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Protein

tRNA wybutosine-synthesizing protein 2 homolog

Gene

TRMT12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent transferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14) to produce wybutosine-86.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + 4-demethylwyosine(37) in tRNA(Phe) = S-methyl-5'-thioadenosine + 7-((3S)-3-amino-3-carboxypropyl)-4-demethylwyosine(37) in tRNA(Phe).1 Publication

Pathwayi: wybutosine-tRNA(Phe) biosynthesis

This protein is involved in the pathway wybutosine-tRNA(Phe) biosynthesis, which is part of tRNA modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway wybutosine-tRNA(Phe) biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei218S-adenosyl-L-methionine1
Binding sitei225S-adenosyl-L-methionine1
Binding sitei265S-adenosyl-L-methionine1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.5.1.114. 2681.
ReactomeiR-HSA-6782861. Synthesis of wybutosine at G37 of tRNA(Phe).
UniPathwayiUPA00375.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA wybutosine-synthesizing protein 2 homolog (EC:2.5.1.114)
Short name:
tRNA-yW-synthesizing protein 2
Alternative name(s):
tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase
Gene namesi
Name:TRMT12
Synonyms:TRM12, TYW2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000183665.5.
HGNCiHGNC:26091. TRMT12.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi225K → A: Lack of activity. 1 Publication1
Mutagenesisi242Y → A: Does not affect activity. 1 Publication1
Mutagenesisi248F → A: Does not affect activity. 1 Publication1
Mutagenesisi265E → A: Lack of activity. 1 Publication1
Mutagenesisi293D → A: Does not affect activity. 1 Publication1

Organism-specific databases

DisGeNETi55039.
OpenTargetsiENSG00000183665.
PharmGKBiPA142670700.

Polymorphism and mutation databases

BioMutaiTRMT12.
DMDMi74726289.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002818361 – 448tRNA wybutosine-synthesizing protein 2 homologAdd BLAST448

Proteomic databases

EPDiQ53H54.
MaxQBiQ53H54.
PaxDbiQ53H54.
PeptideAtlasiQ53H54.
PRIDEiQ53H54.

PTM databases

iPTMnetiQ53H54.
PhosphoSitePlusiQ53H54.

Expressioni

Gene expression databases

BgeeiENSG00000183665.
CleanExiHS_TRMT12.
ExpressionAtlasiQ53H54. baseline and differential.
GenevisibleiQ53H54. HS.

Organism-specific databases

HPAiHPA023939.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
LNX1Q8TBB13EBI-10242598,EBI-739832

Protein-protein interaction databases

BioGridi120368. 6 interactors.
IntActiQ53H54. 1 interactor.
STRINGi9606.ENSP00000329858.

Structurei

3D structure databases

ProteinModelPortaliQ53H54.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni293 – 294S-adenosyl-L-methionine binding2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.

Phylogenomic databases

eggNOGiKOG1227. Eukaryota.
COG2520. LUCA.
GeneTreeiENSGT00390000006078.
HOGENOMiHOG000294185.
HOVERGENiHBG108624.
InParanoidiQ53H54.
KOiK07055.
OMAiHIQPVKS.
OrthoDBiEOG091G06O6.
PhylomeDBiQ53H54.
TreeFamiTF314137.

Family and domain databases

InterProiView protein in InterPro
IPR030382. MeTrfase_TRM5/TYW2.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF02475. Met_10. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiView protein in PROSITE
PS51684. SAM_MT_TRM5_TYW2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q53H54-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRENVVVSNM ERESGKPVAV VAVVTEPWFT QRYREYLQRQ KLFDTQHRVE
60 70 80 90 100
KMPDGSVALP VLGETLPEQH LQELRNRVAP GSPCMLTQLP DPVPSKRAQG
110 120 130 140 150
CSPAQKLCLE VSRWVEGRGV KWSAELEADL PRSWQRHGNL LLLSEDCFQA
160 170 180 190 200
KQWKNLGPEL WETVALALGV QRLAKRGRVS PDGTRTPAVT LLLGDHGWVE
210 220 230 240 250
HVDNGIRYKF DVTQCMFSFG NITEKLRVAS LSCAGEVLVD LYAGIGYFTL
260 270 280 290 300
PFLVHAGAAF VHACEWNPHA VVALRNNLEI NGVADRCQIH FGDNRKLKLS
310 320 330 340 350
NIADRVILGL IPSSEEGWPI ACQVLRQDAG GILHIHQNVE SFPGKNLQAL
360 370 380 390 400
GVSKVEKEHW LYPQQITTNQ WKNGATRDSR GKMLSPATKP EWQRWAESAE
410 420 430 440
TRIATLLQQV HGKPWKTQIL HIQPVKSYAP HVDHIVLDLE CCPCPSVG
Length:448
Mass (Da):50,236
Last modified:May 24, 2005 - v1
Checksum:i5E66C20AF26C4B5F
GO

Sequence cautioni

The sequence AAH03057 differs from that shown. Contaminating sequence. Potential poly-A sequence starting in position 371.Curated
The sequence AAH03057 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti342F → S in BAA91374 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03129128W → R1 PublicationCorresponds to variant dbSNP:rs3812475Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000779 mRNA. Translation: BAA91374.1.
AK222727 mRNA. Translation: BAD96447.1.
BC003057 mRNA. Translation: AAH03057.2. Sequence problems.
BC011713 mRNA. Translation: AAH11713.1.
CCDSiCCDS6349.1.
RefSeqiNP_060426.2. NM_017956.3.
UniGeneiHs.9925.

Genome annotation databases

EnsembliENST00000328599; ENSP00000329858; ENSG00000183665.
GeneIDi55039.
KEGGihsa:55039.
UCSCiuc003yra.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTYW2_HUMAN
AccessioniPrimary (citable) accession number: Q53H54
Secondary accession number(s): Q6PKB9, Q96F21, Q9NWK6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: May 24, 2005
Last modified: October 25, 2017
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families