Reviewed, UniProtKB/Swiss-Prot Q53FA7 (QORX_HUMAN)

Names and origin

Protein names

Recommended name:
    Putative quinone oxidoreductase
    EC=1.-.-.-
Alternative name(s):
    Tumor protein p53-inducible protein 3
    p53-induced gene 3 protein

Gene names

Name:	TP53I3
Synonyms:	PIG3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in the generation of reactive oxygen species (ROS).
Induction	Isoforms 1 and 2 are both activated by TP53/p53, doxorubicin, etoposide and ionizing radiation. Isoform 2 is highly activated by UV radiation. Ref.1
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
Sequence caution	The sequence AAC39535.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	induction of apoptosis by oxidative stress Non-traceable author statement. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: UV radiation favors the production of isoform 2.

Isoform 1 (identifier: Q53FA7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Major isoform under normal light conditions.

Isoform 2 (identifier: Q53FA7-2)

Also known as: PIG3AS;

The sequence of this isoform differs from the canonical sequence as follows:
     207-248: GAGVNLILDC...GLMGGGDING → VQANAGECFH...LPSDRNPGGP
     249-332: Missing.

Note: Major isoform under UV light exposure. Undergoes rapid proteolytic degradation by the proteasome.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 332

332

Putative quinone oxidoreductase

PRO_0000160917

Amino acid modifications

Modified residue

1

1

N-acetylmethionine Ref.8

Natural variations

Alternative sequence

207 – 248

42

GAGVN…GDING → VQANAGECFHGANSASLLHG GPPTSAAGSGQNLPSDRNPG GP in isoform 2.

VSP_015783

Alternative sequence

249 – 332

84

Missing in isoform 2.

VSP_015784

Natural variant

180

1

M → K in a breast cancer sample; somatic mutation. Ref.9

VAR_033032

Natural variant

223

1

E → K: dbSNP rs35176319.

VAR_048201

Experimental info

Sequence conflict

36

1

A → AA in AAC39535. Ref.1

Sequence conflict

263

1

T → A in BAD97102. Ref.4

Sequence conflict

315 – 332

18

KYMEA…LELPQ → STWRPTRT in AAC39528. Ref.1

Secondary structure

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332

Helix Strand Turn

Details...

