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Q53EU6

- GPAT3_HUMAN

UniProt

Q53EU6 - GPAT3_HUMAN

Protein

Glycerol-3-phosphate acyltransferase 3

Gene

AGPAT9

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 2 (26 Jun 2007)
      Previous versions | rss
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    Functioni

    May transfer the acyl-group from acyl-coA to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Also transfers the acyl-group from acyl-coA to the sn-2 position of 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid, or LPA), forming 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid, or PA).3 Publications

    Catalytic activityi

    Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.
    Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.

    Enzyme regulationi

    Inhibited by N-ethylmaleimide (NEM).1 Publication

    Pathwayi

    GO - Molecular functioni

    1. 1-acylglycerol-3-phosphate O-acyltransferase activity Source: UniProtKB-EC
    2. glycerol-3-phosphate O-acyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. CDP-diacylglycerol biosynthetic process Source: UniProtKB-UniPathway
    2. cellular lipid metabolic process Source: Reactome
    3. glycerophospholipid biosynthetic process Source: Reactome
    4. phosphatidic acid biosynthetic process Source: Reactome
    5. phospholipid metabolic process Source: Reactome
    6. regulation of TOR signaling Source: UniProtKB
    7. small molecule metabolic process Source: Reactome
    8. triglyceride biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Enzyme and pathway databases

    ReactomeiREACT_1190. Triglyceride Biosynthesis.
    REACT_120906. Synthesis of PA.
    UniPathwayiUPA00282.
    UPA00557; UER00612.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerol-3-phosphate acyltransferase 3 (EC:2.3.1.15)
    Short name:
    GPAT-3
    Alternative name(s):
    1-acyl-sn-glycerol-3-phosphate O-acyltransferase 10
    Short name:
    AGPAT 10
    1-acyl-sn-glycerol-3-phosphate O-acyltransferase 9 (EC:2.3.1.51)
    Short name:
    1-AGP acyltransferase 9
    Short name:
    1-AGPAT 9
    Acyl-CoA:glycerol-3-phosphate acyltransferase 3
    Short name:
    hGPAT3
    Lung cancer metastasis-associated protein 1
    Lysophosphatidic acid acyltransferase theta
    Short name:
    LPAAT-theta
    MAG-1
    Gene namesi
    Name:AGPAT9
    Synonyms:GPAT3, MAG1
    ORF Names:HMFN0839, UNQ2753/PRO6492
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:28157. AGPAT9.

    Subcellular locationi

    Endoplasmic reticulum membrane 2 Publications; Multi-pass membrane protein 2 Publications

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. endoplasmic reticulum membrane Source: UniProtKB
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162375888.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 434434Glycerol-3-phosphate acyltransferase 3PRO_0000291570Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei68 – 681Phosphoserine1 Publication
    Modified residuei77 – 771Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ53EU6.
    PaxDbiQ53EU6.
    PRIDEiQ53EU6.

    PTM databases

    PhosphoSiteiQ53EU6.

    Expressioni

    Tissue specificityi

    Widely expressed. Expressed in liver, kidney, testis, brain, heart, skeletal muscle, thyroid, prostate, thymus and placenta. Also expressed lung and adipose tissue.2 Publications

    Gene expression databases

    BgeeiQ53EU6.
    CleanExiHS_AGPAT9.
    GenevestigatoriQ53EU6.

    Organism-specific databases

    HPAiCAB033749.
    HPA029414.

    Interactioni

    Protein-protein interaction databases

    BioGridi124267. 2 interactions.
    STRINGi9606.ENSP00000264409.

    Structurei

    3D structure databases

    ProteinModelPortaliQ53EU6.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei14 – 3421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei137 – 15721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei161 – 18121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi229 – 2346HXXXXD motif

    Domaini

    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0204.
    HOGENOMiHOG000265725.
    InParanoidiQ53EU6.
    KOiK13506.
    OMAiLVRYCVL.
    OrthoDBiEOG70GMFG.
    PhylomeDBiQ53EU6.
    TreeFamiTF315039.

    Family and domain databases

    InterProiIPR002123. Plipid/glycerol_acylTrfase.
    [Graphical view]
    PfamiPF01553. Acyltransferase. 1 hit.
    [Graphical view]
    SMARTiSM00563. PlsC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q53EU6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGAELAGKI LSTWLTLVLG FILLPSVFGV SLGISEIYMK ILVKTLEWAT    50
    IRIEKGTPKE SILKNSASVG IIQRDESPME KGLSGLRGRD FELSDVFYFS 100
    KKGLEAIVED EVTQRFSSEE LVSWNLLTRT NVNFQYISLR LTMVWVLGVI 150
    VRYCVLLPLR VTLAFIGISL LVIGTTLVGQ LPDSSLKNWL SELVHLTCCR 200
    ICVRALSGTI HYHNKQYRPQ KGGICVANHT SPIDVLILTT DGCYAMVGQV 250
    HGGLMGIIQR AMVKACPHVW FERSEMKDRH LVTKRLKEHI ADKKKLPILI 300
    FPEGTCINNT SVMMFKKGSF EIGGTIHPVA IKYNPQFGDA FWNSSKYNMV 350
    SYLLRMMTSW AIVCDVWYMP PMTREEGEDA VQFANRVKSA IAIQGGLTEL 400
    PWDGGLKRAK VKDIFKEEQQ KNYSKMIVGN GSLS 434
    Length:434
    Mass (Da):48,705
    Last modified:June 26, 2007 - v2
    Checksum:i80DD5423E5E7847A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti156 – 1561L → I in BAD97263. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ324782 mRNA. Translation: ABC55674.1.
    DQ345298 mRNA. Translation: ABC70186.1.
    AY358100 mRNA. Translation: AAQ88467.1.
    AK055749 mRNA. Translation: BAB71002.1.
    AK223543 mRNA. Translation: BAD97263.1.
    BC073136 mRNA. Translation: AAH73136.1.
    BC090956 mRNA. Translation: AAH90956.1.
    AB075872 mRNA. Translation: BAD38654.1.
    CCDSiCCDS3606.1.
    RefSeqiNP_001243350.1. NM_001256421.1.
    NP_001243351.1. NM_001256422.1.
    NP_116106.2. NM_032717.4.
    UniGeneiHs.99196.

