Q53EU6 (GPAT3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase 3
Short name=GPAT-3
EC=2.3.1.15

Alternative name(s):
1-acylglycerol-3-phosphate O-acyltransferase 9
Short name=1-AGP acyltransferase 9
Short name=1-AGPAT 9
Acyl-CoA:glycerol-3-phosphate acyltransferase 3
Short name=hGPAT3
Lung cancer metastasis-associated protein 1
Lysophosphatidic acid acyltransferase theta
Short name=LPAAT-theta
MAG-1

Gene names

Name:	AGPAT9
Synonyms:	GPAT3, MAG1
ORF Names:	HMFN0839, UNQ2753/PRO6492

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Overexpression activates the mTOR pathway. Ref.2 Ref.8
Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. Ref.8
Enzyme regulation	Inhibited by N-ethylmaleimide (NEM). Ref.8
Pathway	Glycerolipid metabolism; triacylglycerol biosynthesis. Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.2.
Tissue specificity	Widely expressed. Expressed in liver, kidney, testis, brain, heart, skeletal muscle, thyroid, prostate, thymus and placenta. Also expressed lung and adipose tissue. Ref.2 Ref.8
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CDP-diacylglycerol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway phosphatidic acid biosynthetic process Traceable author statement. Source: Reactome regulation of TOR signaling cascade Inferred from direct assay Ref.2. Source: UniProtKB small molecule metabolic process Traceable author statement. Source: Reactome triglyceride biosynthetic process Inferred from direct assay Ref.8. Source: UniProtKB
Cellular_component	endoplasmic reticulum membrane Inferred from direct assay Ref.2. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	glycerol-3-phosphate O-acyltransferase activity Inferred from direct assay Ref.8. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	Glycerol-3-phosphate acyltransferase 3		PRO_0000291570
Regions
	Transmembrane	14 – 34	21	Helical; Potential
	Transmembrane	137 – 157	21	Helical; Potential
	Transmembrane	161 – 181	21	Helical; Potential
	Motif	229 – 234	6	HXXXXD motif
Amino acid modifications
	Modified residue	68	1	Phosphoserine Ref.9
	Modified residue	77	1	Phosphoserine Ref.9
Experimental info
	Sequence conflict	156	1	L → I in BAD97263. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q53EU6 [UniParc]. Last modified June 26, 2007. Version 2. Checksum: 80DD5423E5E7847A	FASTA	434	48,705
	10 20 30 40 50 60 MEGAELAGKI LSTWLTLVLG FILLPSVFGV SLGISEIYMK ILVKTLEWAT IRIEKGTPKE 70 80 90 100 110 120 SILKNSASVG IIQRDESPME KGLSGLRGRD FELSDVFYFS KKGLEAIVED EVTQRFSSEE 130 140 150 160 170 180 LVSWNLLTRT NVNFQYISLR LTMVWVLGVI VRYCVLLPLR VTLAFIGISL LVIGTTLVGQ 190 200 210 220 230 240 LPDSSLKNWL SELVHLTCCR ICVRALSGTI HYHNKQYRPQ KGGICVANHT SPIDVLILTT 250 260 270 280 290 300 DGCYAMVGQV HGGLMGIIQR AMVKACPHVW FERSEMKDRH LVTKRLKEHI ADKKKLPILI 310 320 330 340 350 360 FPEGTCINNT SVMMFKKGSF EIGGTIHPVA IKYNPQFGDA FWNSSKYNMV SYLLRMMTSW 370 380 390 400 410 420 AIVCDVWYMP PMTREEGEDA VQFANRVKSA IAIQGGLTEL PWDGGLKRAK VKDIFKEEQQ 430 KNYSKMIVGN GSLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of metastasis associated genes MAG-1 and MAG-2." Zhang J., Meng Y., Du Z., Chen Z., Ling X., Xu Y., Lu Y. Zhongguo Fei Ai Za Zhi 6:460-463(2003) Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Identification of a novel human lysophosphatidic acid acyltransferase, LPAAT-theta, which activates mTOR pathway." Tang W., Yuan J., Chen X., Gu X., Luo K., Li J., Wan B., Wang Y., Yu L. J. Biochem. Mol. Biol. 39:626-635(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[5]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: PNS and Testis.
[7]	"Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas." Yamada S., Ohira M., Horie H., Ando K., Takayasu H., Suzuki Y., Sugano S., Hirata T., Goto T., Matsunaga T., Hiyama E., Hayashi Y., Ando H., Suita S., Kaneko M., Sasaki F., Hashizume K., Ohnuma N., Nakagawara A. Oncogene 23:5901-5911(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 167-434.
[8]	"Molecular identification of microsomal acyl-CoA:glycerol-3-phosphate acyltransferase, a key enzyme in de novo triacylglycerol synthesis." Cao J., Li J.-L., Li D., Tobin J.F., Gimeno R.E. Proc. Natl. Acad. Sci. U.S.A. 103:19695-19700(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ENZYME ACTIVITY, ENZYME REGULATION, TISSUE SPECIFICITY.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68 AND SER-77, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ324782 mRNA. Translation: ABC55674.1. DQ345298 mRNA. Translation: ABC70186.1. AY358100 mRNA. Translation: AAQ88467.1. AK055749 mRNA. Translation: BAB71002.1. AK223543 mRNA. Translation: BAD97263.1. BC073136 mRNA. Translation: AAH73136.1. BC090956 mRNA. Translation: AAH90956.1. AB075872 mRNA. Translation: BAD38654.1.
IPI	IPI00140301.
RefSeq	NP_001243350.1. NM_001256421.1. NP_001243351.1. NM_001256422.1. NP_116106.2. NM_032717.4.
UniGene	Hs.99196.
3D structure databases
ProteinModelPortal	Q53EU6.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000264409.
PTM databases
PhosphoSite	Q53EU6.
Polymorphism databases
DMDM	150403919.
Proteomic databases
PaxDb	Q53EU6.
PRIDE	Q53EU6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000264409; ENSP00000264409; ENSG00000138678. ENST00000395226; ENSP00000378651; ENSG00000138678.
GeneID	84803.
KEGG	hsa:84803.
UCSC	uc003how.3. human.
Organism-specific databases
CTD	84803.
GeneCards	GC04P084457.
HGNC	HGNC:28157. AGPAT9.
HPA	CAB033749. HPA029414.
MIM	610958. gene.
neXtProt	NX_Q53EU6.
PharmGKB	PA162375888.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0204.
HOGENOM	HOG000265725.
InParanoid	Q53EU6.
KO	K13506.
OMA	FERAEMK.
OrthoDB	EOG4Z62NH.
PhylomeDB	Q53EU6.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
UniPathway	UPA00282. UPA00557; UER00612.
Gene expression databases
Bgee	Q53EU6.
CleanEx	HS_AGPAT9.
Genevestigator	Q53EU6.
Family and domain databases
InterPro	IPR002123. Plipid/glycerol_acylTrfase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	84803.
NextBio	74971.
SOURCE	Search...

Entry information

Entry name

GPAT3_HUMAN

Accession

Primary (citable) accession number: Q53EU6
Secondary accession number(s): Q68CJ4, Q6GPI6, Q96NA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	June 26, 2007
Last modified:	April 3, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.