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Protein

Nuclear cap-binding protein subunit 3

Gene

NCBP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Associates with NCBP1/CBP80 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export. NCBP3 serves as adapter protein linking the capped RNAs (m7GpppG-capped RNA) to NCBP1/CBP80. Unlike the conventional CBC with NCBP2 which binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus, the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role in only mRNA export. The alternative CBC is particularly important in cellular stress situations such as virus infections and the NCBP3 activity is critical to inhibit virus growth (PubMed:26382858).1 Publication

GO - Molecular functioni

  • mRNA binding Source: UniProtKB
  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB
  • RNA 7-methylguanosine cap binding Source: UniProtKB

GO - Biological processi

  • 7-methylguanosine mRNA capping Source: UniProtKB-KW
  • defense response to virus Source: UniProtKB
  • mRNA transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Antiviral defense, mRNA capping, mRNA processing, mRNA transport, Transport

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear cap-binding protein subunit 3Imported
Alternative name(s):
Protein ELG
Gene namesi
Name:NCBP3Imported
Synonyms:C17orf85
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:24612. NCBP3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
  • RNA cap binding complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi134D → A: Minor loss of 7-methylguanosine-containing mRNA cap binding. 1 Publication1
Mutagenesisi155 – 158WLDD → ALAA: Complete loss of 7-methylguanosine-containing mRNA cap binding. 1 Publication4

Organism-specific databases

OpenTargetsiENSG00000074356.
PharmGKBiPA145149545.

Polymorphism and mutation databases

BioMutaiC17orf85.
DMDMi160017360.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003085821 – 620Nuclear cap-binding protein subunit 3Add BLAST620

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki12Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei25PhosphoserineCombined sources1
Modified residuei73PhosphoserineCombined sources1
Modified residuei209PhosphoserineCombined sources1
Modified residuei210PhosphoserineCombined sources1
Modified residuei413PhosphothreonineCombined sources1
Modified residuei415PhosphoserineCombined sources1
Modified residuei620PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ53F19.
MaxQBiQ53F19.
PaxDbiQ53F19.
PeptideAtlasiQ53F19.
PRIDEiQ53F19.

PTM databases

iPTMnetiQ53F19.
PhosphoSitePlusiQ53F19.

Expressioni

Gene expression databases

BgeeiENSG00000074356.
CleanExiHS_C17orf85.
ExpressionAtlasiQ53F19. baseline and differential.
GenevisibleiQ53F19. HS.

Organism-specific databases

HPAiHPA008959.
HPA013195.

Interactioni

Subunit structurei

Component of an alternative cap-binding complex (CBC) composed of NCBP1/CBP80 and NCBP3. Interacts with SRRT, KPNA3, THOC5 and EIF4A3.1 Publication

Protein-protein interaction databases

BioGridi120662. 48 interactors.
IntActiQ53F19. 29 interactors.
MINTiMINT-8332606.
STRINGi9606.ENSP00000373657.

Structurei

3D structure databases

ProteinModelPortaliQ53F19.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni126 – 187RNA recognition motif (RRM) domain1 PublicationAdd BLAST62

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi155 – 158WLDD motif; essential for 7-methylguanosine-containing mRNA cap binding1 Publication4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi340 – 376Glu-richAdd BLAST37

Sequence similaritiesi

Belongs to the NCBP3 family.Curated

Phylogenomic databases

eggNOGiENOG410IHKG. Eukaryota.
ENOG4110MQP. LUCA.
GeneTreeiENSGT00390000005712.
HOGENOMiHOG000059260.
HOVERGENiHBG059983.
InParanoidiQ53F19.
OMAiIFSYFKE.
OrthoDBiEOG091G0ELU.
PhylomeDBiQ53F19.
TreeFamiTF331339.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR019416. NCBP3.
IPR012677. Nucleotide-bd_a/b_plait.
[Graphical view]
PANTHERiPTHR16291. PTHR16291. 2 hits.
PfamiPF10309. NCBP3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q53F19-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAVRGLRVS VKAEAPAGPA LGLPSPEAES GVDRGEPEPM EVEEGELEIV
60 70 80 90 100
PVRRSLKELI PDTSRRYENK AGSFITGIDV TSKEAIEKKE QRAKRFHFRS
110 120 130 140 150
EVNLAQRNVA LDRDMMKKAI PKVRLETIYI CGVDEMSTQD VFSYFKEYPP
160 170 180 190 200
AHIEWLDDTS CNVVWLDEMT ATRALINMSS LPAQDKIRSR DASEDKSAEK
210 220 230 240 250
RKKDKQEDSS DDDEAEEGEV EDENSSDVEL DTLSQVEEES LLRNDLRPAN
260 270 280 290 300
KLAKGNRLFM RFATKDDKKE LGAARRSQYY MKYGNPNYGG MKGILSNSWK
310 320 330 340 350
RRYHSRRIQR DVIKKRALIG DDVGLTSYKH RHSGLVNVPE EPIEEEEEEE
360 370 380 390 400
EEEEEEEEED QDMDADDRVV VEYHEELPAL KQPRERSASR RSSASSSDSD
410 420 430 440 450
EMDYDLELKM ISTPSPKKSM KMTMYADEVE SQLKNIRNSM RADSVSSSNI
460 470 480 490 500
KNRIGNKLPP EKFADVRHLL DEKRQHSRPR PPVSSTKSDI RQRLGKRPHS
510 520 530 540 550
PEKAFSSNPV VRREPSSDVH SRLGVPRQDS KGLYADTREK KSGNLWTRLG
560 570 580 590 600
SAPKTKEKNT KKVDHRAPGA EEDDSELQRA WGALIKEKEQ SRQKKSRLDN
610 620
LPSLQIEVSR ESSSGSEAES
Length:620
Mass (Da):70,593
Last modified:October 23, 2007 - v2
Checksum:i78ABD2F3E0BE4760
GO
Isoform 2 (identifier: Q53F19-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-280: Missing.

