ID   RAB43_RAT               Reviewed;         210 AA.
AC   Q53B90;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   27-MAR-2024, entry version 129.
DE   RecName: Full=Ras-related protein Rab-43;
GN   Name=Rab43;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley;
RA   Zhong C., Zhou G., Ji G., Yu L.;
RT   "Cloning, expression pattern and homology modeling of novel rat RAB
RT   genes.";
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The small GTPases Rab are key regulators of intracellular
CC       membrane trafficking, from the formation of transport vesicles to their
CC       fusion with membranes. Rabs cycle between an inactive GDP-bound form
CC       and an active GTP-bound form that is able to recruit to membranes
CC       different set of downstream effectors directly responsible for vesicle
CC       formation, movement, tethering and fusion. The low intrinsic GTPase
CC       activity of RAB43 is activated by USP6NL. Involved in retrograde
CC       transport from the endocytic pathway to the Golgi apparatus. Involved
CC       in the transport of Shiga toxin from early and recycling endosomes to
CC       the trans-Golgi network. Required for the structural integrity of the
CC       Golgi complex. Plays a role in the maturation of phagosomes that engulf
CC       pathogens, such as S.aureus and Mycobacterium.
CC   -!- SUBUNIT: Interacts with GDI1, GDI2 and CHM; phosphorylation at Thr-80
CC       disrupts these interactions. {ECO:0000250|UniProtKB:Q86YS6}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, phagosome
CC       {ECO:0000250|UniProtKB:Q86YS6}. Cytoplasmic vesicle, phagosome membrane
CC       {ECO:0000305}; Lipid-anchor {ECO:0000305}; Cytoplasmic side
CC       {ECO:0000305}. Golgi apparatus {ECO:0000250|UniProtKB:Q86YS6}. Golgi
CC       apparatus, trans-Golgi network membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000305}. Golgi apparatus, trans-Golgi network
CC       {ECO:0000250|UniProtKB:Q86YS6}. Note=Recruited to phagosomes containing
CC       S.aureus or M.tuberculosis. {ECO:0000250|UniProtKB:Q86YS6}.
CC   -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC       {ECO:0000305}.
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DR   EMBL; AY593256; AAT86135.1; -; mRNA.
DR   RefSeq; NP_001019502.1; NM_001024331.1.
DR   AlphaFoldDB; Q53B90; -.
DR   SMR; Q53B90; -.
DR   STRING; 10116.ENSRNOP00000013299; -.
DR   PhosphoSitePlus; Q53B90; -.
DR   jPOST; Q53B90; -.
DR   PaxDb; 10116-ENSRNOP00000013299; -.
DR   Ensembl; ENSRNOT00000013299.5; ENSRNOP00000013299.4; ENSRNOG00000037768.3.
DR   Ensembl; ENSRNOT00055022211; ENSRNOP00055018048; ENSRNOG00055012969.
DR   Ensembl; ENSRNOT00060041918; ENSRNOP00060034762; ENSRNOG00060024176.
DR   Ensembl; ENSRNOT00065024961; ENSRNOP00065019531; ENSRNOG00065015079.
DR   GeneID; 500249; -.
DR   KEGG; rno:500249; -.
DR   UCSC; RGD:1565300; rat.
DR   AGR; RGD:1565300; -.
DR   CTD; 339122; -.
DR   RGD; 1565300; Rab43.
DR   eggNOG; KOG0084; Eukaryota.
DR   GeneTree; ENSGT00940000161980; -.
DR   HOGENOM; CLU_041217_23_1_1; -.
DR   InParanoid; Q53B90; -.
DR   OMA; FIERHGN; -.
DR   OrthoDB; 8685at2759; -.
DR   PhylomeDB; Q53B90; -.
DR   TreeFam; TF300097; -.
DR   Reactome; R-RNO-6811440; Retrograde transport at the Trans-Golgi-Network.
DR   Reactome; R-RNO-8873719; RAB geranylgeranylation.
DR   PRO; PR:Q53B90; -.
DR   Proteomes; UP000002494; Chromosome 4.
DR   Bgee; ENSRNOG00000037768; Expressed in liver and 18 other cell types or tissues.
DR   GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR   GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR   GO; GO:0045335; C:phagocytic vesicle; ISS:UniProtKB.
DR   GO; GO:0030670; C:phagocytic vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; ISS:UniProtKB.
DR   GO; GO:0000045; P:autophagosome assembly; IBA:GO_Central.
DR   GO; GO:0071346; P:cellular response to type II interferon; ISO:RGD.
DR   GO; GO:0007030; P:Golgi organization; ISS:UniProtKB.
DR   GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR   GO; GO:0090382; P:phagosome maturation; ISS:UniProtKB.
DR   GO; GO:0035526; P:retrograde transport, plasma membrane to Golgi; ISS:UniProtKB.
DR   GO; GO:0019068; P:virion assembly; IEA:Ensembl.
DR   CDD; cd01864; Rab19; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR048040; Rab19/43.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR001806; Small_GTPase.
DR   NCBIfam; TIGR00231; small_GTP; 1.
DR   PANTHER; PTHR47979; DRAB11-RELATED; 1.
DR   PANTHER; PTHR47979:SF117; RAB43, MEMBER RAS ONCOGENE FAMILY; 1.
DR   Pfam; PF00071; Ras; 1.
DR   PRINTS; PR00449; RASTRNSFRMNG.
DR   SMART; SM00175; RAB; 1.
DR   SMART; SM00176; RAN; 1.
DR   SMART; SM00173; RAS; 1.
DR   SMART; SM00174; RHO; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS51419; RAB; 1.
DR   Genevisible; Q53B90; RN.
PE   2: Evidence at transcript level;
KW   Cytoplasmic vesicle; Golgi apparatus; GTP-binding; Lipoprotein; Membrane;
KW   Methylation; Nucleotide-binding; Phosphoprotein; Prenylation;
KW   Reference proteome.
FT   CHAIN           1..210
FT                   /note="Ras-related protein Rab-43"
FT                   /id="PRO_0000244617"
FT   MOTIF           45..53
FT                   /note="Effector region"
FT                   /evidence="ECO:0000250"
FT   BINDING         23..30
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         71..75
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         129..132
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         161..162
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         47
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8CG50"
FT   MOD_RES         80
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q86YS6"
FT   MOD_RES         210
FT                   /note="Cysteine methyl ester"
FT                   /evidence="ECO:0000250"
FT   LIPID           208
FT                   /note="S-geranylgeranyl cysteine"
FT                   /evidence="ECO:0000250"
FT   LIPID           210
FT                   /note="S-geranylgeranyl cysteine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   210 AA;  23229 MW;  4D7DF08F84AE47A0 CRC64;
     MAGPGPGDQD EHYDFLFKLV LVGDASVGKT CVVQRFKTGA FSARQGSTIG VDFTMKTLEI
     QGKRVKLQIW DTAGQERFRT ITQSYYRSAN GAILAYDISK RSTFLSVPHW IEDVRKYAGS
     NIVQLLIGNK SDLADLREVP LAEAQSLAEH YDILCAIETS AKDSSNVEEA FTRVATELIM
     RHGGPMFSEK NTDHIQLDSK DIAESWGCGC
//