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Protein

Transmembrane and ubiquitin-like domain-containing protein 1

Gene

Tmub1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in sterol-regulated ubiquitination and degradation of HMG-CoA reductase HMGCR (By similarity). Involved in positive regulation of AMPA-selective glutamate receptor GRIA2 recycling to the cell surface (PubMed:18665261). Acts as negative regulator of hepatocyte growth during regeneration (PubMed:22426572).By similarity2 Publications
iHOPS: May contribute to the regulation of translation during cell-cycle progression. May contribute to the regulation of cell proliferation (By similarity). May be involved in centrosome assembly. Modulates stabilization and nucleolar localization of tumor suppressor CDKN2A and enhances association between CDKN2A and NPM1 (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane and ubiquitin-like domain-containing protein 1
Alternative name(s):
Hepatocyte odd protein shuttling protein
Cleaved into the following chain:
iHOPS1 Publication
Gene namesi
Name:Tmub1
Synonyms:Hops
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi735120. Tmub1.

Subcellular locationi

iHOPS :

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei11 – 3121HelicalSequence analysisAdd
BLAST
Transmembranei194 – 21421HelicalSequence analysisAdd
BLAST
Transmembranei219 – 23921HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Endosome, Membrane, Nucleus, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 245iHOPSPRO_0000435490
Chaini1 – 245245Transmembrane and ubiquitin-like domain-containing protein 1PRO_0000370251Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei97 – 971PhosphoserineBy similarity

Post-translational modificationi

iHOPS: Processed by regulated intramembrane proteolysis (RIP) in the N-terminus to release iHOPS from membranes.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ53AQ4.
PRIDEiQ53AQ4.

PTM databases

iPTMnetiQ53AQ4.

Expressioni

Inductioni

Up-regulated in regenerating liver.1 Publication

Gene expression databases

GenevisibleiQ53AQ4. RN.

Interactioni

Subunit structurei

Interacts with EEF1A1, GRIA2, GRIP1 (By similarity). Interacts with CAMLG, TUBG1. Interacts with NPM1 and CDKN2A; TMUB1 can enhance interaction between NPM1 and CDKN2A and is proposed to bridge the proteins; proposed to be mediated by iHOPS (PubMed:18418082, PubMed:22426572, PubMed:22890319). Interacts with ERLIN2 and AMFR; TMUB1 promotes the interaction of ERLIN2 with AMFR (By similarity).By similarity3 Publications

Protein-protein interaction databases

BioGridi263349. 2 interactions.
IntActiQ53AQ4. 2 interactions.
STRINGi10116.ENSRNOP00000058703.

Structurei

3D structure databases

ProteinModelPortaliQ53AQ4.
SMRiQ53AQ4. Positions 100-172.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini102 – 17574Ubiquitin-likePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 3029Required to release iHOPS from membranesBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG1T. Eukaryota.
ENOG4111WH7. LUCA.
GeneTreeiENSGT00390000014069.
HOGENOMiHOG000232067.
InParanoidiQ53AQ4.
OMAiHQVRLIY.
OrthoDBiEOG7TF7B5.
PhylomeDBiQ53AQ4.
TreeFamiTF329265.

Family and domain databases

InterProiIPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q53AQ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALIEGVGDE VTVLFSVLAC LLVLALAWVS THTTESTDPL PQSSGTTTPA
60 70 80 90 100
QPSEAMTAID SIREEAPGAE SPSLRHRGPS AQPEPEAGVT ASTPPDSPQE
110 120 130 140 150
PLLLRLKFLN DSEQVARAWP QDTIGSLKRT QFPGREQQVR LIYQGQLLGD
160 170 180 190 200
DTQTLGSLHL PPNCVLHCHV STRVGPPHPP CPPGSEPGPS GLEIGSLLLP
210 220 230 240
LLLLLLLLLW YCQIQYRPFF PLTATLGLAG FTLLLSLLAF AMYRP
Length:245
Mass (Da):26,349
Last modified:May 24, 2005 - v1
Checksum:iA83B3C4AB6C992A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY603378 mRNA. Translation: AAU00154.1.
CH474020 Genomic DNA. Translation: EDL99355.1.
RefSeqiNP_001073622.1. NM_001080153.2.
NP_942076.2. NM_198781.3.
XP_006236007.1. XM_006235945.2.
XP_008760873.1. XM_008762651.1.
UniGeneiRn.105624.