Show »
Length:340
Mass (Da):38,920
Checksum:i7C1D9D36C19FE9B2
GO

Sequence cautioni

The sequence BAD97190 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti465D → N in BAD97190 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0289991 – 280Missing in isoform 2. 2 PublicationsAdd BLAST280

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ277841 mRNA. Translation: CAB91001.1.
AC005940 Genomic DNA. No translation available.
AC116914 Genomic DNA. No translation available.
AK125048 mRNA. Translation: BAG54129.1.
AK223470 mRNA. Translation: BAD97190.1. Different initiation.
CH471108 Genomic DNA. Translation: EAW90479.1.
BC010707 mRNA. Translation: AAH10707.1.
CCDSiCCDS45578.1. [Q53F19-1]
RefSeqiNP_001107590.1. NM_001114118.2. [Q53F19-1]
XP_016880318.1. XM_017024829.1. [Q53F19-2]
UniGeneiHs.120963.

Genome annotation databases

EnsembliENST00000389005; ENSP00000373657; ENSG00000074356. [Q53F19-1]
GeneIDi55421.
KEGGihsa:55421.
UCSCiuc010ckl.3. human. [Q53F19-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ277841 mRNA. Translation: CAB91001.1.
AC005940 Genomic DNA. No translation available.
AC116914 Genomic DNA. No translation available.
AK125048 mRNA. Translation: BAG54129.1.
AK223470 mRNA. Translation: BAD97190.1. Different initiation.
CH471108 Genomic DNA. Translation: EAW90479.1.
BC010707 mRNA. Translation: AAH10707.1.
CCDSiCCDS45578.1. [Q53F19-1]
RefSeqiNP_001107590.1. NM_001114118.2. [Q53F19-1]
XP_016880318.1. XM_017024829.1. [Q53F19-2]
UniGeneiHs.120963.

3D structure databases

ProteinModelPortaliQ53F19.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120662. 48 interactors.
IntActiQ53F19. 29 interactors.
MINTiMINT-8332606.
STRINGi9606.ENSP00000373657.

PTM databases

iPTMnetiQ53F19.
PhosphoSitePlusiQ53F19.

Polymorphism and mutation databases

BioMutaiC17orf85.
DMDMi160017360.

Proteomic databases

EPDiQ53F19.
MaxQBiQ53F19.
PaxDbiQ53F19.
PeptideAtlasiQ53F19.
PRIDEiQ53F19.

Protocols and materials databases

DNASUi55421.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000389005; ENSP00000373657; ENSG00000074356. [Q53F19-1]
GeneIDi55421.
KEGGihsa:55421.
UCSCiuc010ckl.3. human. [Q53F19-1]

Organism-specific databases

CTDi55421.
GeneCardsiC17orf85.
H-InvDBHIX0039403.
HGNCiHGNC:24612. NCBP3.
HPAiHPA008959.
HPA013195.
MIMi616624. gene.
neXtProtiNX_Q53F19.
OpenTargetsiENSG00000074356.
PharmGKBiPA145149545.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IHKG. Eukaryota.
ENOG4110MQP. LUCA.
GeneTreeiENSGT00390000005712.
HOGENOMiHOG000059260.
HOVERGENiHBG059983.
InParanoidiQ53F19.
OMAiIFSYFKE.
OrthoDBiEOG091G0ELU.
PhylomeDBiQ53F19.
TreeFamiTF331339.

Miscellaneous databases

ChiTaRSiC17orf85. human.
GenomeRNAii55421.
PROiQ53F19.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000074356.
CleanExiHS_C17orf85.
ExpressionAtlasiQ53F19. baseline and differential.
GenevisibleiQ53F19. HS.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR019416. NCBP3.
IPR012677. Nucleotide-bd_a/b_plait.
[Graphical view]
PANTHERiPTHR16291. PTHR16291. 2 hits.
PfamiPF10309. NCBP3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNCBP3_HUMAN
AccessioniPrimary (citable) accession number: Q53F19
Secondary accession number(s): B3KWG7
, Q7L406, Q96FK1, Q9NXZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: November 30, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.