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Protein

Phospholipase A1 member A

Gene

PLA1A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes the ester bond at the sn-1 position of glycerophospholipids and produces 2-acyl lysophospholipids. Hydrolyzes phosphatidylserine (PS) in the form of liposomes and 1-acyl-2 lysophosphatidylserine (lyso-PS), but not triolein, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) or phosphatidylinositol (PI). Isoform 2 hydrolyzes lyso-PS but not PS. Hydrolysis of lyso-PS in peritoneal mast cells activated by receptors for IgE leads to stimulate histamine production.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei166 – 1661NucleophileBy similarity
Active sitei190 – 1901Charge relay systemPROSITE-ProRule annotation
Active sitei260 – 2601Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

  1. phosphatidylcholine 1-acylhydrolase activity Source: ProtInc

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
  2. lipid metabolic process Source: ProtInc
  3. phosphatidylserine metabolic process Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase A1 member A (EC:3.1.1.-)
Alternative name(s):
Phosphatidylserine-specific phospholipase A1
Short name:
PS-PLA1
Gene namesi
Name:PLA1A
Synonyms:NMD, PSPLA1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:17661. PLA1A.

Subcellular locationi

  1. Secreted Curated

GO - Cellular componenti

  1. acrosomal membrane Source: Ensembl
  2. extracellular vesicular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134955851.

Polymorphism and mutation databases

BioMutaiPLA1A.
DMDMi124015212.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 456431Phospholipase A1 member APRO_0000273330Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi245 ↔ 258By similarity
Disulfide bondi282 ↔ 293By similarity
Disulfide bondi296 ↔ 304By similarity
Glycosylationi365 – 3651N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ53H76.
PRIDEiQ53H76.

PTM databases

PhosphoSiteiQ53H76.

Expressioni

Tissue specificityi

Widely expressed. Expressed in placenta, prostate and liver. Weakly or not expressed in skin, leukocytes, platelets, colon, spleen, lung, muscle and kidney.1 Publication

Gene expression databases

BgeeiQ53H76.
CleanExiHS_PLA1A.
ExpressionAtlasiQ53H76. baseline and differential.
GenevestigatoriQ53H76.

Organism-specific databases

HPAiHPA058981.

Interactioni

Protein-protein interaction databases

BioGridi119500. 1 interaction.
IntActiQ53H76. 1 interaction.
STRINGi9606.ENSP00000273371.

Structurei

3D structure databases

ProteinModelPortaliQ53H76.
SMRiQ53H76. Positions 48-410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni374 – 45683Involved in the recognition of diacyl-phospholipidsAdd
BLAST

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG39669.
GeneTreeiENSGT00760000119069.
HOGENOMiHOG000059578.
HOVERGENiHBG082148.
InParanoidiQ53H76.
KOiK13618.
OMAiAPYCVHH.
OrthoDBiEOG744T8X.
PhylomeDBiQ53H76.
TreeFamiTF324997.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR016272. Lipase_LIPH.
IPR013818. Lipase_N.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PfamiPF00151. Lipase. 1 hit.
[Graphical view]
PIRSFiPIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTSiPR00821. TAGLIPASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q53H76-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPPGPWESCF WVGGLILWLS VGSSGDAPPT PQPKCADFQS ANLFEGTDLK
60 70 80 90 100
VQFLLFVPSN PSCGQLVEGS SDLQNSGFNA TLGTKLIIHG FRVLGTKPSW
110 120 130 140 150
IDTFIRTLLR ATNANVIAVD WIYGSTGVYF SAVKNVIKLS LEISLFLNKL
160 170 180 190 200
LVLGVSESSI HIIGVSLGAH VGGMVGQLFG GQLGQITGLD PAGPEYTRAS
210 220 230 240 250
VEERLDAGDA LFVEAIHTDT DNLGIRIPVG HVDYFVNGGQ DQPGCPTFFY
260 270 280 290 300
AGYSYLICDH MRAVHLYISA LENSCPLMAF PCASYKAFLA GRCLDCFNPF
310 320 330 340 350
LLSCPRIGLV EQGGVKIEPL PKEVKVYLLT TSSAPYCMHH SLVEFHLKEL
360 370 380 390 400
RNKDTNIEVT FLSSNITSSS KITIPKQQRY GKGIIAHATP QCQINQVKFK
410 420 430 440 450
FQSSNRVWKK DRTTIIGKFC TALLPVNDRE KMVCLPEPVN LQASVTVSCD

