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Q53EU6 (GPAT3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol-3-phosphate acyltransferase 3

Short name=GPAT-3
EC=2.3.1.15
Alternative name(s):
1-acyl-sn-glycerol-3-phosphate O-acyltransferase 10
Short name=AGPAT 10
1-acyl-sn-glycerol-3-phosphate O-acyltransferase 9
Short name=1-AGP acyltransferase 9
Short name=1-AGPAT 9
EC=2.3.1.51
Acyl-CoA:glycerol-3-phosphate acyltransferase 3
Short name=hGPAT3
Lung cancer metastasis-associated protein 1
Lysophosphatidic acid acyltransferase theta
Short name=LPAAT-theta
MAG-1
Gene names
Name:AGPAT9
Synonyms:GPAT3, MAG1
ORF Names:HMFN0839, UNQ2753/PRO6492
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length434 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May transfer the acyl-group from acyl-coA to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Also transfers the acyl-group from acyl-coA to the sn-2 position of 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid, or LPA), forming 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid, or PA). Ref.2 Ref.8 Ref.10

Catalytic activity

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. Ref.2 Ref.8 Ref.10

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate. Ref.2 Ref.8 Ref.10

Enzyme regulation

Inhibited by N-ethylmaleimide (NEM). Ref.8

Pathway

Glycerolipid metabolism; triacylglycerol biosynthesis. Ref.2 Ref.10

Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. Ref.2 Ref.10

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.2 Ref.10.

Tissue specificity

Widely expressed. Expressed in liver, kidney, testis, brain, heart, skeletal muscle, thyroid, prostate, thymus and placenta. Also expressed lung and adipose tissue. Ref.2 Ref.8

Domain

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.

Sequence similarities

Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Caution

Despite its name, this protein appears to lack measurable glycerol-3-phosphate acyltransferase activity under some conditions (PMID:19318427).

Ontologies

Keywords
   Biological processLipid biosynthesis
Lipid metabolism
Phospholipid biosynthesis
Phospholipid metabolism
   Cellular componentEndoplasmic reticulum
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionAcyltransferase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processCDP-diacylglycerol biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

cellular lipid metabolic process

Traceable author statement. Source: Reactome

glycerophospholipid biosynthetic process

Traceable author statement. Source: Reactome

phosphatidic acid biosynthetic process

Traceable author statement. Source: Reactome

phospholipid metabolic process

Traceable author statement. Source: Reactome

regulation of TOR signaling

Inferred from direct assay Ref.2. Source: UniProtKB

small molecule metabolic process

Traceable author statement. Source: Reactome

triglyceride biosynthetic process

Inferred from direct assay Ref.8. Source: UniProtKB

   Cellular_componentendoplasmic reticulum

Inferred from direct assay Ref.8. Source: UniProtKB

endoplasmic reticulum membrane

Inferred from direct assay Ref.2. Source: UniProtKB

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_function1-acylglycerol-3-phosphate O-acyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

glycerol-3-phosphate O-acyltransferase activity

Inferred from direct assay Ref.8. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 434434Glycerol-3-phosphate acyltransferase 3
PRO_0000291570

Regions

Transmembrane14 – 3421Helical; Potential
Transmembrane137 – 15721Helical; Potential
Transmembrane161 – 18121Helical; Potential
Motif229 – 2346HXXXXD motif

Amino acid modifications

Modified residue681Phosphoserine Ref.9
Modified residue771Phosphoserine Ref.9

Experimental info

Sequence conflict1561L → I in BAD97263. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q53EU6 [UniParc].

Last modified June 26, 2007. Version 2.
Checksum: 80DD5423E5E7847A

FASTA43448,705
        10         20         30         40         50         60 
MEGAELAGKI LSTWLTLVLG FILLPSVFGV SLGISEIYMK ILVKTLEWAT IRIEKGTPKE 

        70         80         90        100        110        120 
SILKNSASVG IIQRDESPME KGLSGLRGRD FELSDVFYFS KKGLEAIVED EVTQRFSSEE 

       130        140        150        160        170        180 
LVSWNLLTRT NVNFQYISLR LTMVWVLGVI VRYCVLLPLR VTLAFIGISL LVIGTTLVGQ 

       190        200        210        220        230        240 
LPDSSLKNWL SELVHLTCCR ICVRALSGTI HYHNKQYRPQ KGGICVANHT SPIDVLILTT 

       250        260        270        280        290        300 
DGCYAMVGQV HGGLMGIIQR AMVKACPHVW FERSEMKDRH LVTKRLKEHI ADKKKLPILI 

