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UniProtKB - Q53EZ4 (CEP55_HUMAN)

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Protein

Centrosomal protein of 55 kDa

Gene

CEP55

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in mitotic exit and cytokinesis. Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Required for successful completion of cytokinesis. Not required for microtubule nucleation.2 Publications

GO - Biological processi

  • cell separation after cytokinesis Source: UniProtKB
  • establishment of protein localization Source: UniProtKB
  • mitotic cytokinesis Source: MGI
Complete GO annotation...

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

SIGNORiQ53EZ4.

Names & Taxonomyi

Protein namesi
Recommended name:
Centrosomal protein of 55 kDa
Short name:
Cep55
Alternative name(s):
Up-regulated in colon cancer 6
Gene namesi
Name:CEP55
Synonyms:C10orf3, URCC6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:1161. CEP55.

Subcellular locationi

GO - Cellular componenti

  • centriole Source: UniProtKB-SubCell
  • centrosome Source: UniProtKB
  • cleavage furrow Source: UniProtKB-SubCell
  • Flemming body Source: UniProtKB
  • intercellular bridge Source: Ensembl
  • membrane Source: UniProtKB
  • microtubule organizing center Source: HPA
  • midbody Source: UniProtKB
  • plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi184W → A: Abolishes interaction with PDCD6IP. 1 Publication1
Mutagenesisi187Y → A: Abolishes interaction with PDCD6IP. 1 Publication1
Mutagenesisi188D → A: Diminishes interaction with PDCD6IP. 1 Publication1
Mutagenesisi191R → A: Abolishes interaction with PDCD6IP. 1 Publication1
Mutagenesisi192E → A: Abolishes interaction with PDCD6IP. 1 Publication1
Mutagenesisi396S → A: No effect on phosphorylation in mitotic cells. 1 Publication1
Mutagenesisi425S → A: Partial loss of phosphorylation in mitotic cells. Complete loss of phosphorylation in mitotic cells; when associated with A-428. Remains associated with the centrosome throughout mitosis; when associated with A-428. Arrests mitotic cells at the midbody stage; when associated with A-428 and A-436. 1 Publication1
Mutagenesisi428S → A: Partial loss of phosphorylation in mitotic cells. Complete loss of phosphorylation in mitotic cells; when associated with A-425. Remains associated with the centrosome throughout mitosis; when associated with A-425. Arrests mitotic cells at the midbody stage; when associated with A-425 and A-436. 1 Publication1
Mutagenesisi436S → A: No effect on phosphorylation in mitotic cells. Arrests mitotic cells at the midbody stage; when associated with A-425 and A-428. 1 Publication1

Organism-specific databases

DisGeNETi55165.
OpenTargetsiENSG00000138180.
PharmGKBiPA25475.

Polymorphism and mutation databases

BioMutaiCEP55.
DMDMi296439403.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000897771 – 464Centrosomal protein of 55 kDaAdd BLAST464

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei96PhosphoserineBy similarity1
Modified residuei425Phosphoserine; by CDK1 and MAPK1Combined sources1 Publication1
Modified residuei428Phosphoserine; by CDK1 and MAPK1Combined sources1 Publication1
Modified residuei430PhosphothreonineCombined sources1
Modified residuei436Phosphoserine; by PLK1Combined sources1 Publication1

Post-translational modificationi

There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ53EZ4.
MaxQBiQ53EZ4.
PaxDbiQ53EZ4.
PeptideAtlasiQ53EZ4.
PRIDEiQ53EZ4.

PTM databases

iPTMnetiQ53EZ4.
PhosphoSitePlusiQ53EZ4.

Expressioni

Tissue specificityi

Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain.2 Publications

Gene expression databases

BgeeiENSG00000138180.
CleanExiHS_CEP55.
ExpressionAtlasiQ53EZ4. baseline and differential.
GenevisibleiQ53EZ4. HS.

Organism-specific databases

HPAiHPA023430.

Interactioni

Subunit structurei

Homodimer (PubMed:16406728). Interacts (phosphorylated on Ser-425 and Ser-428) with PLK1 (PubMed:16198290). Interacts with AKAP9/CG-NAP; the interaction occurs in interphase and is lost upon mitotic entry (PubMed:16198290). Interacts with PCNT/Kendrin; the interaction occurs in interphase and is lost upon mitotic entry (PubMed:16198290). Directly interacts with PDCD6IP; this interaction is required for PDCD6IP targeting to the midbody; CEP55 binds PDCD6IP in a 2:1 stoichiometry; PDCD6IP competes with TSG101 for the same binding site (PubMed:17853893, PubMed:17556548, PubMed:18641129, PubMed:18948538). Interacts with TSG101; TSG101 competes with PDCD6IP for the same binding site; interaction is required for cytokinesis but not for viral budding (PubMed:17853893, PubMed:17556548, PubMed:18948538). Interacts with MVB12A, VPS37B, VPS37C and VPS28 (PubMed:17853893).6 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi120465. 102 interactors.
DIPiDIP-44581N.
IntActiQ53EZ4. 84 interactors.
MINTiMINT-4994059.
STRINGi9606.ENSP00000360540.