LKIACV
Length:456
Mass (Da):49,715
Last modified:January 23, 2007 - v2
Checksum:iCF09D725FD75820B
GO
Isoform 2 (identifier: Q53H76-2) [UniParc]FASTAAdd to basket

Also known as: DeltaC

The sequence of this isoform differs from the canonical sequence as follows:
     374-376: IPK → MYT
     377-456: Missing.

Show »
Length:376
Mass (Da):40,784
Checksum:iE4F6460C73D8F775
GO
Isoform 3 (identifier: Q53H76-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     136-151: Missing.

Note: No experimental confirmation available.

Show »
Length:440
Mass (Da):47,890
Checksum:i3F34DC5D33A8E8C9
GO
Isoform 4 (identifier: Q53H76-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-173: Missing.

Note: No experimental confirmation available.

Show »
Length:283
Mass (Da):31,285
Checksum:i1C9E49C66DE3EB7C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti213 – 2131V → T in BAD96425 (Ref. 4) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti23 – 231S → I.
Corresponds to variant rs11929241 [ dbSNP | Ensembl ].
VAR_030126
Natural varianti110 – 1101R → H.1 Publication
Corresponds to variant rs61733987 [ dbSNP | Ensembl ].
VAR_030127
Natural varianti284 – 2841S → N.
Corresponds to variant rs2692622 [ dbSNP | Ensembl ].
VAR_030128

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 173173Missing in isoform 4. 1 PublicationVSP_044944Add
BLAST
Alternative sequencei136 – 15116Missing in isoform 3. 1 PublicationVSP_022507Add
BLAST
Alternative sequencei374 – 3763IPK → MYT in isoform 2. 1 PublicationVSP_022508
Alternative sequencei377 – 45680Missing in isoform 2. 1 PublicationVSP_022509Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37591 mRNA. Translation: AAC99994.1.
AF035268 mRNA. Translation: AAC98921.1.
AF035269 mRNA. Translation: AAC98922.1.
AK301880 mRNA. Translation: BAG63314.1.
AK313519 mRNA. Translation: BAG36299.1.
AK222705 mRNA. Translation: BAD96425.1.
AC073352 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79557.1.
BC047703 mRNA. Translation: AAH47703.1.
CCDSiCCDS2991.1. [Q53H76-1]
CCDS56268.1. [Q53H76-3]
CCDS56269.1. [Q53H76-4]
RefSeqiNP_001193889.1. NM_001206960.1. [Q53H76-3]
NP_001193890.1. NM_001206961.1. [Q53H76-4]
NP_056984.1. NM_015900.3. [Q53H76-1]
UniGeneiHs.437451.

Genome annotation databases

EnsembliENST00000273371; ENSP00000273371; ENSG00000144837. [Q53H76-1]
ENST00000488919; ENSP00000420625; ENSG00000144837. [Q53H76-4]
ENST00000495992; ENSP00000417326; ENSG00000144837. [Q53H76-3]
GeneIDi51365.
KEGGihsa:51365.
UCSCiuc003ecu.3. human. [Q53H76-1]
uc003ecv.3. human. [Q53H76-3]

