Q53H54 (TYW2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA wybutosine-synthesizing protein 2 homolog
Short name=tRNA-yW-synthesizing protein 2

Alternative name(s):
Alpha-amino-alpha-carboxypropyl transferase TYW2
EC=2.5.1.-

Gene names

Name:	TRMT12
Synonyms:	TRM12, TYW2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable S-adenosyl-L-methionine-dependent transferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA By similarity.
Pathway	tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
Sequence similarities	Belongs to the TRM5 / TYW2 family.
Sequence caution	The sequence AAH03057.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAH03057.2 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence starting in position 371.

Ontologies

Keywords
Biological process	tRNA processing
Coding sequence diversity	Polymorphism
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	tRNA wybutosine-synthesizing protein 2 homolog		PRO_0000281836
Regions
	Region	293 – 294	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	218	1	S-adenosyl-L-methionine By similarity
	Binding site	225	1	S-adenosyl-L-methionine By similarity
	Binding site	265	1	S-adenosyl-L-methionine By similarity
Natural variations
	Natural variant	28	1	W → R. Ref.3 Corresponds to variant rs3812475 [ dbSNP | Ensembl ].		VAR_031291
Experimental info
	Sequence conflict	342	1	F → S in BAA91374. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q53H54 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 5E66C20AF26C4B5F	FASTA	448	50,236
	10 20 30 40 50 60 MRENVVVSNM ERESGKPVAV VAVVTEPWFT QRYREYLQRQ KLFDTQHRVE KMPDGSVALP 70 80 90 100 110 120 VLGETLPEQH LQELRNRVAP GSPCMLTQLP DPVPSKRAQG CSPAQKLCLE VSRWVEGRGV 130 140 150 160 170 180 KWSAELEADL PRSWQRHGNL LLLSEDCFQA KQWKNLGPEL WETVALALGV QRLAKRGRVS 190 200 210 220 230 240 PDGTRTPAVT LLLGDHGWVE HVDNGIRYKF DVTQCMFSFG NITEKLRVAS LSCAGEVLVD 250 260 270 280 290 300 LYAGIGYFTL PFLVHAGAAF VHACEWNPHA VVALRNNLEI NGVADRCQIH FGDNRKLKLS 310 320 330 340 350 360 NIADRVILGL IPSSEEGWPI ACQVLRQDAG GILHIHQNVE SFPGKNLQAL GVSKVEKEHW 370 380 390 400 410 420 LYPQQITTNQ WKNGATRDSR GKMLSPATKP EWQRWAESAE TRIATLLQQV HGKPWKTQIL 430 440 HIQPVKSYAP HVDHIVLDLE CCPCPSVG

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.
[2]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-28. Tissue: Ovary and Skin.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK000779 mRNA. Translation: BAA91374.1. AK222727 mRNA. Translation: BAD96447.1. BC003057 mRNA. Translation: AAH03057.2. Sequence problems. BC011713 mRNA. Translation: AAH11713.1.
IPI	IPI00329723.
RefSeq	NP_060426.2. NM_017956.3.
UniGene	Hs.9925.
3D structure databases
ProteinModelPortal	Q53H54.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000329858.
Polymorphism databases
DMDM	74726289.
Proteomic databases
PaxDb	Q53H54.
PeptideAtlas	Q53H54.
PRIDE	Q53H54.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000328599; ENSP00000329858; ENSG00000183665.
GeneID	55039.
KEGG	hsa:55039.
UCSC	uc003yra.4. human.
Organism-specific databases
CTD	55039.
GeneCards	GC08P125463.
HGNC	HGNC:26091. TRMT12.
HPA	HPA023939.
MIM	611244. gene.
neXtProt	NX_Q53H54.
PharmGKB	PA142670700.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2520.
HOGENOM	HOG000294185.
HOVERGEN	HBG108624.
InParanoid	Q53H54.
KO	K07055.
OMA	HFGDNRK.
OrthoDB	EOG4X0MS6.
Enzyme and pathway databases
UniPathway	UPA00375.
Gene expression databases
ArrayExpress	Q53H54.
Bgee	Q53H54.
CleanEx	HS_TRMT12.
Genevestigator	Q53H54.
Family and domain databases
InterPro	IPR003402. tRNA_Trfase_Trm5/Tyw2. [Graphical view]
Pfam	PF02475. Met_10. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TRMT12. human.
GenomeRNAi	55039.
NextBio	58491.
SOURCE	Search...

Entry information

Entry name

TYW2_HUMAN

Accession

Primary (citable) accession number: Q53H54
Secondary accession number(s): Q6PKB9, Q96F21, Q9NWK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	May 24, 2005
Last modified:	May 29, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.