       310        320        330        340        350        360 
FPEGTCINNT SVMMFKKGSF EIGGTIHPVA IKYNPQFGDA FWNSSKYNMV SYLLRMMTSW 

       370        380        390        400        410        420 
AIVCDVWYMP PMTREEGEDA VQFANRVKSA IAIQGGLTEL PWDGGLKRAK VKDIFKEEQQ 

       430 
KNYSKMIVGN GSLS 

« Hide

References

« Hide 'large scale' references
[1]"Identification of metastasis associated genes MAG-1 and MAG-2."
Zhang J., Meng Y., Du Z., Chen Z., Ling X., Xu Y., Lu Y.
Zhongguo Fei Ai Za Zhi 6:460-463(2003)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Identification of a novel human lysophosphatidic acid acyltransferase, LPAAT-theta, which activates mTOR pathway."
Tang W., Yuan J., Chen X., Gu X., Luo K., Li J., Wan B., Wang Y., Yu L.
J. Biochem. Mol. Biol. 39:626-635(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[3]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[5]Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: PNS and Testis.
[7]"Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas."
Yamada S., Ohira M., Horie H., Ando K., Takayasu H., Suzuki Y., Sugano S., Hirata T., Goto T., Matsunaga T., Hiyama E., Hayashi Y., Ando H., Suita S., Kaneko M., Sasaki F., Hashizume K., Ohnuma N., Nakagawara A.
Oncogene 23:5901-5911(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 167-434.
[8]"Molecular identification of microsomal acyl-CoA:glycerol-3-phosphate acyltransferase, a key enzyme in de novo triacylglycerol synthesis."
Cao J., Li J.-L., Li D., Tobin J.F., Gimeno R.E.
Proc. Natl. Acad. Sci. U.S.A. 103:19695-19700(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ENZYME ACTIVITY, ENZYME REGULATION, TISSUE SPECIFICITY.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68 AND SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Functional characterization of the human 1-acylglycerol-3-phosphate-O-acyltransferase isoform 10/glycerol-3-phosphate acyltransferase isoform 3."
Sukumaran S., Barnes R.I., Garg A., Agarwal A.K.
J. Mol. Endocrinol. 42:469-478(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ324782 mRNA. Translation: ABC55674.1.
DQ345298 mRNA. Translation: ABC70186.1.
AY358100 mRNA. Translation: AAQ88467.1.
AK055749 mRNA. Translation: BAB71002.1.
AK223543 mRNA. Translation: BAD97263.1.
BC073136 mRNA. Translation: AAH73136.1.
BC090956 mRNA. Translation: AAH90956.1.
AB075872 mRNA. Translation: BAD38654.1.
CCDSCCDS3606.1.
RefSeqNP_001243350.1. NM_001256421.1.
NP_001243351.1. NM_001256422.1.
NP_116106.2. NM_032717.4.
UniGeneHs.99196.

3D structure databases

ProteinModelPortalQ53EU6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid124267. 2 interactions.
STRING9606.ENSP00000264409.

PTM databases

PhosphoSiteQ53EU6.

Polymorphism databases

DMDM150403919.

Proteomic databases

MaxQBQ53EU6.
PaxDbQ53EU6.
PRIDEQ53EU6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000264409; ENSP00000264409; ENSG00000138678.
ENST00000395226; ENSP00000378651; ENSG00000138678.
GeneID84803.
KEGGhsa:84803.
UCSCuc003how.4. human.

Organism-specific databases

CTD84803.
GeneCardsGC04P084457.
HGNCHGNC:28157. AGPAT9.
HPACAB033749.
HPA029414.
MIM610958. gene.
neXtProtNX_Q53EU6.
PharmGKBPA162375888.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0204.
HOGENOMHOG000265725.
InParanoidQ53EU6.
KOK13506.
OMALVRYCVL.
OrthoDBEOG70GMFG.
PhylomeDBQ53EU6.
TreeFamTF315039.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.
UniPathwayUPA00282.
UPA00557; UER00612.

Gene expression databases

BgeeQ53EU6.
CleanExHS_AGPAT9.
GenevestigatorQ53EU6.

Family and domain databases

InterProIPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamPF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTSM00563. PlsC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiAGPAT9.
GenomeRNAi84803.
NextBio74971.
PROQ53EU6.
SOURCESearch...

Entry information

Entry nameGPAT3_HUMAN
AccessionPrimary (citable) accession number: Q53EU6
Secondary accession number(s): Q68CJ4, Q6GPI6, Q96NA3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 26, 2007
Last modified: July 9, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM