Q539E5 (APX1_HYDVD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative ascorbate peroxidase
EC=1.11.1.11

Alternative name(s):
HvAPX1

Gene names

Name:

APX1

Organism

Hydra viridissima (Green hydra) (Chlorohydra viridissima)

Taxonomic identifier

6082 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Cnidaria › Hydrozoa › Hydroida › Anthomedusae › Hydridae › Hydra

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a role in the protection of oocyte incorporated cells from rapid apoptotic degradation.
Catalytic activity	2 L-ascorbate + H2O2 + 2 H+ = L-ascorbate + L-dehydroascorbate + 2 H2O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity.
Developmental stage	Expressed exclusively during oogenesis. Ref.1
Miscellaneous	The gene for this protein may have been transferred horizontally following an endosymbiotic event early in evolution of the Hydra lineage as an RNA or cDNA intermediate.
Sequence similarities	Belongs to the peroxidase family.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological_process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	L-ascorbate peroxidase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 367	351	Putative ascorbate peroxidase		PRO_0000023637
Sites
	Active site	58	1	Proton acceptor By similarity
	Site	54	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	119	1	N-linked (GlcNAc...) Potential
	Glycosylation	239	1	N-linked (GlcNAc...) Potential
	Glycosylation	356	1	N-linked (GlcNAc...) Potential
	Disulfide bond	60 ↔ 66		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q539E5 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 91E0A494F416A10D	FASTA	367	40,790
	10 20 30 40 50 60 MVPVIWLTVL IVLVDSLQII PTFHDFQRAK TDLLGLIESV KRGDDLPMIA GTVRLAFHDC 70 80 90 100 110 120 IGKGKCDGCI DHSKPGNAGL KRVTDRLDAL YDASYKGKIS RADFYALASV TALTRSTANL 130 140 150 160 170 180 SDKYNGLRKF KVGRKDCSTS PVESIDSSDI PRGSDGTSKT LQFFKSEFGM KTQEAVALLG 190 200 210 220 230 240 AHTLGRCSLQ NSGFVGSWVD QRFSTAPPGE ENLSPTSILD NAYYRMIIDI VPWTQVNING 250 260 270 280 290 300 TRIQWQEPSN SIPNDKLPES KRSPLLLNSD MAISWIIKPS DALGTVSCRP TSLKTPCRHS 310 320 330 340 350 360 NAHTFAKIYA KNNALWVKDF TKAFNKMIEM NENKLRKAPI FNGYFDEMNS HDEPINESVD EISEDIF

References

[1]	"Symbiotic Hydra express a plant-like peroxidase gene during oogenesis." Habetha M., Bosch T.C.G. J. Exp. Biol. 208:2157-2165(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE. Strain: A99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY608909 mRNA. Translation: AAU07981.1.
3D structure databases
ProteinModelPortal	Q539E5.
ModBase	Search...
Protein family/group databases
PeroxiBase	2285. HviNAnPrx01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 1 hit. [Graphical view]
PRINTS	PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. False negative. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APX1_HYDVD

Accession

Primary (citable) accession number: Q539E5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	May 24, 2005
Last modified:	November 28, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)