    Genome annotation databases

    EnsembliENST00000264409; ENSP00000264409; ENSG00000138678.
    ENST00000395226; ENSP00000378651; ENSG00000138678.
    GeneIDi84803.
    KEGGihsa:84803.
    UCSCiuc003how.4. human.

    Polymorphism databases

    DMDMi150403919.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ324782 mRNA. Translation: ABC55674.1 .
    DQ345298 mRNA. Translation: ABC70186.1 .
    AY358100 mRNA. Translation: AAQ88467.1 .
    AK055749 mRNA. Translation: BAB71002.1 .
    AK223543 mRNA. Translation: BAD97263.1 .
    BC073136 mRNA. Translation: AAH73136.1 .
    BC090956 mRNA. Translation: AAH90956.1 .
    AB075872 mRNA. Translation: BAD38654.1 .
    CCDSi CCDS3606.1.
    RefSeqi NP_001243350.1. NM_001256421.1.
    NP_001243351.1. NM_001256422.1.
    NP_116106.2. NM_032717.4.
    UniGenei Hs.99196.

    3D structure databases

    ProteinModelPortali Q53EU6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 124267. 2 interactions.
    STRINGi 9606.ENSP00000264409.

    PTM databases

    PhosphoSitei Q53EU6.

    Polymorphism databases

    DMDMi 150403919.

    Proteomic databases

    MaxQBi Q53EU6.
    PaxDbi Q53EU6.
    PRIDEi Q53EU6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000264409 ; ENSP00000264409 ; ENSG00000138678 .
    ENST00000395226 ; ENSP00000378651 ; ENSG00000138678 .
    GeneIDi 84803.
    KEGGi hsa:84803.
    UCSCi uc003how.4. human.

    Organism-specific databases

    CTDi 84803.
    GeneCardsi GC04P084457.
    HGNCi HGNC:28157. AGPAT9.
    HPAi CAB033749.
    HPA029414.
    MIMi 610958. gene.
    neXtProti NX_Q53EU6.
    PharmGKBi PA162375888.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0204.
    HOGENOMi HOG000265725.
    InParanoidi Q53EU6.
    KOi K13506.
    OMAi LVRYCVL.
    OrthoDBi EOG70GMFG.
    PhylomeDBi Q53EU6.
    TreeFami TF315039.

    Enzyme and pathway databases

    UniPathwayi UPA00282 .
    UPA00557 ; UER00612 .
    Reactomei REACT_1190. Triglyceride Biosynthesis.
    REACT_120906. Synthesis of PA.

    Miscellaneous databases

    GeneWikii AGPAT9.
    GenomeRNAii 84803.
    NextBioi 74971.
    PROi Q53EU6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q53EU6.
    CleanExi HS_AGPAT9.
    Genevestigatori Q53EU6.

    Family and domain databases

    InterProi IPR002123. Plipid/glycerol_acylTrfase.
    [Graphical view ]
    Pfami PF01553. Acyltransferase. 1 hit.
    [Graphical view ]
    SMARTi SM00563. PlsC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of metastasis associated genes MAG-1 and MAG-2."
      Zhang J., Meng Y., Du Z., Chen Z., Ling X., Xu Y., Lu Y.
      Zhongguo Fei Ai Za Zhi 6:460-463(2003)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Identification of a novel human lysophosphatidic acid acyltransferase, LPAAT-theta, which activates mTOR pathway."
      Tang W., Yuan J., Chen X., Gu X., Luo K., Li J., Wan B., Wang Y., Yu L.
      J. Biochem. Mol. Biol. 39:626-635(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    5. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
      Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: PNS and Testis.
    7. "Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas."
      Yamada S., Ohira M., Horie H., Ando K., Takayasu H., Suzuki Y., Sugano S., Hirata T., Goto T., Matsunaga T., Hiyama E., Hayashi Y., Ando H., Suita S., Kaneko M., Sasaki F., Hashizume K., Ohnuma N., Nakagawara A.
      Oncogene 23:5901-5911(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 167-434.
    8. "Molecular identification of microsomal acyl-CoA:glycerol-3-phosphate acyltransferase, a key enzyme in de novo triacylglycerol synthesis."
      Cao J., Li J.-L., Li D., Tobin J.F., Gimeno R.E.
      Proc. Natl. Acad. Sci. U.S.A. 103:19695-19700(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME ACTIVITY, ENZYME REGULATION, TISSUE SPECIFICITY.
    9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68 AND SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. "Functional characterization of the human 1-acylglycerol-3-phosphate-O-acyltransferase isoform 10/glycerol-3-phosphate acyltransferase isoform 3."
      Sukumaran S., Barnes R.I., Garg A., Agarwal A.K.
      J. Mol. Endocrinol. 42:469-478(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiGPAT3_HUMAN
    AccessioniPrimary (citable) accession number: Q53EU6
    Secondary accession number(s): Q68CJ4, Q6GPI6, Q96NA3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 26, 2007
    Last sequence update: June 26, 2007
    Last modified: October 1, 2014
    This is version 81 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    Despite its name, this protein appears to lack measurable glycerol-3-phosphate acyltransferase activity under some conditions (PMID:19318427).Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3