Structurei

Secondary structure

1464
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi166 – 209Combined sources44

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3E1RX-ray2.00A/B160-217[»]
3WUTX-ray2.30A/B/D/E/G/H/J/K160-217[»]
3WUUX-ray2.90A/B/D/E/G/H/J/K160-217[»]
3WUVX-ray2.79A/B/D/E/G/H/J/K/M/N/P/Q160-217[»]
ProteinModelPortaliQ53EZ4.
SMRiQ53EZ4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ53EZ4.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni157 – 236Interaction with TSG101Add BLAST80
Regioni160 – 214Interaction with PDCD6IPAdd BLAST55
Regioni355 – 464Required for localization to the interphase centrosome and to the midbody during cytokinesis1 PublicationAdd BLAST110

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili22 – 186Sequence analysisAdd BLAST165
Coiled coili238 – 337Sequence analysisAdd BLAST100
Coiled coili374 – 403Sequence analysisAdd BLAST30

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFBZ. Eukaryota.
ENOG41120Y2. LUCA.
GeneTreeiENSGT00510000047961.
HOVERGENiHBG081092.
InParanoidiQ53EZ4.
KOiK16456.
OMAiYDQQREV.
OrthoDBiEOG091G065Y.
PhylomeDBiQ53EZ4.
TreeFamiTF331107.

Family and domain databases

InterProiView protein in InterPro
IPR022008. EABR.
PfamiView protein in Pfam
PF12180. EABR. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q53EZ4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSRSTKDLI KSKWGSKPSN SKSETTLEKL KGEIAHLKTS VDEITSGKGK 
        60         70         80         90        100
LTDKERHRLL EKIRVLEAEK EKNAYQLTEK DKEIQRLRDQ LKARYSTTTL 
       110        120        130        140        150
LEQLEETTRE GERREQVLKA LSEEKDVLKQ QLSAATSRIA ELESKTNTLR 
       160        170        180        190        200
LSQTVAPNCF NSSINNIHEM EIQLKDALEK NQQWLVYDQQ REVYVKGLLA 
       210        220        230        240        250
KIFELEKKTE TAAHSLPQQT KKPESEGYLQ EEKQKCYNDL LASAKKDLEV 
       260        270        280        290        300
ERQTITQLSF ELSEFRRKYE ETQKEVHNLN QLLYSQRRAD VQHLEDDRHK 
       310        320        330        340        350
TEKIQKLREE NDIARGKLEE EKKRSEELLS QVQFLYTSLL KQQEEQTRVA 
       360        370        380        390        400
LLEQQMQACT LDFENEKLDR QHVQHQLHVI LKELRKARNQ ITQLESLKQL 
       410        420        430        440        450
HEFAITEPLV TFQGETENRE KVAASPKSPT AALNESLVEC PKCNIQYPAT 
       460 
EHRDLLVHVE YCSK
Length:464
Mass (Da):54,178
Last modified:May 18, 2010 - v3
Checksum:iA652F86EBCE46A51
GO
Isoform 2 (identifier: Q53EZ4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     389-400: NQITQLESLKQL → KNNTVGILETAS
     401-464: Missing.

Note: No experimental confirmation available.
Show »
Length:400
Mass (Da):46,857
Checksum:i61FCFE0A0965559F
GO

Sequence cautioni

The sequence BAA91670 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti155V → A in BAD97215 (Ref. 4) Curated1
Sequence conflicti204E → G in BAD97215 (Ref. 4) Curated1
Sequence conflicti298R → G in CAE45837 (PubMed:17974005).Curated1
Sequence conflicti334F → S in AAX14687 (PubMed:16406728).Curated1
Sequence conflicti334F → S in BAA91670 (PubMed:14702039).Curated1
Sequence conflicti435E → G in AAX14687 (PubMed:16406728).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02655957H → Q2 PublicationsCorresponds to variant dbSNP:rs3740370Ensembl.1
Natural variantiVAR_02299699T → ACombined sources7 PublicationsCorresponds to variant dbSNP:rs7080916Ensembl.1
Natural variantiVAR_056791236C → R. Corresponds to variant dbSNP:rs7072484Ensembl.1
Natural variantiVAR_022997378H → L4 PublicationsCorresponds to variant dbSNP:rs2293277Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014750389 – 400NQITQ…SLKQL → KNNTVGILETAS in isoform 2. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_014751401 – 464Missing in isoform 2. 1 PublicationAdd BLAST64

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY788918 mRNA. Translation: AAX14687.1.
AB091343 mRNA. Translation: BAE45243.1.
AK001402 mRNA. Translation: BAA91670.1. Different initiation.
AK315536 mRNA. Translation: BAG37915.1.
AK223495 mRNA. Translation: BAD97215.1.
BX640718 mRNA. Translation: CAE45837.1.
AL356214 Genomic DNA. Translation: CAH72324.1.
CH471066 Genomic DNA. Translation: EAW50071.1.
CH471066 Genomic DNA. Translation: EAW50072.1.
BC008947 mRNA. Translation: AAH08947.1.
CCDSiCCDS7428.1. [Q53EZ4-1]
RefSeqiNP_001120654.1. NM_001127182.1.
NP_060601.3. NM_018131.4.
UniGeneiHs.14559.

Genome annotation databases

EnsembliENST00000371485; ENSP00000360540; ENSG00000138180. [Q53EZ4-1]
GeneIDi55165.
KEGGihsa:55165.
UCSCiuc001kiq.4. human. [Q53EZ4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCEP55_HUMAN
AccessioniPrimary (citable) accession number: Q53EZ4
Secondary accession number(s): B2RDG8
, D3DR37, Q32WF5, Q3MV20, Q5VY28, Q6N034, Q96H32, Q9NVS7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: May 18, 2010
Last modified: July 5, 2017
This is version 127 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references