Polymorphism and mutation databases

BioMutaiPLA1A.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37591 mRNA. Translation: AAC99994.1.
AF035268 mRNA. Translation: AAC98921.1.
AF035269 mRNA. Translation: AAC98922.1.
AK301880 mRNA. Translation: BAG63314.1.
AK313519 mRNA. Translation: BAG36299.1.
AK222705 mRNA. Translation: BAD96425.1.
AC073352 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79557.1.
BC047703 mRNA. Translation: AAH47703.1.
CCDSiCCDS2991.1. [Q53H76-1]
CCDS56268.1. [Q53H76-3]
CCDS56269.1. [Q53H76-4]
RefSeqiNP_001193889.1. NM_001206960.1. [Q53H76-3]
NP_001193890.1. NM_001206961.1. [Q53H76-4]
NP_056984.1. NM_015900.3. [Q53H76-1]
UniGeneiHs.437451.

3D structure databases

ProteinModelPortaliQ53H76.
SMRiQ53H76. Positions 48-410.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119500. 1 interaction.
IntActiQ53H76. 1 interaction.
STRINGi9606.ENSP00000273371.

PTM databases

PhosphoSiteiQ53H76.

Polymorphism and mutation databases

BioMutaiPLA1A.
DMDMi124015212.

Proteomic databases

PaxDbiQ53H76.
PRIDEiQ53H76.

Protocols and materials databases

DNASUi51365.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000273371; ENSP00000273371; ENSG00000144837. [Q53H76-1]
ENST00000488919; ENSP00000420625; ENSG00000144837. [Q53H76-4]
ENST00000495992; ENSP00000417326; ENSG00000144837. [Q53H76-3]
GeneIDi51365.
KEGGihsa:51365.
UCSCiuc003ecu.3. human. [Q53H76-1]
uc003ecv.3. human. [Q53H76-3]

Organism-specific databases

CTDi51365.
GeneCardsiGC03P119316.
HGNCiHGNC:17661. PLA1A.
HPAiHPA058981.
MIMi607460. gene.
neXtProtiNX_Q53H76.
PharmGKBiPA134955851.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG39669.
GeneTreeiENSGT00760000119069.
HOGENOMiHOG000059578.
HOVERGENiHBG082148.
InParanoidiQ53H76.
KOiK13618.
OMAiAPYCVHH.
OrthoDBiEOG744T8X.
PhylomeDBiQ53H76.
TreeFamiTF324997.

Miscellaneous databases

GenomeRNAii51365.
NextBioi54833.
PROiQ53H76.
SOURCEiSearch...

Gene expression databases

BgeeiQ53H76.
CleanExiHS_PLA1A.
ExpressionAtlasiQ53H76. baseline and differential.
GenevestigatoriQ53H76.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR016272. Lipase_LIPH.
IPR013818. Lipase_N.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PfamiPF00151. Lipase. 1 hit.
[Graphical view]
PIRSFiPIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTSiPR00821. TAGLIPASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "nmd, a novel gene differentially expressed in human melanoma cell lines, encodes a new atypical member of the enzyme family of lipases."
    van Groningen J.J.M., Egmond M.R., Bloemers H.P.J., Swart G.W.M.
    FEBS Lett. 404:82-86(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Melanoma.
  2. "An alternative splicing form of phosphatidylserine-specific phospholipase A1 that exhibits lysophosphatidylserine-specific lysophospholipase activity in humans."
    Nagai Y., Aoki J., Sato T., Amano K., Matsuda Y., Arai H., Inoue K.
    J. Biol. Chem. 274:11053-11059(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
    Tissue: Testis.
  4. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  5. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Skin.
  8. "Polymorphisms in the gene encoding phosphatidylserine-specific phospholipase A1 (PSPLA1)."
    Wang J., Wen X.-Y., Stewart A.K., Hegele R.A.
    J. Hum. Genet. 47:611-613(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT HIS-110.

Entry informationi

Entry nameiPLA1A_HUMAN
AccessioniPrimary (citable) accession number: Q53H76
Secondary accession number(s): B2R8V2
, B4DXA2, O95991, Q86WX6, Q9UPD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: January 23, 2007
Last modified: April 29, 2015
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.