Checksum: 3F34DC5D33A8E8C9
Show »

FASTA44047,890
Isoform 4 [UniParc].

Checksum: 1C9E49C66DE3EB7C
Show »

FASTA28331,285

References

« Hide 'large scale' references
[1]"nmd, a novel gene differentially expressed in human melanoma cell lines, encodes a new atypical member of the enzyme family of lipases."
van Groningen J.J.M., Egmond M.R., Bloemers H.P.J., Swart G.W.M.
FEBS Lett. 404:82-86(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Tissue: Melanoma.
[2]"An alternative splicing form of phosphatidylserine-specific phospholipase A1 that exhibits lysophosphatidylserine-specific lysophospholipase activity in humans."
Nagai Y., Aoki J., Sato T., Amano K., Matsuda Y., Arai H., Inoue K.
J. Biol. Chem. 274:11053-11059(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
Tissue: Testis.
[4]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[5]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Skin.
[8]"Polymorphisms in the gene encoding phosphatidylserine-specific phospholipase A1 (PSPLA1)."
Wang J., Wen X.-Y., Stewart A.K., Hegele R.A.
J. Hum. Genet. 47:611-613(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT HIS-110.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U37591 mRNA. Translation: AAC99994.1.
AF035268 mRNA. Translation: AAC98921.1.
AF035269 mRNA. Translation: AAC98922.1.
AK301880 mRNA. Translation: BAG63314.1.
AK313519 mRNA. Translation: BAG36299.1.
AK222705 mRNA. Translation: BAD96425.1.
AC073352 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79557.1.
BC047703 mRNA. Translation: AAH47703.1.
CCDSCCDS2991.1. [Q53H76-1]
CCDS56268.1. [Q53H76-3]
CCDS56269.1. [Q53H76-4]
RefSeqNP_001193889.1. NM_001206960.1. [Q53H76-3]
NP_001193890.1. NM_001206961.1. [Q53H76-4]
NP_056984.1. NM_015900.3. [Q53H76-1]
UniGeneHs.437451.

3D structure databases

ProteinModelPortalQ53H76.
SMRQ53H76. Positions 48-410.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119500. 1 interaction.
IntActQ53H76. 1 interaction.
STRING9606.ENSP00000273371.

PTM databases

PhosphoSiteQ53H76.

Polymorphism databases

DMDM124015212.

Proteomic databases

PaxDbQ53H76.
PRIDEQ53H76.

Protocols and materials databases

DNASU51365.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000273371; ENSP00000273371; ENSG00000144837. [Q53H76-1]
ENST00000488919; ENSP00000420625; ENSG00000144837. [Q53H76-4]
ENST00000495992; ENSP00000417326; ENSG00000144837. [Q53H76-3]
GeneID51365.
KEGGhsa:51365.
UCSCuc003ecu.3. human. [Q53H76-1]
uc003ecv.3. human. [Q53H76-3]

Organism-specific databases

CTD51365.
GeneCardsGC03P119316.
HGNCHGNC:17661. PLA1A.
HPAHPA058981.
MIM607460. gene.
neXtProtNX_Q53H76.
PharmGKBPA134955851.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG39669.
HOGENOMHOG000059578.
HOVERGENHBG082148.
InParanoidQ53H76.
KOK13618.
OMAAPYCVHH.
OrthoDBEOG744T8X.
PhylomeDBQ53H76.
TreeFamTF324997.

Gene expression databases

ArrayExpressQ53H76.
BgeeQ53H76.
CleanExHS_PLA1A.
GenevestigatorQ53H76.

Family and domain databases

Gene3D3.40.50.1820. 1 hit.
InterProIPR029058. AB_hydrolase.
IPR000734. Lipase.
IPR013818. Lipase_N.
IPR016272. Lipoprotein_lipase_LIPH.
[Graphical view]
PANTHERPTHR11610. PTHR11610. 1 hit.
PfamPF00151. Lipase. 1 hit.
[Graphical view]
PIRSFPIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTSPR00821. TAGLIPASE.
SUPFAMSSF53474. SSF53474. 1 hit.
PROSITEPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi51365.
NextBio54833.
PROQ53H76.
SOURCESearch...

Entry information

Entry namePLA1A_HUMAN
AccessionPrimary (citable) accession number: Q53H76
Secondary accession number(s): B2R8V2 expand/collapse secondary AC list , B4DXA2, O95991, Q86WX6, Q9UPD2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM