Reviewed, UniProtKB/Swiss-Prot Q53GI3 (ZN394_HUMAN)

Names and origin

Protein names

Recommended name:
    Zinc finger protein 394
Alternative name(s):
    Zinc finger protein with KRAB and SCAN domains 14

Gene names

Name:	ZNF394
Synonyms:	ZKSCAN14

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May be involved in transcriptional regulation.
Subcellular location	Nucleus Potential.
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 7 C2H2-type zinc fingers. Contains 1 KRAB domain. Contains 1 SCAN box domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	Zinc finger protein 394		PRO_0000047557
Regions
	Domain	64 – 146	83	SCAN box
	Domain	155 – 230	76	KRAB
	Zinc finger	358 – 380	23	C2H2-type 1
	Zinc finger	386 – 408	23	C2H2-type 2
	Zinc finger	414 – 436	23	C2H2-type 3
	Zinc finger	442 – 463	22	C2H2-type 4
	Zinc finger	469 – 491	23	C2H2-type 5
	Zinc finger	497 – 519	23	C2H2-type 6
	Zinc finger	525 – 547	23	C2H2-type 7
Natural variations
	Natural variant	325	1	T → M: dbSNP rs3735454.		VAR_052816
Experimental info
	Sequence conflict	3	1	S → C in BAB14021. Ref.2
	Sequence conflict	21	1	M → T in BAD96668. Ref.3
	Sequence conflict	127	1	H → Y Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q53GI3-1 [UniParc]. Last modified November 8, 2005. Version 2. Checksum: D92AE5E58DEDAAA5	FASTA	561	64,256
	10 20 30 40 50 60 MNSSLTAQRR GSDAELGPWV MAARSKDAAP SQRDGLLPVK VEEDSPGSWE PNYPAASPDP 70 80 90 100 110 120 ETSRLHFRQL RYQEVAGPEE ALSRLRELCR RWLRPELLSK EQILELLVLE QFLTILPEEL 130 140 150 160 170 180 QAWVREHCPE SGEEAVAVVR ALQRALDGTS SQGMVTFEDT AVSLTWEEWE RLDPARRDFC 190 200 210 220 230 240 RESAQKDSGS TVPPSLESRV ENKELIPMQQ ILEEAEPQGQ LQEAFQGKRP LFSKCGSTHE 250 260 270 280 290 300 DRVEKQSGDP LPLKLENSPE AEGLNSISDV NKNGSIEGED SKNNELQNSA RCSNLVLCQH 310 320 330 340 350 360 IPKAERPTDS EEHGNKCKQS FHMVTWHVLK PHKSDSGDSF HHSSLFETQR QLHEERPYKC 370 380 390 400 410 420 GNCGKSFKQR SDLFRHQRIH TGEKPYGCQE CGKSFSQSAA LTKHQRTHTG EKPYTCLKCG 430 440 450 460 470 480 ERFRQNSHLN RHQSTHSRDK HFKCEECGET CHISNLFRHQ RLHKGERPYK CEECEKSFKQ 490 500 510 520 530 540 RSDLFKHHRI HTGEKPYGCS VCGKRFNQSA TLIKHQRIHT GEKPYKCLEC GERFRQSTHL 550 560 IRHQRIHQNK VLSAGRGGSR L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Inhibition of transcriptional activities of AP-1 and c-Jun by a new zinc finger protein ZNF394." Huang C., Wang Y., Li D., Li Y., Luo J., Yuan W., Ou Y., Zhu C., Zhang Y., Wang Z., Liu M., Wu X. Biochem. Biophys. Res. Commun. 320:1298-1305(2004) [PubMed: 15249231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary gland.
[3]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[4]	"Human chromosome 7: DNA sequence and biology." Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C. Science 300:767-772(2003) [PubMed: 12690205] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Skin.
[7]	"Repression of transcriptional activity at a distance by the evolutionarily conserved KRAB domain present in a subfamily of zinc finger proteins." Pengue G., Calabro V., Bartoli P.C., Pagliuca A., Lania L. Nucleic Acids Res. 22:2908-2914(1994) [PubMed: 8065901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 67-143.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY642122 mRNA. Translation: AAT67053.1. AK022360 mRNA. Translation: BAB14021.1. AK222948 mRNA. Translation: BAD96668.1. CH236956 Genomic DNA. Translation: EAL23874.1. CH471091 Genomic DNA. Translation: EAW76659.1. BC025241 mRNA. Translation: AAH25241.1. BC051847 mRNA. Translation: AAH51847.1.
IPI	IPI00550333.
RefSeq	NP_115540.2.
UniGene	Hs.386324
3D structure databases
SMR	Q53GI3. Positions 58-145, 148-192, 355-521, 383-548.
ModBase	Search...
Proteomic databases
PRIDE	Q53GI3.
Genome annotation databases
Ensembl	ENST00000337673; ENSP00000337363; ENSG00000160908; Homo sapiens. [Genome view] ENST00000426306; ENSP00000409565; ENSG00000160908; Homo sapiens. [Genome view] ENST00000429454; ENSP00000397804; ENSG00000160908; Homo sapiens. [Genome view]
GeneID	84124.
KEGG	hsa:84124.
UCSC	uc003uqs.1. human.
Organism-specific databases
CTD	84124.
GeneCards	GC07M098928.
HGNC	HGNC:18832. ZNF394.
PharmGKB	PA38702.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG717200.
HOVERGEN	Q53GI3.
InParanoid	Q53GI3.
OMA	EDSKNNE.
OrthoDB	EOG9323JD.
PhylomeDB	Q53GI3.
Gene expression databases
Bgee	Q53GI3.
CleanEx	HS_ZNF394.
Genevestigator	Q53GI3.
GermOnline	ENSG00000160908. Homo sapiens.
Family and domain databases
InterPro	IPR001909. Krueppel-associated_box. IPR003309. Treg_SCAN. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 6 hits.
Pfam	PF01352. KRAB. 1 hit. PF02023. SCAN. 1 hit. PF00096. zf-C2H2. 6 hits. [Graphical view]
SMART	SM00349. KRAB. 1 hit. SM00431. SCAN. 1 hit. SM00355. ZnF_C2H2. 7 hits. [Graphical view]
PROSITE	PS50805. KRAB. 1 hit. PS50804. SCAN_BOX. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 6 hits. PS50157. ZINC_FINGER_C2H2_2. 7 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	73385.

Entry information

Entry name

ZN394_HUMAN

Accession

Primary (citable) accession number: Q53GI3
Secondary accession number(s): A4D281 , Q6P5X9, Q8TB27, Q9HA37, Q9UD51

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	January 19, 2010
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.