Genome annotation databases

EnsembliENSRNOT00000061992; ENSRNOP00000058703; ENSRNOG00000013383.
ENSRNOT00000088357; ENSRNOP00000074242; ENSRNOG00000013383.
GeneIDi362301.
KEGGirno:362301.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY603378 mRNA. Translation: AAU00154.1.
CH474020 Genomic DNA. Translation: EDL99355.1.
RefSeqiNP_001073622.1. NM_001080153.2.
NP_942076.2. NM_198781.3.
XP_006236007.1. XM_006235945.2.
XP_008760873.1. XM_008762651.1.
UniGeneiRn.105624.

3D structure databases

ProteinModelPortaliQ53AQ4.
SMRiQ53AQ4. Positions 100-172.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi263349. 2 interactions.
IntActiQ53AQ4. 2 interactions.
STRINGi10116.ENSRNOP00000058703.

PTM databases

iPTMnetiQ53AQ4.

Proteomic databases

PaxDbiQ53AQ4.
PRIDEiQ53AQ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000061992; ENSRNOP00000058703; ENSRNOG00000013383.
ENSRNOT00000088357; ENSRNOP00000074242; ENSRNOG00000013383.
GeneIDi362301.
KEGGirno:362301.

Organism-specific databases

CTDi83590.
RGDi735120. Tmub1.

Phylogenomic databases

eggNOGiENOG410IG1T. Eukaryota.
ENOG4111WH7. LUCA.
GeneTreeiENSGT00390000014069.
HOGENOMiHOG000232067.
InParanoidiQ53AQ4.
OMAiHQVRLIY.
OrthoDBiEOG7TF7B5.
PhylomeDBiQ53AQ4.
TreeFamiTF329265.

Miscellaneous databases

PROiQ53AQ4.

Gene expression databases

GenevisibleiQ53AQ4. RN.

Family and domain databases

InterProiIPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "HOPS: a novel cAMP-dependent shuttling protein involved in protein synthesis regulation."
    Della Fazia M.A., Castelli M., Bartoli D., Pieroni S., Pettirossi V., Piobbico D., Viola-Magni M., Servillo G.
    J. Cell Sci. 118:3185-3194(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, SUBCELLULAR LOCATION.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  3. Cited for: SUBCELLULAR LOCATION, INTERACTION WITH TUBG1.
  4. "Transmembrane and ubiquitin-like domain-containing protein 1 (Tmub1/HOPS) facilitates surface expression of GluR2-containing AMPA receptors."
    Yang H., Takagi H., Konishi Y., Ageta H., Ikegami K., Yao I., Sato S., Hatanaka K., Inokuchi K., Seog D.H., Setou M.
    PLoS ONE 3:E2809-E2809(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "IL-6 induction of hepatocyte proliferation through the Tmub1-regulated gene pathway."
    Liu M., Liu H., Wang X., Chen P., Chen H.
    Int. J. Mol. Med. 29:1106-1112(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CAMLG.
  6. "Hepatocyte odd protein shuttling (HOPS) is a bridging protein in the nucleophosmin-p19 Arf network."
    Castelli M., Pieroni S., Brunacci C., Piobbico D., Bartoli D., Bellet M.M., Colombo E., Pelicci P.G., Della Fazia M.A., Servillo G.
    Oncogene 32:3350-3358(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH NPM1 AND CDKN2A.
  7. "Different functions of HOPS isoforms in the cell: HOPS shuttling isoform is determined by RIP cleavage system."
    Castelli M., Piobbico D., Bartoli D., Pieroni S., Brunacci C., Bellet M.M., Chiacchiaretta M., Della Fazia M.A., Servillo G.
    Cell Cycle 13:293-302(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION (IHOPS), PROTEOLYTIC CLEAVAGE (IHOPS).

Entry informationi

Entry nameiTMUB1_RAT
AccessioniPrimary (citable) accession number: Q53AQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 24, 2005
Last modified: June 8, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.