Beta strand

1 – 8

8

Helix

12 – 14

3

Beta strand

15 – 21

7

Beta strand

29 – 38

10

Helix

41 – 47

7

Beta strand

59 – 61

3

Beta strand

63 – 72

10

Beta strand

85 – 89

5

Beta strand

95 – 102

8

Helix

103 – 105

3

Beta strand

106 – 108

3

Helix

115 – 118

4

Helix

122 – 132

11

Turn

133 – 135

3

Beta strand

143 – 148

6

Helix

152 – 163

12

Beta strand

167 – 173

7

Helix

175 – 184

10

Beta strand

187 – 191

5

Turn

192 – 194

3

Helix

197 – 204

8

Turn

205 – 207

3

Beta strand

210 – 217

8

Helix

219 – 221

3

Helix

222 – 228

7

Beta strand

229 – 237

9

Beta strand

246 – 248

3

Helix

250 – 256

7

Beta strand

260 – 263

4

Helix

271 – 284

14

Helix

286 – 289

4

Beta strand

301 – 306

6

Helix

307 – 309

3

Helix

310 – 318

9

Beta strand

323 – 329

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 27, 2005. Version 2. Checksum: C5A33C46B3F96473	FASTA	332	35,536
	10 20 30 40 50 60 MLAVHFDKPG GPENLYVKEV AKPSPGEGEV LLKVAASALN RADLMQRQGQ YDPPPGASNI 70 80 90 100 110 120 LGLEASGHVA ELGPGCQGHW KIGDTAMALL PGGGQAQYVT VPEGLLMPIP EGLTLTQAAA 130 140 150 160 170 180 IPEAWLTAFQ LLHLVGNVQA GDYVLIHAGL SGVGTAAIQL TRMAGAIPLV TAGSQKKLQM 190 200 210 220 230 240 AEKLGAAAGF NYKKEDFSEA TLKFTKGAGV NLILDCIGGS YWEKNVNCLA LDGRWVLYGL 250 260 270 280 290 300 MGGGDINGPL FSKLLFKRGS LITSLLRSRD NKYKQMLVNA FTEQILPHFS TEGPQRLLPV 310 320 330 LDRIYPVTEI QEAHKYMEAN KNIGKIVLEL PQ
	Isoform 2 (PIG3AS). Checksum: 8899613C763DDDDC	FASTA	248	25,402
	10 20 30 40 50 60 MLAVHFDKPG GPENLYVKEV AKPSPGEGEV LLKVAASALN RADLMQRQGQ YDPPPGASNI 70 80 90 100 110 120 LGLEASGHVA ELGPGCQGHW KIGDTAMALL PGGGQAQYVT VPEGLLMPIP EGLTLTQAAA 130 140 150 160 170 180 IPEAWLTAFQ LLHLVGNVQA GDYVLIHAGL SGVGTAAIQL TRMAGAIPLV TAGSQKKLQM 190 200 210 220 230 240 AEKLGAAAGF NYKKEDFSEA TLKFTKVQAN AGECFHGANS ASLLHGGPPT SAAGSGQNLP SDRNPGGP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A model for p53-induced apoptosis." Polyak K., Xia Y., Zweier J.L., Kinzler K.W., Vogelstein B. Nature 389:300-306(1997) [PubMed: 9305847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), INDUCTION BY TP53. Tissue: Colon cancer.
[2]	"UV-dependent alternative splicing uncouples p53 activity and PIG3 gene function through rapid proteolytic degradation." Nicholls C.D., Shields M.A., Lee P.W.K., Robbins S.M., Beattie T.L. J. Biol. Chem. 279:24171-24178(2004) [PubMed: 15067011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Mammary tumor.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Gastric mucosa.
[5]	NIEHS SNPs program Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Muscle.
[8]	Bienvenut W.V., Vousden K.H., Lukashchuk N. Submitted (MAR-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-33; 163-176; 184-203; 259-267; 297-303 AND 326-332, ACETYLATION AT MET-1, MASS SPECTROMETRY. Tissue: Lung carcinoma.
[9]	"Somatic sequence alterations in twenty-one genes selected by expression profile analysis of breast carcinomas." Chanock S.J., Burdett L., Yeager M., Llaca V., Langeroed A., Presswalla S., Kaaresen R., Strausberg R.L., Gerhard D.S., Kristensen V., Perou C.M., Boerresen-Dale A.-L. Breast Cancer Res. 9:R5-R5(2007) [PubMed: 17224074] [Abstract] Cited for: VARIANT LYS-180.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF010309 mRNA. Translation: AAC39528.1.
AF010317 Genomic DNA. Translation: AAC39535.1. Sequence problems.
AY371700 mRNA. Translation: AAQ90166.1.
BT007149 mRNA. Translation: AAP35813.1.
AK223382 mRNA. Translation: BAD97102.1.
DQ232851 Genomic DNA. Translation: ABB02183.1.
AC008073 Genomic DNA. Translation: AAY14665.1.
BC000474 mRNA. Translation: AAH00474.1.

IPI

IPI00384643.
IPI00418440.

RefSeq

NP_004872.2.
NP_671713.1.

UniGene

Hs.50649

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2J8Z	X-ray	2.50	A	1-332	[»]
2OBY	X-ray	3.00	A/B/C/D/E	1-332	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q53FA7. 4 interactions.

PTM databases

PhosphoSite

Q53FA7.

Proteomic databases

PeptideAtlas

Q53FA7.

PRIDE

Q53FA7.

Genome annotation databases

Ensembl

ENSG00000115129. Homo sapiens. [Contig view]

GeneID

9540.

KEGG

hsa:9540.

Organism-specific databases

GeneCards

GC02M024211.

HGNC

HGNC:19373. TP53I3.

MIM

605171. gene.

PharmGKB

PA134923704.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q53FA7.

HOVERGEN

Q53FA7.

OMA

Q53FA7. SEATLKF.

Gene expression databases

ArrayExpress

Q53FA7.

Bgee

Q53FA7.

CleanEx

HS_TP53I3.

GermOnline

ENSG00000115129. Homo sapiens.

Family and domain databases

InterPro

IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn.
IPR013149. ADH_Zn-bd.
IPR016040. NAD(P)-bd_dom.
IPR002364. Quin_OxRdtase/zeta-crystal_CS.
IPR014189. Quinone_OxRdtase_PIG3.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

PANTHER

PTHR11695. ADH_Sf_Zn. 1 hit.
PTHR11695:SF28. Quinone_oxidored_PIG3. 1 hit.

Pfam

PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02824. quinone_pig3. 1 hit.

PROSITE

PS01162. QOR_ZETA_CRYSTAL. False negative.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

35770.

SOURCE

Search...

Entry information

Entry name

QORX_HUMAN

Accession

Primary (citable) accession number: Q53FA7
Secondary accession number(s): O14679 , O14685, Q38G78, Q6JLE7, Q9BWB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	September 27, 2005
Last modified:	June 16, 2009
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)